Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,625,288 T→C 100% intergenic (‑161/‑50) rob ← / → creA right oriC‑binding transcriptional activator, AraC family/putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,625,2880TC100.0% 859.1 / ‑6.7 222intergenic (‑161/‑50)rob/creAright oriC‑binding transcriptional activator, AraC family/putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (107/114);  minor base A (1/0);  total (108/114)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

CCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAA  >  CP009273/4625141‑4625434
                                                                                                                                                   |                                                                                                                                                  
ccTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGAGTTTGTTCTTAATCAATATGTTActt                                                                                                                                                  >  2:3938688/1‑150 (MQ=255)
    tCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCg                                                                                                                                              >  2:4517456/1‑150 (MQ=255)
     caacaaCGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGt                                                                                                                                             >  1:2993333/1‑150 (MQ=255)
      aacaacGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTg                                                                                                                                            <  2:54129/150‑1 (MQ=255)
         aacGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGAca                                                                                                                                         <  1:1686388/150‑2 (MQ=255)
            gAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAAc                                                                                                                                      <  1:414901/150‑1 (MQ=255)
              gCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTCGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTa                                                                                                                                    >  1:440048/1‑150 (MQ=255)
              gCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTa                                                                                                                                    <  2:2154279/150‑1 (MQ=255)
               cACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTaa                                                                                                                                   >  2:3597669/1‑150 (MQ=255)
                 ccTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAAtt                                                                                                                                 <  1:1573461/150‑1 (MQ=255)
                 ccTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAAtt                                                                                                                                 <  1:1573753/150‑1 (MQ=255)
                  cTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTg                                                                                                                                >  2:2271444/1‑150 (MQ=255)
                    gtaaTCATGTAATTGGTTTATTTGATTTTTTTGTTTTTTTTGATTCACTTTTTTTTTAATATTATTGTATTTTTTTTGGGTTGTAGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCt                                                                                                                              <  2:4884303/147‑1 (MQ=255)
                     aCATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTc                                                                                                                             <  1:2159321/150‑1 (MQ=255)
                        tCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCgtg                                                                                                                          >  1:3493112/1‑150 (MQ=255)
                        tCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCgtg                                                                                                                          <  2:2978105/150‑1 (MQ=255)
                        tCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATAAGTTACTTACCGTGACGAACTAATTGCTCgtg                                                                                                                          >  1:1226637/1‑150 (MQ=255)
                         cAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCgtgt                                                                                                                         <  2:4293675/150‑1 (MQ=255)
                         cAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCgtgt                                                                                                                         <  2:847969/150‑1 (MQ=255)
                          aGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTa                                                                                                                        <  1:5073867/150‑1 (MQ=255)
                           ggTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTaa                                                                                                                       <  2:2820327/150‑1 (MQ=255)
                             tAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATa                                                                                                                     <  2:1474507/150‑1 (MQ=255)
                             tAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATa                                                                                                                     <  2:2488784/150‑1 (MQ=255)
                              aaTTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAg                                                                                                                    <  2:1168321/150‑1 (MQ=255)
                              aaTTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAg                                                                                                                    <  2:5078462/150‑1 (MQ=255)
                                  ggATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATaa                                                                                                                >  1:1975916/1‑150 (MQ=255)
                                    ataataGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATaaaa                                                                                                              >  1:4984378/1‑150 (MQ=255)
                                    ataataGCATTTTTTGCTGTTTTAGCATTCAGAGTTTTTTTCTTAGTAGAGTATGTTTTAGTGCTTGACTGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAAATAATTGCTCGTGTAATAGATaaaa                                                                                                              >  1:979291/1‑150 (MQ=255)
                                      aataGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAAt                                                                                                            >  1:4263119/1‑150 (MQ=255)
                                       ataGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATg                                                                                                           <  1:1845475/150‑1 (MQ=255)
                                        taGCATTTTTTGCTGTTTTAGCATTCTGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTTAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATgg                                                                                                          <  2:4480801/150‑1 (MQ=255)
                                               ttttGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAat                                                                                                   <  2:12322/150‑1 (MQ=255)
                                                 ttGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAatat                                                                                                 <  1:3194390/150‑1 (MQ=255)
                                                 ttGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAatat                                                                                                 >  1:1386534/1‑150 (MQ=255)
                                                    cTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGaa                                                                                              >  1:3140368/1‑150 (MQ=255)
                                                    cTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGaa                                                                                              >  1:2604736/1‑150 (MQ=255)
                                                    cTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGaa                                                                                              >  1:2604662/1‑150 (MQ=255)
                                                         ttAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACa                                                                                         <  1:1114102/150‑1 (MQ=255)
                                                          tAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACaa                                                                                        >  2:2739874/1‑150 (MQ=255)
                                                          tAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACaa                                                                                        >  2:3261188/1‑150 (MQ=255)
                                                           aGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAg                                                                                       <  2:904201/150‑1 (MQ=255)
                                                              aTTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATTTTAATTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATCTGAAATACAAGCAt                                                                                    >  2:2612178/1‑150 (MQ=255)
                                                              aTTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAt                                                                                    >  1:2508313/1‑150 (MQ=255)
                                                               ttCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAtt                                                                                   >  2:4374083/1‑150 (MQ=255)
                                                               ttCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAtt                                                                                   >  2:2354135/1‑150 (MQ=255)
                                                                tCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAttt                                                                                  <  1:1743384/150‑1 (MQ=255)
                                                                tCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAttt                                                                                  >  2:2120093/1‑150 (MQ=255)
                                                                 cAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTg                                                                                 <  1:762629/150‑1 (MQ=255)
                                                                   gtgtTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGAt                                                                               <  2:3101819/150‑1 (MQ=255)
                                                                    tgtTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATc                                                                              >  1:3450454/1‑150 (MQ=255)
                                                                      tttttttCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCt                                                                            <  1:949458/150‑1 (MQ=255)
                                                                       ttttttCTTAGTAGAGTATGTTTTAGGGCTTGACGTAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTg                                                                           <  2:1958824/150‑1 (MQ=255)
                                                                         ttttCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTc                                                                         <  2:1813082/150‑1 (MQ=255)
                                                                         ttttCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTATTAATCAATATGTTACTTACAGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCAATTGATCCTGTc                                                                         >  1:4486013/1‑150 (MQ=255)
                                                                            tCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCttt                                                                      >  2:1500872/1‑150 (MQ=255)
                                                                             ctgagtagAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTa                                                                     <  2:4659194/147‑1 (MQ=255)
                                                                              ttAGTAGAGTATGTTTTAGGGATTGACTGAAATAAAAGTATTGAGATTTTTTTCTTAATCAATATTTTACTTACCTTGACGAAATATTTGCTCGTTTAATAGATAAAAATGGTAACAATATGAAATACAAGAATTTGATCCTGTCTTTaa                                                                    <  2:1986625/150‑1 (MQ=255)
                                                                                  tagAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCt                                                                >  1:734094/1‑150 (MQ=255)
                                                                                   agagTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTg                                                               >  1:3815887/1‑150 (MQ=255)
                                                                                   agagTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTg                                                               >  2:1719054/1‑150 (MQ=255)
                                                                                     agTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGAt                                                             >  2:1415932/1‑150 (MQ=255)
                                                                                        aTGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAAt                                                          <  1:2913522/150‑1 (MQ=255)
                                                                                         tGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATg                                                         >  1:1517254/1‑150 (MQ=255)
                                                                                         tGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATg                                                         >  2:2641382/1‑150 (MQ=255)
                                                                                              tAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTggg                                                    <  1:4031409/150‑1 (MQ=255)
                                                                                              tAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTggg                                                    <  2:5004980/150‑1 (MQ=255)
                                                                                                gggCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGc                                                  >  2:2230978/1‑150 (MQ=255)
                                                                                                 ggCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGcc                                                 >  1:4991406/1‑150 (MQ=255)
                                                                                                   cTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCAt                                               >  1:3536256/1‑150 (MQ=255)
                                                                                                    ttGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCAtt                                              <  2:350297/150‑1 (MQ=255)
                                                                                                      gACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTgg                                            >  1:2824313/1‑150 (MQ=255)
                                                                                                       aCGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGc                                           <  1:2381147/150‑1 (MQ=255)
                                                                                                         ggAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTACTAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTc                                         >  2:4362323/1‑150 (MQ=255)
                                                                                                           aaaTAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCAt                                       >  2:5055991/1‑150 (MQ=255)
                                                                                                           aaaTAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCAt                                       >  2:339328/1‑150 (MQ=255)
                                                                                                             aTAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGc                                     >  1:2427258/1‑150 (MQ=255)
                                                                                                              tAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCa                                    >  2:1663110/1‑150 (MQ=255)
                                                                                                              tAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCa                                    >  2:3775261/1‑150 (MQ=255)
                                                                                                              tAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCa                                    <  1:536110/150‑1 (MQ=255)
                                                                                                              tAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCa                                    <  1:3123799/150‑1 (MQ=255)
                                                                                                                 aaGTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCagaa                                 >  1:820327/1‑150 (MQ=255)
                                                                                                                   gTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGTTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCagaaga                               >  2:130967/1‑150 (MQ=255)
                                                                                                                   gTATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGACTGATAATGCTGGGGACATTGGCTCATGCagacga                               >  2:4460930/1‑150 (MQ=255)
                                                                                                                    tATTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAagag                              >  2:4171229/1‑150 (MQ=255)
                                                                                                                     aTTGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAgaga                             <  1:1854474/150‑1 (MQ=255)
                                                                                                                      ttGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGAt                            >  1:2887395/1‑150 (MQ=255)
                                                                                                                       tGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGAtt                           <  1:2253610/150‑1 (MQ=255)
                                                                                                                       tGAGATTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGAtt                           <  1:1467178/150‑1 (MQ=255)
                                                                                                                        gagaTTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTg                          >  1:3889611/1‑150 (MQ=255)
                                                                                                                           aTTTTGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGtt                       >  1:217497/1‑150 (MQ=255)
                                                                                                                             tttGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCg                     <  1:4257791/150‑1 (MQ=255)
                                                                                                                              ttGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCgg                    <  1:27379/150‑1 (MQ=255)
                                                                                                                               tGTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGt                   >  1:3161241/1‑150 (MQ=255)
                                                                                                                                gTTCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTc                  >  2:408981/1‑150 (MQ=255)
                                                                                                                                  tCTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGa                <  1:5083799/150‑1 (MQ=255)
                                                                                                                                   cTTAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGac               <  1:2062287/150‑1 (MQ=255)
                                                                                                                                     tAATCAATATGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGacac             >  1:1404442/1‑150 (MQ=255)
                                                                                                                                            tatGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTAttt      <  2:1847256/150‑1 (MQ=255)
                                                                                                                                             atGTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTa     >  1:3331970/1‑150 (MQ=255)
                                                                                                                                               gTTACTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTaaa   <  2:2574760/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAA  >  CP009273/4625141‑4625434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑