Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,585,338 A→T 100% S111T (TCT→ACT)  yjjM ← yjjMN operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,585,3380AT99.2% 837.6 / ‑2.9 251S111T (TCT→ACT) yjjMyjjMN operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (117/132);  total (119/132)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 253 total aligned reads displayed.

GCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTC  >  CP009273/4585191‑4585484
                                                                                                                                                   |                                                                                                                                                  
gctgctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGtaa                                                                                                                                                  <  2:4397493/150‑1 (MQ=255)
  tgctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAAc                                                                                                                                                <  1:4626965/150‑1 (MQ=255)
   gctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACg                                                                                                                                               <  1:4432577/150‑1 (MQ=255)
   gctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATATTAAAAGATATTCTCTTACTACAACCTGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACg                                                                                                                                               >  1:5050186/1‑150 (MQ=255)
     tCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGtt                                                                                                                                             <  2:4842754/150‑1 (MQ=255)
      cGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGttt                                                                                                                                            <  1:86075/150‑1 (MQ=255)
        gCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTcc                                                                                                                                          <  1:248637/150‑1 (MQ=255)
           tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCt                                                                                                                                       >  2:2886536/1‑150 (MQ=255)
           tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCt                                                                                                                                       <  2:3814056/150‑1 (MQ=255)
             ggtttGATAGATTTGATTCATGATCCACTGGCCGCGATTTTCGCTGTGAATATTATTATAACGGCCGAATTTTAAAAGATAGTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTgg                                                                                                                                     <  1:4738725/147‑1 (MQ=255)
               aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGc                                                                                                                                   <  2:2367797/150‑1 (MQ=255)
                  gATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                                <  2:4335612/150‑1 (MQ=255)
                  gATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                                >  1:2361983/1‑150 (MQ=255)
                   aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTaa                                                                                                                               <  2:1696039/150‑1 (MQ=255)
                    tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAAc                                                                                                                              >  2:1821879/1‑150 (MQ=255)
                    tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAAc                                                                                                                              <  2:4139941/150‑1 (MQ=255)
                     cGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACt                                                                                                                             >  2:4716139/1‑150 (MQ=255)
                     cGAATGGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACt                                                                                                                             <  2:3607267/150‑1 (MQ=255)
                       aaTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTgg                                                                                                                           <  1:3180357/150‑1 (MQ=255)
                            cTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTg                                                                                                                      >  2:829408/1‑150 (MQ=255)
                            cTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATAGTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTg                                                                                                                      <  1:1071306/150‑1 (MQ=255)
                             ttCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTgg                                                                                                                     >  2:284777/1‑150 (MQ=255)
                               cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGtt                                                                                                                   <  2:3466236/150‑1 (MQ=255)
                               cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGtt                                                                                                                   <  2:4949898/150‑1 (MQ=255)
                                ctGCTCCACTGGCCGCGCTTTTCGCTGTGGCTAAGATTATAACGGCCGAAGTGTCAAAGATATTCGCTTACTACATCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTATAGGTTTCCCCGGGGCGGAACTGGCGCGGGttt                                                                                                                  >  1:4625805/2‑150 (MQ=255)
                                aTGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGttt                                                                                                                  >  1:2057922/1‑150 (MQ=255)
                                 tGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTa                                                                                                                 <  2:4865649/150‑1 (MQ=255)
                                 tGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTa                                                                                                                 <  2:2396175/150‑1 (MQ=255)
                                  gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTat                                                                                                                <  1:1407713/150‑1 (MQ=255)
                                      cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGAGATTCTCTTACTACAACCGGGCTGACCCCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatcatc                                                                                                            <  2:34167/150‑1 (MQ=255)
                                          ggCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGa                                                                                                        >  2:4276493/1‑150 (MQ=255)
                                          ggCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGa                                                                                                        <  2:2737207/150‑1 (MQ=255)
                                          ggCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGa                                                                                                        <  1:4200096/150‑1 (MQ=255)
                                                gcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAg                                                                                                  >  1:2759091/1‑150 (MQ=255)
                                                  ttttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                >  1:3527050/1‑150 (MQ=255)
                                                   tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCt                                                                                               <  1:1653436/150‑1 (MQ=255)
                                                   tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCt                                                                                               <  1:1731386/150‑1 (MQ=255)
                                                   tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCt                                                                                               <  2:144027/150‑1 (MQ=255)
                                                    ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                              <  2:707798/150‑1 (MQ=255)
                                                    ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                              <  2:4123054/150‑1 (MQ=255)
                                                     tCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGc                                                                                             <  2:3321186/150‑1 (MQ=255)
                                                      cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                            <  1:1716800/150‑1 (MQ=255)
                                                      cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                            <  1:4262468/150‑1 (MQ=255)
                                                      cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                            >  2:2411737/1‑150 (MQ=255)
                                                      cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                            >  2:1643831/1‑150 (MQ=255)
                                                      cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                            <  1:4968339/150‑1 (MQ=255)
                                                       gCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                           >  1:986550/1‑150 (MQ=255)
                                                       gCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                           >  2:3922406/1‑150 (MQ=255)
                                                        cTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCa                                                                                          >  2:3709708/1‑150 (MQ=255)
                                                          gtgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa                                                                                        <  1:536943/150‑1 (MQ=255)
                                                          gtgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa                                                                                        >  2:4993663/1‑150 (MQ=255)
                                                                   aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                               >  2:3231362/1‑150 (MQ=255)
                                                                   aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                               <  1:471349/150‑1 (MQ=255)
                                                                     tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtttt                                                                             <  1:2937500/150‑1 (MQ=255)
                                                                      ataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgttttg                                                                            <  1:3317735/150‑1 (MQ=255)
                                                                      ataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgttttg                                                                            <  1:5153628/150‑1 (MQ=255)
                                                                        aaCGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                          >  2:4059852/1‑150 (MQ=255)
                                                                        aaCGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                          >  2:1365049/1‑150 (MQ=255)
                                                                          cGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCa                                                                        >  1:2204718/1‑150 (MQ=255)
                                                                              cGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                    <  1:2091094/150‑1 (MQ=255)
                                                                                    ttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                              >  1:2172273/1‑150 (MQ=255)
                                                                                          gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACTCTTTGTTTTGTTCACTCATTGAAGCGgtag                                                        >  2:1125326/1‑150 (MQ=255)
                                                                                           atatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagt                                                       >  2:1225534/1‑150 (MQ=255)
                                                                                           atatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagt                                                       >  1:1554896/1‑150 (MQ=255)
                                                                                            tatTCTCTTACTACCACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCCTCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagta                                                      >  2:3157846/1‑150 (MQ=255)
                                                                                              ttCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                    >  1:184978/1‑150 (MQ=255)
                                                                                              ttCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                    >  1:3555146/1‑150 (MQ=255)
                                                                                              ttCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                    <  2:2271507/150‑1 (MQ=255)
                                                                                                  ctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg                                                >  1:3451064/1‑150 (MQ=255)
                                                                                                  ctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg                                                >  2:3876831/1‑150 (MQ=255)
                                                                                                   ttACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGa                                               >  2:4177851/1‑150 (MQ=255)
                                                                                                      ctacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAAc                                            <  2:3600449/150‑1 (MQ=255)
                                                                                                      ctacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAAc                                            >  2:4317472/1‑150 (MQ=255)
                                                                                                        acAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCg                                          >  1:653100/1‑150 (MQ=255)
                                                                                                        acAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCg                                          <  1:4847142/150‑1 (MQ=255)
                                                                                                        acAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCg                                          <  2:4187432/150‑1 (MQ=255)
                                                                                                        acAACCCGTCTGACCCCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGAGCTGTTTTACCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCg                                          <  2:2621677/150‑1 (MQ=255)
                                                                                                         cAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGt                                         <  2:2016790/150‑1 (MQ=255)
                                                                                                          aaCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtc                                        <  1:3695467/150‑1 (MQ=255)
                                                                                                            ccGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcat                                      >  1:3410112/1‑150 (MQ=255)
                                                                                                              gggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatca                                    >  1:3423377/1‑150 (MQ=255)
                                                                                                                 cTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATgg                                 <  2:634021/150‑1 (MQ=255)
                                                                                                                 cTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATgg                                 <  2:208499/150‑1 (MQ=255)
                                                                                                                   tacacCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTc                               >  1:2183027/2‑150 (MQ=255)
                                                                                                                    acacCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCGGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCa                              <  1:4005006/150‑1 (MQ=255)
                                                                                                                       cccGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGt                           <  2:3355937/150‑1 (MQ=255)
                                                                                                                       cccGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGt                           >  2:2196430/1‑150 (MQ=255)
                                                                                                                       cccGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGt                           <  1:3791401/150‑1 (MQ=255)
                                                                                                                         cgcgGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGttt                         <  1:1211393/150‑1 (MQ=255)
                                                                                                                         cgcgGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGttt                         <  1:3009483/150‑1 (MQ=255)
                                                                                                                           tggCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGGAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAc                       >  1:2658393/2‑150 (MQ=255)
                                                                                                                            ggCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                      >  1:1608353/1‑150 (MQ=255)
                                                                                                                              cccGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGTCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcgcg                    <  2:4748137/150‑1 (MQ=255)
                                                                                                                              cccGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcgcg                    <  2:2085475/150‑1 (MQ=255)
                                                                                                                                    ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATaa              <  1:1393451/150‑1 (MQ=255)
                                                                                                                                     cAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAAc             >  1:3044725/1‑150 (MQ=255)
                                                                                                                                           cAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTc       <  1:3252028/150‑1 (MQ=255)
                                                                                                                                           cAGTTCAGTAAACGTGTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTc       <  1:3666460/150‑1 (MQ=255)
                                                                                                                                              ttCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGtt    <  1:149368/150‑1 (MQ=255)
                                                                                                                                                cAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTc  <  1:2428484/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTC  >  CP009273/4585191‑4585484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑