Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,480,139 G→T 100% A273E (GCG→GAG)  idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,480,1390GT97.0% 869.8 / 10.3 274A273E (GCG→GAG) idnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base T (139/126);  total (146/128)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

AGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGA  >  CP009273/4479992‑4480285
                                                                                                                                                   |                                                                                                                                                  
aGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCa                                                                                                                                                  >  1:4547611/1‑150 (MQ=255)
  gTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAAt                                                                                                                                                >  2:3407955/1‑150 (MQ=255)
     aaCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGa                                                                                                                                             >  1:3659052/1‑150 (MQ=255)
     aaCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGa                                                                                                                                             >  1:1528681/1‑150 (MQ=255)
     aaCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGa                                                                                                                                             >  1:2127747/1‑150 (MQ=255)
     aaCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGa                                                                                                                                             >  1:1559518/1‑150 (MQ=255)
       cAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                           >  1:2861609/1‑150 (MQ=255)
       cAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                           >  1:548368/1‑150 (MQ=255)
          tgttgtGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAAttt                                                                                                                                        <  2:1207569/150‑1 (MQ=255)
          tgttgtGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAAttt                                                                                                                                        <  1:2657297/150‑1 (MQ=255)
              gtggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                    >  2:35091/1‑150 (MQ=255)
                    gtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCAc                                                                                                                              <  2:2028058/150‑1 (MQ=255)
                     tgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACg                                                                                                                             >  1:1208847/1‑150 (MQ=255)
                     tgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACg                                                                                                                             <  2:625439/150‑1 (MQ=255)
                     tgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACg                                                                                                                             <  1:841438/150‑1 (MQ=255)
                      gATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACgg                                                                                                                            >  2:2549359/1‑150 (MQ=255)
                      gATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACgg                                                                                                                            <  2:2272958/150‑1 (MQ=255)
                        tCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCg                                                                                                                          <  2:762988/150‑1 (MQ=255)
                            tATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGt                                                                                                                      >  2:5107820/1‑150 (MQ=255)
                               ttttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTGCTGGCACGGCGAGGTTtc                                                                                                                   >  2:4721064/1‑150 (MQ=255)
                                  tAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgct                                                                                                                <  1:1132786/150‑1 (MQ=255)
                                   aaTTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctc                                                                                                               <  1:4868940/150‑1 (MQ=255)
                                   aaTTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctc                                                                                                               >  2:1112130/1‑150 (MQ=255)
                                    aTTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctcg                                                                                                              >  1:3808683/1‑150 (MQ=255)
                                    aTTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctcg                                                                                                              >  2:3190624/1‑150 (MQ=255)
                                      tttGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTcgcg                                                                                                            <  2:5150436/150‑1 (MQ=255)
                                       ttGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCgg                                                                                                           >  1:4746178/1‑150 (MQ=255)
                                        tGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGc                                                                                                          >  1:1401044/1‑150 (MQ=255)
                                           tCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcaca                                                                                                       <  2:3763789/150‑1 (MQ=255)
                                           tCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcaca                                                                                                       >  2:3689152/1‑150 (MQ=255)
                                           tCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcaca                                                                                                       >  1:3011644/1‑150 (MQ=255)
                                                aaCATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGa                                                                                                  >  1:2320375/1‑150 (MQ=255)
                                                 aCATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGaa                                                                                                 >  2:2964652/1‑150 (MQ=255)
                                                      tGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCa                                                                                            <  1:4573255/150‑1 (MQ=255)
                                                      tGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCa                                                                                            <  1:796242/150‑1 (MQ=255)
                                                         gcggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCAccc                                                                                         >  2:1892145/1‑150 (MQ=255)
                                                          cggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCa                                                                                        >  1:4527027/1‑150 (MQ=255)
                                                          cggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCa                                                                                        <  1:2907297/150‑1 (MQ=255)
                                                           ggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCAt                                                                                       >  2:393077/1‑150 (MQ=255)
                                                            gcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATc                                                                                      >  2:595311/1‑150 (MQ=255)
                                                            gcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATc                                                                                      <  2:1838495/150‑1 (MQ=255)
                                                             ccATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCg                                                                                     <  1:4278654/150‑1 (MQ=255)
                                                             ccATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCg                                                                                     <  2:2644530/150‑1 (MQ=255)
                                                             ccATCCGACCAATATCAAAACGCGGAGTAATTACGCTGGCGAGGCTTTGGATCAATTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGTCGAGGTTTCGCTCTCGGCACAGCAGAAGCGCACCCATCg                                                                                     <  2:3025128/150‑1 (MQ=255)
                                                              cATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGc                                                                                    >  1:2539179/1‑150 (MQ=255)
                                                              cATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGc                                                                                    >  2:5004486/1‑150 (MQ=255)
                                                              cATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGc                                                                                    <  2:4540066/150‑1 (MQ=255)
                                                                 ccGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAat                                                                                 >  2:1294165/1‑150 (MQ=255)
                                                                  cGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAata                                                                                >  2:2493136/1‑150 (MQ=255)
                                                                   gACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAatat                                                                               <  2:1146437/150‑1 (MQ=255)
                                                                     ccAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCTGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCg                                                                             <  2:3425064/150‑1 (MQ=255)
                                                                      cAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGt                                                                            >  2:712934/1‑150 (MQ=255)
                                                                         tatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAt                                                                         <  1:2420724/150‑1 (MQ=255)
                                                                            cAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTgg                                                                      >  2:2217060/1‑150 (MQ=255)
                                                                              aaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTGTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTg                                                                    >  1:910558/1‑150 (MQ=255)
                                                                               aaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGc                                                                   >  1:1093239/1‑150 (MQ=255)
                                                                                 cGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCaa                                                                 >  2:1380160/1‑150 (MQ=255)
                                                                                  ggggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCaaa                                                                >  1:3262433/1‑150 (MQ=255)
                                                                                     gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATa                                                             <  2:4513665/150‑1 (MQ=255)
                                                                                        tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATacac                                                          >  1:5075637/1‑150 (MQ=255)
                                                                                         aaTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACAcc                                                         <  1:1732056/150‑1 (MQ=255)
                                                                                            tACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTc                                                      <  1:3977626/150‑1 (MQ=255)
                                                                                             aCGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCt                                                     <  2:2507442/150‑1 (MQ=255)
                                                                                                  ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                >  2:5032157/1‑150 (MQ=255)
                                                                                                   gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCGCCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATcc                                               <  2:854695/150‑1 (MQ=255)
                                                                                                     gAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCgg                                             >  2:1474918/1‑150 (MQ=255)
                                                                                                     gAGGCTTGGGATCATTGGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCgg                                             <  1:34985/150‑1 (MQ=255)
                                                                                                      aGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCggg                                            <  2:3921748/150‑1 (MQ=255)
                                                                                                        gCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGtt                                          <  1:2999476/150‑1 (MQ=255)
                                                                                                          ttGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTg                                        >  2:288976/1‑150 (MQ=255)
                                                                                                          ttGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTg                                        <  1:3587735/150‑1 (MQ=255)
                                                                                                           tGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgc                                       <  2:926046/150‑1 (MQ=255)
                                                                                                                 cATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAg                                 <  2:2692891/150‑1 (MQ=255)
                                                                                                                  aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAgg                                <  2:4352148/150‑1 (MQ=255)
                                                                                                                   tttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAggg                               >  2:4146898/1‑150 (MQ=255)
                                                                                                                   tttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAggg                               <  2:3139538/150‑1 (MQ=255)
                                                                                                                    ttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGc                              >  2:815653/1‑150 (MQ=255)
                                                                                                                       cctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAta                           <  1:4014698/150‑2 (MQ=255)
                                                                                                                       cctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATc                           <  1:2187704/150‑1 (MQ=255)
                                                                                                                            cAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                      >  1:1810084/1‑150 (MQ=255)
                                                                                                                            cAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                      >  2:4789047/1‑150 (MQ=255)
                                                                                                                              aTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAtt                    >  2:2932226/1‑150 (MQ=255)
                                                                                                                                 tcCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc                 >  1:1895360/1‑150 (MQ=255)
                                                                                                                                  ccAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGGGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACt                <  1:2702290/150‑1 (MQ=255)
                                                                                                                                      cccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt            <  2:2350499/150‑1 (MQ=255)
                                                                                                                                       ccATGAAAACCCTCAATGGAAATTTGTTCTGGCACTGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAtt           <  2:464271/150‑1 (MQ=255)
                                                                                                                                       ccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAtt           <  1:4146898/150‑1 (MQ=255)
                                                                                                                                        cATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTc          <  1:4058153/150‑1 (MQ=255)
                                                                                                                                        cATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTc          <  2:283717/150‑1 (MQ=255)
                                                                                                                                         aTGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc         >  1:3288896/1‑150 (MQ=255)
                                                                                                                                          tGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt        <  2:492590/150‑1 (MQ=255)
                                                                                                                                          tGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt        >  2:652484/1‑150 (MQ=255)
                                                                                                                                           gAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCTGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa       <  1:3407955/150‑1 (MQ=255)
                                                                                                                                           gAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa       <  2:1719590/150‑1 (MQ=255)
                                                                                                                                           gAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa       <  1:3407988/150‑1 (MQ=255)
                                                                                                                                             aaaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTaaa     <  2:4862253/150‑1 (MQ=255)
                                                                                                                                              aaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaat    >  1:4991612/1‑150 (MQ=255)
                                                                                                                                               aCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatg   >  1:1163502/1‑150 (MQ=255)
                                                                                                                                                cccTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCTCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCATAaatga  <  1:637115/150‑1 (MQ=255)
                                                                                                                                                cccTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatga  >  2:840122/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGA  >  CP009273/4479992‑4480285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑