Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 997,673 A→T 100% T137T (ACA→ACT)  elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273997,6730AT98.3% 925.1 / 1.8 281T137T (ACA→ACT) elfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base T (159/114);  total (165/116)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 290 total aligned reads displayed.

AGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCG  >  CP009273/997527‑997819
                                                                                                                                                  |                                                                                                                                                  
aGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggg                                                                                                                                                 >  1:613777/1‑150 (MQ=255)
aGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggg                                                                                                                                                 <  1:3711823/150‑1 (MQ=255)
  tGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggggg                                                                                                                                               <  2:622847/150‑1 (MQ=255)
  tGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggggg                                                                                                                                               >  1:234242/1‑150 (MQ=255)
    gCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggggggg                                                                                                                                             >  1:5037386/1‑150 (MQ=255)
      cGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGAt                                                                                                                                           <  2:3151055/150‑1 (MQ=255)
      cGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGAt                                                                                                                                           >  2:4182721/1‑150 (MQ=255)
          gggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATAccc                                                                                                                                       >  2:4857009/1‑150 (MQ=255)
           ggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCg                                                                                                                                      <  2:2304166/150‑1 (MQ=255)
            gCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGAAAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGa                                                                                                                                     >  1:688301/1‑150 (MQ=255)
                 cgccgcGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCa                                                                                                                                >  1:5103817/1‑150 (MQ=255)
                      cgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATAttt                                                                                                                           <  1:961419/150‑1 (MQ=255)
                      cgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATAttt                                                                                                                           <  2:4521007/150‑1 (MQ=255)
                         aTTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCg                                                                                                                        >  1:2697310/1‑150 (MQ=255)
                          ttCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGa                                                                                                                       <  1:3543215/150‑1 (MQ=255)
                              ccGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCt                                                                                                                   <  2:4438014/150‑1 (MQ=255)
                                gTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTgg                                                                                                                 <  2:3157285/150‑1 (MQ=255)
                                  tAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCg                                                                                                               >  1:1886155/1‑150 (MQ=255)
                                       tATTGAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCaa                                                                                                          <  2:4800841/150‑1 (MQ=255)
                                         tttAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACg                                                                                                        <  1:2930226/150‑1 (MQ=255)
                                           tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGtt                                                                                                      <  2:4433945/150‑1 (MQ=255)
                                           tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGtt                                                                                                      <  1:4662451/150‑1 (MQ=255)
                                           tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGtt                                                                                                      <  2:1884312/150‑1 (MQ=255)
                                              gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg                                                                                                   <  1:4072979/150‑1 (MQ=255)
                                                gACAACTGGTACAAAATTAATGACTATCTTTCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGGTCTCCTACTGATGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGAATATTTGCGACACTGGCGGACAACGTTTAGGt                                                                                                 >  1:5078559/1‑150 (MQ=255)
                                                gACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt                                                                                                 >  2:561835/1‑150 (MQ=255)
                                                   aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                                              >  1:1710726/1‑150 (MQ=255)
                                                   aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                                              <  1:2477345/150‑1 (MQ=255)
                                                   aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGATTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                                              >  1:4395376/1‑150 (MQ=255)
                                                    aCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGc                                                                                             >  2:3752872/1‑150 (MQ=255)
                                                     cTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCa                                                                                            <  2:2662514/150‑1 (MQ=255)
                                                        gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggg                                                                                         <  1:4221945/150‑1 (MQ=255)
                                                        gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggg                                                                                         <  1:4812877/150‑1 (MQ=255)
                                                        gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggg                                                                                         <  1:4857009/150‑1 (MQ=255)
                                                           cAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGc                                                                                      >  2:1164501/1‑150 (MQ=255)
                                                           cAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGGTTAGGTGGGCAGGGGGc                                                                                      >  2:3918098/1‑150 (MQ=255)
                                                             aaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCaa                                                                                    >  2:4011274/1‑150 (MQ=255)
                                                             aaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATATTCGACCCTCGCGGTCAACGATTCGGTGGGCAGGGGGCaa                                                                                    >  2:4752629/1‑150 (MQ=255)
                                                              aaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAg                                                                                   <  1:212194/150‑1 (MQ=255)
                                                               aTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGc                                                                                  >  2:1335062/1‑150 (MQ=255)
                                                                ttAATGACGATCTTGCCGCCAAAGTTTTATTGCAGGTTACAGGCAGTTCTCCTACTGCGGTTCCTTTCGACACCGTGGGCACTGGGGGGGAGACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCg                                                                                 <  2:1416831/150‑1 (MQ=255)
                                                                       cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAg                                                                          >  1:3590617/1‑150 (MQ=255)
                                                                       cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCCTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAg                                                                          <  2:3241750/150‑1 (MQ=255)
                                                                        tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGc                                                                         >  2:5056592/1‑150 (MQ=255)
                                                                        tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGc                                                                         >  2:106568/1‑150 (MQ=255)
                                                                        tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGc                                                                         <  2:2465360/150‑1 (MQ=255)
                                                                         aTCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCg                                                                        >  1:1506792/1‑150 (MQ=255)
                                                                           cTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                                      >  1:3639831/1‑150 (MQ=255)
                                                                           cTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGGGTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                                      >  2:4832974/1‑150 (MQ=255)
                                                                             tGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAg                                                                    >  2:2096640/1‑150 (MQ=255)
                                                                             tGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAg                                                                    <  1:389734/150‑1 (MQ=255)
                                                                             tGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGGTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAg                                                                    <  1:3240575/150‑1 (MQ=255)
                                                                               ccgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCt                                                                  <  2:2815500/150‑1 (MQ=255)
                                                                                cgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtt                                                                 >  2:3412232/1‑150 (MQ=255)
                                                                                cgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtt                                                                 >  1:4655/1‑150 (MQ=255)
                                                                                 gccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCttt                                                                >  1:3753784/1‑150 (MQ=255)
                                                                                  ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtttt                                                               <  2:1493152/150‑1 (MQ=255)
                                                                                  ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtttt                                                               <  1:852314/150‑1 (MQ=255)
                                                                                     aaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCTTATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTccc                                                            <  1:1157081/150‑1 (MQ=255)
                                                                                      aGTTTTATTGCAGGTTAAAGGCAGGTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                                           <  2:1964146/150‑1 (MQ=255)
                                                                                        ttttATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGa                                                         >  2:2228731/1‑150 (MQ=255)
                                                                                         tttATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaa                                                        >  2:1614268/1‑150 (MQ=255)
                                                                                          ttATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaaa                                                       >  1:3452120/1‑150 (MQ=255)
                                                                                           tATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaaaa                                                      >  1:2770250/1‑150 (MQ=255)
                                                                                           tATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaaaa                                                      >  1:3086583/1‑150 (MQ=255)
                                                                                            aTTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAat                                                     >  2:2605063/1‑150 (MQ=255)
                                                                                             ttGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAata                                                    <  2:2330177/150‑1 (MQ=255)
                                                                                               gCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATAtt                                                  >  1:4565626/1‑150 (MQ=255)
                                                                                                    ttAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                                             <  2:1652814/150‑1 (MQ=255)
                                                                                                    ttAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                                             >  2:3570828/1‑150 (MQ=255)
                                                                                                       aaGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgt                                          <  2:2509102/150‑1 (MQ=255)
                                                                                                        aGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgtt                                         >  2:4633237/1‑150 (MQ=255)
                                                                                                         ggCAGTTCTCCTACTGCGGTTCCTTTCGACACCGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttc                                        <  2:1780067/150‑1 (MQ=255)
                                                                                                          gCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcg                                       <  2:84750/150‑1 (MQ=255)
                                                                                                           cAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgt                                      <  1:2858968/150‑1 (MQ=255)
                                                                                                           cAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgt                                      <  2:2981169/150‑1 (MQ=255)
                                                                                                           aaGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGTTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgt                                      >  2:782211/2‑150 (MQ=255)
                                                                                                            aGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgtt                                     >  1:1104249/1‑150 (MQ=255)
                                                                                                             gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTg                                    <  2:1039342/150‑1 (MQ=255)
                                                                                                              ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTgg                                   >  2:2063348/1‑150 (MQ=255)
                                                                                                                ctcCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCt                                 >  1:2105406/1‑150 (MQ=255)
                                                                                                                 tcCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTc                                >  2:1309194/1‑150 (MQ=255)
                                                                                                                    tACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt                             <  2:3804189/150‑1 (MQ=255)
                                                                                                                    tACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt                             >  2:3976883/1‑150 (MQ=255)
                                                                                                                    tACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt                             >  1:3569230/1‑150 (MQ=255)
                                                                                                                    tACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt                             >  1:2419553/1‑150 (MQ=255)
                                                                                                                       tGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcgt                          >  2:500429/1‑150 (MQ=255)
                                                                                                                         cGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCa                        <  2:4262457/150‑1 (MQ=255)
                                                                                                                             tCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtcc                    >  2:4650850/1‑150 (MQ=255)
                                                                                                                               cTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCAGTTCGTTGGCTCGGTCGTCATtcctc                  >  2:305804/1‑150 (MQ=255)
                                                                                                                                   cGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAt              >  1:3006046/1‑150 (MQ=255)
                                                                                                                                   cGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAt              >  2:3651186/1‑150 (MQ=255)
                                                                                                                                      aaaCGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggc           <  1:48410/150‑1 (MQ=255)
                                                                                                                                          gTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggcgctg       >  2:4000596/1‑150 (MQ=255)
                                                                                                                                           tGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggcgctgg      >  1:3945123/1‑150 (MQ=255)
                                                                                                                                             ggCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggcgctggcg    >  1:1330252/1‑150 (MQ=255)
                                                                                                                                              gCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggcgctggcgc   >  1:2138364/1‑150 (MQ=255)
                                                                                                                                               cactGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGcgcg  <  2:5037386/150‑1 (MQ=255)
                                                                                                                                               cactGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGcgcg  >  1:197635/1‑150 (MQ=255)
                                                                                                                                               cactGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGcgcg  >  1:4498488/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCG  >  CP009273/997527‑997819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑