Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 481,667 G→A 100% A151T (GCG→ACG)  acrR → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273481,6670GA99.2% 817.6 / ‑2.8 246A151T (GCG→ACG) acrRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (132/112);  total (132/114)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTG  >  CP009273/481520‑481813
                                                                                                                                                   |                                                                                                                                                  
 cAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGa                                                                                                                                                 >  1:518795/1‑150 (MQ=255)
  agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaa                                                                                                                                                <  1:2807005/150‑1 (MQ=255)
   gaagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaa                                                                                                                                               <  1:2957357/150‑1 (MQ=255)
   gaagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaa                                                                                                                                               <  1:4199473/150‑1 (MQ=255)
    aagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAAATTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaaa                                                                                                                                              >  1:4756707/1‑150 (MQ=255)
     agaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAt                                                                                                                                             <  2:4361218/150‑1 (MQ=255)
      gaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATg                                                                                                                                            <  2:3001391/150‑1 (MQ=255)
        aCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGtt                                                                                                                                          <  2:1873889/150‑1 (MQ=255)
        aCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGtt                                                                                                                                          >  1:2266918/1‑150 (MQ=255)
         cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                         <  1:2126755/150‑1 (MQ=255)
         cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                         >  2:2264691/1‑150 (MQ=255)
          ggCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCATAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGCAACGAAAATGGTGc                                                                                                                                        >  2:2867528/1‑150 (MQ=255)
           gCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGcc                                                                                                                                       <  1:2323058/150‑1 (MQ=255)
           gCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGcc                                                                                                                                       <  2:4979667/150‑1 (MQ=255)
           gCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGcc                                                                                                                                       >  2:2641568/1‑150 (MQ=255)
            cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                      <  1:1182656/150‑1 (MQ=255)
            cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                      <  2:4092322/150‑1 (MQ=255)
             gTCGATAAATTATGGAGATTATATAACACAAAATCTAATTTGTCTTAGAAATGGCTGTTTTGCAAAATGAACAAATTAATCTATGTAAGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTCTATTGAAACGAAAATGTTGCCTg                                                                                                                                     >  2:259921/1‑150 (MQ=255)
               cGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCg                                                                                                                                   >  2:5005454/1‑150 (MQ=255)
               cGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCg                                                                                                                                   >  1:337377/1‑150 (MQ=255)
                gATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCgg                                                                                                                                  >  2:1376924/1‑150 (MQ=255)
                   tattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAtt                                                                                                                               <  2:1733823/150‑1 (MQ=255)
                        aTGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATg                                                                                                                          <  2:1154455/150‑1 (MQ=255)
                           gagaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcg                                                                                                                       <  1:3653798/150‑1 (MQ=255)
                            agaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                      >  2:527911/1‑150 (MQ=255)
                               ttATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTc                                                                                                                   >  1:2790767/1‑150 (MQ=255)
                                    ttCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGATGCGTCGCgca                                                                                                              <  2:4543660/150‑1 (MQ=255)
                                     tCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgcag                                                                                                             >  1:688864/1‑150 (MQ=255)
                                         caAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAat                                                                                                         >  2:2326602/1‑150 (MQ=255)
                                         caAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAat                                                                                                         >  1:4230740/1‑150 (MQ=255)
                                          aaaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAatt                                                                                                        >  1:4965333/1‑150 (MQ=255)
                                           aaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAatta                                                                                                       <  2:3771428/150‑1 (MQ=255)
                                           aaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCACCAatta                                                                                                       <  1:3140462/150‑1 (MQ=255)
                                               cGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATtattat                                                                                                   <  2:1218872/150‑1 (MQ=255)
                                               cGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATtattat                                                                                                   <  1:2629955/150‑1 (MQ=255)
                                                 aaTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgc                                                                                                 >  2:2465237/1‑150 (MQ=255)
                                                 aaTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgc                                                                                                 >  2:278419/1‑150 (MQ=255)
                                                     tGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                             <  1:4278037/150‑1 (MQ=255)
                                                     tGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                             <  2:4855631/150‑1 (MQ=255)
                                                       tCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAACCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                           <  2:720997/150‑1 (MQ=255)
                                                        cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCta                                                                                          >  2:811100/1‑150 (MQ=255)
                                                          gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                        <  1:2052465/150‑1 (MQ=255)
                                                              aaTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAGGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTc                                                                                    >  1:438961/1‑150 (MQ=255)
                                                               aTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                   >  1:842506/1‑150 (MQ=255)
                                                               aTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                   <  2:1921954/150‑1 (MQ=255)
                                                                tGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCg                                                                                  <  2:4428991/150‑1 (MQ=255)
                                                                 ggCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCgg                                                                                 <  1:1775827/150‑1 (MQ=255)
                                                                 ggCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCgg                                                                                 >  1:4099704/1‑150 (MQ=255)
                                                                   cTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGcc                                                                               >  2:1677057/1‑150 (MQ=255)
                                                                    tgttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCt                                                                              >  1:133820/1‑150 (MQ=255)
                                                                     gttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTg                                                                             >  2:2861500/1‑150 (MQ=255)
                                                                     gttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAAAGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTg                                                                             <  1:1062842/150‑1 (MQ=255)
                                                                       tgtgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGAt                                                                           <  2:5150723/150‑1 (MQ=255)
                                                                         tgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATgg                                                                         <  1:1835609/150‑1 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAATTTATGACCGTATAGAACAAACGTTAAAACTTTGTATTGAAACGAATATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGAGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                            <  1:994116/150‑1 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGTAAACTGGCTCtt                                                            <  1:1171480/150‑1 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                            <  2:126991/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGTATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  1:2697455/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  1:4506115/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  2:2372769/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  1:710014/150‑1 (MQ=255)
                                                                                        gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                          >  2:2497979/1‑150 (MQ=255)
                                                                                        gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                          <  1:1584307/150‑1 (MQ=255)
                                                                                         tAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGc                                                         <  2:4756707/150‑1 (MQ=255)
                                                                                          aaTCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGcc                                                        >  2:2023477/1‑150 (MQ=255)
                                                                                           aTCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAACACTGGCTCTTTGccc                                                       >  2:2435930/1‑150 (MQ=255)
                                                                                           aTCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGccc                                                       >  1:4686899/1‑150 (MQ=255)
                                                                                            tctcTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGcccc                                                      <  2:2282600/150‑1 (MQ=255)
                                                                                             ctctGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGcccca                                                     <  2:3406295/150‑2 (MQ=255)
                                                                                                 gtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAAt                                                 <  1:1976385/150‑1 (MQ=255)
                                                                                                       aaaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTg                                           >  1:2369793/1‑150 (MQ=255)
                                                                                                        aaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGa                                          <  1:243201/150‑1 (MQ=255)
                                                                                                           ttATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCt                                       <  1:5032423/150‑1 (MQ=255)
                                                                                                           ttATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCt                                       <  1:4855666/150‑1 (MQ=255)
                                                                                                           ttATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCt                                       <  1:4855580/150‑1 (MQ=255)
                                                                                                            tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                      >  2:2751429/1‑150 (MQ=255)
                                                                                                            tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                      >  2:4629416/1‑150 (MQ=255)
                                                                                                             aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTa                                     >  2:1479064/1‑150 (MQ=255)
                                                                                                                aCCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaa                                  <  2:4297000/150‑1 (MQ=255)
                                                                                                                   gTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaag                               >  1:3848786/1‑150 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                              >  1:2506323/1‑150 (MQ=255)
                                                                                                                       aGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                           <  2:906580/150‑1 (MQ=255)
                                                                                                                       aGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                           >  1:5150639/1‑150 (MQ=255)
                                                                                                                       aGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                           >  2:4205015/1‑150 (MQ=255)
                                                                                                                        gAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGcc                          >  2:1581117/1‑150 (MQ=255)
                                                                                                                         aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                         >  1:4897212/1‑150 (MQ=255)
                                                                                                                         aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                         >  1:1988251/1‑150 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                   <  2:4843091/150‑1 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                   <  2:2103820/150‑1 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAAGTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                   >  2:1454535/1‑150 (MQ=255)
                                                                                                                                      aCATTGGATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGc            <  1:3129956/150‑1 (MQ=255)
                                                                                                                                       cATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGcc           >  2:4732992/1‑150 (MQ=255)
                                                                                                                                       cATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGcc           >  2:3381018/1‑150 (MQ=255)
                                                                                                                                        aTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCa          <  1:1376924/150‑1 (MQ=255)
                                                                                                                                           gTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt       >  2:3803146/1‑150 (MQ=255)
                                                                                                                                           gTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt       >  2:3949484/1‑150 (MQ=255)
                                                                                                                                           gTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt       >  1:1664070/1‑150 (MQ=255)
                                                                                                                                            tATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCtt      >  1:3021048/1‑150 (MQ=255)
                                                                                                                                             aTTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTa     <  2:3815402/150‑1 (MQ=255)
                                                                                                                                               tGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACt   >  2:680591/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTG  >  CP009273/481520‑481813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑