Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,302 G→T 100% R18S (AGG→AGT)  ybhM → UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,3020GT98.4% 655.2 / 0.6 207R18S (AGG→AGT) ybhMUPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (121/82);  total (124/83)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

TTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTA  >  CP009273/816155‑816447
                                                                                                                                                   |                                                                                                                                                 
tttCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTat                                                                                                                                                 <  2:1682467/150‑1 (MQ=255)
  tCTTTATCCATAAATGCAAAAGTATTTTGTATGCTTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTatat                                                                                                                                               <  2:4398672/150‑1 (MQ=255)
   cTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATAtt                                                                                                                                              <  2:3380494/150‑1 (MQ=255)
     gTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTaa                                                                                                                                            >  2:1744883/1‑150 (MQ=255)
     gTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTaa                                                                                                                                            <  1:270949/150‑1 (MQ=255)
      tATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTaaa                                                                                                                                           <  1:65682/150‑1 (MQ=255)
         ccATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACgg                                                                                                                                        >  1:3102451/1‑150 (MQ=255)
          cATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGt                                                                                                                                       >  1:625337/1‑150 (MQ=255)
           aTAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGta                                                                                                                                      >  1:1487678/1‑150 (MQ=255)
             aaaTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGtata                                                                                                                                    <  1:1283802/150‑1 (MQ=255)
             aaaTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGtata                                                                                                                                    >  1:562026/1‑150 (MQ=255)
             aaaTGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGtata                                                                                                                                    >  2:4127753/1‑150 (MQ=255)
                 gCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGc                                                                                                                                >  1:77419/1‑150 (MQ=255)
                 gCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGc                                                                                                                                >  2:2252266/1‑150 (MQ=255)
                   aaaaGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTc                                                                                                                              <  1:2189088/150‑1 (MQ=255)
                     aaGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCAt                                                                                                                            >  2:112940/1‑150 (MQ=255)
                      aGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCAtt                                                                                                                           <  2:1977180/150‑1 (MQ=255)
                         aTTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTAcc                                                                                                                        <  1:1730147/150‑1 (MQ=255)
                         aTTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTAcc                                                                                                                        >  2:2621680/1‑150 (MQ=255)
                             tGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCtt                                                                                                                    >  1:4456541/1‑150 (MQ=255)
                                aTGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAg                                                                                                                 >  1:1510179/1‑150 (MQ=255)
                                 tGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAgg                                                                                                                >  1:2428063/1‑150 (MQ=255)
                                   cGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGtt                                                                                                              >  1:3616087/1‑150 (MQ=255)
                                   cGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGtt                                                                                                              >  2:842016/1‑150 (MQ=255)
                                   cGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGtt                                                                                                              <  2:2276038/150‑1 (MQ=255)
                                   cGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGtt                                                                                                              <  1:1111244/150‑1 (MQ=255)
                                    gTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGttt                                                                                                             >  1:2982531/1‑150 (MQ=255)
                                       gTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggt                                                                                                          <  2:5042382/150‑1 (MQ=255)
                                        ttttAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGTATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTATATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTgtggtg                                                                                                         >  2:4183127/1‑150 (MQ=255)
                                        ttttAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggtg                                                                                                         <  1:1185320/150‑1 (MQ=255)
                                        ttttAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTtggtg                                                                                                         <  1:133341/150‑1 (MQ=255)
                                             aaTTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAAc                                                                                                    >  2:3165932/1‑150 (MQ=255)
                                                     gATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGAc                                                                                            <  2:4764270/150‑1 (MQ=255)
                                                      aTGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                           <  2:2253004/150‑1 (MQ=255)
                                                      aTGGTGAATATTAATATTTTGTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACt                                                                                           <  1:3963815/150‑1 (MQ=255)
                                                       tGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTa                                                                                          <  1:4073181/150‑1 (MQ=255)
                                                        ggTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAg                                                                                         <  2:2133823/150‑1 (MQ=255)
                                                           gAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCt                                                                                      >  1:4155964/1‑150 (MQ=255)
                                                           gAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTGAGGTTTGGTGGCAACCGCAGGACTAGCCt                                                                                      >  2:2383839/1‑150 (MQ=255)
                                                                ttaatattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGa                                                                                 <  2:625337/150‑1 (MQ=255)
                                                                   atattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCt                                                                              >  1:1264637/1‑150 (MQ=255)
                                                                    tattTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTa                                                                             <  1:3351800/150‑1 (MQ=255)
                                                                     attTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTaa                                                                            <  1:1571231/150‑1 (MQ=255)
                                                                      ttttttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTCAg                                                                           >  2:182772/1‑150 (MQ=255)
                                                                      ttttttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAg                                                                           <  1:4241703/150‑1 (MQ=255)
                                                                         tttCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGtat                                                                        >  1:4787652/1‑150 (MQ=255)
                                                                            cTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCCGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATc                                                                     >  2:3705820/1‑150 (MQ=255)
                                                                            cTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATc                                                                     >  2:4201056/1‑150 (MQ=255)
                                                                             tAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCg                                                                    >  1:1774356/1‑150 (MQ=255)
                                                                             tAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCg                                                                    >  2:1005480/1‑150 (MQ=255)
                                                                             tAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTAAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCg                                                                    >  2:3972813/1‑150 (MQ=255)
                                                                               aGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATCTCGaa                                                                  >  2:2856595/1‑150 (MQ=255)
                                                                                ggATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGaaa                                                                 >  2:4706438/1‑150 (MQ=255)
                                                                                  aTAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAAtt                                                               <  1:953300/150‑1 (MQ=255)
                                                                                      cTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACgg                                                           >  1:2518222/1‑150 (MQ=255)
                                                                                      cTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACgg                                                           <  2:4971381/150‑1 (MQ=255)
                                                                                        aaaaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCa                                                         <  2:1547905/150‑1 (MQ=255)
                                                                                          aaGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAc                                                       <  1:2097648/150‑1 (MQ=255)
                                                                                           aGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAcc                                                      <  1:4559113/150‑1 (MQ=255)
                                                                                            gAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCa                                                     >  2:3477862/1‑150 (MQ=255)
                                                                                            gAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCa                                                     >  2:4538425/1‑150 (MQ=255)
                                                                                               tGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAat                                                  >  1:1330319/1‑150 (MQ=255)
                                                                                                ggAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAata                                                 >  1:4383251/1‑150 (MQ=255)
                                                                                                 gAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAatat                                                <  2:2242176/150‑1 (MQ=255)
                                                                                                  aaTCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATg                                               >  1:3401593/1‑150 (MQ=255)
                                                                                                    tCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAt                                             >  2:4518960/1‑150 (MQ=255)
                                                                                                     cATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGAtt                                            <  1:431587/150‑1 (MQ=255)
                                                                                                      aTACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTc                                           >  2:3925113/1‑150 (MQ=255)
                                                                                                       tACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTca                                          >  2:1974374/1‑150 (MQ=255)
                                                                                                        aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcac                                         >  1:3178941/1‑150 (MQ=255)
                                                                                                        aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGGTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcac                                         >  1:3415309/1‑150 (MQ=255)
                                                                                                         cTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcacc                                        >  2:2049288/1‑150 (MQ=255)
                                                                                                           cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                      >  2:131633/1‑150 (MQ=255)
                                                                                                           cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                      >  2:159068/1‑150 (MQ=255)
                                                                                                           cGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTcaccac                                      >  2:3876157/1‑150 (MQ=255)
                                                                                                            gCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCaccacc                                     >  2:3433934/1‑150 (MQ=255)
                                                                                                             cAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCAccacca                                    >  2:1753819/1‑150 (MQ=255)
                                                                                                              aaaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCaa                                   >  1:4040936/1‑150 (MQ=255)
                                                                                                               aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                  <  2:2220938/150‑1 (MQ=255)
                                                                                                               aaaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAat                                  >  2:5126418/1‑150 (MQ=255)
                                                                                                                aaCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatg                                 <  2:2718100/150‑1 (MQ=255)
                                                                                                                 aCAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgt                                <  1:5100626/150‑1 (MQ=255)
                                                                                                                  cAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgta                               >  2:3208437/1‑150 (MQ=255)
                                                                                                                  cAGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgta                               >  2:4344929/1‑150 (MQ=255)
                                                                                                                   aGCAATAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAatgtat                              >  1:287059/1‑150 (MQ=255)
                                                                                                                      aaTAAATTAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTc                           >  1:438039/1‑150 (MQ=255)
                                                                                                                            ttAGATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATa                     <  2:408163/150‑1 (MQ=255)
                                                                                                                               gATTTTCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAggg                  <  2:364919/150‑1 (MQ=255)
                                                                                                                                  tttCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGtta               >  1:3226318/1‑150 (MQ=255)
                                                                                                                                  tttCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGtta               >  1:4881943/1‑150 (MQ=255)
                                                                                                                                  tttCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAAACAAATATGATTCACCACCAATGTATGTCGCCATAGGGtta               <  2:3321529/150‑1 (MQ=255)
                                                                                                                                    tCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatt             >  2:145783/1‑150 (MQ=255)
                                                                                                                                    tCAGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTAGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttatt             <  1:2252287/150‑1 (MQ=255)
                                                                                                                                      aGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACTACCAATGTATGTCGCCATAGGGttattat           >  1:804514/1‑150 (MQ=255)
                                                                                                                                      aGCACGAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGttattat           <  1:2621680/150‑1 (MQ=255)
                                                                                                                                           gAGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACtt      >  1:3989265/1‑150 (MQ=255)
                                                                                                                                            aGGCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACttt     >  1:3703227/1‑150 (MQ=255)
                                                                                                                                            aGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACttt     >  2:3809443/1‑150 (MQ=255)
                                                                                                                                             ggCCAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTtg    <  1:1005480/150‑1 (MQ=255)
                                                                                                                                               ccAGTATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCACCCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATCTGCTGCACCACCAATGTATGTCGCCATAGGGTGATTAGTACTgtgtt  >  2:4388978/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTTTTTCTAAGGATAACTAAAAGAATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTA  >  CP009273/816155‑816447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑