Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,465,936 T→A 100% L228* (TTA→TAA)  yjgL → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,465,9360TA97.7% 581.9 / 2.1 177L228* (TTA→TAA) yjgLuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (91/82);  total (92/85)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

AGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTTAT  >  CP009273/4465792‑4466082
                                                                                                                                                |                                                                                                                                                  
aGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATg                                                                                                                                               >  2:1164026/1‑150 (MQ=255)
 gAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGa                                                                                                                                              >  1:4661355/1‑150 (MQ=255)
 gAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGa                                                                                                                                              >  2:2768840/1‑150 (MQ=255)
     aTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATtct                                                                                                                                          >  1:2560785/1‑150 (MQ=255)
      tAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATtctc                                                                                                                                         >  2:1245818/1‑150 (MQ=255)
         cgcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAgg                                                                                                                                      >  1:1678811/1‑150 (MQ=255)
          gcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGa                                                                                                                                     <  1:1700728/150‑1 (MQ=255)
          gcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGa                                                                                                                                     >  2:1989247/1‑150 (MQ=255)
           cgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAg                                                                                                                                    <  2:4560807/150‑1 (MQ=255)
                       aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCg                                                                                                                        >  2:1144262/1‑150 (MQ=255)
                        ataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCg                                                                                                                        <  1:4118306/149‑1 (MQ=255)
                        ataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGc                                                                                                                       <  2:375920/150‑1 (MQ=255)
                         taatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCa                                                                                                                      <  1:3664743/150‑1 (MQ=255)
                          aatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAg                                                                                                                     >  1:3235123/1‑150 (MQ=255)
                          aatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAg                                                                                                                     >  2:1003587/1‑150 (MQ=255)
                          aatCAGGAGTTAAATAAGCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAg                                                                                                                     >  2:2145423/1‑150 (MQ=255)
                           atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAgg                                                                                                                    >  1:3298171/1‑150 (MQ=255)
                            tCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGa                                                                                                                   >  2:3125042/1‑150 (MQ=255)
                             cAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAATTAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGaa                                                                                                                  >  2:2104830/1‑150 (MQ=255)
                               ggAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAtt                                                                                                                >  2:4057293/1‑150 (MQ=255)
                               ggAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAtt                                                                                                                <  2:331935/150‑1 (MQ=255)
                                    tAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATa                                                                                                           <  2:486539/150‑1 (MQ=255)
                                      aataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt                                                                                                         >  1:2878802/1‑150 (MQ=255)
                                      aataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt                                                                                                         >  2:3085213/1‑150 (MQ=255)
                                      aataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt                                                                                                         >  2:2963079/1‑150 (MQ=255)
                                        taatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTcc                                                                                                       >  2:3489993/1‑150 (MQ=255)
                                           tCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca                                                                                                    <  2:4185766/150‑1 (MQ=255)
                                            cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg                                                                                                   >  1:2736148/1‑150 (MQ=255)
                                            cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg                                                                                                   <  2:2002631/150‑1 (MQ=255)
                                            cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg                                                                                                   <  1:4924050/150‑1 (MQ=255)
                                              acacacaTTAAATAATCTACAGACGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAACTAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGa                                                                                                 <  2:5064196/144‑1 (MQ=255)
                                                aGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGt                                                                                               <  1:3376249/150‑1 (MQ=255)
                                                  aaaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTa                                                                                             <  2:1743780/150‑1 (MQ=255)
                                                   aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTaa                                                                                            <  2:2906775/150‑1 (MQ=255)
                                                   aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTaa                                                                                            <  2:2906703/150‑1 (MQ=255)
                                                     ttAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAAt                                                                                          <  1:1013622/150‑1 (MQ=255)
                                                      tAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATg                                                                                         >  2:4412736/1‑150 (MQ=255)
                                                         ataatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                                                      >  1:3798749/1‑150 (MQ=255)
                                                          taatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATcc                                                                                     <  2:1460347/150‑1 (MQ=255)
                                                           aatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCg                                                                                    <  2:5088536/150‑1 (MQ=255)
                                                            atCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGc                                                                                   <  2:3810119/150‑1 (MQ=255)
                                                            atCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGATAAATAATCATCAGGAGATAAATAATCAGCAGAATTAAAATCATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATACTCCACAGCAGTTAAATGAtactc                                                                                   >  1:4943243/1‑146 (MQ=255)
                                                              cTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAg                                                                                 <  1:1528621/150‑1 (MQ=255)
                                                              cTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAg                                                                                 >  2:2607296/1‑150 (MQ=255)
                                                               tACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAgg                                                                                >  1:2411745/1‑150 (MQ=255)
                                                                aCAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGa                                                                               >  2:4077050/1‑150 (MQ=255)
                                                                   gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                                                            <  2:3056550/150‑1 (MQ=255)
                                                                   gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                                                            <  2:4779710/150‑1 (MQ=255)
                                                                   gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                                                            <  1:3483382/150‑1 (MQ=255)
                                                                     aGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTaa                                                                          <  2:3668412/150‑1 (MQ=255)
                                                                          aataatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAaataat                                                                     >  2:1686635/1‑150 (MQ=255)
                                                                              atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtctc                                                                 >  2:5009897/1‑150 (MQ=255)
                                                                              atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtctc                                                                 >  2:2367311/1‑150 (MQ=255)
                                                                                cTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAg                                                               >  1:329361/1‑150 (MQ=255)
                                                                                cTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAg                                                               >  2:2492503/1‑150 (MQ=255)
                                                                                 tACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAgg                                                              >  1:3642595/1‑150 (MQ=255)
                                                                                 tACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAgg                                                              <  1:151439/150‑1 (MQ=255)
                                                                                     gAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACtt                                                          <  1:2107704/150‑1 (MQ=255)
                                                                                      aaGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTa                                                         <  1:3355739/150‑1 (MQ=255)
                                                                                       aGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTaa                                                        <  2:2203660/150‑1 (MQ=255)
                                                                                       aGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTaa                                                        >  1:3754198/1‑150 (MQ=255)
                                                                                         ttAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAAt                                                      <  1:72018/150‑1 (MQ=255)
                                                                                              taataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAActc                                                 <  2:289991/150‑1 (MQ=255)
                                                                                               aataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAActct                                                <  2:406380/150‑1 (MQ=255)
                                                                                                atatACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                               <  2:3938014/150‑1 (MQ=255)
                                                                                                  ataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAg                                             >  2:2197193/1‑150 (MQ=255)
                                                                                                  ataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAg                                             >  1:321042/1‑150 (MQ=255)
                                                                                                    aCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGt                                           >  1:4144014/1‑150 (MQ=255)
                                                                                                     cAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTg                                          >  1:4693793/1‑150 (MQ=255)
                                                                                                       ggggTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAg                                        <  1:3052687/150‑1 (MQ=255)
                                                                                                        gggTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGt                                       >  1:122541/1‑150 (MQ=255)
                                                                                                         ggTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGtt                                      <  2:3741357/150‑1 (MQ=255)
                                                                                                          gTTAAATAATCCTCAGGAGTTAAATAGTCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTg                                     <  2:463883/150‑1 (MQ=255)
                                                                                                               ataatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAg                                >  1:2258834/1‑150 (MQ=255)
                                                                                                                 aatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGtt                              <  1:4578966/150‑1 (MQ=255)
                                                                                                                       cAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGtt                        <  2:761296/150‑1 (MQ=255)
                                                                                                                       cAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGtt                        <  1:4780935/150‑1 (MQ=255)
                                                                                                                        aGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTg                       >  2:107402/1‑150 (MQ=255)
                                                                                                                        aGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTg                       <  1:3954928/150‑1 (MQ=255)
                                                                                                                         ggAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGa                      >  2:627772/1‑150 (MQ=255)
                                                                                                                          gAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGAt                     >  1:3865216/1‑150 (MQ=255)
                                                                                                                           aGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGAtt                    >  1:3355291/1‑150 (MQ=255)
                                                                                                                            gTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTc                   <  1:4202494/150‑1 (MQ=255)
                                                                                                                              tAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTa                 <  2:399487/150‑1 (MQ=255)
                                                                                                                               aaaTAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTAc                <  1:1974887/150‑1 (MQ=255)
                                                                                                                                aataatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACg               <  1:2070799/150‑1 (MQ=255)
                                                                                                                                 ataatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGa              >  1:1090301/1‑150 (MQ=255)
                                                                                                                                   aatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAtt            >  2:3952901/1‑150 (MQ=255)
                                                                                                                                   aatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAtt            <  1:3856916/150‑1 (MQ=255)
                                                                                                                                   aatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAtt            >  2:1289665/1‑150 (MQ=255)
                                                                                                                                     tCCTCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCATGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTAc          <  1:4771883/150‑1 (MQ=255)
                                                                                                                                        gCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACggg       <  1:415434/150‑1 (MQ=255)
                                                                                                                                        gCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACggg       <  1:5009897/150‑1 (MQ=255)
                                                                                                                                        gCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACggg       <  2:1732759/150‑1 (MQ=255)
                                                                                                                                        gCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACggg       <  2:2986138/150‑1 (MQ=255)
                                                                                                                                           gAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGttt    <  1:3941992/150‑1 (MQ=255)
                                                                                                                                           gAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGttt    <  2:4495272/150‑1 (MQ=255)
                                                                                                                                            aaTTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTtta   >  1:3350051/1‑150 (MQ=255)
                                                                                                                                            aaTTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTtta   >  1:1383355/1‑150 (MQ=255)
                                                                                                                                             aTTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTttat  >  2:1001560/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTTAT  >  CP009273/4465792‑4466082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: