Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 969.3 / ‑6.7 294intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (139/150);  minor base T (0/3);  total (140/154)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 294 total aligned reads displayed.

CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450144‑450435
                                                                                                                                                  |                                                                                                                                                 
cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                <  2:523273/150‑1 (MQ=255)
 aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                               >  2:2820486/1‑150 (MQ=255)
  tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                              >  2:1132468/1‑150 (MQ=255)
   gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTa                                                                                                                                             <  1:2624509/150‑1 (MQ=255)
         gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                       <  1:2707418/150‑1 (MQ=255)
          ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                      >  1:1002595/1‑150 (MQ=255)
          ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                      >  2:432743/1‑150 (MQ=255)
          ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                      >  1:1809093/1‑150 (MQ=255)
          ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATATGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATATTATGaaaaa                                                                                                                                      >  2:2666595/1‑150 (MQ=255)
            ttGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                    >  1:3808721/2‑150 (MQ=255)
            ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                    <  1:4945486/150‑1 (MQ=255)
             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                                   >  1:3877186/1‑150 (MQ=255)
             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                                   <  2:699542/150‑1 (MQ=255)
             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                                   >  1:1784689/1‑150 (MQ=255)
              gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                  <  1:2119448/150‑1 (MQ=255)
               aaTACTTACACTATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                 <  1:3960282/149‑1 (MQ=255)
                aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                <  1:3425424/150‑1 (MQ=255)
                 tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTg                                                                                                                               <  1:4486280/150‑1 (MQ=255)
                   cTTACACTATTCAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                             <  2:3290660/150‑1 (MQ=255)
                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                     <  1:2300553/150‑1 (MQ=255)
                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                     <  1:1297830/150‑1 (MQ=255)
                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                     <  2:3184890/150‑1 (MQ=255)
                            ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                                                    <  1:2534650/150‑1 (MQ=255)
                            ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                                                    >  2:533838/1‑150 (MQ=255)
                                   gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                             <  2:3952133/150‑1 (MQ=255)
                                    tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATGTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                            >  2:2501221/1‑150 (MQ=255)
                                     gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                           <  1:3633476/150‑1 (MQ=255)
                                      ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTTCTg                                                                                                          >  1:1745019/1‑150 (MQ=255)
                                        aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGa                                                                                                        >  2:3037383/1‑150 (MQ=255)
                                           ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAttt                                                                                                     <  2:4988988/150‑1 (MQ=255)
                                            gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                    >  2:912791/1‑150 (MQ=255)
                                               gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                 >  2:1342636/1‑150 (MQ=255)
                                               gCAGGACACCGTCTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                 <  1:980103/150‑1 (MQ=255)
                                                cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcgc                                                                                                >  2:2309490/1‑150 (MQ=255)
                                                   gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                             >  2:630667/1‑150 (MQ=255)
                                                   gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                             <  1:113637/150‑1 (MQ=255)
                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                            <  2:3148630/150‑1 (MQ=255)
                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTCAGGAGTTTGAGCtttt                                                                                            >  1:3289928/1‑150 (MQ=255)
                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCtttt                                                                                            >  1:703825/1‑150 (MQ=255)
                                                      acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                          <  1:4427709/150‑1 (MQ=255)
                                                          tCTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAACAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCa                                                                                      <  2:2955506/150‑1 (MQ=255)
                                                              tGCCTCTCCTCCCTGTCGTGGAGCAGGAATCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTATTCCAGTATGTTGCTAAAAATATTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGACAGGATTTTTAGCTATTTTCCCAGCaa                                                                                  >  2:3689120/1‑149 (MQ=255)
                                                               gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                 <  1:1114934/150‑1 (MQ=255)
                                                                  tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACTCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                              <  1:2247152/150‑1 (MQ=255)
                                                                  tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                              <  1:4281871/150‑1 (MQ=255)
                                                                  tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                              <  1:683688/150‑1 (MQ=255)
                                                                   ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                             <  1:2207068/150‑1 (MQ=255)
                                                                          cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAt                                                                      >  2:2603813/1‑150 (MQ=255)
                                                                           tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAtt                                                                     <  1:2955340/150‑1 (MQ=255)
                                                                            gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTg                                                                    <  2:773688/150‑1 (MQ=255)
                                                                              cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGcc                                                                  >  2:1823541/1‑150 (MQ=255)
                                                                               gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAATGccc                                                                 >  1:1669274/1‑150 (MQ=255)
                                                                               gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGccc                                                                 <  1:473945/150‑1 (MQ=255)
                                                                                tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCg                                                                >  2:150406/1‑150 (MQ=255)
                                                                                tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGGGCTTTTTGCCCCGCATTCCGACGAAAATTGCCCg                                                                >  1:4072372/1‑150 (MQ=255)
                                                                                 ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                               >  2:3110225/1‑150 (MQ=255)
                                                                                 ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                               >  2:5057327/1‑150 (MQ=255)
                                                                                   aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGa                                                             <  2:3096548/150‑1 (MQ=255)
                                                                                   aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGa                                                             <  1:1893657/150‑1 (MQ=255)
                                                                                     cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAACATTGCCCGGGaag                                                           >  2:4466572/1‑149 (MQ=255)
                                                                                     cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGGCTGGAGTTTGCGCTTTTTGCCCCGCCGTCCGACGAAAATTGCCCGGGCAt                                                           >  2:1805762/1‑150 (MQ=255)
                                                                                      aGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAtt                                                          <  1:3997091/150‑1 (MQ=255)
                                                                                            cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                    <  1:1505383/150‑1 (MQ=255)
                                                                                            cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                    >  2:4257575/1‑150 (MQ=255)
                                                                                            cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTTTGGCTTTTTGGGTTTTTGGCCAGCATTAAGACGAAAAATGCCCGGGGATTGTGtaa                                                    >  1:4002302/1‑150 (MQ=255)
                                                                                               cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                 >  2:217421/1‑150 (MQ=255)
                                                                                                  gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAc                                              <  1:2841131/150‑1 (MQ=255)
                                                                                                   tAAAAACGATTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcg                                             <  1:4807692/150‑1 (MQ=255)
                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAGTGCCCGGGACTGGGGAAAAAATAcgc                                            >  1:5057274/1‑150 (MQ=255)
                                                                                                     aaaaCGCAATTCCAACTGTAGGCGCTTTTCCAGTAAGTAGATAAAAATTTAATGAAAAACCTCCTGACGCACGATTAGTTTATTCTCGATTTTTAGAATTTTGACCACCAATCATACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                           >  2:2480596/1‑150 (MQ=255)
                                                                                                      aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                          >  2:2123574/1‑150 (MQ=255)
                                                                                                       aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                                         <  2:2000942/150‑1 (MQ=255)
                                                                                                           cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGCCAgcg                                     >  1:4640266/1‑150 (MQ=255)
                                                                                                            aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTGGGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAAGTGTGTAAAAATAAGCGACAgcgc                                    >  1:2469285/1‑150 (MQ=255)
                                                                                                            aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                    >  2:2278177/1‑150 (MQ=255)
                                                                                                                gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                <  2:173363/150‑1 (MQ=255)
                                                                                                                 cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTTCGCTTTTTTCCCACCAATCAGACGAAAAATTCCCCGGAATTGTGAAAAAAAAAGCGACAccgcgccaa                               >  1:3559976/1‑147 (MQ=255)
                                                                                                                     tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCTTTTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                           <  1:4973777/150‑1 (MQ=255)
                                                                                                                     tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAATTTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                           <  1:2394643/150‑1 (MQ=255)
                                                                                                                       tCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCgg                         >  2:944717/1‑149 (MQ=255)
                                                                                                                       tCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGGGTGGATTTTGCGCTTTTTGCCCACCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAAAACCGt                         >  2:2723801/1‑150 (MQ=255)
                                                                                                                        cGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                        <  2:4683139/150‑1 (MQ=255)
                                                                                                                         ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                       <  2:1428722/150‑1 (MQ=255)
                                                                                                                           cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                     <  1:3731213/150‑1 (MQ=255)
                                                                                                                           cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAATCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCCTtctc                     >  1:799018/1‑150 (MQ=255)
                                                                                                                           cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                     >  2:346450/1‑150 (MQ=255)
                                                                                                                              ttttCCAGTATTTTGCTAAAAATTTTATGAAAAACGGGCTGCGGGCAGTTTTGTTTTGTCTGGATTTTGCGCTTATTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                  <  2:3402439/150‑1 (MQ=255)
                                                                                                                                 tCCAGTAGGTGGCTAAAAATTTGATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTGGCCCAGCATTCAGACGAAAATTGCCCTGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa               <  2:4102694/150‑1 (MQ=255)
                                                                                                                                  ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTTTCGCTTTTTTCCCAGCATTCAGACGAAAATTTCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAAAAAACGTTCTAGACTCAt              >  1:2771787/1‑150 (MQ=255)
                                                                                                                                    aGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa            <  1:3332866/150‑1 (MQ=255)
                                                                                                                                    aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGATGATTTTTGGCTTTTTTCCCAGCAATCAGACGAAAAATGCCCCGGAATTTTGAAAAAAAAATCGACAGCGCGCAAAAAACCTTCTAGACTCaaaa            >  1:2338598/1‑150 (MQ=255)
                                                                                                                                    aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa            >  2:3937132/1‑150 (MQ=255)
                                                                                                                                    aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa            >  1:417558/1‑150 (MQ=255)
                                                                                                                                    aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa            >  2:2781111/1‑150 (MQ=255)
                                                                                                                                     gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa           <  2:513378/150‑1 (MQ=255)
                                                                                                                                      tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa          >  1:4712160/1‑150 (MQ=255)
                                                                                                                                       aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg         <  1:920137/150‑1 (MQ=255)
                                                                                                                                          ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa      >  2:1922683/1‑150 (MQ=255)
                                                                                                                                           tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGGGCTTTTTTCCCAGCAATAAGACGAAAATTGCCCGGGAATTGTTAAAAAATAAGCGACCGCGCGCAAAAAAAGTTCTAGACTCATAAAAGTGAt     >  1:114491/1‑150 (MQ=255)
                                                                                                                                              tAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc  <  2:1073749/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450144‑450435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑