Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 968,415 G→T 100% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273968,4150GT100.0% 1043.2 / ‑6.7 317S148* (TCG→TAG) ycbCDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (146/170);  minor base A (1/0);  total (147/170)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

AAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATT  >  CP009273/968269‑968560
                                                                                                                                                  |                                                                                                                                                 
aaagGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                <  1:1297453/150‑1 (MQ=255)
aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCATCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                >  1:2725868/1‑150 (MQ=255)
aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                <  2:3374621/150‑1 (MQ=255)
   aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcg                                                                                                                                             >  2:1506416/1‑150 (MQ=255)
    gATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcgc                                                                                                                                            <  1:4740296/150‑1 (MQ=255)
    gATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcgc                                                                                                                                            <  1:4417324/150‑1 (MQ=255)
      tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCaa                                                                                                                                          >  2:4936260/1‑150 (MQ=255)
       catcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAc                                                                                                                                         >  2:727858/1‑150 (MQ=255)
        atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATACTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAct                                                                                                                                        >  2:4782079/1‑150 (MQ=255)
         tcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAActc                                                                                                                                       <  2:4594062/150‑1 (MQ=255)
          cGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAActct                                                                                                                                      <  1:1724470/150‑1 (MQ=255)
          cGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAActct                                                                                                                                      <  2:1059947/150‑1 (MQ=255)
           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTg                                                                                                                                     <  2:138161/150‑1 (MQ=255)
           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTg                                                                                                                                     <  2:3494207/150‑1 (MQ=255)
           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGACACGCCCAGCTATTGCGCAACTCTg                                                                                                                                     >  1:3273033/1‑150 (MQ=255)
            cccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTgg                                                                                                                                    >  1:5018226/1‑150 (MQ=255)
              cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCa                                                                                                                                  <  2:103222/150‑1 (MQ=255)
               gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCAc                                                                                                                                 <  1:1540728/150‑1 (MQ=255)
                cgGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCAcc                                                                                                                                >  1:2095574/1‑150 (MQ=255)
                 ggCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCt                                                                                                                               <  2:2248209/150‑1 (MQ=255)
                 ggCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCt                                                                                                                               >  2:3944352/1‑150 (MQ=255)
                    aGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACt                                                                                                                            >  2:2446222/1‑150 (MQ=255)
                    aGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACt                                                                                                                            <  2:1106864/150‑1 (MQ=255)
                    aGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACt                                                                                                                            >  2:3269190/1‑150 (MQ=255)
                       ggtgcggcggcgGTCACCCGCAGGCACGGCGCGTCACCACTCGCCTGTTTCACTGCTGCAGCGTCTTCTACGGTATCTTTTGGCAACGCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTcc                                                                                                                         <  1:2619808/144‑1 (MQ=255)
                          gATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCt                                                                                                                      >  2:3849273/1‑150 (MQ=255)
                           aTGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTg                                                                                                                     <  2:358341/150‑1 (MQ=255)
                                gAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTc                                                                                                                >  2:5155125/1‑150 (MQ=255)
                                   gTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCAcc                                                                                                             >  2:2584354/1‑150 (MQ=255)
                                       ccAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAt                                                                                                         <  2:158212/150‑1 (MQ=255)
                                         agcagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTg                                                                                                       >  1:2413955/1‑150 (MQ=255)
                                            agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtt                                                                                                    >  1:4565055/1‑150 (MQ=255)
                                            agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGGATTGGtt                                                                                                    >  2:836381/1‑150 (MQ=255)
                                             ggAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttt                                                                                                   >  1:3537165/1‑150 (MQ=255)
                                             ggAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttt                                                                                                   >  2:1185125/1‑150 (MQ=255)
                                              gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                  <  2:3597875/150‑1 (MQ=255)
                                               aaaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttttt                                                                                                 <  1:3051274/150‑1 (MQ=255)
                                                 aGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGc                                                                                               >  2:1263715/1‑150 (MQ=255)
                                                  ggggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCt                                                                                              <  1:408740/150‑1 (MQ=255)
                                                  ggggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCt                                                                                              <  2:144665/150‑1 (MQ=255)
                                                   gggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTa                                                                                             <  2:5050032/150‑1 (MQ=255)
                                                       gTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGcc                                                                                         >  1:949920/1‑150 (MQ=255)
                                                       gTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGcc                                                                                         >  1:2937287/1‑150 (MQ=255)
                                                        tCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCt                                                                                        >  2:3251004/1‑150 (MQ=255)
                                                         cACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTc                                                                                       >  1:1067023/1‑150 (MQ=255)
                                                           ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGNTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                     >  1:2678024/1‑150 (MQ=255)
                                                             aaTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGt                                                                                   <  2:3898756/150‑1 (MQ=255)
                                                             aaTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGt                                                                                   >  2:2744964/1‑150 (MQ=255)
                                                              aTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTg                                                                                  <  2:5022975/150‑1 (MQ=255)
                                                                cGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGaa                                                                                <  1:2390016/150‑1 (MQ=255)
                                                                cGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGaa                                                                                >  1:2772112/1‑150 (MQ=255)
                                                                      tttCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAg                                                                          <  1:1585402/150‑1 (MQ=255)
                                                                       ttCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGt                                                                         >  1:512588/1‑150 (MQ=255)
                                                                       ttCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGt                                                                         <  1:4331283/150‑1 (MQ=255)
                                                                          aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                      <  1:3232401/150‑1 (MQ=255)
                                                                           ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTg                                                                     <  1:2466239/150‑1 (MQ=255)
                                                                            tgctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGa                                                                    >  2:4997251/1‑150 (MQ=255)
                                                                            tgctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGa                                                                    >  1:1345707/1‑150 (MQ=255)
                                                                               tgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATcc                                                                 <  1:4035208/150‑1 (MQ=255)
                                                                                 cAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCg                                                               <  1:1093504/150‑1 (MQ=255)
                                                                                  aGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                              <  1:558387/150‑1 (MQ=255)
                                                                                   gCTTCTACTTCGGTCTCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACTCCCACCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCAACGTATTGGTTTATGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGa                                                             >  2:4677274/1‑150 (MQ=255)
                                                                                          cttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgc                                                      >  2:2171472/1‑150 (MQ=255)
                                                                                          cttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgc                                                      <  2:2377104/150‑1 (MQ=255)
                                                                                             cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGcc                                                   >  1:1007592/1‑150 (MQ=255)
                                                                                                 aTCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATaa                                               <  2:487499/150‑1 (MQ=255)
                                                                                                 aTCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATaa                                               <  1:4127730/150‑1 (MQ=255)
                                                                                                 aTCTTGTGGCAAATCCAGGGTGAGAATGTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCGGGCACCTACTTCCGCTGGACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATaa                                               <  2:2619671/150‑1 (MQ=255)
                                                                                                  tCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAg                                              <  1:1481693/150‑1 (MQ=255)
                                                                                                    ttttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCACGGATTTTCGCGCCATAAGCg                                            >  1:1903900/1‑150 (MQ=255)
                                                                                                     tttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGa                                           <  1:3926288/150‑1 (MQ=255)
                                                                                                       tGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                         >  2:2312727/1‑150 (MQ=255)
                                                                                                        ggCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATa                                        >  1:1256610/1‑150 (MQ=255)
                                                                                                        ggCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATa                                        <  1:1429696/150‑1 (MQ=255)
                                                                                                           aaaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                     >  2:4300520/1‑150 (MQ=255)
                                                                                                           aaaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                     <  2:3438579/150‑1 (MQ=255)
                                                                                                              tCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTGCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttca                                  <  2:4142373/150‑1 (MQ=255)
                                                                                                                   ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAg                             >  1:2256170/1‑150 (MQ=255)
                                                                                                                     tGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGc                           <  1:3297223/150‑1 (MQ=255)
                                                                                                                     tGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGc                           >  2:3691927/1‑150 (MQ=255)
                                                                                                                      gATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCg                          >  2:2506843/1‑150 (MQ=255)
                                                                                                                       aTAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGt                         >  2:1780558/1‑150 (MQ=255)
                                                                                                                        tAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTg                        <  1:514360/150‑1 (MQ=255)
                                                                                                                        tAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTg                        <  1:4290534/150‑1 (MQ=255)
                                                                                                                         aaTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGACGATCCGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCTTTCAGGCGTgg                       >  2:2943286/1‑150 (MQ=255)
                                                                                                                         aaTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTgg                       <  1:341626/150‑1 (MQ=255)
                                                                                                                         aaTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTgg                       <  1:1240648/150‑1 (MQ=255)
                                                                                                                          aTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGc                      <  2:4428331/150‑1 (MQ=255)
                                                                                                                           tttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCa                     >  2:944808/1‑150 (MQ=255)
                                                                                                                           tttGCTCGCGCGACACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCa                     <  2:3273033/150‑1 (MQ=255)
                                                                                                                                 cgcgcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGt               >  1:4668078/1‑150 (MQ=255)
                                                                                                                                    gcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTAt            <  1:4376254/150‑1 (MQ=255)
                                                                                                                                     gggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTAtt           >  2:574553/2‑150 (MQ=255)
                                                                                                                                       tcACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGa         >  2:1777589/2‑150 (MQ=255)
                                                                                                                                       gCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGa         <  1:3944352/150‑1 (MQ=255)
                                                                                                                                         aCGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGAtt       <  2:1994708/150‑1 (MQ=255)
                                                                                                                                         aCGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAATGGTTGTTGCTACGCCTCCCGAGAAGATCAGATTTGATCCCGTATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGAtt       >  1:3369948/1‑150 (MQ=255)
                                                                                                                                           gCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaa     >  2:2049967/1‑150 (MQ=255)
                                                                                                                                            cccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaaa    >  1:4912032/1‑150 (MQ=255)
                                                                                                                                              cAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAAtt  >  2:3143122/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATT  >  CP009273/968269‑968560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑