Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 449,423 G→T 18.4% I67I (ATC→ATA)  yajG ← putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273449,4230GT18.4% 795.7 / 115.7 292I67I (ATC→ATA) yajGputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (128/110);  new base T (28/26);  total (156/136)
Fisher's exact test for biased strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCG  >  CP009273/449274‑449570
                                                                                                                                                     |                                                                                                                                                   
cccTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCAAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACg                                                                                                                                                     >  2:4790478/1‑150 (MQ=255)
        cacGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGAtt                                                                                                                                             >  1:5144843/1‑150 (MQ=255)
          cGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTAt                                                                                                                                           <  2:1477387/150‑1 (MQ=255)
           gTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATc                                                                                                                                          >  1:789323/1‑150 (MQ=255)
             cAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATcgc                                                                                                                                        <  2:122884/150‑1 (MQ=255)
              aGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATcgcg                                                                                                                                       >  2:3889645/1‑150 (MQ=255)
                cATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCggg                                                                                                                                     >  1:1841725/1‑150 (MQ=255)
                cATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCggg                                                                                                                                     >  1:481498/1‑150 (MQ=255)
                cATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGAACGCGGGAGGCGGTCAGGGTAACGATTTGATAATctctgg                                                                                                                                     >  2:4319459/1‑145 (MQ=255)
                   aCAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGa                                                                                                                                  >  2:21499/1‑150 (MQ=255)
                       ttGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCCGGGTAACGATTTGATTATCGCGGGTGACttt                                                                                                                              >  2:1746688/1‑150 (MQ=255)
                       ttGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACttt                                                                                                                              >  2:2408985/1‑150 (MQ=255)
                               cGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAgcg                                                                                                                      >  1:2856614/1‑150 (MQ=255)
                                gatgatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAgcgc                                                                                                                     >  2:4852580/1‑150 (MQ=255)
                                  tgatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCt                                                                                                                   <  1:3898537/150‑1 (MQ=255)
                                   gatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTg                                                                                                                  <  2:4393051/150‑1 (MQ=255)
                                    atTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGa                                                                                                                 <  1:3358340/150‑1 (MQ=255)
                                      ttGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATc                                                                                                               <  1:1330813/150‑1 (MQ=255)
                                       tGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCg                                                                                                              <  2:3278938/150‑1 (MQ=255)
                                         cAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGt                                                                                                            <  1:587473/150‑1 (MQ=255)
                                          aGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTa                                                                                                           <  2:2076364/150‑1 (MQ=255)
                                           gATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTAc                                                                                                          <  2:820009/150‑1 (MQ=255)
                                            aTTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACg                                                                                                         <  2:4449758/150‑1 (MQ=255)
                                              tAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCt                                                                                                       >  1:1222163/1‑150 (MQ=255)
                                              tAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCt                                                                                                       <  1:1052626/150‑1 (MQ=255)
                                               aaCCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTg                                                                                                      >  1:3714933/1‑150 (MQ=255)
                                                 ccGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGAt                                                                                                    >  2:2704910/1‑150 (MQ=255)
                                                 ccGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGAt                                                                                                    >  2:2704962/1‑150 (MQ=255)
                                                 ccGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGAt                                                                                                    >  1:691492/1‑150 (MQ=255)
                                                  cGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATc                                                                                                   <  2:4912749/150‑1 (MQ=255)
                                                  cGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATc                                                                                                   <  1:2689635/150‑1 (MQ=255)
                                                  cGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATc                                                                                                   <  1:2683820/150‑1 (MQ=255)
                                                   gggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCg                                                                                                  <  1:3444305/150‑1 (MQ=255)
                                                   gggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCg                                                                                                  >  2:2658984/1‑150 (MQ=255)
                                                   gggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCg                                                                                                  <  2:3549385/150‑1 (MQ=255)
                                                   gggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCg                                                                                                  >  2:2256114/1‑150 (MQ=255)
                                                   gggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCg                                                                                                  <  1:2911264/150‑1 (MQ=255)
                                                      ccATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCa                                                                                               <  1:316096/150‑1 (MQ=255)
                                                          tCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCAt                                                                                           >  2:4080254/1‑150 (MQ=255)
                                                          tCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCAt                                                                                           >  2:4038039/1‑150 (MQ=255)
                                                            ggACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTa                                                                                         >  1:1473310/1‑150 (MQ=255)
                                                             gACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTaa                                                                                        >  1:3076783/1‑150 (MQ=255)
                                                              accaaccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAAt                                                                                       <  1:3516616/150‑1 (MQ=255)
                                                                caaccaTGTAACCACGCGCGGTCATCTGTTTTTCCATCACTTCTTGCAGCAGGAAACGCAGATCTCGGGAGGCGGTCAGGGTAACGATTTGATTATCTCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGc                                                                                     >  1:79588/1‑150 (MQ=255)
                                                                caaccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGc                                                                                     >  1:2003252/1‑150 (MQ=255)
                                                                 aaccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCt                                                                                    >  2:4165061/1‑150 (MQ=255)
                                                                       gTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGt                                                                              >  2:29719/1‑150 (MQ=255)
                                                                          aCCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGAc                                                                           <  2:1538473/150‑1 (MQ=255)
                                                                           ccACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACg                                                                          >  1:4566496/1‑150 (MQ=255)
                                                                            cACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGc                                                                         >  2:3245132/1‑150 (MQ=255)
                                                                            cACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGc                                                                         >  1:1571652/1‑150 (MQ=255)
                                                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGATACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGccc                                                                       <  2:1036821/150‑1 (MQ=255)
                                                                               gcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCa                                                                      <  2:4630623/150‑1 (MQ=255)
                                                                                 gcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATc                                                                    <  1:960856/150‑1 (MQ=255)
                                                                                  cgGTCATCTGTTTTTCCAGCACTTCTTTCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCa                                                                   >  1:3239705/1‑150 (MQ=255)
                                                                                  cgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCa                                                                   <  2:513692/150‑1 (MQ=255)
                                                                                   ggTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAg                                                                  >  1:4693997/1‑150 (MQ=255)
                                                                                   ggTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAg                                                                  <  2:39117/150‑1 (MQ=255)
                                                                                    gTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAgg                                                                 >  1:1289908/1‑150 (MQ=255)
                                                                                    gTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAgg                                                                 <  1:3605082/150‑1 (MQ=255)
                                                                                       aTCTGTTTTTCCAGCACTTCTTGCAGCAGGATACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCtt                                                              <  1:4927800/150‑1 (MQ=255)
                                                                                          tGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGa                                                           >  1:4047572/1‑150 (MQ=255)
                                                                                          tGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGa                                                           <  2:2781094/150‑1 (MQ=255)
                                                                                           gTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGAt                                                          <  1:1568038/150‑1 (MQ=255)
                                                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATc                                                         >  2:363156/1‑150 (MQ=255)
                                                                                               ttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCctg                                                      <  1:4662502/150‑1 (MQ=255)
                                                                                                   aGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCtgt                                                  <  1:4216554/150‑1 (MQ=255)
                                                                                                     cACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTgg                                                >  1:139462/1‑150 (MQ=255)
                                                                                                     cACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTgg                                                >  2:2204518/1‑150 (MQ=255)
                                                                                                         tcttGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                            <  2:4953053/150‑1 (MQ=255)
                                                                                                          cttGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgtg                                           >  2:4998079/1‑150 (MQ=255)
                                                                                                            tGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTa                                         <  1:250550/150‑1 (MQ=255)
                                                                                                            tGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTa                                         >  2:1917948/1‑150 (MQ=255)
                                                                                                            tGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTGGGATCCTGCTGTGGCAgtgtc                                         >  2:2616569/1‑149 (MQ=255)
                                                                                                              cagcagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAt                                       >  1:226773/1‑150 (MQ=255)
                                                                                                              cagcagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAt                                       >  1:2148914/1‑150 (MQ=255)
                                                                                                               agcagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtc                                      <  1:286857/150‑1 (MQ=255)
                                                                                                               agcagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtc                                      >  1:882362/1‑150 (MQ=255)
                                                                                                                 cagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcgt                                    <  2:62432/150‑1 (MQ=255)
                                                                                                                  agGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcgtc                                   <  1:1721569/150‑1 (MQ=255)
                                                                                                                   ggAAACGCAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcgtcg                                  >  1:2182822/1‑150 (MQ=255)
                                                                                                                   ggAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcgtcg                                  >  1:3287544/1‑150 (MQ=255)
                                                                                                                          cAGATCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAAc                           >  1:3243878/1‑150 (MQ=255)
                                                                                                                           aGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGTGGAAACt                          >  1:1125780/1‑150 (MQ=255)
                                                                                                                            gATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACtt                         >  1:629650/1‑150 (MQ=255)
                                                                                                                             aTCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTc                        <  1:921789/150‑1 (MQ=255)
                                                                                                                              tCGCGGGAGGCGGTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCa                       <  2:2313294/150‑1 (MQ=255)
                                                                                                                                       gCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAgttgtt              >  2:1803557/1‑150 (MQ=255)
                                                                                                                                         ggTCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTgg            <  2:4717895/150‑1 (MQ=255)
                                                                                                                                          gTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGc           <  1:4118053/150‑1 (MQ=255)
                                                                                                                                          gTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGc           <  2:722902/150‑1 (MQ=255)
                                                                                                                                           tCAGGGTAACTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCg          <  2:1620517/150‑1 (MQ=255)
                                                                                                                                             aGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGt        >  1:2429414/1‑150 (MQ=255)
                                                                                                                                              gggTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGtt       <  2:5066838/150‑1 (MQ=255)
                                                                                                                                               ggTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGttt      <  2:378834/150‑1 (MQ=255)
                                                                                                                                               ggTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGttt      <  1:392154/150‑1 (MQ=255)
                                                                                                                                                gTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGtttt     <  1:4846027/150‑1 (MQ=255)
                                                                                                                                                  aaCTATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTgc   <  1:165950/150‑1 (MQ=255)
                                                                                                                                                  aaCGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTgc   >  2:3169586/1‑150 (MQ=255)
                                                                                                                                                   aCGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTgag  >  1:3026787/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCG  >  CP009273/449274‑449570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑