Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,456,233 G→T 100% I277I (ATC→ATA)  treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,456,2330GT97.8% 869.0 / 5.9 281I277I (ATC→ATA) treRtrehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (151/123);  total (152/129)
Fisher's exact test for biased strand distribution p-value = 5.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

GAAGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAT  >  CP009273/4456086‑4456379
                                                                                                                                                   |                                                                                                                                                  
 aaGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtc                                                                                                                                                 >  1:1678080/1‑150 (MQ=255)
  aGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtct                                                                                                                                                >  1:4620511/1‑150 (MQ=255)
  aGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTAtct                                                                                                                                                >  1:1120876/1‑150 (MQ=255)
    aTCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTcc                                                                                                                                              >  2:1534986/1‑150 (MQ=255)
     tCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCg                                                                                                                                             >  2:4440771/1‑150 (MQ=255)
      ctcaATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCgg                                                                                                                                            <  1:5048623/148‑1 (MQ=255)
        caATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAt                                                                                                                                          <  1:2975583/150‑1 (MQ=255)
         aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                         >  2:3600206/1‑150 (MQ=255)
         aaTTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATg                                                                                                                                         >  2:3593599/1‑150 (MQ=255)
            ttATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggag                                                                                                                                      >  2:1975726/1‑150 (MQ=255)
              aTCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATggagga                                                                                                                                    <  1:965462/150‑1 (MQ=255)
                 gTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAtt                                                                                                                                 >  2:4889546/1‑150 (MQ=255)
                 gTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAtt                                                                                                                                 <  1:512727/150‑1 (MQ=255)
                  ttCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAAttt                                                                                                                                >  2:750902/1‑150 (MQ=255)
                      ggAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCAtt                                                                                                                            >  2:2065835/1‑150 (MQ=255)
                        aaaCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTaa                                                                                                                          >  2:1740817/1‑150 (MQ=255)
                        aaaCGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTaa                                                                                                                          >  1:2154962/1‑150 (MQ=255)
                            gATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGACTTTCATTAACGGc                                                                                                                      >  1:3794142/1‑150 (MQ=255)
                             aTCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCg                                                                                                                     >  2:2993998/1‑150 (MQ=255)
                             aTCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCg                                                                                                                     <  1:1841089/150‑1 (MQ=255)
                              tCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGt                                                                                                                    >  1:1199817/1‑150 (MQ=255)
                              tCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGt                                                                                                                    >  1:901518/1‑150 (MQ=255)
                                aGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTAt                                                                                                                  >  1:2008580/1‑150 (MQ=255)
                                  gACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTa                                                                                                                <  1:287210/150‑1 (MQ=255)
                                    cAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTAcc                                                                                                              >  2:1663634/1‑150 (MQ=255)
                                       ggTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGAc                                                                                                           <  2:2514834/150‑1 (MQ=255)
                                         tGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGc                                                                                                         >  1:4301766/1‑150 (MQ=255)
                                           gCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTc                                                                                                       <  2:4017351/150‑1 (MQ=255)
                                           gCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTc                                                                                                       <  1:3627201/150‑1 (MQ=255)
                                           gCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTc                                                                                                       <  2:1439811/150‑1 (MQ=255)
                                            cGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCg                                                                                                      <  1:2556769/150‑1 (MQ=255)
                                            cGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCg                                                                                                      <  1:2419022/150‑1 (MQ=255)
                                            cGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCg                                                                                                      >  2:2043958/1‑150 (MQ=255)
                                              gggATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGcc                                                                                                    >  1:882464/1‑150 (MQ=255)
                                              gggATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGcc                                                                                                    >  1:1470402/1‑150 (MQ=255)
                                               ggATGATGATTTGGTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCGACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCa                                                                                                   <  2:953444/150‑1 (MQ=255)
                                                gatgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAg                                                                                                  <  1:688605/150‑1 (MQ=255)
                                                  tgatgatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGAGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGtt                                                                                                >  1:2366561/1‑150 (MQ=255)
                                                  tgatgatGTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAGGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGtt                                                                                                <  2:1779184/150‑1 (MQ=255)
                                                     tgatTTGTTGCGGTTCGCTGCGCCCGGGTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCa                                                                                             <  2:2334051/150‑1 (MQ=255)
                                                      gatTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCaa                                                                                            >  1:1224780/1‑150 (MQ=255)
                                                       atTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                           >  1:4122785/1‑150 (MQ=255)
                                                       atTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAg                                                                                           >  2:4246141/1‑150 (MQ=255)
                                                            ttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTc                                                                                      >  2:3340263/1‑150 (MQ=255)
                                                            ttgCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTc                                                                                      <  2:874213/150‑1 (MQ=255)
                                                             tgCGGTTCGCTGCGCCCGGGTACCGGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCg                                                                                     <  1:2275649/150‑1 (MQ=255)
                                                               cGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGAt                                                                                   <  1:775253/150‑1 (MQ=255)
                                                               cGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGAt                                                                                   >  2:1539875/1‑150 (MQ=255)
                                                                ggTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATg                                                                                  >  2:3735100/1‑150 (MQ=255)
                                                                 gTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGc                                                                                 <  1:4744910/150‑1 (MQ=255)
                                                                 gTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGc                                                                                 <  2:4687419/150‑1 (MQ=255)
                                                                   tCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGt                                                                               >  1:4694086/1‑150 (MQ=255)
                                                                   tCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGt                                                                               >  1:4946608/1‑150 (MQ=255)
                                                                     gCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTg                                                                             >  2:1946745/1‑150 (MQ=255)
                                                                     gCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTg                                                                             >  1:1577431/1‑150 (MQ=255)
                                                                      cTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGc                                                                            >  2:2146017/1‑150 (MQ=255)
                                                                         cgcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGctct                                                                         >  2:321148/1‑150 (MQ=255)
                                                                          gcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCtt                                                                        <  1:4378401/150‑1 (MQ=255)
                                                                          gcCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCtt                                                                        >  1:4294396/1‑150 (MQ=255)
                                                                                ttACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGt                                                                  >  1:3255499/1‑150 (MQ=255)
                                                                                    cTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTAttt                                                              <  1:1393902/150‑1 (MQ=255)
                                                                                       cgcgATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACt                                                           >  2:3949729/1‑150 (MQ=255)
                                                                                          gATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgc                                                        <  1:1534986/150‑1 (MQ=255)
                                                                                          gATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGAGGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTgc                                                        >  1:958027/1‑150 (MQ=255)
                                                                                                 tGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGt                                                 <  1:1702176/150‑1 (MQ=255)
                                                                                                  ggCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTg                                                <  2:1194247/150‑1 (MQ=255)
                                                                                                           ccTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACGTGCGCCAAGTGCCAGCgtgt                                       >  1:3387324/1‑150 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGTCAGCGTGTc                                      >  1:3018791/1‑150 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      <  2:2251606/150‑1 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      >  2:3691477/1‑150 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      <  2:584126/150‑1 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      >  2:1704095/1‑150 (MQ=255)
                                                                                                            cTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTc                                      <  2:1839874/150‑1 (MQ=255)
                                                                                                             tGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCg                                     >  2:2923971/1‑150 (MQ=255)
                                                                                                             tGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATGTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGATCTTGCAGGTATTTACGTGCGCCAAGTGCCAGCGTGTCg                                     >  2:5093300/1‑150 (MQ=255)
                                                                                                                cGTCCAGCGTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGACTTTCATTAACGGAGTATTACCGACGCGCGCCAGTTGCACGGGGTCGATGCGGTGCTCTTGCAGGTCTTTACTTGCGCCAAGTGCCAGCGAGTCgggt                                  >  2:2684305/1‑148 (MQ=255)
                                                                                                                 gTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTg                                 >  2:3054137/1‑150 (MQ=255)
                                                                                                                 gTCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTg                                 >  2:3128047/1‑150 (MQ=255)
                                                                                                                  tCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgc                                <  2:3085056/150‑1 (MQ=255)
                                                                                                                  tCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgc                                >  2:640745/1‑150 (MQ=255)
                                                                                                                  tCCAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgc                                <  1:1062053/150‑1 (MQ=255)
                                                                                                                    cAGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTgcgc                              >  2:2443532/1‑150 (MQ=255)
                                                                                                                     aGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGACTTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGca                             >  2:607876/1‑150 (MQ=255)
                                                                                                                     aGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGca                             <  1:2240741/150‑1 (MQ=255)
                                                                                                                     aGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGca                             >  1:1368179/1‑150 (MQ=255)
                                                                                                                     aGCTTCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGca                             >  2:4098096/1‑150 (MQ=255)
                                                                                                                        ttCGGCGTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAg                          <  1:2183870/150‑1 (MQ=255)
                                                                                                                              gTAACCGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGc                    >  2:3496171/1‑150 (MQ=255)
                                                                                                                                  ccGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTa                <  1:7951/150‑1 (MQ=255)
                                                                                                                                   cGGGATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAg               >  2:1598946/1‑150 (MQ=255)
                                                                                                                                    gggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGt              >  1:3338434/1‑150 (MQ=255)
                                                                                                                                    gggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGt              >  1:3989634/1‑150 (MQ=255)
                                                                                                                                     ggATCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGtt             <  2:3927725/150‑1 (MQ=255)
                                                                                                                                       aTCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTc           >  2:1561552/1‑150 (MQ=255)
                                                                                                                                       aTCTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTc           >  1:1629139/1‑150 (MQ=255)
                                                                                                                                         cTACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAg         <  2:2586905/150‑1 (MQ=255)
                                                                                                                                          tACGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAgg        <  2:4301953/150‑1 (MQ=255)
                                                                                                                                           aCGGTTACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGc       <  1:3172540/150‑1 (MQ=255)
                                                                                                                                                tACTATCTCCGGATGGAGGAATTTCATTAACGTCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGACGGTTGCGCACAGAAAGGCGGTAGTTTCAGGCGAAAt  >  1:1099563/1‑150 (MQ=255)
                                                                                                                                                tACTATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAt  <  2:4353792/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAGATCACAATTTATCGTTCAGGAAACGATCAGGACAGGGTGGCGGGGATGATGATTTGTTGCGGTTCGCTGCGCCCGGTTACCTGCGCGATCAACTGGCAAGCCGCCTGGCGTCCAGCTTCGGCGTAACCGGGATCTACGGTTACGATCTCCGGATGGAGGAATTTCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAAT  >  CP009273/4456086‑4456379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑