Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 842,041 C→A 100% E179* (GAA→TAA)  glnP ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273842,0410CA98.9% 1058.9 / 0.1 331E179* (GAA→TAA) glnPglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (175/151);  total (177/154)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 331 total aligned reads displayed.

TGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAA  >  CP009273/841895‑842189
                                                                                                                                                  |                                                                                                                                                    
   agaCGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGccc                                                                                                                                                >  2:1564696/1‑150 (MQ=255)
   agaCGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGccc                                                                                                                                                >  2:4142161/1‑150 (MQ=255)
      cGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCACTAATTTATTGCCCCTg                                                                                                                                             >  2:2403951/1‑150 (MQ=255)
      cGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTg                                                                                                                                             >  2:780792/1‑150 (MQ=255)
       gTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACGAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGa                                                                                                                                            <  1:1353548/150‑1 (MQ=255)
       gTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGa                                                                                                                                            <  2:564116/150‑1 (MQ=255)
        tttttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGAc                                                                                                                                           <  2:5049110/150‑1 (MQ=255)
         ttttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACg                                                                                                                                          <  2:3866488/150‑1 (MQ=255)
          tttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACgg                                                                                                                                         <  2:542014/150‑1 (MQ=255)
             aaaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTc                                                                                                                                      >  1:4210099/1‑150 (MQ=255)
              aaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCa                                                                                                                                     >  1:327737/1‑150 (MQ=255)
              aaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCa                                                                                                                                     >  1:4966056/1‑150 (MQ=255)
              aaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCa                                                                                                                                     >  1:3066111/1‑150 (MQ=255)
               agtCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAg                                                                                                                                    <  2:4511291/148‑1 (MQ=255)
                 tCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGtt                                                                                                                                  <  1:4831474/150‑1 (MQ=255)
                   aaTCACAGGATTTTCATCCTTTTTTCCAGACGTCGCAGAATAAAGCTAAGCACCAGGTTAATATTCAGATAGAACTCCGCCACGGCGCTCCAGATCTCTATGTCGAGGAATTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTca                                                                                                                                <  1:2816823/150‑2 (MQ=255)
                    aTCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCg                                                                                                                               <  1:180552/150‑1 (MQ=255)
                      cacaGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGcc                                                                                                                             >  1:3189646/1‑150 (MQ=255)
                       acaGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCa                                                                                                                            <  1:4466639/150‑1 (MQ=255)
                        caGGATTTTCGTCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCAc                                                                                                                           <  2:2817712/150‑1 (MQ=255)
                        caGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCAc                                                                                                                           >  2:3047329/1‑150 (MQ=255)
                           gATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGcc                                                                                                                        >  1:2626734/1‑150 (MQ=255)
                           gATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGcc                                                                                                                        <  1:439614/150‑1 (MQ=255)
                              tttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGAt                                                                                                                     <  1:2149085/150‑1 (MQ=255)
                              tttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGAt                                                                                                                     <  1:3876545/150‑1 (MQ=255)
                              tttAATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATATTCAGATAGTACACCGCCACGGCGCTCCAGATCTCTATGGCGCGGAAGTTACCGGCAATAATTTATTGCCCGTGACGGGTCAGTTCCGCCAAGCCGAt                                                                                                                     <  1:3324584/150‑1 (MQ=255)
                                tCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATca                                                                                                                   <  1:5049848/150‑1 (MQ=255)
                                 cATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcac                                                                                                                  >  1:1619958/1‑150 (MQ=255)
                                 cATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcac                                                                                                                  <  1:5152801/150‑1 (MQ=255)
                                  aTCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcaca                                                                                                                 >  1:98635/1‑150 (MQ=255)
                                  aTCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcaca                                                                                                                 >  2:4328946/1‑150 (MQ=255)
                                      ttcttTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATaa                                                                                                             <  1:1606060/150‑1 (MQ=255)
                                      ttcttTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATaa                                                                                                             <  2:661339/150‑1 (MQ=255)
                                         tttCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACa                                                                                                          >  2:25928/1‑150 (MQ=255)
                                           tCCCGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGc                                                                                                        <  2:2689721/150‑1 (MQ=255)
                                              aGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAg                                                                                                     <  2:3572136/150‑1 (MQ=255)
                                              aGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAg                                                                                                     >  2:444810/1‑150 (MQ=255)
                                                acgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgt                                                                                                   <  2:2188845/150‑1 (MQ=255)
                                                acgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgt                                                                                                   >  2:4856029/1‑150 (MQ=255)
                                                 cgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgtg                                                                                                  >  2:1342107/1‑150 (MQ=255)
                                                 cgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgtg                                                                                                  >  2:495003/1‑150 (MQ=255)
                                                   acgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCTGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTc                                                                                                <  1:3594499/150‑1 (MQ=255)
                                                    cgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCt                                                                                               <  2:1092605/150‑1 (MQ=255)
                                                     gCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCtt                                                                                              >  2:1770000/1‑150 (MQ=255)
                                                     gCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTAtt                                                                                              <  2:3439335/150‑1 (MQ=255)
                                                       aGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTa                                                                                            >  2:1403063/1‑150 (MQ=255)
                                                          aTAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCCATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACCGCGAGGTGTCTTTAATg                                                                                         >  2:4396498/1‑150 (MQ=255)
                                                             aaGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtg                                                                                      >  1:333401/1‑150 (MQ=255)
                                                              aGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtga                                                                                     >  1:4916886/1‑150 (MQ=255)
                                                               gCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtgat                                                                                    <  2:1299119/150‑1 (MQ=255)
                                                                cTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGTCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtgatg                                                                                   <  1:4646692/150‑1 (MQ=255)
                                                                  cAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTgatgat                                                                                 >  2:376341/1‑150 (MQ=255)
                                                                      aCCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCAc                                                                             >  2:2081657/1‑150 (MQ=255)
                                                                         aGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTgg                                                                          <  2:3125622/150‑1 (MQ=255)
                                                                         aGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTgg                                                                          <  2:4704442/150‑1 (MQ=255)
                                                                            gTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTa                                                                       >  1:655534/1‑150 (MQ=255)
                                                                                taatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCa                                                                   <  2:2872703/150‑1 (MQ=255)
                                                                                taatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATGTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCa                                                                   >  2:1217925/1‑150 (MQ=255)
                                                                                 aatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAg                                                                  >  2:1952704/1‑150 (MQ=255)
                                                                                 aatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAg                                                                  >  2:3282845/1‑150 (MQ=255)
                                                                                    cAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAgcgg                                                               >  2:4201233/1‑150 (MQ=255)
                                                                                    cAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAgcgg                                                               >  1:1767955/1‑150 (MQ=255)
                                                                                      gATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAgcggcg                                                             <  1:4519310/150‑1 (MQ=255)
                                                                                      gATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAgcggcg                                                             >  2:2974794/1‑150 (MQ=255)
                                                                                        tataaCACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGATGATGATCCACTGGTTACCCAGCggcggc                                                           >  2:1621962/4‑150 (MQ=255)
                                                                                        tAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCggcggc                                                           >  2:2839183/1‑150 (MQ=255)
                                                                                          gAACACCGCCACGGCGCTCCAGATCTCAAGGTCGCGGAAGTTACCGGCAATAATTTATTTCCCATTACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGACAGGATTCTTTAATTCTGAAGATCCACTGGTTACCCAGCGGAGgcag                                                         >  1:3524309/1‑150 (MQ=255)
                                                                                          gAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCCGCGGCGgcag                                                         >  1:3893391/1‑150 (MQ=255)
                                                                                           aaCACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcagc                                                        >  1:1693499/1‑150 (MQ=255)
                                                                                            acacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCACTAATTGATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTCCCCCGCGGCGgcagcc                                                       >  2:4899102/1‑149 (MQ=255)
                                                                                            acacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACACTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcagca                                                       >  2:4505276/1‑150 (MQ=255)
                                                                                             cacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCAt                                                      >  1:1055468/1‑150 (MQ=255)
                                                                                              acCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATa                                                     >  1:1119724/1‑150 (MQ=255)
                                                                                                 gCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTGTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacga                                                  >  1:4751288/1‑150 (MQ=255)
                                                                                                  ccACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgac                                                 <  1:771496/150‑1 (MQ=255)
                                                                                                   cACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTAGTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgacg                                                <  2:3505949/150‑1 (MQ=255)
                                                                                                    aCGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGc                                               >  2:1669798/1‑150 (MQ=255)
                                                                                                      ggCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAg                                             >  2:2492826/1‑150 (MQ=255)
                                                                                                      ggCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAg                                             >  1:3135048/1‑150 (MQ=255)
                                                                                                       gcgcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGt                                            >  2:1383212/1‑150 (MQ=255)
                                                                                                         gcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGc                                          >  1:4585501/1‑150 (MQ=255)
                                                                                                         gcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGc                                          <  1:3833188/150‑1 (MQ=255)
                                                                                                              aGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCg                                     >  2:39678/1‑150 (MQ=255)
                                                                                                              aGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCg                                     >  2:22869/1‑150 (MQ=255)
                                                                                                                aTCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGt                                   <  1:3446152/150‑1 (MQ=255)
                                                                                                                  ctcAAGGGCGCGGAAGTTACCGGCAAGAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaa                                 <  2:27782/150‑1 (MQ=255)
                                                                                                                   tcAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaaa                                >  1:1315810/1‑150 (MQ=255)
                                                                                                                      tgggCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATg                             >  2:1851202/2‑150 (MQ=255)
                                                                                                                        ggCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGAc                           >  1:4368588/1‑150 (MQ=255)
                                                                                                                          cgcgGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGt                         <  2:968803/150‑1 (MQ=255)
                                                                                                                             ggAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGc                      >  2:2110416/1‑150 (MQ=255)
                                                                                                                             ggAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGc                      <  1:1194978/150‑1 (MQ=255)
                                                                                                                               aaGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGa                    >  1:2660656/1‑150 (MQ=255)
                                                                                                                                   tACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATgg                >  1:220712/1‑150 (MQ=255)
                                                                                                                                   tACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATgg                >  2:425394/1‑150 (MQ=255)
                                                                                                                                    aCCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGt               >  2:855402/1‑150 (MQ=255)
                                                                                                                                       ggCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTc            <  1:3750036/150‑1 (MQ=255)
                                                                                                                                          aataatTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCa         <  1:897708/150‑1 (MQ=255)
                                                                                                                                          aataatTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCa         <  1:255338/150‑1 (MQ=255)
                                                                                                                                           ataatTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCaa        <  1:2329959/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAA  >  CP009273/841895‑842189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑