Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,591,139 C→A 100% D66Y (GAT→TAT)  dnaT ← DNA biosynthesis protein (primosomal protein I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,591,1390CA99.3% 1010.8 / ‑2.3 311D66Y (GAT→TAT) dnaTDNA biosynthesis protein (primosomal protein I)
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (131/176);  total (133/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 311 total aligned reads displayed.

TCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTA  >  CP009273/4590994‑4591285
                                                                                                                                                 |                                                                                                                                                  
tctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGACTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCtt                                                                                                                                                <  1:2885165/150‑1 (MQ=255)
  tctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTcc                                                                                                                                              >  1:3028096/1‑150 (MQ=255)
  tctTAGCGCCACGCCCCAGAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGGTGATCGTCCAGAGCGACATAGCTTcc                                                                                                                                              <  2:4060306/150‑1 (MQ=255)
   ctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTccc                                                                                                                                             <  1:1910100/150‑1 (MQ=255)
    ttAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCg                                                                                                                                            <  1:3754044/150‑1 (MQ=255)
     tAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCgg                                                                                                                                           <  1:4359375/150‑1 (MQ=255)
      aGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGa                                                                                                                                          >  1:2907590/1‑150 (MQ=255)
        cgcCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcg                                                                                                                                        >  2:549584/1‑150 (MQ=255)
          ccACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgcg                                                                                                                                      >  2:748433/1‑150 (MQ=255)
          ccACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgcg                                                                                                                                      >  1:4774196/1‑150 (MQ=255)
          ccACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgcg                                                                                                                                      <  2:4178157/150‑1 (MQ=255)
          ccACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgcg                                                                                                                                      >  2:2628640/1‑150 (MQ=255)
          ccACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgcg                                                                                                                                      >  2:2703269/1‑150 (MQ=255)
           cACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACgcgc                                                                                                                                     >  1:1739520/1‑150 (MQ=255)
           cACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACgcgc                                                                                                                                     >  2:1953056/1‑150 (MQ=255)
                cccATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCt                                                                                                                                >  1:3514844/1‑150 (MQ=255)
                  cATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCttt                                                                                                                              >  2:772350/1‑150 (MQ=255)
                  cATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCttt                                                                                                                              <  2:1687209/150‑1 (MQ=255)
                  cATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCttt                                                                                                                              >  2:488597/1‑150 (MQ=255)
                     aGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTttct                                                                                                                           >  1:241104/1‑150 (MQ=255)
                     aGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTttct                                                                                                                           >  1:4216868/1‑150 (MQ=255)
                       cgccgcCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTttcttc                                                                                                                         <  1:1255388/150‑1 (MQ=255)
                         ccgccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCa                                                                                                                       >  2:2740170/1‑150 (MQ=255)
                              aGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGcc                                                                                                                  <  2:3788216/150‑1 (MQ=255)
                              aGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGcc                                                                                                                  >  2:4616144/1‑150 (MQ=255)
                               ggCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCca                                                                                                                 <  1:1131836/150‑1 (MQ=255)
                                     tAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGtt                                                                                                           <  1:2149618/150‑1 (MQ=255)
                                       aaaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCa                                                                                                         <  1:2857487/150‑1 (MQ=255)
                                        aaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAg                                                                                                        >  1:3339337/1‑150 (MQ=255)
                                        aaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAg                                                                                                        >  2:3927940/1‑150 (MQ=255)
                                         aTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGc                                                                                                       >  1:555469/1‑150 (MQ=255)
                                          tCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGcc                                                                                                      <  2:917703/150‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCca                                                                                                     <  2:2448254/150‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCca                                                                                                     <  2:1221631/150‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCca                                                                                                     <  2:3344261/150‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCca                                                                                                     <  1:4944023/150‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCca                                                                                                     <  1:3441512/150‑1 (MQ=255)
                                            ggCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcag                                                                                                    <  1:1350907/150‑1 (MQ=255)
                                             gCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcagg                                                                                                   >  2:4412101/1‑150 (MQ=255)
                                                 cGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtg                                                                                               >  1:3062690/1‑150 (MQ=255)
                                                    gTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcag                                                                                            <  2:4936109/150‑1 (MQ=255)
                                                    gTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcag                                                                                            <  2:1580136/150‑1 (MQ=255)
                                                      tGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggc                                                                                          >  1:4673658/1‑150 (MQ=255)
                                                       gCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggcg                                                                                         >  2:5124227/1‑150 (MQ=255)
                                                         cAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggcgtg                                                                                       <  2:2914431/150‑1 (MQ=255)
                                                            tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACg                                                                                    <  1:1322097/150‑1 (MQ=255)
                                                            tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACg                                                                                    >  2:3538717/1‑150 (MQ=255)
                                                            tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACg                                                                                    <  1:2372740/150‑1 (MQ=255)
                                                             ccGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACgg                                                                                   <  1:94510/150‑1 (MQ=255)
                                                               ggATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                                 <  2:1912852/150‑1 (MQ=255)
                                                               ggATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                                 >  2:4260135/1‑150 (MQ=255)
                                                                  tACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATaa                                                                              <  1:1468435/150‑1 (MQ=255)
                                                                     aTGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAAc                                                                           <  1:3925609/150‑1 (MQ=255)
                                                                      tGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACg                                                                          <  2:2989847/150‑1 (MQ=255)
                                                                      tGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACg                                                                          <  1:5152062/150‑1 (MQ=255)
                                                                        gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGcc                                                                        <  2:3522832/150‑1 (MQ=255)
                                                                        gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGcc                                                                        <  2:2050112/150‑1 (MQ=255)
                                                                         cGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCg                                                                       <  2:3100644/150‑1 (MQ=255)
                                                                          gAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgg                                                                      >  2:2308493/1‑150 (MQ=255)
                                                                             tttCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGc                                                                   <  2:3356712/150‑1 (MQ=255)
                                                                                 cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTg                                                               >  1:3709337/1‑150 (MQ=255)
                                                                                 cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTg                                                               <  2:1281386/150‑1 (MQ=255)
                                                                                    aTGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTa                                                            <  2:1334232/150‑1 (MQ=255)
                                                                                     tGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAg                                                           <  1:5073387/150‑1 (MQ=255)
                                                                                            aGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc                                                    <  1:2735995/150‑1 (MQ=255)
                                                                                             gCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACg                                                   <  1:1796317/150‑1 (MQ=255)
                                                                                             gCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACg                                                   <  2:3198844/150‑1 (MQ=255)
                                                                                              cAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACgg                                                  <  2:579512/150‑1 (MQ=255)
                                                                                              cAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACgg                                                  <  2:181690/150‑1 (MQ=255)
                                                                                               aaCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCCTTGTTAGCAAATACGGc                                                 >  2:4314238/1‑150 (MQ=255)
                                                                                                 cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCaa                                               <  2:2476445/150‑1 (MQ=255)
                                                                                                 cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCaa                                               <  2:3223113/150‑1 (MQ=255)
                                                                                                  gggAGCGGCGTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAAc                                              <  2:4327658/150‑1 (MQ=255)
                                                                                                    gAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACca                                            <  2:805124/150‑1 (MQ=255)
                                                                                                        ggCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACAcc                                        <  1:1671602/150‑1 (MQ=255)
                                                                                                            tGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCTCCTGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt                                    <  1:2035025/150‑1 (MQ=255)
                                                                                                            tGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt                                    >  2:212826/1‑150 (MQ=255)
                                                                                                            tGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt                                    <  1:1850186/150‑1 (MQ=255)
                                                                                                             gCGGTTCCTGATAGAGTTGATCGTCCAGAGCTAAATAGCTTCCCGGACGCGCCAGATTTTCTTCCAGCGCCAGCATTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCtt                                   <  1:1023565/150‑1 (MQ=255)
                                                                                                             gCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCtt                                   >  1:158231/1‑150 (MQ=255)
                                                                                                              cGGTTCCTGAGAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTc                                  <  2:2264094/150‑1 (MQ=255)
                                                                                                               ggTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCa                                 <  2:241104/150‑1 (MQ=255)
                                                                                                                gTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg                                >  2:946976/1‑150 (MQ=255)
                                                                                                                 ttCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGc                               >  2:312852/1‑150 (MQ=255)
                                                                                                                   ccTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtt                             <  1:1371478/150‑1 (MQ=255)
                                                                                                                      gATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTg                          >  2:148040/1‑150 (MQ=255)
                                                                                                                       aTAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc                         >  2:10740/1‑150 (MQ=255)
                                                                                                                          gagTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg                      <  1:2703269/150‑1 (MQ=255)
                                                                                                                          gagTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg                      >  1:2957634/1‑150 (MQ=255)
                                                                                                                           agTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa                     <  1:2591738/150‑1 (MQ=255)
                                                                                                                             ttGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAAc                   <  2:434454/150‑1 (MQ=255)
                                                                                                                              tGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACg                  <  1:1347255/150‑1 (MQ=255)
                                                                                                                                   gTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTg             >  1:3225480/1‑150 (MQ=255)
                                                                                                                                     ccAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGt           <  1:772350/150‑1 (MQ=255)
                                                                                                                                     ccAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGt           <  1:488597/150‑1 (MQ=255)
                                                                                                                                      cAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg          >  1:3910756/1‑150 (MQ=255)
                                                                                                                                      cAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg          >  1:4293593/1‑150 (MQ=255)
                                                                                                                                       agagCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCATGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa         >  1:4056468/1‑150 (MQ=255)
                                                                                                                                            gACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCgtg    >  1:573592/1‑150 (MQ=255)
                                                                                                                                              cATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTa  <  2:536988/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTA  >  CP009273/4590994‑4591285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑