Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 503,944 G→T 100% E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT98.3% 792.4 / ‑1.7 270E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (142/116);  total (150/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

AGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  CP009273/503797‑504089
                                                                                                                                                   |                                                                                                                                                 
aGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACAAGCGCACCCAGgaataa                                                                                                                                                 >  1:3556892/1‑150 (MQ=255)
aGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGgaataa                                                                                                                                                 <  2:1200435/150‑1 (MQ=255)
     aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTggg                                                                                                                                            >  2:869656/1‑150 (MQ=255)
       tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGAc                                                                                                                                          >  2:4637010/1‑150 (MQ=255)
        gATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACg                                                                                                                                         <  1:1110287/150‑1 (MQ=255)
            aaTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATTCGCACCCAGGAAGAATTGGGACGGAtt                                                                                                                                     >  1:3614207/1‑150 (MQ=255)
               aaCAATCGTAAACTCACTACTGAGATGGCATTTCTTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCAAAACCTCCACGCCGCGGTTGTTCGTTTGGAATTTGAAAACAACATGCGCACCCAGGCATAATTGGGACGGAATGaa                                                                                                                                  >  2:1878195/1‑150 (MQ=255)
                   aTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAg                                                                                                                              <  1:3096630/150‑1 (MQ=255)
                    tCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGt                                                                                                                             >  2:2565406/1‑150 (MQ=255)
                     cGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTg                                                                                                                            >  2:4337398/1‑150 (MQ=255)
                       tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGTAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACACTGGc                                                                                                                          >  1:3291607/1‑150 (MQ=255)
                         aaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTg                                                                                                                        <  1:1845203/150‑1 (MQ=255)
                           cTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGaa                                                                                                                      >  2:2177521/1‑150 (MQ=255)
                            tCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAat                                                                                                                     <  1:3107212/150‑1 (MQ=255)
                             cACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGCTTGAAACAGTGGCTGAata                                                                                                                    >  1:393370/1‑150 (MQ=255)
                              actactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCCGGCCGCGGTTGTTAGTTGGGAAGTTTTACACAACATGCGCCCCCAGGTATATTTGGGTCGGGTTGAAACAGTGGCGGagttt                                                                                                                   >  1:4123854/1‑146 (MQ=255)
                               ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATAtt                                                                                                                  >  1:280627/1‑150 (MQ=255)
                                 actGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGCTCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTg                                                                                                                >  2:167687/1‑150 (MQ=255)
                                    gagaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCa                                                                                                             <  2:907365/150‑1 (MQ=255)
                                    gagaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCa                                                                                                             >  1:3279580/1‑150 (MQ=255)
                                       aTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTTAACCACCAGGCGGCGGGTGATCTTTGGGAAGTTGTAAAAAACATGCGCACCCCGGAATAAATTGGACGGATTGAAACAGTGGCTGAATATTTTGCACGc                                                                                                          >  1:4049881/1‑150 (MQ=255)
                                       aTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGTTCTTTGGGAAGTTGTAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGTATATTTGGCACGc                                                                                                          >  2:2030629/1‑150 (MQ=255)
                                       aTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGTAAACAACAAGCGCACCCAGGAATAATTGGGGCGGATTGAAACAGTGGCTGAATATTTTGCACGc                                                                                                          >  1:5134075/1‑150 (MQ=255)
                                        tGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGATCGTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGcc                                                                                                         >  1:1617116/1‑150 (MQ=255)
                                           cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                      <  2:3161574/150‑1 (MQ=255)
                                           cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                      <  2:1612384/150‑1 (MQ=255)
                                               tCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAaga                                                                                                  <  2:2501082/150‑1 (MQ=255)
                                                cGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATTCGCACCCAGGAATAAATGGGCCGGGGTGAAACAGGGGCTGGAAATTTGGCACGCCGTGAagag                                                                                                 >  1:4294423/1‑150 (MQ=255)
                                                 gTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGc                                                                                                <  2:2928339/150‑1 (MQ=255)
                                                  tCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGATCTTCGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCg                                                                                               >  2:1706713/1‑150 (MQ=255)
                                                     ggCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGc                                                                                            >  2:760837/1‑150 (MQ=255)
                                                     ggCGAAAGTTTTTGAGACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGc                                                                                            <  2:3606428/150‑1 (MQ=255)
                                                                 tGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCCAAAAAGGTcgc                                                                                >  1:2600000/1‑150 (MQ=255)
                                                                  gATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcgcg                                                                               <  2:427393/150‑1 (MQ=255)
                                                                  gATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcgcg                                                                               <  1:808566/150‑1 (MQ=255)
                                                                     aCCACAGTCGATTTTTGGCTAAACCTCGAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCAGCCAGGATTAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTaa                                                                            <  1:1878195/150‑1 (MQ=255)
                                                                      ccACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAg                                                                           >  2:4792173/1‑150 (MQ=255)
                                                                       cacaGTAGATTTTTGTCTAAACCTCCAGGAGGCGGTTGATCTGTGGGAAGTTGAAAACTACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAgg                                                                          <  2:2694171/150‑1 (MQ=255)
                                                                         caGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGcc                                                                        >  1:1440845/1‑150 (MQ=255)
                                                                         caGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGcc                                                                        >  1:755945/1‑150 (MQ=255)
                                                                          aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                       >  1:2326454/1‑150 (MQ=255)
                                                                           gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                      <  2:4439248/150‑1 (MQ=255)
                                                                              gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                   <  1:2989714/150‑1 (MQ=255)
                                                                               aTTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                  >  1:3333414/1‑150 (MQ=255)
                                                                               aTTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                  >  1:2192791/1‑150 (MQ=255)
                                                                                tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGTGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTTCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                 <  1:2116889/150‑1 (MQ=255)
                                                                                tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                 >  1:4690674/1‑150 (MQ=255)
                                                                                  tttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                               >  1:973426/1‑150 (MQ=255)
                                                                                  tttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                               <  1:1222781/150‑1 (MQ=255)
                                                                                    tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                             <  2:3937560/150‑1 (MQ=255)
                                                                                    tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                             <  2:2580840/150‑1 (MQ=255)
                                                                                     ggCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCtt                                                            <  2:4104818/150‑1 (MQ=255)
                                                                                      gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                           >  1:1028409/1‑150 (MQ=255)
                                                                                      gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                           <  1:4284886/150‑1 (MQ=255)
                                                                                       cTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                          >  1:4858718/1‑150 (MQ=255)
                                                                                       cTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                          <  1:2606378/150‑1 (MQ=255)
                                                                                       cTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                          >  2:573908/1‑150 (MQ=255)
                                                                                         aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGACGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCTCTTCAtc                                                        >  1:4772721/1‑150 (MQ=255)
                                                                                          aaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtct                                                       <  2:1569826/150‑1 (MQ=255)
                                                                                            ccTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCg                                                     >  2:3769798/1‑150 (MQ=255)
                                                                                              tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                   <  2:4771162/150‑1 (MQ=255)
                                                                                              tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGCAGAGCGTGCAAATAAGGTCGCGTAAGGCGAAAACATCGCGTCATCTCGTa                                                   >  2:2683975/1‑150 (MQ=255)
                                                                                                cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAgg                                                 >  1:870771/1‑150 (MQ=255)
                                                                                                 aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                >  2:342905/1‑150 (MQ=255)
                                                                                                 aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                >  2:2333842/1‑150 (MQ=255)
                                                                                                  ggcggcGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGcc                                               >  1:2490103/1‑150 (MQ=255)
                                                                                                     ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                            >  2:4886325/1‑150 (MQ=255)
                                                                                                     ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                            >  2:5028280/1‑150 (MQ=255)
                                                                                                       cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                          >  1:4573055/1‑150 (MQ=255)
                                                                                                       cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                          >  1:2376915/1‑150 (MQ=255)
                                                                                                       cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTGCATCTCGTAGGCCGGATa                                          >  2:2234744/1‑150 (MQ=255)
                                                                                                         gTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAg                                        >  2:3824266/1‑150 (MQ=255)
                                                                                                             aTCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCACAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGt                                    >  2:3965014/1‑150 (MQ=255)
                                                                                                             aTCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGt                                    <  1:2487813/150‑1 (MQ=255)
                                                                                                              tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGtt                                   <  2:4374814/150‑1 (MQ=255)
                                                                                                              tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGtt                                   <  2:4233658/150‑1 (MQ=255)
                                                                                                               cTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTc                                  >  1:2863732/1‑150 (MQ=255)
                                                                                                                 ttGGGAAGTTGAAAACAACATGCGCAACCAGGAATAATTGGGACTGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGAGTGCAAAAAATGTCGCGTAAGGCCAAAACATCGATTCATCTCGTAGGCCGGATAAGGCGTTCAc                                <  1:812596/150‑1 (MQ=255)
                                                                                                                   gggAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgc                              >  1:2007995/1‑150 (MQ=255)
                                                                                                                    ggAAGTTGAACACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgcc                             <  2:4375551/150‑1 (MQ=255)
                                                                                                                      aaGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccgc                           >  2:2886425/1‑150 (MQ=255)
                                                                                                                         ttGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatc                        >  1:617582/1‑150 (MQ=255)
                                                                                                                           gAAAACAACATGCGCACCCAGGAATAATGGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                      <  2:3147660/150‑1 (MQ=255)
                                                                                                                            aaaaCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccgg                     <  1:2359827/150‑1 (MQ=255)
                                                                                                                            aaaaCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccgg                     >  2:1477096/1‑150 (MQ=255)
                                                                                                                             aaaCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggc                    >  1:4718244/1‑150 (MQ=255)
                                                                                                                             aaaCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggc                    >  2:3674039/1‑150 (MQ=255)
                                                                                                                                    aTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg             <  2:2014812/150‑1 (MQ=255)
                                                                                                                                    aTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg             <  1:3074236/150‑1 (MQ=255)
                                                                                                                                     tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGc            <  2:4573055/150‑1 (MQ=255)
                                                                                                                                     tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGc            >  2:1115464/1‑150 (MQ=255)
                                                                                                                                        gcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAcc         >  1:4492696/1‑150 (MQ=255)
                                                                                                                                          aCCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAt       >  2:4147339/1‑150 (MQ=255)
                                                                                                                                           cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt      <  1:4426201/150‑1 (MQ=255)
                                                                                                                                             cAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGc    >  2:3118384/1‑150 (MQ=255)
                                                                                                                                             cAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGc    >  1:4996248/1‑150 (MQ=255)
                                                                                                                                             cAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGc    <  1:3201517/150‑1 (MQ=255)
                                                                                                                                             cAGGAATAATGGGGACGGATTGAAACAGTGACTGCATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACCTCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGc    <  2:2945740/150‑1 (MQ=38)
                                                                                                                                              aGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGcc   <  1:183607/150‑1 (MQ=255)
                                                                                                                                               ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt  <  1:1251891/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  CP009273/503797‑504089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑