Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 477,267 G→T 100% Q865K (CAG→AAG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273477,2670GT99.4% 906.4 / ‑4.3 274Q865K (CAG→AAG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (137/135);  total (138/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

CAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCA  >  CP009273/477118‑477413
                                                                                                                                                     |                                                                                                                                                  
  gcAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTAt                                                                                                                                                  <  1:2194603/150‑1 (MQ=255)
   cAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTtata                                                                                                                                                 >  1:3171078/1‑150 (MQ=255)
    aaCGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                                >  1:2031926/1‑150 (MQ=255)
    aaCGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCAGGAGAGACGTTCCTTATAg                                                                                                                                                <  2:4341786/150‑1 (MQ=255)
         aCCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACAt                                                                                                                                           >  1:4941602/1‑150 (MQ=255)
         aCCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACAt                                                                                                                                           >  2:325485/1‑150 (MQ=255)
             aTAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATcccc                                                                                                                                       <  2:2162684/150‑1 (MQ=255)
              tAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCg                                                                                                                                      >  1:269406/1‑150 (MQ=255)
               aaCCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGt                                                                                                                                     <  1:2754199/150‑1 (MQ=255)
                aCCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                    >  2:3623006/1‑150 (MQ=255)
                aCCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                    <  2:2716931/150‑1 (MQ=255)
                   cccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAg                                                                                                                                 <  1:39518/150‑1 (MQ=255)
                    ccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGt                                                                                                                                >  1:2164757/1‑150 (MQ=255)
                       gCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCAt                                                                                                                             >  2:2323655/1‑150 (MQ=255)
                        cGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATa                                                                                                                            <  1:2991729/150‑1 (MQ=255)
                        cGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATa                                                                                                                            <  1:2991573/150‑1 (MQ=255)
                           aaCGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                         <  1:2610204/150‑1 (MQ=255)
                            aCGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCa                                                                                                                        <  1:4605239/150‑1 (MQ=255)
                               aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAaca                                                                                                                     >  1:3051368/1‑150 (MQ=255)
                                ccAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAacac                                                                                                                    <  1:3449268/150‑1 (MQ=255)
                                   gCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCgg                                                                                                                 >  1:4128047/1‑150 (MQ=255)
                                    cATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGt                                                                                                                <  1:2941465/150‑1 (MQ=255)
                                     aTAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTa                                                                                                               <  1:4739087/150‑1 (MQ=255)
                                      tAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                                                                              >  1:4382523/1‑150 (MQ=255)
                                      tAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                                                                              <  2:2449808/150‑1 (MQ=255)
                                      tAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                                                                              <  1:4661547/150‑1 (MQ=255)
                                       aaCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                                                                             >  2:3803943/1‑150 (MQ=255)
                                        aCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                                                                            <  1:580875/150‑1 (MQ=255)
                                         cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                                                                           >  2:121403/1‑150 (MQ=255)
                                         cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                                                                           <  2:1408928/150‑1 (MQ=255)
                                           gagaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                         >  1:3645677/1‑150 (MQ=255)
                                           gagaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                         <  2:2568768/150‑1 (MQ=255)
                                           gagaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                         <  2:440342/150‑1 (MQ=255)
                                           gagaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                         >  1:431281/1‑150 (MQ=255)
                                            tgaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                        >  2:2328688/2‑150 (MQ=255)
                                            agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                        <  2:2752821/150‑1 (MQ=255)
                                            agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                        <  1:1144329/150‑1 (MQ=255)
                                                 ggAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                                                                                   <  2:2655383/150‑1 (MQ=255)
                                                  gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACGGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCg                                                                                                  <  1:5149958/150‑1 (MQ=255)
                                                  gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAGGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCg                                                                                                  >  1:1814367/1‑150 (MQ=255)
                                                   aaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGc                                                                                                 >  2:4747329/1‑150 (MQ=255)
                                                     tCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCa                                                                                               <  1:2448619/150‑1 (MQ=255)
                                                         ccAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttg                                                                                           >  2:1377407/1‑150 (MQ=255)
                                                          cAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                                                                          <  2:1530070/150‑1 (MQ=255)
                                                           aGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                         <  1:1478504/150‑1 (MQ=255)
                                                           aGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                         <  1:699296/150‑1 (MQ=255)
                                                            gCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTc                                                                                        <  1:4479322/150‑1 (MQ=255)
                                                             ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcg                                                                                       <  2:1745065/150‑2 (MQ=255)
                                                              tctcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                                                                      <  1:5160697/150‑1 (MQ=255)
                                                               ctcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                                                                     <  1:1194567/150‑1 (MQ=255)
                                                                 cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCa                                                                                   <  2:3741629/150‑1 (MQ=255)
                                                                  gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAg                                                                                  >  2:3045329/1‑150 (MQ=255)
                                                                     cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                                                                               <  1:1843629/150‑1 (MQ=255)
                                                                        cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                                                                            >  1:840788/1‑150 (MQ=255)
                                                                           cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                                                                         >  1:2066025/1‑150 (MQ=255)
                                                                            gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                                                                        >  1:2292042/1‑150 (MQ=255)
                                                                            gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                                                                        <  2:3473274/150‑1 (MQ=255)
                                                                            gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                                                                        <  2:3824874/150‑1 (MQ=255)
                                                                                 acacAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTACTTATATGACATACCCGTCCAGTCATAGCCAACACCGGTAGGCAGTATGCTCGCCAGTTGTTCCATCAGCTCCATTGATTCAcc                                                                   >  2:4002577/1‑150 (MQ=255)
                                                                                   acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg                                                                 >  2:4876818/1‑150 (MQ=255)
                                                                                     aGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa                                                               >  1:1285566/1‑150 (MQ=255)
                                                                                     aGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa                                                               >  2:837433/1‑150 (MQ=255)
                                                                                       gAACACGACAATCAACGAAATCGCGTTCTGTGAAGGTGCCTGTTTGCCGTAGAGTCGTTCCTTATAGGACATCCCCGTCCAGTCATTGCAAACACCGGTAGGCTGTTTGATCGCCAGTTTTTCCATCAGCTCCATTGCTTCACCGGTACt                                                             <  1:327086/150‑1 (MQ=255)
                                                                                       gAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt                                                             <  2:2136707/150‑1 (MQ=255)
                                                                                          cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtttt                                                          <  1:4402274/150‑1 (MQ=255)
                                                                                                 aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGt                                                   >  1:3390091/1‑150 (MQ=255)
                                                                                                 aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGt                                                   >  1:806333/1‑150 (MQ=255)
                                                                                                    aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgcc                                                <  2:3166256/150‑1 (MQ=255)
                                                                                                       gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgcc                                             >  1:4859492/1‑150 (MQ=255)
                                                                                                        aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                            >  2:1896643/1‑150 (MQ=255)
                                                                                                        aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                            <  2:3588221/150‑1 (MQ=255)
                                                                                                         aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTg                                           >  1:3469957/1‑150 (MQ=255)
                                                                                                          aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGCCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTATTACCCGGTGCCGCCTgg                                          >  1:3904883/1‑150 (MQ=255)
                                                                                                                 aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGa                                   >  1:3822549/1‑150 (MQ=255)
                                                                                                                  cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAt                                  >  1:1745364/1‑150 (MQ=255)
                                                                                                                  cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGAAGTTACATAAGCTCCATAGCTTCACCTGTACTTTTACCCGGTGACGCCTGGCCTAAGAt                                  >  2:2540994/1‑150 (MQ=255)
                                                                                                                   aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAtt                                 <  2:3420232/150‑1 (MQ=255)
                                                                                                                   aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAtt                                 >  2:2038955/1‑150 (MQ=255)
                                                                                                                    gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAttt                                <  1:4100086/150‑1 (MQ=255)
                                                                                                                     tGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTc                               >  2:2210378/1‑150 (MQ=255)
                                                                                                                       aaGGTGCCTTGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCGCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCa                             >  2:172662/1‑150 (MQ=255)
                                                                                                                       aaGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCa                             >  2:1179428/1‑150 (MQ=255)
                                                                                                                         ggTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatg                           >  1:2797324/1‑150 (MQ=255)
                                                                                                                             ccTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatg                       <  2:5004411/150‑1 (MQ=255)
                                                                                                                              cTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatgg                      <  2:3839849/150‑1 (MQ=255)
                                                                                                                               tGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGc                     >  1:4951357/1‑150 (MQ=255)
                                                                                                                                ggTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCa                    >  1:1038685/1‑150 (MQ=255)
                                                                                                                                 gTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAg                   <  2:983398/150‑1 (MQ=255)
                                                                                                                                 gTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAg                   >  1:1346283/1‑150 (MQ=255)
                                                                                                                                 gTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAg                   <  2:2971821/150‑1 (MQ=255)
                                                                                                                                  ttGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAgg                  <  1:1425253/150‑1 (MQ=255)
                                                                                                                                     ccGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCg               <  1:1686254/150‑1 (MQ=255)
                                                                                                                                         agagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt           <  1:3759764/150‑1 (MQ=255)
                                                                                                                                         agagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt           >  1:426359/1‑150 (MQ=255)
                                                                                                                                         agagaCGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt           <  2:4062498/150‑1 (MQ=255)
                                                                                                                                          gagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTa          <  1:3615970/150‑1 (MQ=255)
                                                                                                                                           agaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa         >  2:2240645/1‑150 (MQ=255)
                                                                                                                                            gaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAAc        >  1:1658348/1‑150 (MQ=255)
                                                                                                                                               gTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGtt     <  2:662476/150‑1 (MQ=255)
                                                                                                                                                  ccTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCa  <  1:796530/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCA  >  CP009273/477118‑477413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑