Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,672 G→T 100% L42I (CTC→ATC)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,6720GT98.1% 871.8 / 4.5 279L42I (CTC→ATC) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (136/137);  total (138/141)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 290 total aligned reads displayed.

TCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882525‑882818
                                                                                                                                                   |                                                                                                                                                  
tcCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATaa                                                                                                                                                  >  1:1534713/1‑150 (MQ=255)
tcCCGATCAATGCACAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATaa                                                                                                                                                  <  1:2618730/150‑1 (MQ=255)
 cccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                 <  2:5001489/150‑1 (MQ=255)
 cccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                 <  2:2566009/150‑1 (MQ=255)
  ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGataata                                                                                                                                                <  1:4332597/150‑1 (MQ=255)
  ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGataata                                                                                                                                                <  1:1757817/150‑1 (MQ=255)
   cGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAatat                                                                                                                                               >  1:240823/1‑150 (MQ=255)
    gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                              >  1:3981732/1‑150 (MQ=255)
    gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                              >  2:4092126/1‑150 (MQ=255)
     aTCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcg                                                                                                                                             >  1:723935/1‑150 (MQ=255)
       cAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcgcg                                                                                                                                           >  1:916274/1‑150 (MQ=255)
           gcgcAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATa                                                                                                                                       <  2:3566001/150‑1 (MQ=255)
               aGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAg                                                                                                                                   <  2:2812946/150‑1 (MQ=255)
               aGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAg                                                                                                                                   <  2:2888250/150‑1 (MQ=255)
                     tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTAc                                                                                                                             <  2:3810806/150‑1 (MQ=255)
                          aCCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATCTCGCGGATAGCAGGCGTACGGGtt                                                                                                                        >  2:3740898/1‑150 (MQ=255)
                          aCCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGtt                                                                                                                        >  2:2534627/1‑150 (MQ=255)
                             aGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                     >  2:63883/1‑150 (MQ=255)
                                aTGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAg                                                                                                                  <  1:5843/150‑1 (MQ=255)
                                    cATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACg                                                                                                              >  2:103001/1‑150 (MQ=255)
                                    cATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACg                                                                                                              <  2:404125/150‑1 (MQ=255)
                                     aTTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATCGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                             >  1:3042375/1‑150 (MQ=255)
                                     aTTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                             >  1:4563200/1‑150 (MQ=255)
                                       tACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCa                                                                                                           >  1:3666975/1‑150 (MQ=255)
                                        aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAt                                                                                                          >  1:2947077/1‑150 (MQ=255)
                                          gtgtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAttc                                                                                                        >  1:2575785/1‑149 (MQ=255)
                                          gtgtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTa                                                                                                        <  2:2167378/150‑1 (MQ=255)
                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTcaca                                                                                                     >  1:1907172/1‑150 (MQ=255)
                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACa                                                                                                     <  2:4575941/150‑1 (MQ=255)
                                              cccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAg                                                                                                    <  1:3335663/150‑1 (MQ=255)
                                                 aaaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                 >  2:1265502/1‑150 (MQ=255)
                                                 aaaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                 <  2:5148972/150‑1 (MQ=255)
                                                  aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcat                                                                                                <  2:760490/150‑3 (MQ=255)
                                                  aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCt                                                                                                <  1:2334177/150‑1 (MQ=255)
                                                  aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCt                                                                                                <  2:1484242/150‑1 (MQ=255)
                                                   aGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTg                                                                                               >  1:1631388/1‑150 (MQ=255)
                                                    gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTgg                                                                                              <  1:685948/150‑1 (MQ=255)
                                                     cGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                             >  2:1732030/1‑150 (MQ=255)
                                                     cGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                             <  2:1459383/150‑1 (MQ=255)
                                                     cGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAGTAACAGGCCTGGc                                                                                             >  2:4239824/1‑150 (MQ=255)
                                                       tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaa                                                                                           <  2:1741338/150‑1 (MQ=255)
                                                         tCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaa                                                                                         >  1:4232033/1‑150 (MQ=255)
                                                          cACTTACCACGCAGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACAAAAGAGAACACCGCCCATTTCAGAGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGTTTGCCCAGGACGCCATTAACAGGCCTGGCtaaaa                                                                                        <  1:2510036/150‑1 (MQ=255)
                                                          cACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaaa                                                                                        >  2:292743/1‑150 (MQ=255)
                                                              aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                    <  2:987951/150‑1 (MQ=255)
                                                              aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                    <  1:811917/150‑1 (MQ=255)
                                                               aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAagaaga                                                                                   <  2:3069580/150‑1 (MQ=255)
                                                                  aCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACa                                                                                <  2:4636906/150‑1 (MQ=255)
                                                                     ccGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATCGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaa                                                                             >  2:1335253/1‑150 (MQ=255)
                                                                       gAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                           >  1:1011955/1‑150 (MQ=255)
                                                                        aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACa                                                                          <  2:4897847/150‑1 (MQ=255)
                                                                        aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACa                                                                          >  2:5107718/1‑150 (MQ=255)
                                                                         gCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAg                                                                         <  1:1953017/150‑1 (MQ=255)
                                                                          cAGAGAATACCTCTCATCGAACCTATCGACAGACCAAAGAGAACACCGACCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCATGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAgc                                                                        <  1:1197563/150‑1 (MQ=255)
                                                                              gaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGccc                                                                    >  1:5082870/1‑150 (MQ=255)
                                                                              gaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGccc                                                                    <  1:1832079/150‑1 (MQ=255)
                                                                              gaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGccc                                                                    <  1:693767/150‑1 (MQ=255)
                                                                               aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCa                                                                   <  2:3238726/150‑1 (MQ=255)
                                                                                   ccGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                               <  1:4065141/150‑1 (MQ=255)
                                                                                    cGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                              <  1:2144126/150‑1 (MQ=255)
                                                                                     gCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCg                                                             >  2:3987715/1‑150 (MQ=255)
                                                                                      cTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCgg                                                            <  2:2873079/150‑1 (MQ=255)
                                                                                      cTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATGTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCgg                                                            <  2:263625/150‑1 (MQ=255)
                                                                                        cATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                          >  1:2731132/1‑150 (MQ=255)
                                                                                        cATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                          >  2:369015/1‑150 (MQ=255)
                                                                                        cATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                          <  1:1685344/150‑1 (MQ=255)
                                                                                         aTCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGt                                                         >  1:3468629/1‑150 (MQ=255)
                                                                                           cGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGttt                                                       <  2:1942183/150‑1 (MQ=255)
                                                                                           cGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGttt                                                       <  2:2403468/150‑1 (MQ=255)
                                                                                              aCCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCaa                                                    >  1:3592604/1‑150 (MQ=255)
                                                                                                cGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATg                                                  <  2:2311042/150‑1 (MQ=255)
                                                                                                  aTCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCa                                                <  1:4639300/150‑1 (MQ=255)
                                                                                                      aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTAc                                            >  1:2262011/1‑150 (MQ=255)
                                                                                                      aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTAc                                            <  2:3529567/150‑1 (MQ=255)
                                                                                                       cAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACg                                           >  1:3147390/1‑150 (MQ=255)
                                                                                                           ccAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaa                                       >  2:4417222/1‑150 (MQ=255)
                                                                                                                gagaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                  <  1:3725732/150‑1 (MQ=255)
                                                                                                                gagaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                  <  2:3177819/150‑1 (MQ=255)
                                                                                                                   aaCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                               <  2:4523833/150‑1 (MQ=255)
                                                                                                                   aaCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                               >  1:4900870/1‑150 (MQ=255)
                                                                                                                     cacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                             >  2:3374035/1‑150 (MQ=255)
                                                                                                                      acCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGt                            >  2:4820032/1‑150 (MQ=255)
                                                                                                                      acCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGt                            <  1:3837650/150‑1 (MQ=255)
                                                                                                                       ccGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTc                           <  1:3870342/150‑1 (MQ=255)
                                                                                                                         gCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                         <  2:3920073/150‑1 (MQ=255)
                                                                                                                         gCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                         >  1:4418630/1‑150 (MQ=255)
                                                                                                                          cccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATa                        <  1:4948674/150‑1 (MQ=255)
                                                                                                                             aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                     >  1:3698821/1‑150 (MQ=255)
                                                                                                                               ttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCa                   >  1:260403/1‑150 (MQ=255)
                                                                                                                                tCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCAc                  <  1:2500572/150‑1 (MQ=255)
                                                                                                                                    cGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCa              >  1:4756637/1‑150 (MQ=255)
                                                                                                                                        cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          <  2:1795306/150‑1 (MQ=255)
                                                                                                                                            aCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaaga      >  1:4110586/1‑150 (MQ=255)
                                                                                                                                            aCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaaga      <  2:1559247/150‑1 (MQ=255)
                                                                                                                                             cAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaa     <  1:5020432/150‑1 (MQ=255)
                                                                                                                                             cAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaa     <  2:460334/150‑1 (MQ=255)
                                                                                                                                             cAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaa     <  1:3558494/150‑1 (MQ=255)
                                                                                                                                              agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag    <  2:1011955/150‑1 (MQ=255)
                                                                                                                                               gagataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAagaaga   <  1:101302/150‑1 (MQ=255)
                                                                                                                                                agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAagag  <  1:300071/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882525‑882818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑