Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 390,551 T→G 100% L197R (CTG→CGG)  yaiV → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273390,5510TG96.3% 1070.9 / 10.8 282L197R (CTG→CGG) yaiVputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/8);  new base G (154/117);  total (157/125)
Fisher's exact test for biased strand distribution p-value = 6.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 282 total aligned reads displayed.

TCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390403‑390697
                                                                                                                                                    |                                                                                                                                                  
tCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTg                                                                                                                                                   <  1:3777907/150‑1 (MQ=255)
  aaTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATCAAGGCAAACGGGt                                                                                                                                                 >  2:4740965/1‑150 (MQ=255)
   aTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTc                                                                                                                                                <  1:137496/150‑1 (MQ=255)
   aTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTc                                                                                                                                                <  1:526943/150‑1 (MQ=255)
   aTGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTc                                                                                                                                                <  1:3725279/150‑1 (MQ=255)
    tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                               <  1:879307/150‑1 (MQ=255)
    tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                               <  2:4163169/150‑1 (MQ=255)
    tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                               >  1:5005459/1‑150 (MQ=255)
    tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                               <  1:4983393/150‑1 (MQ=255)
      aTTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCt                                                                                                                                             <  2:4547735/150‑1 (MQ=255)
       tttaCTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACTCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTa                                                                                                                                            >  2:2075199/4‑150 (MQ=255)
       ttGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTa                                                                                                                                            >  1:1300486/1‑150 (MQ=255)
       ttGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTa                                                                                                                                            >  1:3883593/1‑150 (MQ=255)
        tGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTAt                                                                                                                                           <  1:1269713/150‑1 (MQ=255)
         gACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATc                                                                                                                                          <  2:2835965/150‑1 (MQ=255)
           cTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCaa                                                                                                                                        >  2:3273207/1‑150 (MQ=255)
            tGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAc                                                                                                                                       >  1:2629373/1‑150 (MQ=255)
               aaTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAATGGCACACGGGTCGCTATCAACCGt                                                                                                                                    >  2:2544600/1‑150 (MQ=255)
               aaTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                    >  2:2733696/1‑150 (MQ=255)
                aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGtt                                                                                                                                   >  1:2454214/1‑150 (MQ=255)
                 tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGttt                                                                                                                                  <  1:316674/150‑1 (MQ=255)
                 tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGttt                                                                                                                                  <  1:182891/150‑1 (MQ=255)
                  gaagaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                                 >  2:1435475/1‑150 (MQ=255)
                   aagaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                                <  2:1800776/150‑1 (MQ=255)
                       aTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTc                                                                                                                            <  1:2839923/150‑1 (MQ=255)
                       aTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTc                                                                                                                            >  1:2920862/1‑150 (MQ=255)
                         tGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCag                                                                                                                          >  1:3371518/1‑150 (MQ=255)
                          gCGATCACGTATGGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGGTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCaga                                                                                                                         <  2:2348701/150‑1 (MQ=255)
                           cGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCagag                                                                                                                        >  2:3617723/1‑150 (MQ=255)
                            gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       >  2:1332628/1‑150 (MQ=255)
                            gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGt                                                                                                                       <  2:4254601/150‑1 (MQ=255)
                                   tATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATc                                                                                                                >  2:431438/1‑150 (MQ=255)
                                         tgtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcg                                                                                                          <  2:505726/150‑1 (MQ=255)
                                                taTTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGAt                                                                                                   >  2:1930993/2‑150 (MQ=255)
                                                aaTTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGAt                                                                                                   >  1:4213149/1‑150 (MQ=255)
                                                      atCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCaa                                                                                             <  2:2895542/150‑1 (MQ=255)
                                                            cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       >  1:4578288/1‑150 (MQ=255)
                                                            cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGttt                                                                                       >  2:926533/1‑150 (MQ=255)
                                                              aCGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAt                                                                                     >  1:2562984/1‑150 (MQ=255)
                                                               cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTGTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                    >  1:2670776/1‑150 (MQ=255)
                                                               cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                    >  1:2976245/1‑150 (MQ=255)
                                                               cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                    >  1:45396/1‑150 (MQ=255)
                                                               cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                    >  1:712840/1‑150 (MQ=255)
                                                                gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTCTATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAGTAATCAGGACGCGGGGATAACCCACGGTTTATTc                                                                                   >  2:2484633/1‑150 (MQ=255)
                                                                gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTc                                                                                   >  1:2142898/1‑150 (MQ=255)
                                                                 tACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTcc                                                                                  >  1:3927002/1‑150 (MQ=255)
                                                                 tACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTcc                                                                                  >  2:1232692/1‑150 (MQ=255)
                                                                  acacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTccc                                                                                 <  2:3006438/150‑1 (MQ=255)
                                                                   cacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCg                                                                                >  2:959881/1‑150 (MQ=255)
                                                                   cacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCg                                                                                >  2:2585467/1‑150 (MQ=255)
                                                                     cGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCAGCt                                                                              <  1:2536855/150‑1 (MQ=255)
                                                                       cATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTa                                                                            <  2:2017030/150‑1 (MQ=255)
                                                                       cATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTa                                                                            <  2:1140745/150‑1 (MQ=255)
                                                                         tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGc                                                                          >  2:1508637/1‑150 (MQ=255)
                                                                         tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTATTCCTTCAGAGTATTAATCAGGACGCGGGGATAAAAAACGGTTTATTCCAGCTTAGc                                                                          >  1:1966437/1‑150 (MQ=255)
                                                                          atCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCt                                                                         >  1:2226952/1‑150 (MQ=255)
                                                                           tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTc                                                                        >  2:3732314/1‑150 (MQ=255)
                                                                           tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTc                                                                        >  1:4138449/1‑150 (MQ=255)
                                                                             gcgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCgg                                                                      <  2:3704991/150‑1 (MQ=255)
                                                                                     tcgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAgg                                                              <  2:5019503/150‑1 (MQ=255)
                                                                                     tcgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAgg                                                              >  2:4055834/1‑150 (MQ=255)
                                                                                      cgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGt                                                             >  2:2053464/1‑150 (MQ=255)
                                                                                        tcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCa                                                           <  2:56785/150‑1 (MQ=255)
                                                                                        tcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAGTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCa                                                           >  2:4877154/1‑150 (MQ=255)
                                                                                         cgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAt                                                          >  2:4893631/1‑150 (MQ=255)
                                                                                          gCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAtt                                                         <  1:1019269/150‑1 (MQ=255)
                                                                                             gAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGAt                                                      <  2:3884095/150‑1 (MQ=255)
                                                                                                gTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGcc                                                   >  2:2538999/1‑150 (MQ=255)
                                                                                                 ttCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCa                                                  <  2:4810104/150‑1 (MQ=255)
                                                                                                 ttCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCa                                                  <  2:743136/150‑1 (MQ=255)
                                                                                                  tctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCAt                                                 <  1:4704316/150‑1 (MQ=255)
                                                                                                   ctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATc                                                <  2:3954378/150‑1 (MQ=255)
                                                                                                       ctgctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTc                                            <  2:3471465/150‑1 (MQ=255)
                                                                                                        tgctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCa                                           <  1:1548670/150‑1 (MQ=255)
                                                                                                                  aaaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTACCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTaaa                                 >  1:3996733/1‑150 (MQ=255)
                                                                                                                   aaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAg                                >  1:1124768/1‑150 (MQ=255)
                                                                                                                   aaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAg                                >  2:3392268/1‑150 (MQ=255)
                                                                                                                         gcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTc                          >  1:2121519/1‑150 (MQ=255)
                                                                                                                         gcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTc                          <  1:655446/150‑1 (MQ=255)
                                                                                                                          cTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCa                         <  2:1653563/150‑1 (MQ=255)
                                                                                                                           tataTCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                        >  1:482545/1‑150 (MQ=255)
                                                                                                                            atatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAAc                       <  1:1383278/150‑1 (MQ=255)
                                                                                                                              atCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACgg                     >  1:3091961/1‑150 (MQ=255)
                                                                                                                               tCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGc                    <  1:2350550/150‑1 (MQ=255)
                                                                                                                                cGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCg                   <  1:3235988/150‑1 (MQ=255)
                                                                                                                                 gAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                  >  1:3316677/1‑150 (MQ=255)
                                                                                                                                      gAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAg             <  2:4697093/150‑1 (MQ=255)
                                                                                                                                      gAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAg             <  1:3067669/150‑1 (MQ=255)
                                                                                                                                       aaTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt            <  1:4366247/150‑1 (MQ=255)
                                                                                                                                        aTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTg           >  2:4143113/1‑150 (MQ=255)
                                                                                                                                        aTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTg           <  2:2414145/150‑1 (MQ=255)
                                                                                                                                        aTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTGTTAAAGCGCGTCAACGGCGAACGAGTg           >  2:3023781/1‑150 (MQ=255)
                                                                                                                                         tAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGa          >  1:5081152/1‑150 (MQ=255)
                                                                                                                                         tAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGa          <  1:1435475/150‑1 (MQ=255)
                                                                                                                                            aGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacca       >  1:3428360/1‑150 (MQ=255)
                                                                                                                                            aGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacca       <  2:1865811/150‑1 (MQ=255)
                                                                                                                                             ggCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGaccac      >  1:398802/1‑150 (MQ=255)
                                                                                                                                              gCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGaccacc     >  2:492192/1‑150 (MQ=255)
                                                                                                                                              gCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGaccacc     >  1:942067/1‑150 (MQ=255)
                                                                                                                                              gCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGaccacc     >  1:990856/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390403‑390697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑