Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,156,310 G→T 100% S233* (TCA→TAA)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,156,3100GT99.0% 1017.9 / ‑1.0 313S233* (TCA→TAA) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (158/151);  total (161/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 313 total aligned reads displayed.

TTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCG  >  CP009273/1156163‑1156456
                                                                                                                                                   |                                                                                                                                                  
  ccAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTc                                                                                                                                                <  1:3966160/150‑1 (MQ=255)
   cAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCg                                                                                                                                               >  2:1104955/1‑150 (MQ=255)
    aGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGt                                                                                                                                              >  1:1955557/1‑150 (MQ=255)
     gcgcGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGtt                                                                                                                                             <  2:617484/150‑1 (MQ=255)
     gcgcGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGtt                                                                                                                                             <  2:202471/150‑1 (MQ=255)
      cgcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                            >  1:2883793/1‑150 (MQ=255)
       gcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTAt                                                                                                                                           <  2:1017267/150‑1 (MQ=255)
       gcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTAt                                                                                                                                           <  2:3546217/150‑1 (MQ=255)
         ggTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTc                                                                                                                                         >  2:1386415/1‑150 (MQ=255)
           tAAACCGCCCCAGGTAGGGCTATTCACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTg                                                                                                                                       <  2:2663154/150‑1 (MQ=255)
           tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGTTATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTg                                                                                                                                       <  1:159135/150‑1 (MQ=255)
           tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTg                                                                                                                                       >  1:2817692/1‑150 (MQ=255)
            aaaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTgg                                                                                                                                      >  1:2702804/1‑150 (MQ=255)
            aaaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTgg                                                                                                                                      <  2:548214/150‑1 (MQ=255)
             aaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGt                                                                                                                                     >  2:579132/1‑150 (MQ=255)
             aaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGt                                                                                                                                     >  2:2716596/1‑150 (MQ=255)
               ccGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAg                                                                                                                                   >  2:345784/1‑150 (MQ=255)
                 gCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgcc                                                                                                                                 >  2:4970769/1‑150 (MQ=255)
                  ccccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                                >  2:3831632/1‑150 (MQ=255)
                    ccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccgcc                                                                                                                              <  2:1321654/150‑1 (MQ=255)
                     taGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGccgccg                                                                                                                             >  2:1968783/2‑150 (MQ=255)
                      aGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGa                                                                                                                            >  1:4932839/1‑150 (MQ=255)
                        gTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACa                                                                                                                          >  1:1913852/1‑150 (MQ=255)
                             gCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcg                                                                                                                     >  2:2274940/1‑150 (MQ=255)
                               tATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcg                                                                                                                   <  1:4467050/150‑1 (MQ=255)
                               tATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcg                                                                                                                   >  1:1766551/1‑150 (MQ=255)
                                    aCATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCgg                                                                                                              >  1:3680599/1‑150 (MQ=255)
                                     cATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGa                                                                                                             <  1:4038221/150‑1 (MQ=255)
                                        cAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAttt                                                                                                          >  2:124174/1‑150 (MQ=255)
                                           tGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAc                                                                                                       >  1:1680242/1‑150 (MQ=255)
                                           tGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAc                                                                                                       <  1:1558174/150‑1 (MQ=255)
                                            gcgcTGAGAGTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAcc                                                                                                      <  2:4826779/150‑1 (MQ=255)
                                                tGATATTCGTATCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTGCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                  <  1:2330453/150‑1 (MQ=255)
                                                tGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                  <  1:1005055/150‑1 (MQ=255)
                                                 gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCtt                                                                                                 <  1:4329124/150‑1 (MQ=255)
                                                 gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCtt                                                                                                 <  1:2417041/150‑1 (MQ=255)
                                                   tatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                               >  2:1603347/1‑150 (MQ=255)
                                                   tatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                               <  1:2668116/150‑1 (MQ=255)
                                                   tatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                               <  1:4554781/150‑1 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                              >  2:918716/1‑150 (MQ=255)
                                                    atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCAATTAGTCGTTATTCTGGTAGCCGCCGACAATTCTCGCGCGGATTTTACCGTCTTCgg                                                                                              >  2:896435/1‑150 (MQ=255)
                                                     ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGt                                                                                             <  2:2859539/150‑1 (MQ=255)
                                                      tCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTg                                                                                            >  1:1507359/1‑150 (MQ=255)
                                                      tCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTg                                                                                            <  1:903270/150‑1 (MQ=255)
                                                       cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGa                                                                                           >  2:2411963/1‑150 (MQ=255)
                                                          aaCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                        >  1:776032/1‑150 (MQ=255)
                                                            ccGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTggg                                                                                      >  1:3917737/1‑150 (MQ=255)
                                                                cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCttt                                                                                  <  2:1679901/150‑1 (MQ=255)
                                                                 tGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCATCAATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTg                                                                                 >  2:4962876/1‑150 (MQ=255)
                                                                  gAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGt                                                                                >  2:4776656/1‑150 (MQ=255)
                                                                  caaaGCGTCGTAAGATCGCCTAAATCAGCATCGACCATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGt                                                                                >  2:3515780/2‑150 (MQ=255)
                                                                     aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTaaa                                                                             <  1:5050542/150‑1 (MQ=255)
                                                                     aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTaaa                                                                             <  1:4551359/150‑1 (MQ=255)
                                                                       cgtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATc                                                                           >  2:4630137/1‑150 (MQ=255)
                                                                        gtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATcc                                                                          <  1:1705872/150‑1 (MQ=255)
                                                                        gtcgtAAGATAGCCTAAATCAGCATCGAAAATGCCCGAGAAGAATGTCTTTTCACTGTTGTAGCGGTCCAGAAATTAGTCGTTATTCTGTTAGCCTACTACAATTAGCGCGCGGATTTTACCGTCTTCTGTGAGTGGGCTTTGTAAATca                                                                          >  2:4940593/1‑149 (MQ=255)
                                                                              aGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcaca                                                                    >  2:3787634/1‑150 (MQ=255)
                                                                              aGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcaca                                                                    >  1:3302591/1‑150 (MQ=255)
                                                                               gATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                   <  1:637084/150‑1 (MQ=255)
                                                                                 tCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAc                                                                 >  1:521014/1‑150 (MQ=255)
                                                                                   cccTAAATCAGAAGAGACACTGCCCGAGAAGAAGGTCTTTACACAGTTGTAGCGCTCCAGCCAAAAGTCGTTATTCTGGTAGCCGACTAAACTTCGCGCGCGGCTTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAACg                                                               >  2:5119521/2‑150 (MQ=255)
                                                                                     cTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGtt                                                             <  2:2082228/150‑1 (MQ=255)
                                                                                        aaTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCtt                                                          <  1:4831764/150‑1 (MQ=255)
                                                                                                  gACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCt                                                >  1:559560/1‑150 (MQ=255)
                                                                                                   aCAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTa                                               <  1:3228502/150‑1 (MQ=255)
                                                                                                     aaTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTAcc                                             >  2:252957/1‑150 (MQ=255)
                                                                                                      aTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCg                                            >  2:3296522/1‑150 (MQ=255)
                                                                                                          ccGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAtt                                        >  2:3878565/1‑150 (MQ=255)
                                                                                                           cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTc                                       >  1:4888280/1‑150 (MQ=255)
                                                                                                            gagaAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTcc                                      <  1:706695/150‑1 (MQ=255)
                                                                                                            gagaAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTcc                                      <  1:1362771/150‑1 (MQ=255)
                                                                                                             tgaagaaGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccg                                     >  1:4206774/2‑150 (MQ=255)
                                                                                                              gaagaaGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgc                                    >  1:3007840/1‑150 (MQ=255)
                                                                                                               aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                   <  1:2902745/150‑1 (MQ=255)
                                                                                                               aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                   <  1:5089454/150‑1 (MQ=255)
                                                                                                                agaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgccg                                  >  2:67056/1‑150 (MQ=255)
                                                                                                                 gaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCga                                 <  2:1766551/150‑1 (MQ=255)
                                                                                                                 gaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCga                                 <  1:2212507/150‑1 (MQ=255)
                                                                                                                 gaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCga                                 >  1:2749100/1‑150 (MQ=255)
                                                                                                                  aagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgag                                >  1:2176938/1‑150 (MQ=255)
                                                                                                                    ggTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGAc                              >  2:4390456/1‑150 (MQ=255)
                                                                                                                     gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACa                             <  1:513279/150‑1 (MQ=255)
                                                                                                                      tCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACAt                            >  2:4087007/1‑150 (MQ=255)
                                                                                                                          ttCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGcc                        <  2:4453777/150‑1 (MQ=255)
                                                                                                                           tCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCt                       >  2:1993955/1‑150 (MQ=255)
                                                                                                                              cTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACAGTTCTTTGTTACAGCTACCGTCTTCCGCCGAGACATCGCCTTTa                    >  2:2537077/1‑150 (MQ=255)
                                                                                                                               tgttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaa                   >  2:3134099/1‑150 (MQ=255)
                                                                                                                                gttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaaa                  <  1:1107312/150‑1 (MQ=255)
                                                                                                                                gttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaaa                  >  1:513912/1‑150 (MQ=255)
                                                                                                                                 ttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAAt                 <  1:3787634/150‑1 (MQ=255)
                                                                                                                                   gtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTc               >  2:3980170/1‑150 (MQ=255)
                                                                                                                                   gtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTc               >  1:2608775/1‑150 (MQ=255)
                                                                                                                                   gtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTc               <  2:2379153/150‑1 (MQ=255)
                                                                                                                                      gCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACg            <  2:280967/150‑1 (MQ=255)
                                                                                                                                      gCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACg            <  2:2247872/150‑1 (MQ=255)
                                                                                                                                        ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc          >  1:2256246/1‑150 (MQ=255)
                                                                                                                                        ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc          <  2:1812424/150‑1 (MQ=255)
                                                                                                                                         gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt         <  2:3176099/150‑1 (MQ=255)
                                                                                                                                                cATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgcg  <  2:2919254/150‑1 (MQ=255)
                                                                                                                                                cATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgcg  >  2:2769658/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCG  >  CP009273/1156163‑1156456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑