Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,588,180 G→T 100% S360* (TCA→TAA)  opgB ← phosphoglycerol transferases I and II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,588,1800GT99.3% 869.4 / ‑2.3 265S360* (TCA→TAA) opgBphosphoglycerol transferases I and II
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (131/132);  total (132/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 265 total aligned reads displayed.

TGCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGCT  >  CP009273/4588033‑4588326
                                                                                                                                                   |                                                                                                                                                  
tGCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGAGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTAc                                                                                                                                                  >  2:1374504/1‑150 (MQ=255)
 gCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACt                                                                                                                                                 <  1:132207/150‑1 (MQ=255)
   ttATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGa                                                                                                                                               >  2:5043334/1‑150 (MQ=255)
      ttGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTc                                                                                                                                            >  1:882261/1‑150 (MQ=255)
       tGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTATTGATTCg                                                                                                                                           <  1:4406431/150‑1 (MQ=255)
         aGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGt                                                                                                                                         <  1:2274589/150‑1 (MQ=255)
         aGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGt                                                                                                                                         <  2:3459541/150‑1 (MQ=255)
           gTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGttt                                                                                                                                       <  2:684023/150‑1 (MQ=255)
            tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTa                                                                                                                                      <  2:2098859/150‑1 (MQ=255)
            tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTa                                                                                                                                      >  1:2811463/1‑150 (MQ=255)
             aTTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTAc                                                                                                                                     >  1:4168715/1‑150 (MQ=255)
             aTTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTAc                                                                                                                                     >  2:1828692/1‑150 (MQ=255)
              tttCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTAcc                                                                                                                                    >  1:2119876/1‑150 (MQ=255)
              tttCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTAcc                                                                                                                                    <  2:5026256/150‑1 (MQ=255)
               ttCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCg                                                                                                                                   >  2:3783148/1‑150 (MQ=255)
                tCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGt                                                                                                                                  <  1:1759013/150‑1 (MQ=255)
                   aCGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCaa                                                                                                                               <  1:4751371/150‑1 (MQ=255)
                     gCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCaaaa                                                                                                                             >  2:4761761/1‑150 (MQ=255)
                     gCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCaaaa                                                                                                                             >  2:2117351/1‑150 (MQ=255)
                          gttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCata                                                                                                                        <  1:1928825/150‑1 (MQ=255)
                                cATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATAtttttt                                                                                                                  >  2:2299646/1‑150 (MQ=255)
                                 aTCGCTAAATGGTTAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTg                                                                                                                 <  1:2305500/150‑1 (MQ=255)
                                 aTCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTg                                                                                                                 >  1:4525651/1‑150 (MQ=255)
                                   cGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCg                                                                                                               >  1:3901042/1‑150 (MQ=255)
                                        aaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTAc                                                                                                          >  2:3213060/1‑150 (MQ=255)
                                         aTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                         <  1:4679420/150‑1 (MQ=255)
                                         aTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                         <  2:3099644/150‑1 (MQ=255)
                                         aTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                         <  1:1556991/150‑1 (MQ=255)
                                          tGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAg                                                                                                        >  1:2390292/1‑150 (MQ=255)
                                          tGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATATTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAg                                                                                                        <  2:1904610/150‑1 (MQ=255)
                                           ggTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAgg                                                                                                       <  2:204293/150‑1 (MQ=255)
                                            gTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGt                                                                                                      <  2:1331914/150‑1 (MQ=255)
                                            gTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGt                                                                                                      <  2:3048939/150‑1 (MQ=255)
                                               agaagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACg                                                                                                   >  2:4525540/1‑150 (MQ=255)
                                                 aagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGCCCCCCCCCACCGACCCAATCATATTTTTTGCGGTTACAGGTACgag                                                                                                 <  1:1737845/150‑1 (MQ=255)
                                                 aagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGCACgag                                                                                                 <  2:3837969/150‑1 (MQ=255)
                                                     gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGAt                                                                                             >  2:2455906/1‑150 (MQ=255)
                                                       cgacgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                           >  1:3263853/1‑150 (MQ=255)
                                                       cgacgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                           <  1:202210/150‑1 (MQ=255)
                                                       cgacgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                           >  1:3123627/1‑150 (MQ=255)
                                                        ggcgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGGGCTGAGTTACTGAGTCGGGTTACCGTCAAGAGCATATTTTTTGCGGTTACAGGTACGAGAGATaaa                                                                                          >  1:1697668/3‑150 (MQ=255)
                                                        gacgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaaa                                                                                          >  1:4840961/1‑150 (MQ=255)
                                                              gACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATc                                                                                    <  1:1628128/150‑1 (MQ=255)
                                                               aCGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATcc                                                                                   >  2:627900/1‑150 (MQ=255)
                                                                 ggTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                           <  2:2766744/124‑1 (MQ=255)
                                                                 ggTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                           >  1:2766744/1‑124 (MQ=255)
                                                                   tATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGt                                                                               >  2:3576056/1‑150 (MQ=255)
                                                                   tATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGt                                                                               >  2:446161/1‑150 (MQ=255)
                                                                    aTCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTg                                                                              >  1:1101388/1‑150 (MQ=255)
                                                                     tCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGa                                                                             <  2:3124623/150‑1 (MQ=255)
                                                                       tatAAACCACGGTGACGCTTTGATTTTGTTGATACACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATg                                                                           <  2:3020238/148‑1 (MQ=255)
                                                                         tAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATggg                                                                         >  1:1205070/1‑150 (MQ=255)
                                                                          aaaCCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGt                                                                        <  2:1823442/150‑1 (MQ=255)
                                                                              cACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCg                                                                    <  1:710956/150‑1 (MQ=255)
                                                                                    gACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTc                                                              <  2:4699404/150‑1 (MQ=255)
                                                                                      cGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAg                                                            >  1:2459667/1‑150 (MQ=255)
                                                                                      cGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAg                                                            >  1:2212342/1‑150 (MQ=255)
                                                                                           tGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaa                                                       >  1:4806135/1‑150 (MQ=255)
                                                                                             aTTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAAc                                                     >  2:2487645/1‑150 (MQ=255)
                                                                                               tttGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAg                                                   >  1:3351596/1‑150 (MQ=255)
                                                                                                 tgttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTg                                                 >  2:397/1‑150 (MQ=255)
                                                                                                 tgttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTg                                                 >  2:517320/1‑150 (MQ=255)
                                                                                                  gttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTga                                                >  2:255591/1‑150 (MQ=255)
                                                                                                  gttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTga                                                <  2:1082030/150‑1 (MQ=255)
                                                                                                        aaaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCg                                          >  2:3776374/1‑150 (MQ=255)
                                                                                                        aaaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCg                                          >  2:880322/1‑150 (MQ=255)
                                                                                                         aaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGc                                         >  2:4311891/1‑150 (MQ=255)
                                                                                                          aCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTCTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCt                                        >  2:1557038/1‑150 (MQ=255)
                                                                                                           cGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTg                                       <  1:4496479/150‑1 (MQ=255)
                                                                                                               gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCt                                   >  1:2696391/1‑150 (MQ=255)
                                                                                                               gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCt                                   >  1:4219342/1‑150 (MQ=255)
                                                                                                                cgATGTTCTCCGGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTg                                  <  2:1661775/150‑1 (MQ=255)
                                                                                                                 gATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGa                                 >  2:4872250/1‑150 (MQ=255)
                                                                                                                   tGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGc                               >  2:3545198/1‑150 (MQ=255)
                                                                                                                    gTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCg                              <  1:3239770/150‑1 (MQ=255)
                                                                                                                    gTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCg                              <  1:974090/150‑1 (MQ=255)
                                                                                                                       ctcCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGa                           >  2:3496697/1‑150 (MQ=255)
                                                                                                                        tcCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGaa                          <  2:4187331/150‑1 (MQ=255)
                                                                                                                          cTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGTGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAg                        >  2:2001716/1‑150 (MQ=255)
                                                                                                                          cTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAg                        >  1:2966132/1‑150 (MQ=255)
                                                                                                                           tGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGc                       >  1:1309064/1‑150 (MQ=255)
                                                                                                                           tGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGc                       >  1:314029/1‑150 (MQ=255)
                                                                                                                               tGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCtt                   <  1:264690/150‑1 (MQ=255)
                                                                                                                                gCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTc                  <  2:2262977/150‑1 (MQ=255)
                                                                                                                                 cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGACTCGCTGACCTGAGCGGGACAGCTCTGCa                 >  2:2960096/1‑150 (MQ=255)
                                                                                                                                 cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCa                 <  2:1195807/150‑1 (MQ=255)
                                                                                                                                 cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCa                 <  2:1280798/150‑1 (MQ=255)
                                                                                                                                 cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCa                 <  2:1697668/150‑1 (MQ=255)
                                                                                                                                  aaCTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaa                <  1:3505406/150‑1 (MQ=255)
                                                                                                                                    cTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAAc              >  1:3233446/1‑150 (MQ=255)
                                                                                                                                    cTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAAc              <  1:2781052/150‑1 (MQ=255)
                                                                                                                                      tACCGCGCTTAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACtt            <  2:497678/150‑1 (MQ=255)
                                                                                                                                         cgcgCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACttttt         <  2:2810714/150‑1 (MQ=255)
                                                                                                                                          gcgcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTc        >  1:1380853/1‑150 (MQ=255)
                                                                                                                                           cgcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTcc       >  1:3621079/1‑150 (MQ=255)
                                                                                                                                            gcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCa      >  2:3245565/1‑150 (MQ=255)
                                                                                                                                             cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCAc     >  2:350550/1‑150 (MQ=255)
                                                                                                                                             cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCAc     <  1:5049838/150‑1 (MQ=255)
                                                                                                                                             cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCAc     <  1:2491068/150‑1 (MQ=255)
                                                                                                                                             cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGAGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCAc     <  1:534974/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGCT  >  CP009273/4588033‑4588326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑