Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,548,084 A→T 100% L8* (TTG→TAG)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,548,0840AT96.3% 958.5 / 17.9 296L8* (TTG→TAG) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base T (141/144);  total (147/149)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 297 total aligned reads displayed.

TCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGCCGCG  >  CP009273/4547940‑4548233
                                                                                                                                                |                                                                                                                                                     
tCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATc                                                                                                                                                  <  1:1043102/150‑1 (MQ=255)
 cAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCg                                                                                                                                                 >  1:971609/1‑150 (MQ=255)
   acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGca                                                                                                                                               >  2:3498961/1‑150 (MQ=255)
       cAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcaccac                                                                                                                                           <  2:805422/150‑1 (MQ=255)
       cAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcaccac                                                                                                                                           >  2:2033074/1‑150 (MQ=255)
        aaTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACcaca                                                                                                                                          >  2:458001/1‑150 (MQ=255)
          tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGt                                                                                                                                        <  2:1539717/150‑1 (MQ=255)
           cGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTc                                                                                                                                       >  1:1312159/1‑150 (MQ=255)
             ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCAt                                                                                                                                     <  2:228580/150‑1 (MQ=255)
             ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCAt                                                                                                                                     >  2:3968786/1‑150 (MQ=255)
              cTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATc                                                                                                                                    >  2:2901301/1‑150 (MQ=255)
               tGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATcc                                                                                                                                   <  1:4261671/150‑1 (MQ=255)
                gTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCa                                                                                                                                  <  1:1225419/150‑1 (MQ=255)
                   ccAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTa                                                                                                                               <  2:647311/150‑1 (MQ=255)
                   ccAGCGCACGGATGCGGCGGCCGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTa                                                                                                                               <  2:3485580/150‑1 (MQ=255)
                     aGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACg                                                                                                                             <  1:741307/150‑1 (MQ=255)
                       cgcACGGATGTGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGtt                                                                                                                           >  2:1237885/1‑150 (MQ=255)
                           cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTGTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTAc                                                                                                                       >  2:4418533/1‑150 (MQ=255)
                             gATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTActc                                                                                                                     >  2:4021997/1‑150 (MQ=255)
                              aTGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTActct                                                                                                                    >  1:2997623/1‑150 (MQ=255)
                              aTGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGATAAATCGCACCACAGTCATCCATTACGTTTTActct                                                                                                                    >  2:4143916/1‑150 (MQ=255)
                                gcggcgGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGt                                                                                                                  <  2:3737651/150‑1 (MQ=255)
                                    cggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgc                                                                                                              <  1:4064371/150‑1 (MQ=255)
                                      gcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccg                                                                                                            <  1:4649190/150‑1 (MQ=255)
                                       cTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgc                                                                                                           >  1:4430635/1‑150 (MQ=255)
                                        tGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                          >  1:4863245/1‑150 (MQ=255)
                                         gAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                         >  1:2601047/1‑150 (MQ=255)
                                         gAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                         <  2:1345199/150‑1 (MQ=255)
                                           aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCtt                                                                                                       <  1:4745732/150‑1 (MQ=255)
                                           aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCtt                                                                                                       <  1:5154510/150‑1 (MQ=255)
                                           aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTGTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCtt                                                                                                       <  2:4352915/150‑1 (MQ=255)
                                             gCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGc                                                                                                     >  1:1476535/1‑150 (MQ=255)
                                                   tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAt                                                                                               >  1:1018289/1‑150 (MQ=255)
                                                   tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAt                                                                                               >  1:3329834/1‑150 (MQ=255)
                                                     cGAGACGTTGCGACTGACTGCCGCCTGGGAAATATTTCGGCATTTTTCTAGGGTCAGAAAATCTTAAAGACATTTGGTTTCTTTATTTTGCTAAATCGCACAACTTTCATCCATTACGTTTTACTCTGTCATCCTCCCTTTCTGGCAttg                                                                                             <  2:1909559/150‑2 (MQ=255)
                                                     cGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTc                                                                                             <  1:3851379/150‑1 (MQ=255)
                                                     cGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTc                                                                                             >  2:4619780/1‑150 (MQ=255)
                                                      gagaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCt                                                                                            <  1:790350/150‑1 (MQ=255)
                                                       agaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                                                                           <  2:5139637/150‑1 (MQ=255)
                                                         aCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACg                                                                                         <  2:2503996/150‑1 (MQ=255)
                                                         aCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACg                                                                                         <  1:3042287/150‑1 (MQ=255)
                                                             tGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCa                                                                                     <  1:325351/150‑1 (MQ=255)
                                                                 actgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCg                                                                                 <  1:2892267/150‑1 (MQ=255)
                                                                  ctgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCgg                                                                                <  2:2545667/150‑1 (MQ=255)
                                                                   tgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCggg                                                                               <  1:2954330/150‑1 (MQ=255)
                                                                   tgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCggg                                                                               <  1:3449566/150‑1 (MQ=255)
                                                                     actgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcg                                                                             <  1:3063698/150‑1 (MQ=255)
                                                                     actgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcg                                                                             <  2:1983890/150‑1 (MQ=255)
                                                                        gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCt                                                                          <  1:2664041/150‑1 (MQ=255)
                                                                          cgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGa                                                                        <  1:1950151/150‑1 (MQ=255)
                                                                            ccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACa                                                                      <  1:1006835/150‑1 (MQ=255)
                                                                            ccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACa                                                                      <  1:1530355/150‑1 (MQ=255)
                                                                             cTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                     <  1:458001/150‑1 (MQ=255)
                                                                             cTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                     <  1:3926508/150‑1 (MQ=255)
                                                                               gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAcc                                                                   <  1:3803585/150‑1 (MQ=255)
                                                                               gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAcc                                                                   <  1:4515951/150‑1 (MQ=255)
                                                                               gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAcc                                                                   >  2:2936259/1‑150 (MQ=255)
                                                                                   aaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGTGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGttt                                                               >  1:244553/1‑150 (MQ=255)
                                                                                    aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTa                                                              >  2:1036354/1‑150 (MQ=255)
                                                                                    aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTa                                                              <  2:4954368/150‑1 (MQ=255)
                                                                                      aTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAg                                                            >  2:937470/1‑150 (MQ=255)
                                                                                        tGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGcc                                                          <  1:4412569/150‑1 (MQ=255)
                                                                                        tGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGcc                                                          <  1:696840/150‑1 (MQ=255)
                                                                                         gCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCg                                                         >  2:3108300/1‑150 (MQ=255)
                                                                                           ggCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                       >  1:3526274/1‑150 (MQ=255)
                                                                                           ggCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                       >  2:3229334/1‑150 (MQ=255)
                                                                                            gCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                      >  1:2076864/1‑150 (MQ=255)
                                                                                             cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTc                                                     >  1:1142902/1‑150 (MQ=255)
                                                                                              aTTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCa                                                    <  1:2159448/150‑1 (MQ=255)
                                                                                              aTTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCa                                                    >  1:3173245/1‑150 (MQ=255)
                                                                                                ttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCaaa                                                  >  1:2223716/1‑150 (MQ=255)
                                                                                                 tttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAAc                                                 <  2:263775/150‑1 (MQ=255)
                                                                                                       gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCt                                           >  1:2324965/1‑150 (MQ=255)
                                                                                                       gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCt                                           <  2:1312159/150‑1 (MQ=255)
                                                                                                        ggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCtt                                          <  2:1762979/150‑1 (MQ=255)
                                                                                                        ggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCtt                                          >  2:4713561/1‑150 (MQ=255)
                                                                                                            aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                      <  1:1685953/150‑1 (MQ=255)
                                                                                                            aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                      >  2:503676/1‑150 (MQ=255)
                                                                                                            aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                      <  1:908267/150‑1 (MQ=255)
                                                                                                            aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                      >  2:666402/1‑150 (MQ=255)
                                                                                                                 aTCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAgg                                 <  2:2042208/150‑1 (MQ=255)
                                                                                                                 aTCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAgg                                 <  2:4817253/150‑1 (MQ=255)
                                                                                                                   cATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCt                               >  2:1299249/1‑150 (MQ=255)
                                                                                                                   cATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCt                               >  2:1664195/1‑150 (MQ=255)
                                                                                                                     tAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCttt                             <  1:4377053/150‑1 (MQ=255)
                                                                                                                     tAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCttt                             <  1:1138191/150‑1 (MQ=255)
                                                                                                                      aaaGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTg                            >  1:2545727/1‑150 (MQ=255)
                                                                                                                       aaGCCATTTGGTTTCAATATTATTATAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGc                           >  2:1180073/1‑150 (MQ=255)
                                                                                                                        aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                      >  1:2840310/1‑122 (MQ=255)
                                                                                                                        aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                      >  1:3427593/1‑122 (MQ=255)
                                                                                                                        aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGttt                                                      <  2:2840310/122‑1 (MQ=255)
                                                                                                                        aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                          <  2:3332512/150‑1 (MQ=255)
                                                                                                                        aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                          <  2:748025/150‑1 (MQ=255)
                                                                                                                          ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGt                        >  1:842321/1‑150 (MQ=255)
                                                                                                                               tGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTg                   <  2:2451809/150‑1 (MQ=255)
                                                                                                                               tGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTg                   >  1:2003070/1‑150 (MQ=255)
                                                                                                                               tGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTg                   >  1:941518/1‑150 (MQ=255)
                                                                                                                                ggTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGa                  >  2:1701619/1‑150 (MQ=255)
                                                                                                                                          ttatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGttt        <  1:3642161/150‑1 (MQ=255)
                                                                                                                                            atGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGc      >  2:3801025/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGCCGCG  >  CP009273/4547940‑4548233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑