Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 432,762 G→T 100% P277Q (CCG→CAG)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273432,7620GT98.2% 889.5 / 5.0 275P277Q (CCG→CAG) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (138/132);  total (139/136)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

TCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGC  >  CP009273/432615‑432910
                                                                                                                                                   |                                                                                                                                                    
tctTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGt                                                                                                                                                    >  2:1931299/1‑150 (MQ=255)
      ttAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTc                                                                                                                                              <  2:479067/150‑1 (MQ=255)
       tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                             <  2:646109/150‑1 (MQ=255)
        aaaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaa                                                                                                                                            >  1:1807746/1‑150 (MQ=255)
           tCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaacaa                                                                                                                                         <  2:822943/150‑1 (MQ=255)
            ccTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                        >  2:3211390/1‑150 (MQ=255)
                 gACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGc                                                                                                                                   >  1:2928803/1‑150 (MQ=255)
                 gACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGc                                                                                                                                   >  1:3615392/1‑150 (MQ=255)
                    aGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCATTGGCTCATCACGCTGTGCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGGTTACTCAACAACCAGGCCAg                                                                                                                                >  1:3301755/1‑150 (MQ=255)
                    aGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAg                                                                                                                                >  2:1401885/1‑150 (MQ=255)
                     ggATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAgc                                                                                                                               >  1:4722885/1‑150 (MQ=255)
                      gATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAgcg                                                                                                                              <  2:3319796/150‑1 (MQ=255)
                       aTGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAgcgc                                                                                                                             <  2:2238393/150‑1 (MQ=255)
                           ggTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACt                                                                                                                         >  2:3603715/1‑150 (MQ=255)
                               tataCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTtgtg                                                                                                                     >  2:1284432/1‑150 (MQ=255)
                                ataCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGc                                                                                                                    <  1:2842636/150‑1 (MQ=255)
                                    ggCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgt                                                                                                                >  1:2667443/1‑150 (MQ=255)
                                    ggCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgt                                                                                                                <  1:848559/150‑1 (MQ=255)
                                     gCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtg                                                                                                               <  2:3818672/150‑1 (MQ=255)
                                      cGTGTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                              <  2:2919483/150‑1 (MQ=255)
                                       gTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTc                                                                                                             <  2:4607061/150‑1 (MQ=255)
                                       gTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTc                                                                                                             <  1:5093827/150‑1 (MQ=255)
                                       gTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTc                                                                                                             <  2:2344775/150‑1 (MQ=255)
                                        tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCg                                                                                                            >  1:4389803/1‑150 (MQ=255)
                                        tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTAAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCg                                                                                                            >  1:224379/1‑150 (MQ=255)
                                           ccAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCCGCGCAACTTGTGCTCGTGTCGccc                                                                                                         >  2:5108384/1‑150 (MQ=255)
                                           ccAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccc                                                                                                         >  2:3504105/1‑150 (MQ=255)
                                           ccAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccc                                                                                                         >  1:1385441/1‑150 (MQ=255)
                                            cAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGcccc                                                                                                        >  1:2720398/1‑150 (MQ=255)
                                               ttCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg                                                                                                     <  1:4917007/150‑1 (MQ=255)
                                               ttCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg                                                                                                     <  1:2412991/150‑1 (MQ=255)
                                                tCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGt                                                                                                    <  1:4269117/150‑1 (MQ=255)
                                                 cGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGtt                                                                                                   >  2:2369857/1‑150 (MQ=255)
                                                 cGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGtt                                                                                                   <  1:4697868/150‑1 (MQ=255)
                                                    cAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                                <  2:3954748/150‑1 (MQ=255)
                                                    cAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                                >  2:2185424/1‑150 (MQ=255)
                                                     aaTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttcttc                                                                                               >  1:557852/1‑150 (MQ=255)
                                                      aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                              >  2:1200368/1‑150 (MQ=255)
                                                        cTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACt                                                                                            <  2:1693578/150‑1 (MQ=255)
                                                         tGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTg                                                                                           >  1:3586131/1‑150 (MQ=255)
                                                           ttCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGAc                                                                                         <  2:4900400/150‑1 (MQ=255)
                                                            tCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACg                                                                                        >  2:3426107/1‑150 (MQ=255)
                                                              cGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGcc                                                                                      >  1:3699703/1‑150 (MQ=255)
                                                                 cTTCATAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGt                                                                                   <  2:2674763/150‑1 (MQ=255)
                                                                   tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTa                                                                                 <  2:1769925/150‑1 (MQ=255)
                                                                    cAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTaa                                                                                >  2:4645703/1‑150 (MQ=255)
                                                                     aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                               >  1:522694/1‑150 (MQ=255)
                                                                     aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                               <  2:43835/150‑1 (MQ=255)
                                                                     aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                               <  1:1411949/150‑1 (MQ=255)
                                                                         tGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGATTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGGTCTTCACTGACGCCTGTTAACCGct                                                                           >  2:4275405/1‑150 (MQ=255)
                                                                          gATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctc                                                                          >  1:2696226/1‑150 (MQ=255)
                                                                             atCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGc                                                                       >  1:4452697/1‑150 (MQ=255)
                                                                             atCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGc                                                                       <  1:2321872/150‑1 (MQ=255)
                                                                              tCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCg                                                                      >  1:585432/1‑150 (MQ=255)
                                                                                taCCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGAt                                                                    >  2:4726219/2‑150 (MQ=255)
                                                                                cACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGAt                                                                    <  2:755364/150‑1 (MQ=255)
                                                                                  ccGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCt                                                                  >  2:877460/1‑150 (MQ=255)
                                                                                        tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGt                                                            >  2:4106541/1‑150 (MQ=255)
                                                                                         cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                           >  1:1132928/1‑150 (MQ=255)
                                                                                         cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                           >  2:1102871/1‑150 (MQ=255)
                                                                                         cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                           >  1:1060866/1‑150 (MQ=255)
                                                                                         cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                           >  1:3066589/1‑150 (MQ=255)
                                                                                         cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                           >  1:951477/1‑150 (MQ=255)
                                                                                          aaTAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa                                                          >  2:4184877/1‑150 (MQ=255)
                                                                                              gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtttt                                                      <  2:1903873/150‑1 (MQ=255)
                                                                                              gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtttt                                                      <  1:4141746/150‑1 (MQ=255)
                                                                                                tcatcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                    >  1:935820/1‑150 (MQ=255)
                                                                                                  atcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATc                                                  >  1:1149567/1‑150 (MQ=255)
                                                                                                     aaGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                               >  1:453390/1‑150 (MQ=255)
                                                                                                      aGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctt                                              <  1:71364/150‑1 (MQ=255)
                                                                                                       gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGcttt                                             <  1:406513/150‑1 (MQ=255)
                                                                                                         tGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                           >  1:494395/1‑150 (MQ=255)
                                                                                                            tcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTta                                        <  2:253430/150‑1 (MQ=255)
                                                                                                             cttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtat                                       <  1:3519840/150‑1 (MQ=255)
                                                                                                             cttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtat                                       <  1:375400/150‑1 (MQ=255)
                                                                                                               tcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATag                                     <  1:125027/150‑1 (MQ=255)
                                                                                                                cgcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATaga                                    >  1:199567/1‑150 (MQ=255)
                                                                                                                 gcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATagag                                   >  1:2746332/1‑150 (MQ=255)
                                                                                                                     gAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttt                               <  1:3140214/150‑1 (MQ=255)
                                                                                                                      aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtttt                              <  2:1967833/150‑1 (MQ=255)
                                                                                                                      aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtttt                              >  1:582935/1‑150 (MQ=255)
                                                                                                                          tCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTccc                          >  2:2808218/1‑150 (MQ=255)
                                                                                                                           ccGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcccc                         <  1:5043412/150‑1 (MQ=255)
                                                                                                                            cGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCa                        <  1:87653/150‑1 (MQ=255)
                                                                                                                                 aTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtca                   <  2:2574930/150‑1 (MQ=255)
                                                                                                                                 aTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtca                   >  1:3228360/1‑150 (MQ=255)
                                                                                                                                 aTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtca                   <  2:5063445/150‑1 (MQ=255)
                                                                                                                                  tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtcat                  <  1:4179814/150‑1 (MQ=255)
                                                                                                                                  tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtcat                  <  1:469097/150‑1 (MQ=255)
                                                                                                                                     ctgaggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCAGTTAACCGCACTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAg               >  2:1069007/5‑150 (MQ=255)
                                                                                                                                        tggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGaca            >  1:3965749/2‑150 (MQ=255)
                                                                                                                                         ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac           >  2:492997/1‑150 (MQ=255)
                                                                                                                                         ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac           >  2:2282555/1‑150 (MQ=255)
                                                                                                                                         ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac           <  2:4697071/150‑1 (MQ=255)
                                                                                                                                         ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac           <  2:2442217/150‑1 (MQ=255)
                                                                                                                                          gCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACAcc          >  1:2094596/1‑150 (MQ=255)
                                                                                                                                          gCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACAcc          <  2:3871832/150‑1 (MQ=255)
                                                                                                                                          gCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACAcc          >  2:3677384/1‑150 (MQ=255)
                                                                                                                                           cAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCa         >  1:3914572/1‑150 (MQ=255)
                                                                                                                                               gCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCACCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCg     <  2:296876/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGC  >  CP009273/432615‑432910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑