Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 397,959 G→A 100% A253T (GCG→ACG)  phoA → bacterial alkaline phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273397,9590GA98.7% 1004.5 / 1.1 301A253T (GCG→ACG) phoAbacterial alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base A (181/116);  total (185/116)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 311 total aligned reads displayed.

CTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATC  >  CP009273/397813‑398105
                                                                                                                                                  |                                                                                                                                                  
ctctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGacgc                                                                                                                                                 >  1:4287289/1‑150 (MQ=255)
 tctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGACAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGAcgcg                                                                                                                                                <  2:4015425/150‑1 (MQ=255)
  ctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGt                                                                                                                                               <  2:4483801/150‑1 (MQ=255)
  ctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGt                                                                                                                                               >  1:466053/1‑150 (MQ=255)
   tGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTg                                                                                                                                              <  1:3257148/150‑1 (MQ=255)
    tgAAATAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTgg                                                                                                                                             >  2:2633759/2‑150 (MQ=255)
     gAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGt                                                                                                                                            <  2:4214106/150‑1 (MQ=255)
      aaaaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGtt                                                                                                                                           >  1:5095399/1‑150 (MQ=255)
      aaaaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGtt                                                                                                                                           >  1:436213/1‑150 (MQ=255)
       aaaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTa                                                                                                                                          <  2:2523838/150‑1 (MQ=255)
       aaaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTa                                                                                                                                          >  2:305798/1‑150 (MQ=255)
            gCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGt                                                                                                                                     <  2:3161535/150‑1 (MQ=255)
             cGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGtt                                                                                                                                    >  1:2852548/1‑150 (MQ=255)
              ggAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTg                                                                                                                                   >  1:2034680/1‑150 (MQ=255)
              ggAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTg                                                                                                                                   >  2:1812349/1‑150 (MQ=255)
              ggAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTg                                                                                                                                   >  1:1753655/1‑150 (MQ=255)
               gAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTgg                                                                                                                                  >  1:1586101/1‑150 (MQ=255)
                  aaGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGa                                                                                                                               >  1:967113/1‑150 (MQ=255)
                   aGGATTGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAg                                                                                                                              >  1:2323566/1‑150 (MQ=255)
                    ggATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCGGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGc                                                                                                                             >  1:3064640/1‑150 (MQ=255)
                     gATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCg                                                                                                                            >  2:4769968/1‑150 (MQ=255)
                     gATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCg                                                                                                                            >  2:4958492/1‑150 (MQ=255)
                     gATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCg                                                                                                                            >  1:3289790/1‑150 (MQ=255)
                     gATCGATGACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCg                                                                                                                            <  1:3497576/150‑1 (MQ=255)
                      aTCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGa                                                                                                                           >  1:3749276/1‑150 (MQ=255)
                       tCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAt                                                                                                                          <  1:4782329/150‑1 (MQ=255)
                       tCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAt                                                                                                                          >  1:4151924/1‑150 (MQ=255)
                        cGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtg                                                                                                                         <  1:4706362/150‑1 (MQ=255)
                           ttACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtgctg                                                                                                                      >  1:1283432/1‑150 (MQ=255)
                            tACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtgctgc                                                                                                                     >  2:410789/1‑150 (MQ=255)
                             aCCGAACAGCTGCTTAACGCTCATGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGcc                                                                                                                    <  1:3199093/150‑1 (MQ=255)
                               cGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTc                                                                                                                  >  1:241076/1‑150 (MQ=255)
                                gAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCa                                                                                                                 >  1:1333087/1‑150 (MQ=255)
                                gAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCa                                                                                                                 >  1:5113235/1‑150 (MQ=255)
                                  aCAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACt                                                                                                               <  2:620937/150‑1 (MQ=255)
                                       tgctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAAtt                                                                                                          >  1:1126909/1‑150 (MQ=255)
                                        gctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTc                                                                                                         <  2:2974868/150‑1 (MQ=255)
                                         ctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCg                                                                                                        >  1:1535308/1‑150 (MQ=255)
                                          ttAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCgg                                                                                                       <  1:4024151/150‑1 (MQ=255)
                                           tAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGt                                                                                                      >  1:1853015/1‑150 (MQ=255)
                                           tAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGt                                                                                                      >  1:1402164/1‑150 (MQ=255)
                                           tAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGAAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGt                                                                                                      <  1:2223454/150‑1 (MQ=255)
                                             aCGCTCGTGCCGTCGTTACGCTTGGCTGCGGCGCAAAAAACTTTGCTGATACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCTTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGtta                                                                                                    <  1:4971950/150‑3 (MQ=255)
                                             aCGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGa                                                                                                    <  2:167052/150‑1 (MQ=255)
                                              cGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGAc                                                                                                   >  2:1183550/1‑150 (MQ=255)
                                              cGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGAc                                                                                                   >  2:3368401/1‑150 (MQ=255)
                                                  cGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGaa                                                                                               <  2:3601294/150‑1 (MQ=255)
                                                      ccGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGa                                                                                           <  2:2804805/150‑1 (MQ=255)
                                                       cGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGaa                                                                                          <  2:1959457/150‑1 (MQ=255)
                                                         aCGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATc                                                                                        <  1:2124175/150‑1 (MQ=255)
                                                          cGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCa                                                                                       >  2:4998493/1‑150 (MQ=255)
                                                             tACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCa                                                                                    >  1:5154853/1‑150 (MQ=255)
                                                             tACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCa                                                                                    <  2:5042352/150‑1 (MQ=255)
                                                              aCGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaa                                                                                   >  1:106410/1‑150 (MQ=255)
                                                               cGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaa                                                                                  >  1:2189571/1‑150 (MQ=255)
                                                               cGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaa                                                                                  >  2:3282340/1‑150 (MQ=255)
                                                                 cTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaaaa                                                                                >  1:36838/1‑150 (MQ=255)
                                                                 cTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaaaa                                                                                <  1:4313029/150‑1 (MQ=255)
                                                                     gcggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAAcccc                                                                            <  1:843228/150‑1 (MQ=255)
                                                                      cggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCCCAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCt                                                                           >  2:1628866/1‑150 (MQ=255)
                                                                      cggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCt                                                                           <  2:419245/150‑1 (MQ=255)
                                                                       ggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTg                                                                          >  2:1337828/1‑150 (MQ=255)
                                                                        gcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGc                                                                         <  2:4279752/150‑1 (MQ=255)
                                                                           gcgcAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTg                                                                      <  2:1256780/150‑1 (MQ=255)
                                                                            cgcAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTgg                                                                     >  1:257553/1‑150 (MQ=255)
                                                                               aaaaaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCt                                                                  <  2:3886986/150‑1 (MQ=255)
                                                                                aaaaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTg                                                                 >  1:2536600/1‑150 (MQ=255)
                                                                                  aaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGtt                                                               >  1:3581117/1‑150 (MQ=255)
                                                                                     cTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGc                                                            <  1:660844/150‑1 (MQ=255)
                                                                                      tttGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCt                                                           >  1:1027294/1‑150 (MQ=255)
                                                                                          cTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACg                                                       <  1:3983843/150‑1 (MQ=255)
                                                                                           tGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACgg                                                      >  2:3239750/1‑150 (MQ=255)
                                                                                            gAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGc                                                     <  2:1868444/150‑1 (MQ=255)
                                                                                             aaaCGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCa                                                    >  2:64360/1‑150 (MQ=255)
                                                                                                    aaCCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGcc                                             >  2:271517/1‑150 (MQ=255)
                                                                                                      ccGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAg                                           <  2:4804582/150‑1 (MQ=255)
                                                                                                      ccGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAg                                           >  1:2782189/1‑150 (MQ=255)
                                                                                                        gCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTg                                         <  2:242757/150‑1 (MQ=255)
                                                                                                            gTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAGTCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGAGCt                                     <  1:4807460/150‑1 (MQ=255)
                                                                                                             tGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTg                                    >  1:2285550/1‑150 (MQ=255)
                                                                                                              gAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTgg                                   >  1:3448582/1‑150 (MQ=255)
                                                                                                                aTGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCt                                 <  2:4147241/150‑1 (MQ=255)
                                                                                                                aTGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCt                                 <  2:2011568/150‑1 (MQ=255)
                                                                                                                 tGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTa                                >  1:2732425/1‑150 (MQ=255)
                                                                                                                 tGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTa                                <  2:4706781/150‑1 (MQ=255)
                                                                                                                  ggCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAg                               <  2:4867008/150‑1 (MQ=255)
                                                                                                                     aGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGAc                            <  2:1051457/150‑1 (MQ=255)
                                                                                                                       ggAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCg                          >  2:3424450/1‑150 (MQ=255)
                                                                                                                           aaaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAg                      >  2:2263361/1‑150 (MQ=255)
                                                                                                                            aaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGc                     >  2:4608008/1‑150 (MQ=255)
                                                                                                                              cGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCaa                   <  2:2873240/150‑1 (MQ=255)
                                                                                                                                cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                 >  2:3650520/1‑150 (MQ=255)
                                                                                                                                cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                 >  2:2558634/1‑150 (MQ=255)
                                                                                                                                cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGAGCTGGCTAGGACCGAAAGCAACg                 >  2:1777115/1‑150 (MQ=255)
                                                                                                                                  gCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTa               <  2:1468904/150‑1 (MQ=255)
                                                                                                                                   cGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTAc              <  1:4594793/150‑1 (MQ=255)
                                                                                                                                    gTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTAcc             >  2:3940300/1‑150 (MQ=255)
                                                                                                                                        acaggcacagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGACTCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATgg         >  1:79730/1‑150 (MQ=255)
                                                                                                                                        acaggcacagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATgg         <  2:1753655/150‑1 (MQ=255)
                                                                                                                                            gcacagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAat     <  2:739696/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATC  >  CP009273/397813‑398105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑