Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,547,818 C→A 100% D97Y (GAT→TAT)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,547,8180CA96.7% 758.2 / 11.7 242D97Y (GAT→TAT) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base A (137/97);  total (140/102)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

CTTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCG  >  CP009273/4547671‑4547964
                                                                                                                                                   |                                                                                                                                                  
cTTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGc                                                                                                                                                  >  1:1626692/1‑150 (MQ=255)
  tCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTg                                                                                                                                                >  2:1906326/1‑150 (MQ=255)
        aGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCa                                                                                                                                          >  1:948559/1‑150 (MQ=255)
         gTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCAc                                                                                                                                         <  2:4594053/150‑1 (MQ=255)
           ttATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGc                                                                                                                                       >  1:3633891/1‑150 (MQ=255)
            tATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCa                                                                                                                                      >  1:1447112/1‑150 (MQ=255)
            tATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGCAATAGCTGCCGCCACGCa                                                                                                                                      >  2:259580/1‑150 (MQ=255)
                 aCCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGGAAAGAGCGGCGGCATCTGGCTGATCTTGGACGGTAACAGCCCGAGGGACGGTGTGTGTGCAGCGGGTATCTTGATGTGTCGTTGCGCGTACTAGATGCCGCCACGCCGctct                                                                                                                                 >  2:1950212/1‑150 (MQ=255)
                  ccGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTg                                                                                                                                >  2:2417030/1‑150 (MQ=255)
                    gCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGcc                                                                                                                              <  2:1244231/150‑1 (MQ=255)
                    gCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGcc                                                                                                                              <  2:3222498/150‑1 (MQ=255)
                    gCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGcc                                                                                                                              >  1:3207107/1‑150 (MQ=255)
                    gCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGGAATAGCTGCCGCCACGCAGCTCTGcc                                                                                                                              >  1:4667704/1‑150 (MQ=255)
                      ttCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAg                                                                                                                            >  1:4476009/1‑150 (MQ=255)
                        cATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGAt                                                                                                                          >  2:1695706/1‑150 (MQ=255)
                          tCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTg                                                                                                                        >  1:3833597/1‑150 (MQ=255)
                               aTCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctc                                                                                                                   >  2:4957119/1‑150 (MQ=255)
                                 cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTa                                                                                                                 <  2:2449350/150‑1 (MQ=255)
                                   aTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAAc                                                                                                               <  2:3088202/150‑1 (MQ=255)
                                    tAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACt                                                                                                              >  2:2542627/1‑150 (MQ=255)
                                    tAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACt                                                                                                              >  2:901667/1‑150 (MQ=255)
                                     aGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTg                                                                                                             >  1:1336912/1‑150 (MQ=255)
                                      gCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGt                                                                                                            <  1:1125827/150‑1 (MQ=255)
                                        ttCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTCTCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTg                                                                                                          >  1:647488/1‑150 (MQ=255)
                                        ttCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTg                                                                                                          >  1:647311/1‑150 (MQ=255)
                                            aTTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAAc                                                                                                      >  1:4858945/1‑150 (MQ=255)
                                             ttGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACa                                                                                                     <  1:311431/150‑1 (MQ=255)
                                              tGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAg                                                                                                    <  2:4983830/150‑1 (MQ=255)
                                                 cnAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACGGTTGCAACAGATg                                                                                                 >  1:2503996/1‑150 (MQ=255)
                                                 ccAGGTACAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATg                                                                                                 <  1:125344/150‑1 (MQ=255)
                                                 ccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATg                                                                                                 >  2:4261671/1‑150 (MQ=255)
                                                 ccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATg                                                                                                 >  2:3502174/1‑150 (MQ=255)
                                                 ccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATg                                                                                                 <  2:3627764/150‑1 (MQ=255)
                                                  cAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATgg                                                                                                >  1:4778741/1‑150 (MQ=255)
                                                    ggTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCg                                                                                              <  1:1980422/150‑1 (MQ=255)
                                                      tAAAGAGCGGCGGCATCTGTCTGATAATGGACTGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATATTTCGTTGCGCGAAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                            >  1:4076909/1‑150 (MQ=255)
                                                      tAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                            >  2:4868330/1‑150 (MQ=255)
                                                        aaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATc                                                                                          <  2:792729/150‑1 (MQ=255)
                                                        aaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATc                                                                                          <  1:4980254/150‑1 (MQ=255)
                                                         agagCGGCGTCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCt                                                                                         <  2:274583/150‑1 (MQ=255)
                                                           agCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGc                                                                                       <  2:4321035/150‑1 (MQ=255)
                                                              ggcggcATCTGGCTGATCCTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGGAATAGCTGCCGCCACGCAGCTCTGCCCGAGTGCTCTCTAACTGTTGCACCAGATGGCGGAGCTGCGaa                                                                                    >  2:3116690/1‑150 (MQ=255)
                                                              ggcggcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGaa                                                                                    >  2:5078297/1‑150 (MQ=255)
                                                                    aTCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAg                                                                              <  1:3366453/150‑1 (MQ=255)
                                                                       tGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtt                                                                           >  2:1980760/1‑150 (MQ=255)
                                                                         gCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtttt                                                                         <  2:2903473/150‑1 (MQ=255)
                                                                            gATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTccc                                                                      >  1:3930712/1‑150 (MQ=255)
                                                                              tAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCtt                                                                    <  1:1906326/150‑1 (MQ=255)
                                                                               aaTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                   <  1:55181/150‑1 (MQ=255)
                                                                               aaTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                   >  2:3393988/1‑150 (MQ=255)
                                                                               aaTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                   <  2:1627049/150‑1 (MQ=255)
                                                                                 tGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGtt                                                                 >  2:2872610/1‑150 (MQ=255)
                                                                                 tGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGtt                                                                 >  2:2135432/1‑150 (MQ=255)
                                                                                   gACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCt                                                               <  2:353091/150‑1 (MQ=255)
                                                                                     cGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTga                                                             <  1:1615883/150‑1 (MQ=255)
                                                                                        tAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                          >  2:4879670/1‑150 (MQ=255)
                                                                                        tAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                          >  2:4000474/1‑150 (MQ=255)
                                                                                           cAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTg                                                       <  1:2878125/150‑1 (MQ=255)
                                                                                            aGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCCGAGAGTTGc                                                      >  1:1062042/1‑150 (MQ=255)
                                                                                                   gggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcggcg                                               >  1:5068727/1‑150 (MQ=255)
                                                                                                    ggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGt                                              <  1:1020479/150‑1 (MQ=255)
                                                                                                     gAAAGAGAGTGTGTAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGtt                                             >  1:2417975/1‑150 (MQ=255)
                                                                                                      aaaGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTa                                            >  1:5136869/1‑150 (MQ=255)
                                                                                                      aaaGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTa                                            >  1:228580/1‑150 (MQ=255)
                                                                                                       aaGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTAc                                           >  2:1225419/1‑150 (MQ=255)
                                                                                                        agagagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTAcc                                          >  1:386872/1‑150 (MQ=255)
                                                                                                           gagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                       >  1:1004614/1‑150 (MQ=255)
                                                                                                           gagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                       <  1:1354984/150‑1 (MQ=255)
                                                                                                           gagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATATTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                       >  1:2523734/1‑150 (MQ=255)
                                                                                                            agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGc                                      >  2:4064371/1‑150 (MQ=255)
                                                                                                             gtgtGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCg                                     >  2:741307/1‑150 (MQ=255)
                                                                                                             gtgtGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCg                                     <  2:1148131/150‑1 (MQ=255)
                                                                                                               gtgCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGt                                   <  2:541661/150‑1 (MQ=255)
                                                                                                                 gCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTa                                 <  1:4267447/150‑1 (MQ=255)
                                                                                                                  cAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTaa                                <  1:1695706/150‑1 (MQ=255)
                                                                                                                    gCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAAc                              <  1:141321/150‑1 (MQ=255)
                                                                                                                        cTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAtt                          >  2:4515951/1‑150 (MQ=255)
                                                                                                                        cTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAtt                          <  1:3134154/150‑1 (MQ=255)
                                                                                                                        cTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGAACTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAtt                          >  1:349170/1‑150 (MQ=255)
                                                                                                                         tATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTc                         >  1:2518401/1‑150 (MQ=255)
                                                                                                                         tATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTc                         >  1:2637970/1‑150 (MQ=255)
                                                                                                                          aTCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                        <  2:3886458/150‑1 (MQ=255)
                                                                                                                          aTCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                        <  2:2318479/150‑1 (MQ=255)
                                                                                                                          aTCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                        >  2:3449566/1‑150 (MQ=255)
                                                                                                                          aTCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCa                        <  1:1875674/150‑1 (MQ=255)
                                                                                                                              tGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAacac                    <  1:901667/150‑1 (MQ=255)
                                                                                                                               gATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACAcc                   >  1:2926133/1‑150 (MQ=255)
                                                                                                                                aTTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCa                  <  2:1403204/150‑1 (MQ=255)
                                                                                                                                 tttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCaa                 <  1:3250764/150‑1 (MQ=255)
                                                                                                                                   tttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATc               >  1:2257622/1‑150 (MQ=255)
                                                                                                                                    ttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCg              >  1:4593171/1‑150 (MQ=255)
                                                                                                                                     tCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTATACAATTCAACACCAATCGc             >  2:4412569/1‑150 (MQ=255)
                                                                                                                                      cGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGcc            <  2:3798006/150‑1 (MQ=255)
                                                                                                                                      cGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGcc            >  2:4745732/1‑150 (MQ=255)
                                                                                                                                       gTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCt           <  2:4134257/150‑1 (MQ=255)
                                                                                                                                       gTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCt           >  2:2963509/1‑150 (MQ=255)
                                                                                                                                           cgcgTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTc       >  1:4059641/1‑150 (MQ=255)
                                                                                                                                             cgTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCa     <  2:4476009/150‑1 (MQ=255)
                                                                                                                                              gTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAg    >  2:1506352/1‑150 (MQ=255)
                                                                                                                                                aaTAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCATTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAgcg  >  2:1727784/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCG  >  CP009273/4547671‑4547964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑