Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 829,009 G→T 100% D162Y (GAT→TAT)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273829,0090GT96.1% 943.9 / 22.2 309D162Y (GAT→TAT) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (9/3);  new base T (152/145);  total (161/148)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 309 total aligned reads displayed.

TTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTC  >  CP009273/828862‑829156
                                                                                                                                                   |                                                                                                                                                   
 ttGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAtc                                                                                                                                                  >  2:1999344/1‑150 (MQ=255)
   gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAAACAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAtctc                                                                                                                                                <  1:1737944/150‑1 (MQ=255)
      cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAt                                                                                                                                             >  1:4126606/1‑150 (MQ=255)
       gtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGAAGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATa                                                                                                                                            >  1:3067657/1‑150 (MQ=255)
         gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACt                                                                                                                                          >  1:4229039/1‑150 (MQ=255)
            cgcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtat                                                                                                                                       <  1:3616645/150‑1 (MQ=255)
            cgcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtat                                                                                                                                       >  1:4661375/1‑150 (MQ=255)
            cgcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtat                                                                                                                                       <  2:3792422/150‑1 (MQ=255)
             gcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtata                                                                                                                                      >  2:4496816/1‑150 (MQ=255)
             gcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtata                                                                                                                                      >  2:4066589/1‑150 (MQ=255)
               tACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaaa                                                                                                                                    >  1:1976190/1‑150 (MQ=255)
                aCGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAt                                                                                                                                   <  1:2896334/150‑1 (MQ=255)
                 cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATg                                                                                                                                  >  1:1565390/1‑150 (MQ=255)
                  gTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATgg                                                                                                                                 >  1:750376/1‑150 (MQ=255)
                        ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGt                                                                                                                           >  1:826305/1‑150 (MQ=255)
                          gcgTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCt                                                                                                                         <  1:3706145/150‑1 (MQ=255)
                          gcgTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCt                                                                                                                         <  2:5020786/150‑1 (MQ=255)
                           cgTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGGTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTg                                                                                                                        >  2:4586355/1‑150 (MQ=255)
                            gTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTgc                                                                                                                       >  2:208200/1‑150 (MQ=255)
                              aaTCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTgcgc                                                                                                                     <  2:816948/150‑1 (MQ=255)
                                  tGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATc                                                                                                                 <  2:3583808/150‑1 (MQ=255)
                                  tGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATc                                                                                                                 <  2:4842367/150‑1 (MQ=255)
                                  tGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATc                                                                                                                 >  1:1113822/1‑150 (MQ=255)
                                    aCGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                               >  2:364786/1‑150 (MQ=255)
                                      ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAc                                                                                                             >  1:5154989/1‑150 (MQ=255)
                                      ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAc                                                                                                             >  1:2626553/1‑150 (MQ=255)
                                      ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAc                                                                                                             >  2:1300914/1‑150 (MQ=255)
                                         gcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                          >  2:4860978/1‑150 (MQ=255)
                                         gcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                          <  2:768146/150‑1 (MQ=255)
                                             gCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATc                                                                                                      <  2:4692084/150‑1 (MQ=255)
                                               cAGTACTGAACCCACGCTGCAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                    >  2:4323227/1‑150 (MQ=255)
                                               cAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                    <  1:2285864/150‑1 (MQ=255)
                                                aGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                   >  2:2444253/1‑150 (MQ=255)
                                                 gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCta                                                                                                  <  1:1310814/150‑1 (MQ=255)
                                                  tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAACAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCtat                                                                                                 <  1:4398139/150‑1 (MQ=255)
                                                        aCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                           >  1:1050258/1‑150 (MQ=255)
                                                           taCGCAACAGGATCTGCTGGCGTTTCTTGACGAAGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGAGCGATCATACTGATATCGCTATAGATGACGa                                                                                        >  1:3579244/2‑150 (MQ=255)
                                                           cACTCAACAGTATCTGCTGGAGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAATCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGa                                                                                        <  2:2290921/150‑1 (MQ=255)
                                                           cACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGTGACTTATACTTGGGATGGTCTGCGCGATCATACTGATATCGCGATAGATGACGa                                                                                        >  2:2952274/1‑150 (MQ=255)
                                                            aCGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAt                                                                                       <  1:2965312/150‑1 (MQ=255)
                                                               cAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAtctc                                                                                    <  2:114103/150‑1 (MQ=255)
                                                                aaCAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAGAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATctct                                                                                   <  1:3262886/150‑1 (MQ=255)
                                                                aaCAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATctct                                                                                   <  1:1601882/150‑1 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACACTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                 <  2:3420369/150‑1 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                 >  2:4296546/1‑150 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                 <  2:5122438/150‑1 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                 >  2:438885/1‑150 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                 <  2:2825340/150‑1 (MQ=255)
                                                                   aGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGc                                                                                >  2:3605926/1‑150 (MQ=255)
                                                                   aGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGc                                                                                <  1:4837875/150‑1 (MQ=255)
                                                                   aGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGc                                                                                >  1:2439369/1‑150 (MQ=255)
                                                                      aTCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgtc                                                                             <  1:1977737/150‑1 (MQ=255)
                                                                         tgctgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGtt                                                                          >  1:1184989/1‑150 (MQ=255)
                                                                               cGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTa                                                                    >  2:1502826/1‑150 (MQ=255)
                                                                               cGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTa                                                                    >  1:5066864/1‑150 (MQ=255)
                                                                                     ttGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACa                                                              <  1:4065880/150‑1 (MQ=255)
                                                                                      tGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaa                                                             <  1:2600938/150‑1 (MQ=255)
                                                                                       gacgacGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaaa                                                            >  2:41072/1‑150 (MQ=255)
                                                                                        acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                           >  1:2222042/1‑150 (MQ=255)
                                                                                        acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                           >  1:4906204/1‑150 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCTAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         <  1:3977038/150‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCTCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         <  2:4838009/150‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         >  1:1246605/1‑150 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         >  2:2532590/1‑150 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         >  1:770916/1‑150 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGcc                                                         >  1:3355616/1‑150 (MQ=255)
                                                                                           acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCa                                                        <  1:4800635/150‑1 (MQ=255)
                                                                                           acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCa                                                        <  2:3285542/150‑1 (MQ=255)
                                                                                             gaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                      >  1:4966787/1‑150 (MQ=255)
                                                                                             gaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                      >  1:1931393/1‑150 (MQ=255)
                                                                                              aaCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCt                                                     <  2:2713625/150‑1 (MQ=255)
                                                                                              aaCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTATGGCGTCGTTTAAGTACCGACAAAGCCAGCt                                                     <  1:3828710/150‑1 (MQ=255)
                                                                                                  gACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCt                                                 <  1:4304066/150‑1 (MQ=255)
                                                                                                     gCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCaa                                              >  1:3473347/1‑150 (MQ=255)
                                                                                                       cGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAAcc                                            >  1:1479370/1‑150 (MQ=255)
                                                                                                         aaCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGc                                          <  1:3177036/150‑1 (MQ=255)
                                                                                                          aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCa                                         >  2:558341/1‑150 (MQ=255)
                                                                                                            aaTCATGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAt                                       <  1:2783081/150‑1 (MQ=255)
                                                                                                            aaTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAt                                       >  2:1801518/1‑150 (MQ=255)
                                                                                                             aTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAtt                                      >  1:2746284/1‑150 (MQ=255)
                                                                                                               cAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGACGTTTAAGTACCGACAAAGACAGCAGCCTCAACCGCAATTGt                                    >  1:930942/1‑150 (MQ=255)
                                                                                                                   aaGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtact                                <  1:5044652/150‑1 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtacta                               >  2:3970100/1‑150 (MQ=255)
                                                                                                                        cAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTAt                           <  2:2795076/150‑1 (MQ=255)
                                                                                                                         aaaaaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATc                          >  1:2115189/1‑150 (MQ=255)
                                                                                                                          aaaaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATCAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCg                         <  2:4021164/150‑1 (MQ=255)
                                                                                                                           aaaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                        >  2:1675159/1‑150 (MQ=255)
                                                                                                                              cGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCACTTGTTACTACTATCGTGaa                     >  1:4330123/1‑150 (MQ=255)
                                                                                                                                 tgtgCGAAGCTGAAGGGCTATCTCGCTACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAATTACCGACAAAGCACGCTGCCTCAACCGCAATTGTTACTAATATCGTGAATGc                  >  1:2514574/1‑150 (MQ=255)
                                                                                                                                     cGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCATCTGCCTCAACCGCAATTGTTACTACTATCGTGAAAGACCGt              >  2:2656688/1‑150 (MQ=255)
                                                                                                                                       aaGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGttt            >  2:1935778/1‑150 (MQ=255)
                                                                                                                                        aGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt           >  1:691051/1‑150 (MQ=255)
                                                                                                                                          cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttttt         <  2:3749209/150‑1 (MQ=255)
                                                                                                                                          cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttttt         <  1:509357/150‑1 (MQ=255)
                                                                                                                                          cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttttt         >  1:1200172/1‑150 (MQ=255)
                                                                                                                                            gAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACTGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGt       >  2:698206/1‑150 (MQ=255)
                                                                                                                                            gAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGt       >  2:1239149/1‑150 (MQ=255)
                                                                                                                                            gAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGt       <  2:1482390/150‑1 (MQ=255)
                                                                                                                                              aGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCg     <  2:3168289/150‑1 (MQ=255)
                                                                                                                                               gggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGc    <  1:3771902/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTC  >  CP009273/828862‑829156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑