Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 40,957 C→A 100% V325V (GTG→GTT)  caiT ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927340,9570CA98.0% 959.4 / 6.4 299V325V (GTG→GTT) caiTputative transporter
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (150/142);  total (153/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 326 total aligned reads displayed.

TATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGCT  >  CP009273/40810‑41103
                                                                                                                                                   |                                                                                                                                                  
tatcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaac                                                                                                                                                  <  2:783193/150‑1 (MQ=255)
 atcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacc                                                                                                                                                 >  1:3783809/1‑150 (MQ=255)
 atcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacc                                                                                                                                                 <  1:1116836/150‑1 (MQ=255)
  tcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccc                                                                                                                                                <  2:3527707/150‑1 (MQ=255)
    aaCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacccac                                                                                                                                              >  2:1855848/1‑150 (MQ=255)
       agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                           <  1:5150976/150‑1 (MQ=255)
       agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                           <  2:3799885/150‑1 (MQ=255)
        gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATCAATAACCCACCATg                                                                                                                                          >  2:498527/1‑150 (MQ=255)
        gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATg                                                                                                                                          >  2:3725711/1‑150 (MQ=255)
          agagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                        >  1:15632/1‑150 (MQ=255)
          agagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                        <  2:858954/150‑1 (MQ=255)
          agagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                        >  1:2595827/1‑150 (MQ=255)
           gagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGccc                                                                                                                                       <  1:3954217/150‑1 (MQ=255)
            agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCa                                                                                                                                      <  1:273606/150‑1 (MQ=255)
               gtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTa                                                                                                                                   >  2:4424663/1‑150 (MQ=255)
                       cGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACgg                                                                                                                           >  1:4401391/1‑150 (MQ=255)
                         agtaacgTCCACAGGATCCAGGTTGACGCTGTCAGCCCAATCACCATTCCGAAGAACAGTTCACGCACAGTACGACAACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTATAACACGGTc                                                                                                                         >  1:23461/7‑150 (MQ=255)
                          gTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTcc                                                                                                                        <  1:1930519/150‑1 (MQ=255)
                           tACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCa                                                                                                                       >  1:2399315/1‑150 (MQ=255)
                                tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCt                                                                                                                  <  1:2186048/150‑1 (MQ=255)
                                 ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTg                                                                                                                 <  2:1143304/150‑1 (MQ=255)
                                 ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTg                                                                                                                 <  1:745339/150‑1 (MQ=255)
                                 ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTg                                                                                                                 <  1:30232/150‑1 (MQ=255)
                                   acaGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCg                                                                                                               >  1:3838627/1‑150 (MQ=255)
                                    caGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCgg                                                                                                              <  1:1169780/150‑1 (MQ=255)
                                    caGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCgg                                                                                                              >  2:2169310/1‑150 (MQ=255)
                                      ggATCCAGGTTGACGCTGTCAGCCCCAGCTCCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGa                                                                                                            <  2:3365589/150‑1 (MQ=255)
                                       gATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGaa                                                                                                           >  1:4787031/1‑150 (MQ=255)
                                       gATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGaa                                                                                                           >  1:3558216/1‑150 (MQ=255)
                                          ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgcc                                                                                                        <  1:871857/150‑1 (MQ=255)
                                            aGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                      >  2:2856205/1‑150 (MQ=255)
                                              gTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCt                                                                                                    >  2:795708/1‑150 (MQ=255)
                                                tGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCttt                                                                                                  >  1:1875241/1‑150 (MQ=255)
                                                 gACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTa                                                                                                 <  1:1223586/150‑1 (MQ=255)
                                                   cGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc                                                                                               <  1:1708142/150‑1 (MQ=255)
                                                   cGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGc                                                                                               <  1:4996093/150‑1 (MQ=255)
                                                    gCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCg                                                                                              <  2:2775018/150‑1 (MQ=255)
                                                       gTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATg                                                                                           >  2:3937222/1‑150 (MQ=255)
                                                       gTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATg                                                                                           <  2:4259501/150‑1 (MQ=255)
                                                        tCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg                                                                                          <  1:719989/150‑1 (MQ=255)
                                                        tCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg                                                                                          >  1:1445710/1‑150 (MQ=255)
                                                        tCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATgg                                                                                          >  2:223555/1‑150 (MQ=255)
                                                          aGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                        <  1:481714/150‑1 (MQ=255)
                                                             cccAGCCCCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCAGAAATAACCCACCCTGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                     <  2:4156692/150‑1 (MQ=255)
                                                             cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                     <  2:3261549/150‑1 (MQ=255)
                                                             cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                     >  1:4304792/1‑150 (MQ=255)
                                                             cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                     >  1:399910/1‑150 (MQ=255)
                                                                aGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGta                                                                                  <  2:2284127/150‑1 (MQ=255)
                                                                   aCCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAg                                                                               >  1:3849812/1‑150 (MQ=255)
                                                                     cATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaa                                                                             >  2:4964216/1‑150 (MQ=255)
                                                                      aTGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaac                                                                            <  1:59345/150‑1 (MQ=255)
                                                                       tGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaaca                                                                           >  2:1525222/1‑150 (MQ=255)
                                                                         ccGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaac                                                                         <  1:521556/150‑1 (MQ=255)
                                                                          cGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                        <  2:327436/150‑1 (MQ=255)
                                                                            aaGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATg                                                                      <  1:114660/150‑1 (MQ=255)
                                                                             aGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgc                                                                     >  1:3322944/1‑150 (MQ=255)
                                                                               cacaGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcgc                                                                   <  2:1061419/150‑1 (MQ=255)
                                                                               cacaGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcgc                                                                   <  2:4372270/150‑1 (MQ=255)
                                                                                 caGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCgg                                                                 <  2:3902811/150‑1 (MQ=255)
                                                                                 caGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCgg                                                                 >  1:2083044/1‑150 (MQ=255)
                                                                                  aGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGc                                                                >  1:4456628/1‑150 (MQ=255)
                                                                                  aGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGc                                                                <  2:2559755/150‑1 (MQ=255)
                                                                                    ttCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTCTAGAACAACATGCGCGGCAg                                                              >  2:2997618/1‑150 (MQ=255)
                                                                                      cACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGAt                                                            <  2:3658126/150‑1 (MQ=255)
                                                                                        cGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATAc                                                          <  1:3091832/150‑1 (MQ=255)
                                                                                        cGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATAc                                                          >  1:1880921/1‑150 (MQ=255)
                                                                                             aGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAg                                                     <  2:247117/150‑1 (MQ=255)
                                                                                             aGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAg                                                     <  2:269839/150‑1 (MQ=255)
                                                                                                   aCCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACAt                                               <  2:1224671/150‑1 (MQ=255)
                                                                                                     cACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcc                                             >  2:4907886/1‑150 (MQ=255)
                                                                                                       cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGTTACATCAGCAACATcccc                                           <  2:4718699/150‑1 (MQ=255)
                                                                                                       cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcccc                                           >  1:4133207/1‑150 (MQ=255)
                                                                                                       cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcccc                                           <  2:1724370/150‑1 (MQ=255)
                                                                                                        gggAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCa                                          >  2:5140092/1‑150 (MQ=255)
                                                                                                          gagaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCAcc                                        >  2:4214995/1‑150 (MQ=255)
                                                                                                          gagaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCAcc                                        <  2:4848979/150‑1 (MQ=255)
                                                                                                          gagaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCAcc                                        <  1:2856146/150‑1 (MQ=255)
                                                                                                              tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                    >  2:719743/1‑150 (MQ=255)
                                                                                                                     gAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                             >  1:1670655/1‑150 (MQ=255)
                                                                                                                     gAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGaa                             >  1:3811251/1‑150 (MQ=255)
                                                                                                                      aGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAag                            >  2:1990542/1‑150 (MQ=255)
                                                                                                                       ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagt                           >  2:938490/1‑150 (MQ=255)
                                                                                                                            aTACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                      >  2:2378400/1‑150 (MQ=255)
                                                                                                                              aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                    >  2:588759/1‑150 (MQ=255)
                                                                                                                                 cATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgat                 <  1:3527692/150‑1 (MQ=255)
                                                                                                                                  aTCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgata                <  1:4625213/150‑2 (MQ=255)
                                                                                                                                   tCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCAtgatga               <  2:4642053/150‑1 (MQ=255)
                                                                                                                                   tCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCAtgatga               >  2:2359003/1‑150 (MQ=255)
                                                                                                                                    cTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGaa              >  2:1796700/1‑150 (MQ=255)
                                                                                                                                    cTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGaa              >  1:1380627/1‑150 (MQ=255)
                                                                                                                                     tGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAg             <  1:3260210/150‑1 (MQ=255)
                                                                                                                                       gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCt           >  2:642933/1‑150 (MQ=255)
                                                                                                                                       gATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCt           >  1:2881453/1‑150 (MQ=255)
                                                                                                                                        aTAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTg          >  2:1308476/1‑150 (MQ=255)
                                                                                                                                           gCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCa       <  1:3937222/150‑1 (MQ=255)
                                                                                                                                            cATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAc      >  1:541966/1‑150 (MQ=255)
                                                                                                                                            cATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAc      <  2:2818625/150‑1 (MQ=255)
                                                                                                                                             ataaataaCCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAcc     <  1:5040316/150‑1 (MQ=255)
                                                                                                                                             ataaataaCCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCAcc     >  1:4276551/1‑150 (MQ=255)
                                                                                                                                              tAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCg    <  2:529952/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGCT  >  CP009273/40810‑41103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑