Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 943,573 G→T 100% A297S (GCC→TCC)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273943,5730GT100.0% 810.0 / NA 240A297S (GCC→TCC) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (114/126);  total (114/126)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

GTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAT  >  CP009273/943426‑943719
                                                                                                                                                   |                                                                                                                                                  
gTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTcc                                                                                                                                                  <  1:1611492/150‑1 (MQ=255)
 tttGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCa                                                                                                                                                 <  2:3084311/150‑1 (MQ=255)
     tgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGc                                                                                                                                             <  2:2654866/150‑1 (MQ=255)
         aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAt                                                                                                                                         <  1:2219382/150‑1 (MQ=255)
         aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAt                                                                                                                                         <  1:650840/150‑1 (MQ=255)
           tCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAc                                                                                                                                       >  2:2738720/1‑150 (MQ=255)
           tCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAc                                                                                                                                       <  2:4122355/150‑1 (MQ=255)
             tGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAccc                                                                                                                                     <  2:2179629/150‑1 (MQ=255)
             tGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAccc                                                                                                                                     >  2:755862/1‑150 (MQ=255)
              gggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCt                                                                                                                                    >  1:2955333/1‑150 (MQ=255)
                gCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTgg                                                                                                                                  <  2:1926193/150‑1 (MQ=255)
                gCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTgg                                                                                                                                  <  2:4497005/150‑1 (MQ=255)
                 cTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTggg                                                                                                                                 <  1:1774285/150‑1 (MQ=255)
                 cTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTggg                                                                                                                                 <  2:4303255/150‑1 (MQ=255)
                 cTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTggg                                                                                                                                 <  1:2175608/150‑1 (MQ=255)
                  tagCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGATGGGCGAGTATTACAACATGGGTAATACTATAATGGTGATTTACTCCATTGCGAATACCCTGGGa                                                                                                                                >  1:4269183/3‑150 (MQ=255)
                  tCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGa                                                                                                                                <  2:3052864/150‑1 (MQ=255)
                  tCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGa                                                                                                                                <  1:4168330/150‑1 (MQ=255)
                  tCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGa                                                                                                                                <  1:3452782/150‑1 (MQ=255)
                    gCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACa                                                                                                                              <  1:4941319/150‑1 (MQ=255)
                    gCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACa                                                                                                                              >  2:3094354/1‑150 (MQ=255)
                     cttgCGATGGTGATGATGATTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTTTTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGTTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                             <  2:2203086/147‑1 (MQ=255)
                         cGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAg                                                                                                                         >  1:1428031/1‑150 (MQ=255)
                          gATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgc                                                                                                                        >  2:3727357/1‑150 (MQ=255)
                           aTGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcg                                                                                                                       >  1:3492563/1‑150 (MQ=255)
                            tGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcgg                                                                                                                      >  2:1878116/1‑150 (MQ=255)
                            tGGGGATGATGTTTGATTCGCGTAATATCACGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcgg                                                                                                                      >  2:3539810/1‑150 (MQ=255)
                             ggggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggc                                                                                                                     <  1:937340/150‑1 (MQ=255)
                                gatgatGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCt                                                                                                                  <  2:4188767/150‑1 (MQ=255)
                                 atgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTg                                                                                                                 <  1:4872419/150‑1 (MQ=255)
                                    atgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTa                                                                                                              <  1:2756071/150‑1 (MQ=255)
                                      gTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                            <  1:982004/150‑1 (MQ=255)
                                       tttGATTCGCGTAATATGCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtc                                                                                                           <  2:4259519/150‑1 (MQ=255)
                                       tttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtc                                                                                                           <  2:708492/150‑1 (MQ=255)
                                        ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtct                                                                                                          >  2:117284/1‑150 (MQ=255)
                                         tGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                         <  2:210730/150‑1 (MQ=255)
                                           aTTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                       <  1:1821363/150‑1 (MQ=255)
                                             tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGAtt                                                                                                     <  2:5052133/150‑1 (MQ=255)
                                                cgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGAt                                                                                                  >  2:1712768/1‑150 (MQ=255)
                                                 gTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                                 >  2:1040391/1‑150 (MQ=255)
                                                  tAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGc                                                                                                <  1:5048969/150‑1 (MQ=255)
                                                   aaTATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGcc                                                                                               <  1:1722836/150‑1 (MQ=255)
                                                      atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                            <  1:506965/150‑1 (MQ=255)
                                                      atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                            <  1:1199710/150‑1 (MQ=255)
                                                      atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                            <  1:1977464/150‑1 (MQ=255)
                                                         ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                         >  2:525135/1‑150 (MQ=255)
                                                          cGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTa                                                                                        <  2:3705855/150‑1 (MQ=255)
                                                           ggATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaa                                                                                       >  2:4254620/1‑150 (MQ=255)
                                                            gATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaaa                                                                                      >  2:4986753/1‑150 (MQ=255)
                                                            gATGACATAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaaa                                                                                      <  2:4666785/150‑1 (MQ=255)
                                                                 cTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCt                                                                                 <  2:5022623/150‑1 (MQ=255)
                                                                 cTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCt                                                                                 >  1:4840550/1‑150 (MQ=255)
                                                                   tAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAt                                                                               >  1:4255485/1‑150 (MQ=255)
                                                                    aaTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                              >  1:4538410/1‑150 (MQ=255)
                                                                       gACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAgg                                                                           >  1:201091/1‑150 (MQ=255)
                                                                          cAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGa                                                                        <  2:394534/150‑1 (MQ=255)
                                                                          cAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGa                                                                        <  2:4471091/150‑1 (MQ=255)
                                                                           aaCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTAAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGAt                                                                       >  1:387741/1‑150 (MQ=255)
                                                                              ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                    >  1:2412737/1‑150 (MQ=255)
                                                                               gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTg                                                                   <  2:3865845/150‑1 (MQ=255)
                                                                                 cAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGAc                                                                 <  2:1649921/150‑1 (MQ=255)
                                                                                     aTTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATAGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCa                                                             >  2:449579/1‑150 (MQ=255)
                                                                                       tACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaaa                                                           >  1:3096303/1‑150 (MQ=255)
                                                                                         tgCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCACata                                                         >  2:1453200/2‑150 (MQ=255)
                                                                                         cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAata                                                         >  1:592503/1‑150 (MQ=255)
                                                                                          gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatat                                                        <  2:3609136/150‑1 (MQ=255)
                                                                                              ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTc                                                    <  2:331992/150‑1 (MQ=255)
                                                                                               tCAGAAGCTGGGCGAGTATTACAACAGGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcc                                                   <  2:2274730/150‑1 (MQ=255)
                                                                                                cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                  >  2:3910568/1‑150 (MQ=255)
                                                                                                 aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                 >  2:2808993/1‑150 (MQ=255)
                                                                                                 aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                 <  2:677714/150‑1 (MQ=255)
                                                                                                 aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                 >  1:730709/1‑150 (MQ=255)
                                                                                                  gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagc                                                >  1:2796996/1‑150 (MQ=255)
                                                                                                  gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagc                                                >  1:3687369/1‑150 (MQ=255)
                                                                                                  gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGAGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTTATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATAATccagc                                                >  1:1140487/1‑150 (MQ=255)
                                                                                                   aaGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcc                                               >  1:63484/1‑150 (MQ=255)
                                                                                                      cTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGt                                            >  1:3178667/1‑150 (MQ=255)
                                                                                                         ggCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTa                                         <  1:4721498/150‑1 (MQ=255)
                                                                                                         ggCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTa                                         >  1:1608979/1‑150 (MQ=255)
                                                                                                         ggCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTa                                         <  2:3989357/150‑1 (MQ=255)
                                                                                                          gCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTAt                                        <  1:2305377/150‑1 (MQ=255)
                                                                                                           cGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATg                                       >  1:2615489/1‑150 (MQ=255)
                                                                                                            gAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGt                                      <  1:1296824/150‑1 (MQ=255)
                                                                                                             aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTc                                     <  2:234703/150‑1 (MQ=255)
                                                                                                               tATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGt                                   >  2:3187155/1‑150 (MQ=255)
                                                                                                                 ttAAAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACTAGTAGCGGCGCTGGTTTTCTCGATTTATGCACCGCTTAAAGTTCTATTAGTTGATGATGACAGCAAATATATTCCAGCCTGTTTATGTCGTAc                                 <  2:4857172/150‑1 (MQ=255)
                                                                                                                  tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTAcc                                >  2:2991755/1‑150 (MQ=255)
                                                                                                                   acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCACTCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGGCAGTTTATTTCTTAccc                               >  2:5073263/1‑149 (MQ=255)
                                                                                                                    caacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCaa                              <  2:41776/150‑1 (MQ=255)
                                                                                                                     aacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAAc                             >  2:825275/1‑150 (MQ=255)
                                                                                                                          gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                        >  2:3564570/1‑150 (MQ=255)
                                                                                                                          gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                        >  1:665980/1‑150 (MQ=255)
                                                                                                                           ggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                       >  1:366994/1‑150 (MQ=255)
                                                                                                                           ggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                       >  1:4614439/1‑150 (MQ=255)
                                                                                                                                     tAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCg             >  1:4689863/1‑150 (MQ=255)
                                                                                                                                       aTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGtt           <  2:1209673/150‑1 (MQ=255)
                                                                                                                                              tttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCt    >  2:2380371/1‑150 (MQ=255)
                                                                                                                                               ttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTa   >  2:571808/1‑150 (MQ=255)
                                                                                                                                                tACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAt  >  1:1853249/1‑150 (MQ=255)
                                                                                                                                                tACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAt  >  1:1432644/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAT  >  CP009273/943426‑943719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑