Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 601,847 A→T 100% I331I (ATT→ATA)  ybdK ← weak gamma‑glutamyl:cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273601,8470AT97.2% 1010.0 / 10.8 315I331I (ATT→ATA) ybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base T (161/144);  total (165/150)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 320 total aligned reads displayed.

TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTT  >  CP009273/601698‑601993
                                                                                                                                                     |                                                                                                                                                  
   tctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAAGGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATc                                                                                                                                                 <  2:361893/150‑1 (MQ=255)
    ctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcg                                                                                                                                                >  2:3835992/1‑150 (MQ=255)
    ctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcg                                                                                                                                                >  1:441490/1‑150 (MQ=255)
     tGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcgc                                                                                                                                               >  2:1307359/1‑150 (MQ=255)
     tGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcgc                                                                                                                                               <  2:4545985/150‑1 (MQ=255)
      gTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATcgcg                                                                                                                                              >  2:96368/1‑150 (MQ=255)
       tAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCgcgc                                                                                                                                             >  1:4278888/1‑150 (MQ=255)
         aGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTc                                                                                                                                           >  1:2722280/1‑150 (MQ=255)
             aaTTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATg                                                                                                                                       >  2:2391946/1‑150 (MQ=255)
              aTTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGc                                                                                                                                      >  2:1178361/1‑150 (MQ=255)
              aTTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGc                                                                                                                                      >  1:1562233/1‑150 (MQ=255)
               ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCa                                                                                                                                     >  1:4130351/1‑150 (MQ=255)
               ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCa                                                                                                                                     <  1:2931642/150‑1 (MQ=255)
                tCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCAc                                                                                                                                    >  1:4206577/1‑150 (MQ=255)
                 cccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCAcc                                                                                                                                   >  2:3589331/1‑150 (MQ=255)
                  ccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCa                                                                                                                                  >  1:3428256/1‑150 (MQ=255)
                       ttAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAtttt                                                                                                                             >  1:1372555/1‑150 (MQ=255)
                          gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                          <  2:4238532/150‑1 (MQ=255)
                          gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                          >  1:3000223/1‑150 (MQ=255)
                            cACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtgtg                                                                                                                        >  1:4477785/1‑150 (MQ=255)
                             aCCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGc                                                                                                                       >  1:3069952/1‑150 (MQ=255)
                               cGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCgg                                                                                                                     >  2:1752559/1‑150 (MQ=255)
                                 gCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGaa                                                                                                                   >  2:4483512/1‑150 (MQ=255)
                                  cccAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAg                                                                                                                  <  1:5076744/150‑1 (MQ=255)
                                    cAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                                >  1:2922288/1‑150 (MQ=255)
                                    cAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                                <  1:2621501/150‑1 (MQ=255)
                                      gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGc                                                                                                              <  2:1076672/150‑1 (MQ=255)
                                         ctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAt                                                                                                           >  1:2243360/1‑150 (MQ=255)
                                          tcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtt                                                                                                          >  1:4327109/1‑150 (MQ=255)
                                          tcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtt                                                                                                          <  2:3210565/150‑1 (MQ=255)
                                            acaATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtttt                                                                                                        >  1:2431210/1‑150 (MQ=255)
                                             ccctGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttttt                                                                                                       <  2:4794364/147‑1 (MQ=255)
                                             caATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTTCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttttt                                                                                                       >  1:3142887/1‑150 (MQ=255)
                                             caATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttttt                                                                                                       <  1:1302954/150‑1 (MQ=255)
                                               aTGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTc                                                                                                     <  2:1950591/150‑1 (MQ=255)
                                                tGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTcc                                                                                                    <  1:4849351/150‑1 (MQ=255)
                                                tGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTcc                                                                                                    <  2:336988/150‑1 (MQ=255)
                                                 gCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCa                                                                                                   >  1:2117182/1‑150 (MQ=255)
                                                  cTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAg                                                                                                  <  2:4132109/150‑1 (MQ=255)
                                                  cTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAg                                                                                                  <  1:4070785/150‑1 (MQ=255)
                                                  cTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAg                                                                                                  <  2:567650/150‑1 (MQ=255)
                                                      ttCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAAt                                                                                              <  1:1465122/150‑1 (MQ=255)
                                                      ttCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAAt                                                                                              >  1:368609/1‑150 (MQ=255)
                                                      ttCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAAt                                                                                              <  1:1222388/150‑1 (MQ=255)
                                                       tCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGACGGGGCGATTTTTTCCAGCAATc                                                                                             >  2:4875741/1‑150 (MQ=255)
                                                        cACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCg                                                                                            <  2:1626363/150‑1 (MQ=255)
                                                          ccAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCa                                                                                          >  2:4303982/1‑150 (MQ=255)
                                                            aGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAg                                                                                        >  1:4650302/1‑150 (MQ=255)
                                                                cAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTAt                                                                                    >  2:440531/1‑150 (MQ=255)
                                                                     aGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTcc                                                                               >  1:655945/1‑150 (MQ=255)
                                                                               cATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTc                                                                     <  2:3082303/150‑1 (MQ=255)
                                                                                aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAg                                                                          <  2:4334854/144‑1 (MQ=255)
                                                                                aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcg                                                                    >  1:2033728/1‑150 (MQ=255)
                                                                                aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcg                                                                    <  2:4165840/150‑1 (MQ=255)
                                                                                aTCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcg                                                                    <  1:1798845/150‑1 (MQ=255)
                                                                                         gaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTcc                                                           >  1:644557/1‑150 (MQ=255)
                                                                                         gaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTcc                                                           >  1:540279/1‑150 (MQ=255)
                                                                                         gaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTcc                                                           >  1:171325/1‑150 (MQ=255)
                                                                                                cgcATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGc                                                    <  2:1197555/150‑1 (MQ=255)
                                                                                                    ttAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGAt                                                <  1:4483512/150‑1 (MQ=255)
                                                                                                        cTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGt                                            <  1:96368/150‑1 (MQ=255)
                                                                                                         tGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtg                                           <  1:360013/150‑1 (MQ=255)
                                                                                                           cgcTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgat                                         >  1:4784318/1‑150 (MQ=255)
                                                                                                               tcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACg                                     <  2:5159774/150‑1 (MQ=255)
                                                                                                                  ttcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCt                                  <  2:2744040/150‑1 (MQ=255)
                                                                                                                    caGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTc                                >  2:2157920/1‑150 (MQ=255)
                                                                                                                    caGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTc                                <  1:5159184/150‑1 (MQ=255)
                                                                                                                    caGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTc                                <  1:3276607/150‑1 (MQ=255)
                                                                                                                      gACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCaa                              <  2:2372601/150‑1 (MQ=255)
                                                                                                                       aCCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAg                             <  1:2161085/150‑1 (MQ=255)
                                                                                                                        ccGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATCTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGc                            >  2:327209/1‑150 (MQ=255)
                                                                                                                         cGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGcc                           >  1:2490459/1‑150 (MQ=255)
                                                                                                                          gCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGccc                          >  2:2453448/1‑150 (MQ=255)
                                                                                                                           cTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCa                         >  2:2953109/1‑150 (MQ=255)
                                                                                                                            tGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCAt                        >  2:848356/1‑150 (MQ=255)
                                                                                                                             gacgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATa                       >  1:2344281/1‑150 (MQ=255)
                                                                                                                              acgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAg                      >  2:3041273/1‑150 (MQ=255)
                                                                                                                               cgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGc                     <  2:3959408/150‑1 (MQ=255)
                                                                                                                                 acCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGa                   <  2:4886254/150‑1 (MQ=255)
                                                                                                                                 acCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGa                   >  2:4974393/1‑150 (MQ=255)
                                                                                                                                 acCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGa                   <  2:2325885/150‑1 (MQ=255)
                                                                                                                                  ccTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGAc                  <  2:2522390/150‑1 (MQ=255)
                                                                                                                                    tGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg                >  1:4366199/1‑150 (MQ=255)
                                                                                                                                    tGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg                <  2:2265393/150‑1 (MQ=255)
                                                                                                                                    tGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg                >  2:2710853/1‑150 (MQ=255)
                                                                                                                                    tGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAg                <  2:45866/150‑1 (MQ=255)
                                                                                                                                     ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAgg               <  2:4477785/150‑1 (MQ=255)
                                                                                                                                     ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAgg               >  1:808417/1‑150 (MQ=255)
                                                                                                                                     ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAgg               <  2:2016182/150‑1 (MQ=255)
                                                                                                                                     ggCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAgg               <  2:1907000/150‑1 (MQ=255)
                                                                                                                                       cGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGcc             <  1:1110683/150‑1 (MQ=255)
                                                                                                                                       cGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGcc             <  1:4007180/150‑1 (MQ=255)
                                                                                                                                        gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCt            >  1:1020599/1‑150 (MQ=255)
                                                                                                                                        gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTACAGCAATCTCAAGGTATCTTCCGTTAGCGGTCGACGTTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCt            <  1:566519/150‑1 (MQ=255)
                                                                                                                                        gATGCAGGGCCTCGATCGCGCTCGAGGCACCTCTGTCAGGTGCGGAAGGGCCGCTTTTTTCCAGCAATCGCACGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCt            <  2:5082184/150‑1 (MQ=255)
                                                                                                                                          tGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTgg          >  2:4024104/1‑150 (MQ=255)
                                                                                                                                          tGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTgg          >  1:3811198/1‑150 (MQ=255)
                                                                                                                                            cAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaa        >  1:204155/1‑150 (MQ=255)
                                                                                                                                              gggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAAc      <  1:1592711/150‑1 (MQ=255)
                                                                                                                                                gCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACgg    <  2:675479/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTT  >  CP009273/601698‑601993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑