Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,567,278 G→T 100% I166I (ATC→ATA)  mcrC ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,567,2780GT97.5% 618.6 / 4.3 197I166I (ATC→ATA) mcrC5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (91/101);  total (93/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 200 total aligned reads displayed.

AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGC  >  CP009273/4567131‑4567422
                                                                                                                                                   |                                                                                                                                                
aGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTAt                                                                                                                                                <  2:105123/150‑1 (MQ=255)
 gACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTtata                                                                                                                                               >  2:4693925/1‑150 (MQ=255)
 gACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTtata                                                                                                                                               <  1:4232723/150‑1 (MQ=255)
   cGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTTTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAAc                                                                                                                                             <  1:3568362/150‑1 (MQ=255)
           cACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTGTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTtat                                                                                                                                     >  2:3045729/1‑150 (MQ=255)
            acgacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTtata                                                                                                                                    <  1:4031326/150‑1 (MQ=255)
               acgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataata                                                                                                                                 >  2:1342925/1‑150 (MQ=255)
               acgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataata                                                                                                                                 >  2:1525701/1‑150 (MQ=255)
                cgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTAtaataa                                                                                                                                >  1:4607274/1‑150 (MQ=255)
                    aaaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAcgcg                                                                                                                            >  1:1096975/1‑150 (MQ=255)
                     aaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGt                                                                                                                           <  1:1030743/150‑1 (MQ=255)
                     aaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGt                                                                                                                           >  1:4549668/1‑150 (MQ=255)
                      aaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTa                                                                                                                          >  2:2820136/1‑150 (MQ=255)
                        ttCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAtt                                                                                                                        <  2:3313523/150‑1 (MQ=255)
                        ttCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAtt                                                                                                                        <  1:3375046/150‑1 (MQ=255)
                           aTAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAttttt                                                                                                                     >  2:3377541/1‑150 (MQ=255)
                            tAAAGAAACATTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAtttttt                                                                                                                    <  1:2944894/150‑1 (MQ=255)
                             aaaGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTc                                                                                                                   <  1:1544906/150‑1 (MQ=255)
                              aaGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTcc                                                                                                                  >  1:2751041/1‑150 (MQ=255)
                               aGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCg                                                                                                                 >  2:329335/1‑150 (MQ=255)
                                  aaCTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCa                                                                                                              >  2:3087071/1‑150 (MQ=255)
                                  aaCTTTTGATAAAGTAATGACATCTATTTTTCTTTTATTTCTAAATCATAGAAACTGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTTCAGAAACTTATAACGAATTTATAATAACGCGTATTTTTTcctaca                                                                                                              <  2:249410/150‑5 (MQ=255)
                                   aCTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCAt                                                                                                             <  1:847851/150‑1 (MQ=255)
                                      tttGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCa                                                                                                          <  1:306676/150‑1 (MQ=255)
                                       ttGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAg                                                                                                         <  2:2560891/150‑1 (MQ=255)
                                          aTAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATa                                                                                                      <  1:1193653/150‑1 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTg                                                                                                  >  1:1470680/1‑150 (MQ=255)
                                               gTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGa                                                                                                 <  1:1584386/150‑1 (MQ=255)
                                                 aaTTACATCTATTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCATTTGTTTTGACCTGGAATATAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaaa                                                                                               <  2:4320596/150‑1 (MQ=255)
                                                   tGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtg                                                                                             <  1:4182073/150‑1 (MQ=255)
                                                     aCATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgct                                                                                           >  2:3172415/1‑150 (MQ=255)
                                                       aTCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctgc                                                                                         >  1:747134/1‑150 (MQ=255)
                                                       aTCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctgc                                                                                         >  2:2333582/1‑150 (MQ=255)
                                                        tctcTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCg                                                                                        <  2:2363548/150‑1 (MQ=255)
                                                         ctctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCgg                                                                                       <  2:494758/150‑1 (MQ=255)
                                                            tttttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGt                                                                                    <  2:3709964/150‑1 (MQ=255)
                                                             ttttCGTTTCTTTCAAAATCCTAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGtt                                                                                   <  2:3053391/150‑1 (MQ=255)
                                                             ttttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGtt                                                                                   <  1:3969727/150‑1 (MQ=255)
                                                             ttttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGtt                                                                                   <  2:393652/150‑1 (MQ=255)
                                                             ttttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTAGACCAGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAATTATAATAACGAGTATTTTTTCCGCCATTCAGAAAGCTGAAATGCTGCGGAGtt                                                                                   >  1:1702321/1‑150 (MQ=255)
                                                              tttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTa                                                                                  >  2:1976802/1‑150 (MQ=255)
                                                               ttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaa                                                                                 >  1:2383925/1‑150 (MQ=255)
                                                               ttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaa                                                                                 >  2:125948/1‑150 (MQ=255)
                                                                 cGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAAt                                                                               >  2:4811356/1‑150 (MQ=255)
                                                                  gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                                                              <  1:5011895/150‑1 (MQ=255)
                                                                     tctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAag                                                                           >  1:2538524/1‑150 (MQ=255)
                                                                       tttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAagag                                                                         <  2:1183613/150‑1 (MQ=255)
                                                                         tcAAAATCAGAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTg                                                                       <  2:5062592/150‑1 (MQ=255)
                                                                          cAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGc                                                                      <  2:3273682/150‑1 (MQ=255)
                                                                           aaaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCt                                                                     >  2:794069/1‑150 (MQ=255)
                                                                            aaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTa                                                                    >  2:1159377/1‑150 (MQ=255)
                                                                            aaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTa                                                                    <  1:1423449/150‑1 (MQ=255)
                                                                             aaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTaa                                                                   >  1:1250561/1‑150 (MQ=255)
                                                                               tCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATg                                                                 >  2:3344383/1‑150 (MQ=255)
                                                                                cATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGATGCGGAGTTAAATTAAGAGTGCTAATGc                                                                >  2:4404733/1‑150 (MQ=255)
                                                                                  tAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGccc                                                              <  1:3308661/150‑1 (MQ=255)
                                                                                   aGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGccca                                                             <  2:3698896/150‑2 (MQ=255)
                                                                                   aGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCg                                                             <  2:2731057/150‑1 (MQ=255)
                                                                                   aGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCg                                                             >  1:4812453/1‑150 (MQ=255)
                                                                                    gAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTACGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCgg                                                            >  1:486048/1‑150 (MQ=255)
                                                                                     aaaCGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGt                                                           >  1:2290883/1‑150 (MQ=255)
                                                                                       aCGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTCATGCCCGGTaa                                                         >  2:3483796/1‑150 (MQ=255)
                                                                                         ggTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAAtt                                                       <  1:3610215/150‑1 (MQ=255)
                                                                                         ggTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAAtt                                                       >  2:4476396/1‑150 (MQ=255)
                                                                                              tgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCta                                                  >  2:2177449/1‑150 (MQ=255)
                                                                                              tgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCta                                                  >  1:2997939/1‑150 (MQ=255)
                                                                                               gtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCtat                                                 >  2:3412499/1‑150 (MQ=255)
                                                                                                 ccTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATaa                                               >  2:619499/1‑150 (MQ=255)
                                                                                                   tttGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAg                                             >  1:1826637/1‑150 (MQ=255)
                                                                                                          tGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGa                                      >  1:3688367/1‑150 (MQ=255)
                                                                                                           gACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAg                                     >  2:4146887/1‑150 (MQ=255)
                                                                                                             ccTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCt                                   >  1:1366918/1‑150 (MQ=255)
                                                                                                              cTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt                                  <  2:346570/150‑1 (MQ=255)
                                                                                                               tGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTc                                 >  2:2770891/1‑150 (MQ=255)
                                                                                                                 gAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAt                               <  1:1107093/150‑1 (MQ=255)
                                                                                                                  aaTAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAtc                              <  1:4404727/150‑1 (MQ=255)
                                                                                                                    tAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAtctc                            >  1:4166676/1‑150 (MQ=255)
                                                                                                                      gAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTg                          >  2:2203013/1‑150 (MQ=255)
                                                                                                                       aaTTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGa                         <  1:3377663/150‑1 (MQ=255)
                                                                                                                       aaTTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGa                         <  1:4299637/150‑1 (MQ=255)
                                                                                                                        attattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGAt                        <  2:1951620/150‑1 (MQ=255)
                                                                                                                        attattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGAt                        >  1:5100997/1‑150 (MQ=255)
                                                                                                                         ttattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTCATTTTCTATAAAGTGAACGAGCTTCATCTCTGATa                       >  2:2442713/1‑150 (MQ=255)
                                                                                                                         ttattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGAGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATa                       <  1:1745745/150‑1 (MQ=255)
                                                                                                                          tattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAg                      <  1:1084151/150‑1 (MQ=255)
                                                                                                                          tattGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAg                      >  1:3627993/1‑150 (MQ=255)
                                                                                                                           attGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGt                     >  1:2897017/1‑150 (MQ=255)
                                                                                                                            ttGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGtt                    <  2:1674517/150‑1 (MQ=255)
                                                                                                                             tGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTg                   <  2:1096975/150‑1 (MQ=255)
                                                                                                                              gACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGa                  <  2:546573/150‑1 (MQ=255)
                                                                                                                               aCGATGAATTTGCATACACTTATAACGACTTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGaa                 <  1:4908044/150‑1 (MQ=255)
                                                                                                                               aCGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGaa                 <  2:2944737/150‑1 (MQ=255)
                                                                                                                                cGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAt                >  1:338976/1‑150 (MQ=255)
                                                                                                                                    gAATTTGCATACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAATAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTaa            >  1:445042/1‑150 (MQ=255)
                                                                                                                                    gAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATCGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTaa            <  1:4267682/150‑1 (MQ=255)
                                                                                                                                     aaTTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAAc           <  2:4208913/150‑1 (MQ=255)
                                                                                                                                       tttGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACtt         <  1:3193626/150‑1 (MQ=255)
                                                                                                                                           cAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCa     >  1:1901269/1‑150 (MQ=255)
                                                                                                                                            aGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAAAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCAt    >  1:2140758/1‑150 (MQ=255)
                                                                                                                                              acacTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGc  >  2:119819/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGC  >  CP009273/4567131‑4567422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑