Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 444,489 G→A 100% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,4890GA99.5% 1046.9 / ‑4.5 313R537C (CGT→TGT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (151/160);  total (152/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 313 total aligned reads displayed.

GCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGT  >  CP009273/444342‑444635
                                                                                                                                                   |                                                                                                                                                  
gCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGc                                                                                                                                                  <  1:535104/150‑1 (MQ=255)
 cATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGcc                                                                                                                                                 >  1:3700305/1‑150 (MQ=255)
 cATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGcc                                                                                                                                                 <  2:4336839/150‑1 (MQ=255)
       aaTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                           >  1:2061878/1‑150 (MQ=255)
       aaTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                           <  1:2029623/150‑1 (MQ=255)
         tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACg                                                                                                                                         >  1:1173733/1‑150 (MQ=255)
           tcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACggg                                                                                                                                       <  1:880233/150‑1 (MQ=255)
             ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGtt                                                                                                                                     <  1:4435374/150‑2 (MQ=255)
               cATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGc                                                                                                                                   >  1:3458632/1‑150 (MQ=255)
                aTAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGcc                                                                                                                                  >  1:246513/1‑150 (MQ=255)
                   gTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGt                                                                                                                               >  2:2244846/1‑150 (MQ=255)
                    tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                              >  2:646890/1‑150 (MQ=255)
                    tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                              >  1:3192245/1‑150 (MQ=255)
                    tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                              >  1:2478860/1‑150 (MQ=255)
                     ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCa                                                                                                                             >  2:2817413/1‑150 (MQ=255)
                     ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCa                                                                                                                             <  2:4307274/150‑1 (MQ=255)
                      gTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                            <  2:1434726/150‑1 (MQ=255)
                        cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGt                                                                                                                          >  1:447621/1‑150 (MQ=255)
                         aGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTc                                                                                                                         <  1:3036543/150‑1 (MQ=255)
                          ggCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCa                                                                                                                        >  1:4058533/1‑150 (MQ=255)
                          ggCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCa                                                                                                                        >  2:4670090/1‑150 (MQ=255)
                               tttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGACGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                                                                                   >  2:3011717/1‑150 (MQ=255)
                               tttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                                                                                   <  1:2546842/150‑1 (MQ=255)
                                   taCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTgg                                                                                                               >  2:3205527/1‑150 (MQ=255)
                                   taCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTgg                                                                                                               >  1:4473243/1‑150 (MQ=255)
                                     cGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtc                                                                                                             >  1:5049502/1‑150 (MQ=255)
                                      gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                                                                                            <  2:1081308/150‑1 (MQ=255)
                                      gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                                                                                            >  2:1922829/1‑150 (MQ=255)
                                      gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                                                                                            >  2:2905286/1‑150 (MQ=255)
                                      gCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                                                                                            >  2:4493662/1‑150 (MQ=255)
                                       cTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                           >  1:1453465/1‑150 (MQ=255)
                                        ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcg                                                                                                          >  1:5098731/1‑150 (MQ=255)
                                            ccTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcg                                                                                                      <  2:131241/150‑1 (MQ=255)
                                              tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                                    >  2:817334/1‑150 (MQ=255)
                                                   ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                                                                                               <  1:2505398/150‑1 (MQ=255)
                                                    tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGa                                                                                              <  2:623835/150‑1 (MQ=255)
                                                     ttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaa                                                                                             <  1:2306092/150‑1 (MQ=255)
                                                       cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                           <  1:990443/150‑1 (MQ=255)
                                                       cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                           <  1:1298432/150‑1 (MQ=255)
                                                          ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTa                                                                                        <  1:4490113/150‑1 (MQ=255)
                                                           tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                       <  2:3429152/150‑1 (MQ=255)
                                                           tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                       <  1:3062594/150‑1 (MQ=255)
                                                             ccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                     <  1:1371809/150‑1 (MQ=255)
                                                              cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                                                    >  1:4627712/1‑150 (MQ=255)
                                                               aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  1:265233/1‑150 (MQ=255)
                                                               aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  2:2545912/1‑150 (MQ=255)
                                                               aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  1:1094814/1‑150 (MQ=255)
                                                                gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTg                                                                                  >  2:5101783/1‑150 (MQ=255)
                                                                gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTg                                                                                  <  1:2017457/150‑1 (MQ=255)
                                                                gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATATg                                                                                  <  2:4169124/150‑1 (MQ=255)
                                                                    gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                              <  1:297654/150‑1 (MQ=255)
                                                                       tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACg                                                                           <  1:4592629/150‑1 (MQ=255)
                                                                        cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATACCGa                                                                          >  2:3578295/1‑150 (MQ=255)
                                                                        cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                          >  2:3954955/1‑150 (MQ=255)
                                                                        cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                          >  1:1598290/1‑150 (MQ=255)
                                                                          cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg                                                                        <  1:4004197/150‑1 (MQ=255)
                                                                           gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                       <  1:669009/150‑1 (MQ=255)
                                                                           gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                       >  2:3514141/1‑150 (MQ=255)
                                                                           gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                       >  1:3085879/1‑150 (MQ=255)
                                                                             tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCag                                                                     <  1:1011704/150‑1 (MQ=255)
                                                                              cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga                                                                    >  1:1921491/1‑150 (MQ=255)
                                                                              cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga                                                                    <  2:2187201/150‑1 (MQ=255)
                                                                              cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga                                                                    <  1:2935076/150‑1 (MQ=255)
                                                                              cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGACAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga                                                                    >  1:1807845/1‑150 (MQ=255)
                                                                               gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCagag                                                                   <  1:3347160/150‑1 (MQ=255)
                                                                               gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCagag                                                                   >  2:3857007/1‑150 (MQ=255)
                                                                               gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCagag                                                                   <  1:766130/150‑1 (MQ=255)
                                                                                 gAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGACGCAGAGaa                                                                 >  1:216843/1‑150 (MQ=255)
                                                                                    cGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCTCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATAc                                                              <  1:4293413/150‑1 (MQ=255)
                                                                                       gCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCa                                                           >  2:732235/1‑150 (MQ=255)
                                                                                        cGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAg                                                          >  2:4551276/1‑150 (MQ=255)
                                                                                        cGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAg                                                          >  2:268891/1‑150 (MQ=255)
                                                                                           cACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcgc                                                       >  1:1234546/1‑150 (MQ=255)
                                                                                            aCTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCa                                                      >  1:2818494/1‑150 (MQ=255)
                                                                                             cTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCaa                                                     <  1:3276074/150‑1 (MQ=255)
                                                                                                   cAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTa                                               >  1:4174260/1‑150 (MQ=255)
                                                                                                     aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACt                                             >  2:4156861/1‑150 (MQ=255)
                                                                                                        ttATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCa                                          <  2:3686887/150‑1 (MQ=255)
                                                                                                         tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                         <  2:442085/150‑1 (MQ=255)
                                                                                                                  gAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggctg                                <  2:515165/150‑1 (MQ=255)
                                                                                                                   aGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGc                               >  1:1849129/1‑150 (MQ=255)
                                                                                                                    ggCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCa                              <  2:2979486/150‑1 (MQ=255)
                                                                                                                      tggggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAat                            >  2:3189875/2‑150 (MQ=255)
                                                                                                                           aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                       >  1:3920627/1‑150 (MQ=255)
                                                                                                                           aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                       >  2:1362635/1‑150 (MQ=255)
                                                                                                                            agagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAg                      >  2:2051189/1‑150 (MQ=255)
                                                                                                                             gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGc                     >  2:3712702/1‑150 (MQ=255)
                                                                                                                              agGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa                    <  1:4931660/150‑1 (MQ=255)
                                                                                                                              agGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa                    <  2:128124/150‑1 (MQ=255)
                                                                                                                              agGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa                    >  2:1622165/1‑150 (MQ=255)
                                                                                                                               ggTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                   <  1:566690/150‑1 (MQ=255)
                                                                                                                               ggTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                   <  1:403528/150‑1 (MQ=255)
                                                                                                                                  tGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt                <  1:4804020/150‑1 (MQ=255)
                                                                                                                                    cccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgtgt              >  1:1752792/1‑150 (MQ=255)
                                                                                                                                         tCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAAc         >  2:4900850/1‑150 (MQ=255)
                                                                                                                                          ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACt        <  2:2478860/150‑1 (MQ=255)
                                                                                                                                           cAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg       <  2:3995273/150‑1 (MQ=255)
                                                                                                                                           cAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg       <  2:196028/150‑1 (MQ=255)
                                                                                                                                               gTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCggg   >  1:3611358/1‑150 (MQ=255)
                                                                                                                                                tACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGt  >  1:1406444/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGT  >  CP009273/444342‑444635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑