Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,038,316 G→T 100% R451S (CGT→AGT)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,038,3160GT97.2% 781.0 / 10.7 248R451S (CGT→AGT) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base T (137/104);  total (143/105)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAC  >  CP009273/1038169‑1038462
                                                                                                                                                   |                                                                                                                                                  
  atatTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGc                                                                                                                                                <  1:732455/150‑1 (MQ=255)
   tatTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCa                                                                                                                                               <  1:695527/150‑1 (MQ=255)
      ttttAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACa                                                                                                                                            >  1:3495062/1‑150 (MQ=255)
            tACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCAc                                                                                                                                      <  1:1503429/150‑1 (MQ=255)
            tACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCAc                                                                                                                                      >  1:1659376/1‑150 (MQ=255)
              cGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCAcgc                                                                                                                                    >  1:289150/1‑150 (MQ=255)
                 tGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGcc                                                                                                                                 >  1:463878/1‑150 (MQ=255)
                  ggCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCa                                                                                                                                >  2:1662591/1‑150 (MQ=255)
                       tgctgATTAGAAAATAAATTTTTCTTACTCAGACGGGAGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACAACACTACTCAACATCGCACGCGCCAGCACg                                                                                                                           >  2:1949224/1‑150 (MQ=255)
                       tgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACAGTGGGATAGTGGCATAAACGCTGATGCCGAGCTCTTCCAGTTGTTCCGGGGCTTCTAAACCACTACGCAACAACGCACGCGCCAGCACg                                                                                                                           >  2:872285/1‑150 (MQ=255)
                           gATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                       <  2:2350578/150‑1 (MQ=255)
                           gATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                       >  2:1071188/1‑150 (MQ=255)
                            aTTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAcc                                                                                                                      <  1:1370632/150‑1 (MQ=255)
                             ttAGAAAATAAATTTGTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAccc                                                                                                                     <  2:1121237/150‑1 (MQ=255)
                             ttAGAAAATAAATTTGTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAcca                                                                                                                     <  2:2949400/150‑2 (MQ=255)
                              tAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCca                                                                                                                    >  1:3933895/1‑150 (MQ=255)
                               aGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcac                                                                                                                   <  2:155376/150‑1 (MQ=255)
                                 aaaaTAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAg                                                                                                                 <  2:670382/150‑1 (MQ=255)
                                    ataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaaa                                                                                                              >  2:2188251/1‑150 (MQ=255)
                                      aaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATa                                                                                                            >  2:1038731/1‑150 (MQ=255)
                                         tttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAAc                                                                                                         <  2:376929/150‑1 (MQ=255)
                                          ttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACa                                                                                                        >  2:4715548/1‑150 (MQ=255)
                                          ttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACa                                                                                                        <  2:1190037/150‑1 (MQ=255)
                                           tttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAg                                                                                                       >  1:756647/1‑150 (MQ=255)
                                              cTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGcc                                                                                                    >  2:4590082/1‑150 (MQ=255)
                                              cTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGcc                                                                                                    >  1:1114722/1‑150 (MQ=255)
                                               ttACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGccc                                                                                                   >  2:2747792/1‑149 (MQ=255)
                                               ttACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCa                                                                                                   >  2:51366/1‑150 (MQ=255)
                                               ttACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCa                                                                                                   >  2:3106918/1‑150 (MQ=255)
                                                 aCGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAg                                                                                                 >  1:1555773/1‑150 (MQ=255)
                                                  cGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGa                                                                                                >  1:4466822/1‑150 (MQ=255)
                                                   gCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGaa                                                                                               >  1:5012627/1‑150 (MQ=255)
                                                     aGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATa                                                                                             >  2:3572004/1‑150 (MQ=255)
                                                      gACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGTCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaa                                                                                            <  2:4895905/150‑1 (MQ=255)
                                                       aCGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaaa                                                                                           <  1:1177360/150‑1 (MQ=255)
                                                         gggTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAAc                                                                                         >  1:3450824/1‑150 (MQ=255)
                                                            tGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCaa                                                                                      <  2:38771/150‑1 (MQ=255)
                                                            tGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCaa                                                                                      <  2:4429404/150‑1 (MQ=255)
                                                             gCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAg                                                                                     >  1:4669460/1‑150 (MQ=255)
                                                               gTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGca                                                                                   <  1:4085863/150‑1 (MQ=255)
                                                                 ttATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcacc                                                                                 >  2:1277228/1‑150 (MQ=255)
                                                                   aTCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcaccac                                                                               >  1:1997999/1‑150 (MQ=255)
                                                                    tCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACg                                                                              <  2:2787152/150‑1 (MQ=255)
                                                                     ccAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGt                                                                             >  1:2686400/1‑150 (MQ=255)
                                                                     ccAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGt                                                                             >  2:3509297/1‑150 (MQ=255)
                                                                      cAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGtt                                                                            <  1:2138104/150‑1 (MQ=255)
                                                                       aGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTc                                                                           <  2:4968197/150‑1 (MQ=255)
                                                                           aCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACg                                                                       <  1:204953/150‑1 (MQ=255)
                                                                           aCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACg                                                                       <  1:3985659/150‑1 (MQ=255)
                                                                             tCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCt                                                                     <  1:4898462/150‑1 (MQ=255)
                                                                             tCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCt                                                                     <  1:4898332/150‑1 (MQ=255)
                                                                             tCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCt                                                                     >  1:4863978/1‑150 (MQ=255)
                                                                                gACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtttt                                                                  >  1:1254373/1‑150 (MQ=255)
                                                                                    ttGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCg                                                              >  1:136830/1‑150 (MQ=255)
                                                                                     tGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCgg                                                             <  1:2188192/150‑1 (MQ=255)
                                                                                     tGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCgg                                                             <  1:4061233/150‑1 (MQ=255)
                                                                                       ggATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGtt                                                           >  2:1949112/1‑150 (MQ=255)
                                                                                        gATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGttt                                                          >  2:1419989/1‑150 (MQ=255)
                                                                                        gATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGttt                                                          >  2:4404385/1‑150 (MQ=255)
                                                                                         aTAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTc                                                         >  1:555685/1‑150 (MQ=255)
                                                                                          tAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCa                                                        >  2:1817052/1‑150 (MQ=255)
                                                                                           aGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCAc                                                       <  1:314736/150‑1 (MQ=255)
                                                                                           aGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCAc                                                       <  2:4100513/150‑1 (MQ=255)
                                                                                            gTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACt                                                      >  1:2806272/1‑150 (MQ=255)
                                                                                            gTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACt                                                      >  2:390924/1‑150 (MQ=255)
                                                                                            gTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACt                                                      >  2:3301403/1‑150 (MQ=255)
                                                                                              ggCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTACAACGCTTTTTTCGGTTTCACTgg                                                    >  2:1481090/1‑150 (MQ=255)
                                                                                                   aaaCGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCg                                               >  1:3764604/1‑150 (MQ=255)
                                                                                                    aaCGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCgg                                              <  2:362799/150‑1 (MQ=255)
                                                                                                     aCGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGc                                             <  2:4064989/150‑1 (MQ=255)
                                                                                                      cGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCt                                            <  2:255862/150‑1 (MQ=255)
                                                                                                      cGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCt                                            >  1:4217564/1‑150 (MQ=255)
                                                                                                       gCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGGTCAACGCTTTTTTCGGTGTCACTGGCTGCGGCTg                                           >  2:5158782/1‑150 (MQ=255)
                                                                                                            tGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGa                                      >  1:4977715/1‑150 (MQ=255)
                                                                                                              ccGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACt                                    <  1:4448977/150‑1 (MQ=255)
                                                                                                              ccGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACt                                    >  2:826533/1‑150 (MQ=255)
                                                                                                               cGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTg                                   <  2:246828/150‑1 (MQ=255)
                                                                                                                gTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGc                                  >  2:3903574/1‑150 (MQ=255)
                                                                                                                 tGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGcc                                 <  1:4867059/150‑1 (MQ=255)
                                                                                                                 tGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGcc                                 <  2:4766544/150‑1 (MQ=255)
                                                                                                                   ctctTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCgg                               >  2:2761261/1‑150 (MQ=255)
                                                                                                                     ctTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGt                             >  1:2560320/1‑150 (MQ=255)
                                                                                                                       tCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCg                           >  2:1107224/1‑150 (MQ=255)
                                                                                                                        ccAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGa                          >  2:609501/1‑150 (MQ=255)
                                                                                                                         cAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGAt                         >  2:3266932/1‑150 (MQ=255)
                                                                                                                         cAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGAt                         <  1:1098691/150‑1 (MQ=255)
                                                                                                                          aGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATa                        >  2:350011/1‑150 (MQ=255)
                                                                                                                            ttgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAt                      <  2:2132602/150‑1 (MQ=255)
                                                                                                                             tgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATc                     >  1:878344/1‑150 (MQ=255)
                                                                                                                             tgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATc                     >  2:2828822/1‑150 (MQ=255)
                                                                                                                                tCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGc                  >  1:4408634/1‑150 (MQ=255)
                                                                                                                                 ccGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCa                 <  1:631896/150‑1 (MQ=255)
                                                                                                                                 ccGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCa                 <  1:3334552/150‑1 (MQ=255)
                                                                                                                                  cGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCAc                >  1:1028106/1‑150 (MQ=255)
                                                                                                                                   ggggCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACg               >  2:706395/1‑150 (MQ=255)
                                                                                                                                     ggCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGtt             >  1:1777485/1‑150 (MQ=255)
                                                                                                                                         tCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCa         >  1:831251/1‑150 (MQ=255)
                                                                                                                                            acacCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATg      <  1:3587257/150‑1 (MQ=255)
                                                                                                                                              accacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGc    <  2:2427399/150‑1 (MQ=255)
                                                                                                                                                cacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAc  >  2:1300646/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAC  >  CP009273/1038169‑1038462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑