Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,146,692 A→T 100% T189T (ACA→ACT)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,146,6920AT98.1% 1060.5 / 5.4 319T189T (ACA→ACT) fabG3‑oxoacyl‑[acyl‑carrier‑protein] reductase
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base T (167/146);  total (171/148)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 319 total aligned reads displayed.

TGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAA  >  CP009273/1146545‑1146838
                                                                                                                                                   |                                                                                                                                                  
 ggtACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGt                                                                                                                                                 <  2:3262894/150‑1 (MQ=255)
  gtACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTg                                                                                                                                                >  1:3007582/1‑150 (MQ=255)
   tACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgc                                                                                                                                               <  1:3004704/150‑1 (MQ=255)
        ggggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGa                                                                                                                                          <  2:4691098/149‑1 (MQ=255)
          ggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGc                                                                                                                                        >  1:2432740/1‑150 (MQ=255)
           gAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGCAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCg                                                                                                                                       <  2:245681/150‑1 (MQ=255)
            aaaTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                      <  2:3121693/150‑1 (MQ=255)
              aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATg                                                                                                                                    >  2:4654399/1‑150 (MQ=255)
                   ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAg                                                                                                                               >  2:3463420/1‑150 (MQ=255)
                   ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAg                                                                                                                               <  1:4949415/150‑1 (MQ=255)
                    gTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGc                                                                                                                              <  2:4100405/150‑1 (MQ=255)
                        ggCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGc                                                                                                                          <  1:1234788/150‑1 (MQ=255)
                         gCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCg                                                                                                                         >  1:320625/1‑150 (MQ=255)
                          ccAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCgg                                                                                                                        <  1:2129495/150‑1 (MQ=255)
                          ccAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCgg                                                                                                                        >  2:783311/1‑150 (MQ=255)
                          ccAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCgg                                                                                                                        >  2:3289741/1‑150 (MQ=255)
                             aCTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTa                                                                                                                     >  1:3292280/1‑150 (MQ=255)
                             aCTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTa                                                                                                                     <  2:503693/150‑1 (MQ=255)
                               tACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGtctc                                                                                                                   >  2:3368627/1‑150 (MQ=255)
                               tACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATc                                                                                                                   >  2:492574/1‑150 (MQ=255)
                               tACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATc                                                                                                                   >  1:3788200/1‑150 (MQ=255)
                               tACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATc                                                                                                                   >  2:259964/1‑150 (MQ=255)
                                   cTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                               >  2:43114/1‑150 (MQ=255)
                                    tGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGgc                                                                                                              <  2:3926832/150‑1 (MQ=255)
                                     gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCAGgcg                                                                                                             >  1:1628234/1‑150 (MQ=255)
                                      cggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTCTCCTGgcgc                                                                                                            >  2:4780671/1‑150 (MQ=255)
                                      cggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGgcgc                                                                                                            >  2:4514389/1‑150 (MQ=255)
                                      cggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGgcgc                                                                                                            >  2:4438582/1‑150 (MQ=255)
                                       ggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCa                                                                                                           >  1:3118093/1‑150 (MQ=255)
                                          gAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGt                                                                                                        >  1:4468098/1‑150 (MQ=255)
                                           aaaGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGtt                                                                                                       >  1:1269784/1‑150 (MQ=255)
                                            aaGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                      >  1:2344325/1‑150 (MQ=255)
                                              gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCt                                                                                                    <  2:855396/150‑1 (MQ=255)
                                                  gCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCgg                                                                                                <  1:383863/150‑1 (MQ=255)
                                                   cTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCggg                                                                                               <  2:1720618/150‑1 (MQ=255)
                                                    ttGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGt                                                                                              <  2:3509455/150‑1 (MQ=255)
                                                     tGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTc                                                                                             <  1:1315122/150‑1 (MQ=255)
                                                        tCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGcc                                                                                          >  2:3946800/1‑150 (MQ=255)
                                                         cGGATTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCt                                                                                         <  1:693375/150‑1 (MQ=255)
                                                          ggCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTc                                                                                        <  1:60846/150‑1 (MQ=255)
                                                          ggCGTCAGTAACTCACTGGCGCGCGAAGGTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTc                                                                                        <  2:2614954/150‑1 (MQ=255)
                                                           gCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcg                                                                                       >  1:2819521/1‑150 (MQ=255)
                                                               cAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcggcgg                                                                                   >  2:1350917/1‑150 (MQ=255)
                                                               cAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcggcgg                                                                                   >  2:4519739/1‑150 (MQ=255)
                                                                 gTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGgcggcg                                                                                 >  1:945326/1‑150 (MQ=255)
                                                                  tAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGgcgc                                                                                <  1:1855648/150‑1 (MQ=255)
                                                                   aaaTCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGca                                                                               >  2:3137479/1‑150 (MQ=255)
                                                                    aaTCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGcac                                                                              >  1:3354814/1‑150 (MQ=255)
                                                                        aCTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGa                                                                          >  1:1716414/1‑150 (MQ=255)
                                                                           ggCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAAt                                                                       >  2:1654581/1‑150 (MQ=255)
                                                                             cgcgcgAAGGTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                     <  1:2949314/150‑1 (MQ=255)
                                                                              gcgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGc                                                                    <  2:2384617/150‑1 (MQ=255)
                                                                                       tGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGt                                                           <  2:1604434/150‑1 (MQ=255)
                                                                                            cACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                      >  1:4838898/1‑150 (MQ=255)
                                                                                              cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                    <  1:1481738/150‑1 (MQ=255)
                                                                                               gcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTcc                                                   >  1:4542445/1‑150 (MQ=255)
                                                                                               gcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTcc                                                   >  2:2111976/1‑150 (MQ=255)
                                                                                                cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCt                                                  <  1:3933913/150‑1 (MQ=255)
                                                                                                 ggTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTg                                                 <  1:201839/150‑1 (MQ=255)
                                                                                                 ggTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTg                                                 <  2:4206116/150‑1 (MQ=255)
                                                                                                   tATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGc                                               <  1:4889690/150‑1 (MQ=255)
                                                                                                   tATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGc                                               >  1:3568919/1‑150 (MQ=255)
                                                                                                       aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                           <  2:4757897/150‑1 (MQ=255)
                                                                                                        cTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcg                                          >  2:4565399/1‑150 (MQ=255)
                                                                                                          gttaaCGTTGTTTCTCAGTTCTTTATTGAATCGGACATGACTCGTGAGCTGAGCGATGACAAGCGTGCGTGTATCCTGGGGCAGGTTCATGCGTGTCGCCTCGGCGGCTAACAGGAAATCGCCAACGATGTTGCATTCCTGGCATCcgac                                        <  2:5112075/147‑1 (MQ=255)
                                                                                                           tAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGAGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacg                                       >  1:463360/1‑150 (MQ=255)
                                                                                                            aaaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                      >  2:2148619/1‑150 (MQ=255)
                                                                                                            aaaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                      >  2:3725831/1‑150 (MQ=255)
                                                                                                            aaaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                      >  1:4743016/1‑150 (MQ=255)
                                                                                                             aaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGaa                                     >  2:754485/1‑150 (MQ=255)
                                                                                                              aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAag                                    <  2:651890/150‑1 (MQ=255)
                                                                                                                   gttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCt                               <  1:4088411/150‑1 (MQ=255)
                                                                                                                    ttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCtt                              <  2:901612/150‑1 (MQ=255)
                                                                                                                     tgCTCCGGGCTTTATTGAAACGGACCTGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTa                             <  1:438953/150‑1 (MQ=255)
                                                                                                                     tgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTa                             <  2:607502/150‑1 (MQ=255)
                                                                                                                       cTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACa                           >  1:4310086/1‑150 (MQ=255)
                                                                                                                        tCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAt                          >  1:3795429/1‑150 (MQ=255)
                                                                                                                          cGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCa                        >  2:2972342/1‑150 (MQ=255)
                                                                                                                          cGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCa                        >  1:3434688/1‑150 (MQ=255)
                                                                                                                          cGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCa                        >  2:1478497/1‑150 (MQ=255)
                                                                                                                             gCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACgg                     <  2:108949/150‑1 (MQ=255)
                                                                                                                                ttATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTg                  <  2:1249315/150‑1 (MQ=255)
                                                                                                                                ttATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTg                  <  1:4701535/150‑1 (MQ=255)
                                                                                                                                 tATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGa                 <  2:3051821/150‑1 (MQ=255)
                                                                                                                                 tATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGa                 <  1:4142431/150‑1 (MQ=255)
                                                                                                                                  aTTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGaa                >  2:3809634/1‑150 (MQ=255)
                                                                                                                                  aTTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGaa                <  2:3200502/150‑1 (MQ=255)
                                                                                                                                    tGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAAc              <  1:516116/150‑1 (MQ=255)
                                                                                                                                    tGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAAc              <  2:286368/150‑1 (MQ=255)
                                                                                                                                     gAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACt             >  2:1527178/1‑150 (MQ=255)
                                                                                                                                     gAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACt             <  1:2413050/150‑1 (MQ=255)
                                                                                                                                      aaaCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt            <  1:2702302/150‑1 (MQ=255)
                                                                                                                                      aaaCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt            >  1:1944486/1‑150 (MQ=255)
                                                                                                                                      aaaCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt            <  1:1692212/150‑1 (MQ=255)
                                                                                                                                        aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTg          <  1:2495403/150‑1 (MQ=255)
                                                                                                                                         cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACCTTGc         >  1:1136748/1‑150 (MQ=255)
                                                                                                                                           gACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAt       <  1:783320/150‑1 (MQ=255)
                                                                                                                                            aCATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtg      >  2:1140928/1‑150 (MQ=255)
                                                                                                                                               tGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGa   <  1:259964/150‑1 (MQ=255)
                                                                                                                                                gACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGaa  <  2:815985/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAA  >  CP009273/1146545‑1146838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑