Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 925,160 G→T 100% S420* (TCA→TAA)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273925,1600GT99.7% 987.2 / ‑4.4 296S420* (TCA→TAA) cydDfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (154/141);  total (155/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 296 total aligned reads displayed.

GGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTA  >  CP009273/925013‑925306
                                                                                                                                                   |                                                                                                                                                  
    gAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTg                                                                                                                                              >  2:1930269/1‑150 (MQ=255)
     aaaCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTgg                                                                                                                                             >  2:1214437/1‑150 (MQ=255)
     aaaCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTgg                                                                                                                                             >  2:354453/1‑150 (MQ=255)
       aCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTg                                                                                                                                           <  1:1730160/150‑1 (MQ=255)
          cGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGaga                                                                                                                                        >  1:3044832/1‑148 (MQ=255)
          cGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATa                                                                                                                                        <  2:1421152/150‑1 (MQ=255)
          cGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATa                                                                                                                                        <  2:3364846/150‑1 (MQ=255)
            cTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATaaa                                                                                                                                      <  2:630558/150‑1 (MQ=255)
             tGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAAt                                                                                                                                     <  2:4527950/150‑1 (MQ=255)
             tGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAAt                                                                                                                                     >  2:2263155/1‑150 (MQ=255)
              gACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATc                                                                                                                                    >  2:1814094/1‑150 (MQ=255)
                    ggCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCACTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTaa                                                                                                                              <  2:3075819/150‑1 (MQ=255)
                      cGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAAtt                                                                                                                            >  1:3107756/1‑150 (MQ=255)
                       gTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTc                                                                                                                           >  2:4151789/1‑150 (MQ=255)
                        ttATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCt                                                                                                                          >  1:3573242/1‑150 (MQ=255)
                         tATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTa                                                                                                                         <  1:1300807/150‑1 (MQ=255)
                             cAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATccc                                                                                                                     <  1:529089/150‑1 (MQ=255)
                              aGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCg                                                                                                                    <  1:2316758/150‑1 (MQ=255)
                                cgcTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGtt                                                                                                                  <  1:1200860/150‑1 (MQ=255)
                                 gctgctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTg                                                                                                                 >  1:1143327/1‑150 (MQ=255)
                                  ctgctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTCATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGa                                                                                                                >  2:1637511/1‑150 (MQ=255)
                                  ctgctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCAAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGa                                                                                                                <  1:2739673/150‑1 (MQ=255)
                                    gctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTCTCCCGTTGAtt                                                                                                              >  1:3464004/1‑150 (MQ=255)
                                      ttGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCg                                                                                                            >  2:1850471/1‑150 (MQ=255)
                                       tGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGt                                                                                                           >  1:2412281/1‑150 (MQ=255)
                                          aaTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGc                                                                                                        <  1:1977351/150‑1 (MQ=255)
                                           agtCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCg                                                                                                       <  2:3037565/148‑1 (MQ=255)
                                             tCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCACTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTAGTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGAt                                                                                                     <  1:3378951/150‑1 (MQ=255)
                                             tCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGAt                                                                                                     <  1:3601086/150‑1 (MQ=255)
                                             tCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGAt                                                                                                     <  2:2650389/150‑1 (MQ=255)
                                              cTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATc                                                                                                    <  1:4611450/150‑1 (MQ=255)
                                              cTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGATGATTCGTAGCGATc                                                                                                    >  1:4971900/1‑150 (MQ=255)
                                               ttGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATcc                                                                                                   >  1:2824287/1‑150 (MQ=255)
                                                tGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATccc                                                                                                  >  2:776730/1‑150 (MQ=255)
                                                 gTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCt                                                                                                 <  2:3884719/150‑1 (MQ=255)
                                                 gTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCt                                                                                                 >  1:2522028/1‑150 (MQ=255)
                                                 gTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCt                                                                                                 <  2:5101951/150‑1 (MQ=255)
                                                  ttCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTg                                                                                                >  1:2121385/1‑150 (MQ=255)
                                                    cGCTGGCATCAGGTCGCGCCAGTAGTACGGTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGat                                                                                              <  1:4164739/150‑1 (MQ=255)
                                                      cTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGatat                                                                                            <  1:4463132/150‑1 (MQ=255)
                                                        ggCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATga                                                                                          <  2:3549386/150‑1 (MQ=255)
                                                         gCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATgag                                                                                         <  1:876398/150‑1 (MQ=255)
                                                          cATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATgaga                                                                                        <  2:869290/150‑1 (MQ=255)
                                                              aGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaaa                                                                                    >  1:2136996/1‑150 (MQ=255)
                                                              aGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaaa                                                                                    >  2:644704/1‑150 (MQ=255)
                                                               ggTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGacaa                                                                                   >  2:4937344/1‑150 (MQ=255)
                                                                  cgcgCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCa                                                                                >  1:2455578/1‑150 (MQ=255)
                                                                    cgcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGa                                                                              <  2:1394015/150‑1 (MQ=255)
                                                                     gcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGaa                                                                             <  1:4042203/150‑1 (MQ=255)
                                                                      ccAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGaaa                                                                            <  2:4287187/150‑1 (MQ=255)
                                                                       cAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAg                                                                           <  1:4716728/150‑1 (MQ=255)
                                                                       cAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAg                                                                           <  1:4762707/150‑1 (MQ=255)
                                                                         gtagtaCGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAgcg                                                                         <  1:3927255/150‑1 (MQ=255)
                                                                             taCGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGtt                                                                     >  2:4453762/1‑150 (MQ=255)
                                                                              aCGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTTATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTc                                                                    <  2:2330359/150‑1 (MQ=255)
                                                                               cGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTca                                                                   <  2:277186/150‑1 (MQ=255)
                                                                                gTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTcag                                                                  >  1:2774688/1‑150 (MQ=255)
                                                                                 ttATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTcagc                                                                 >  2:4620924/1‑150 (MQ=255)
                                                                                  tATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTcagca                                                                >  2:3936325/1‑150 (MQ=255)
                                                                                       ctcAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAg                                                           >  2:3772317/3‑150 (MQ=255)
                                                                                       cGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAg                                                           <  1:1695313/150‑1 (MQ=255)
                                                                                       cGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAg                                                           <  2:1956620/150‑1 (MQ=255)
                                                                                        gCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGc                                                          >  1:707603/1‑150 (MQ=255)
                                                                                         cAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCt                                                         >  2:4495589/1‑150 (MQ=255)
                                                                                         cAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCt                                                         >  2:1667345/1‑150 (MQ=255)
                                                                                         ataTGTTGCTGCGGGTAATTGTGGGTTTTGCCCAACACAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCAGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGGTCAGCAGTGAGCt                                                         <  1:5078248/148‑1 (MQ=255)
                                                                                          aaTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCtt                                                        >  2:2938704/1‑150 (MQ=255)
                                                                                            tGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCtttt                                                      >  2:3142085/1‑150 (MQ=255)
                                                                                            tGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCtttt                                                      >  2:3472995/1‑150 (MQ=255)
                                                                                             gTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTa                                                     <  1:1437991/150‑1 (MQ=255)
                                                                                             gTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTa                                                     >  2:496997/1‑150 (MQ=255)
                                                                                               tgctgcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTAcc                                                   <  2:2439484/150‑1 (MQ=255)
                                                                                                   gcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGaa                                               <  2:3402668/150‑1 (MQ=255)
                                                                                                   gcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGaa                                               <  2:1030259/150‑1 (MQ=255)
                                                                                                    ccGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAAc                                              >  1:1084581/1‑150 (MQ=255)
                                                                                                        tAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCt                                          >  2:2577529/1‑150 (MQ=255)
                                                                                                         aaTTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTg                                         >  2:467716/1‑150 (MQ=255)
                                                                                                          aTTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGc                                        <  1:610816/150‑1 (MQ=255)
                                                                                                           ttGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCg                                       <  2:4972532/150‑1 (MQ=255)
                                                                                                            tgtgGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGa                                      <  1:4856527/150‑1 (MQ=255)
                                                                                                                 gTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGaccaac                                 >  2:4871343/1‑150 (MQ=255)
                                                                                                                    ttGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGAccaaccaa                              <  2:3985438/150‑1 (MQ=255)
                                                                                                                     tGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACcaaccaac                             >  1:3162982/1‑150 (MQ=255)
                                                                                                                            cccAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCAc                      <  2:1084581/150‑1 (MQ=255)
                                                                                                                             ccAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACg                     >  1:4309292/1‑150 (MQ=255)
                                                                                                                               aGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGtt                   >  1:4341006/1‑150 (MQ=255)
                                                                                                                               aGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGtt                   <  1:1850471/150‑1 (MQ=255)
                                                                                                                                ggAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTg                  >  2:3876264/1‑150 (MQ=255)
                                                                                                                                ggAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTg                  <  1:776730/150‑1 (MQ=255)
                                                                                                                                     gaTGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctg             >  2:2403751/1‑150 (MQ=255)
                                                                                                                                      aTGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgc            >  2:40142/1‑150 (MQ=255)
                                                                                                                                       tGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgct           <  1:3217539/150‑1 (MQ=255)
                                                                                                                                             cGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaa     <  2:4917310/150‑1 (MQ=255)
                                                                                                                                              gCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaag    >  2:3265256/1‑150 (MQ=255)
                                                                                                                                              gCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaag    >  2:81892/1‑150 (MQ=255)
                                                                                                                                               ccATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagt   >  1:1629036/1‑150 (MQ=255)
                                                                                                                                                cATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagta  >  2:4168631/1‑150 (MQ=255)
                                                                                                                                                cATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagta  >  2:5043983/1‑150 (MQ=255)
                                                                                                                                                cATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagta  >  2:3636536/1‑150 (MQ=255)
                                                                                                                                                cATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagta  >  1:2878741/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTA  >  CP009273/925013‑925306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑