Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 421,799 C→A 100% I69I (ATC→ATA)  tgt → tRNA‑guanine transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273421,7990CA99.6% 806.5 / ‑4.3 244I69I (ATC→ATA) tgttRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (110/133);  total (110/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

TCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGA  >  CP009273/421652‑421945
                                                                                                                                                   |                                                                                                                                                  
  gTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGa                                                                                                                                                >  1:4504252/1‑150 (MQ=255)
  gTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGa                                                                                                                                                >  2:3118142/1‑150 (MQ=255)
  gTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGa                                                                                                                                                <  1:2868272/150‑1 (MQ=255)
   tGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGaa                                                                                                                                               <  2:484005/150‑1 (MQ=255)
       gTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTg                                                                                                                                           <  2:606628/150‑1 (MQ=255)
       gTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTg                                                                                                                                           >  1:4694692/1‑150 (MQ=255)
        tAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGc                                                                                                                                          >  2:4144104/1‑150 (MQ=255)
          gTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCAc                                                                                                                                        <  1:1088334/150‑1 (MQ=255)
          gTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCAc                                                                                                                                        <  2:2221770/150‑1 (MQ=255)
           tGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACg                                                                                                                                       <  2:1646167/150‑1 (MQ=255)
              aaaCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCg                                                                                                                                    >  2:1834681/1‑150 (MQ=255)
              aaaCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCg                                                                                                                                    <  2:3772636/150‑1 (MQ=255)
                aCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGAt                                                                                                                                  <  1:1958384/150‑1 (MQ=255)
                 cGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATc                                                                                                                                 >  2:4449990/1‑150 (MQ=255)
                 cGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATc                                                                                                                                 <  2:2769516/150‑1 (MQ=255)
                  gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                                <  1:4717462/150‑1 (MQ=255)
                   ccTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTg                                                                                                                               <  2:553128/150‑1 (MQ=255)
                         tttATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAt                                                                                                                         <  1:262933/150‑1 (MQ=255)
                          ttATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtt                                                                                                                        <  2:578011/150‑1 (MQ=255)
                            aTGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtttt                                                                                                                      >  2:1115755/1‑150 (MQ=255)
                             tGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTa                                                                                                                     <  1:2275278/150‑1 (MQ=255)
                               ccTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATg                                                                                                                   >  1:861501/1‑150 (MQ=255)
                                   ttGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGt                                                                                                               <  2:225154/150‑1 (MQ=255)
                                    tGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTg                                                                                                              >  1:1888997/1‑150 (MQ=255)
                                          cTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAggg                                                                                                        >  1:2002265/1‑150 (MQ=255)
                                          cTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAggg                                                                                                        <  1:5121192/150‑1 (MQ=255)
                                          cTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAggg                                                                                                        >  2:2598715/1‑150 (MQ=255)
                                           tACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAgggg                                                                                                       >  2:3631399/1‑150 (MQ=255)
                                            aCGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGc                                                                                                      >  1:355830/1‑150 (MQ=255)
                                            aCGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGc                                                                                                      <  1:2661247/150‑1 (MQ=255)
                                              ggCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCg                                                                                                    <  2:2551491/150‑1 (MQ=255)
                                                  ccGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATcc                                                                                                <  1:4973009/150‑1 (MQ=255)
                                                   cGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCt                                                                                               <  1:1800081/150‑1 (MQ=255)
                                                    gTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTc                                                                                              <  2:2387942/150‑1 (MQ=255)
                                                    gTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTc                                                                                              <  1:3271711/150‑1 (MQ=255)
                                                     tAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCa                                                                                             >  2:3967212/1‑150 (MQ=255)
                                                         aGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGa                                                                                         <  2:4713437/150‑1 (MQ=255)
                                                         aGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGa                                                                                         >  1:967581/1‑150 (MQ=255)
                                                         aGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGa                                                                                         <  1:615959/150‑1 (MQ=255)
                                                             aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTcc                                                                                     <  1:4398814/150‑1 (MQ=255)
                                                              tGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcg                                                                                    >  1:2944679/1‑150 (MQ=255)
                                                              tGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcg                                                                                    >  1:4447680/1‑150 (MQ=255)
                                                               gACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcgg                                                                                   >  2:1442908/1‑150 (MQ=255)
                                                               gACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcgg                                                                                   >  2:4962196/1‑150 (MQ=255)
                                                                aCGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcggc                                                                                  <  2:3652721/150‑1 (MQ=255)
                                                                 cGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcggcg                                                                                 <  2:1249349/150‑1 (MQ=255)
                                                                    cgtaagaagTTGAAGACACTGGCGCTCAAATTATCCTCGGAAACACCTTCCACCTGTGTCTGCGCCCGGTCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTTTGCAGTGGAAGGGGCCGATCCTCACCGACTCCGgctgct                                                                              <  2:870789/147‑1 (MQ=255)
                                                                     ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                             <  1:4205426/150‑1 (MQ=255)
                                                                       aagaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTcc                                                                           <  1:3481447/150‑1 (MQ=255)
                                                                        agaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCa                                                                          <  2:1900638/150‑1 (MQ=255)
                                                                         gaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAg                                                                         <  1:1108764/150‑1 (MQ=255)
                                                                         gaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAg                                                                         <  2:1768428/150‑1 (MQ=255)
                                                                         gaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCAAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATTCAGTGGAAGTGGCCGATCCTCACCGACTACGGCGGCTTcaat                                                                         >  2:1738615/1‑147 (MQ=255)
                                                                              tGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCtt                                                                    <  2:615296/150‑1 (MQ=255)
                                                                               gAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTc                                                                   <  1:1645366/150‑1 (MQ=255)
                                                                               gAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTc                                                                   <  2:430016/150‑1 (MQ=255)
                                                                                   ccACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                               <  2:3917097/150‑1 (MQ=255)
                                                                                   ccACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                               <  1:1490951/150‑1 (MQ=255)
                                                                                     aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCtt                                                             <  2:2994649/150‑1 (MQ=255)
                                                                                        ggCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGc                                                          <  1:2357037/150‑1 (MQ=255)
                                                                                        ggCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGc                                                          >  1:3205074/1‑150 (MQ=255)
                                                                                           gcgcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGat                                                       <  1:2424898/150‑1 (MQ=255)
                                                                                            ctcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGata                                                      <  2:4398722/148‑1 (MQ=255)
                                                                                            cgcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGata                                                      >  1:3989796/1‑150 (MQ=255)
                                                                                              cAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATAtt                                                    <  1:138489/150‑1 (MQ=255)
                                                                                                        tCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCa                                          >  1:2297918/1‑150 (MQ=255)
                                                                                                        tCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCa                                          <  1:4372496/150‑1 (MQ=255)
                                                                                                            cAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGa                                      <  2:3330669/150‑1 (MQ=255)
                                                                                                             aaCACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGaa                                     >  2:3503650/1‑150 (MQ=255)
                                                                                                              acacCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAAc                                    <  1:2700075/150‑1 (MQ=255)
                                                                                                                 ccTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAgg                                 <  2:5022751/150‑1 (MQ=255)
                                                                                                                      cACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTg                            >  1:1593993/1‑150 (MQ=255)
                                                                                                                      cACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTg                            <  1:3712948/150‑1 (MQ=255)
                                                                                                                       aCCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGc                           <  2:1784966/150‑1 (MQ=255)
                                                                                                                         cTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCAc                         <  1:223597/150‑1 (MQ=255)
                                                                                                                         cTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCAc                         <  1:530294/150‑1 (MQ=255)
                                                                                                                            tgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCCCTTc                      >  1:678838/1‑150 (MQ=255)
                                                                                                                            tgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTc                      >  2:3584555/1‑150 (MQ=255)
                                                                                                                                tGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTa                  >  1:4980445/1‑150 (MQ=255)
                                                                                                                                tGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTa                  >  2:1196005/1‑150 (MQ=255)
                                                                                                                                 gcgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTaa                 >  1:744900/1‑150 (MQ=255)
                                                                                                                                 gcgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTaa                 >  2:4209666/1‑150 (MQ=255)
                                                                                                                                 gcgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTaa                 >  2:276618/1‑150 (MQ=255)
                                                                                                                                   gcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAcc               <  1:2147880/150‑1 (MQ=255)
                                                                                                                                   gcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAcc               >  2:7790/1‑150 (MQ=255)
                                                                                                                                    cccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAccc              <  1:3054280/150‑1 (MQ=255)
                                                                                                                                    cccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAccc              >  2:3629693/1‑150 (MQ=255)
                                                                                                                                    cccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAccc              >  2:2917536/1‑150 (MQ=255)
                                                                                                                                     ccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCg             <  2:4233394/150‑1 (MQ=255)
                                                                                                                                     ccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCg             <  2:2002265/150‑1 (MQ=255)
                                                                                                                                       gggCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGAt           >  2:1878114/1‑150 (MQ=255)
                                                                                                                                       gggCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGAt           >  1:1270385/1‑150 (MQ=255)
                                                                                                                                         gCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCa         <  2:4378598/150‑1 (MQ=255)
                                                                                                                                           cAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAAc       >  2:1573417/1‑150 (MQ=255)
                                                                                                                                            aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACg      <  2:1081616/150‑1 (MQ=255)
                                                                                                                                            aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACg      >  2:316399/1‑150 (MQ=255)
                                                                                                                                              gAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGACCAGGGCGTGCACTTCCGTAACCCGATCAACGGc    >  2:3231785/1‑150 (MQ=255)
                                                                                                                                              gAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGc    <  2:4504252/150‑1 (MQ=255)
                                                                                                                                               aaaTAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCg   >  1:903380/1‑150 (MQ=255)
                                                                                                                                                aataatGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGa  <  2:402676/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGA  >  CP009273/421652‑421945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑