Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 102,736 C→A 100% I315I (ATC→ATA)  ftsZ → GTP‑binding tubulin‑like cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273102,7360CA97.3% 827.0 / 11.1 268I315I (ATC→ATA) ftsZGTP‑binding tubulin‑like cell division protein
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base A (132/127);  total (138/130)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

GGGCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCA  >  CP009273/102589‑102882
                                                                                                                                                   |                                                                                                                                                  
gggCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCgg                                                                                                                                                  >  1:164148/1‑150 (MQ=255)
gggCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAgg                                                                                                                                                  >  1:3462790/1‑150 (MQ=255)
 ggCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGc                                                                                                                                                 <  2:1292583/150‑1 (MQ=255)
  gCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCa                                                                                                                                                >  1:4497105/1‑150 (MQ=255)
  gCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCa                                                                                                                                                >  2:1686259/1‑150 (MQ=255)
  gCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCa                                                                                                                                                >  1:2863250/1‑150 (MQ=255)
   cTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCAt                                                                                                                                               >  1:1794037/1‑150 (MQ=255)
    ttCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATg                                                                                                                                              <  2:1635704/150‑1 (MQ=255)
       gACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGAc                                                                                                                                           >  2:3384254/1‑150 (MQ=255)
        aCCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACa                                                                                                                                          >  2:3422368/1‑150 (MQ=255)
         ccTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACac                                                                                                                                         >  1:2614551/1‑149 (MQ=255)
         ccTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaa                                                                                                                                         <  1:322932/150‑1 (MQ=255)
             cGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGt                                                                                                                                     <  1:2247487/150‑1 (MQ=255)
               tCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTcc                                                                                                                                   <  2:4989879/150‑1 (MQ=255)
                cTGGATGATTTCGATCCGGTAGGTACCACCATGCGTGCATTTGCTTCCGACAACGCGACTGTGTTTATAGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACNGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCt                                                                                                                                  <  1:2677282/150‑1 (MQ=255)
                cTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCt                                                                                                                                  >  1:3210360/1‑150 (MQ=255)
                 tGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTg                                                                                                                                 <  2:4506793/150‑1 (MQ=255)
                  ggATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGa                                                                                                                                <  2:4323801/150‑1 (MQ=255)
                   gATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaa                                                                                                                               <  1:2164240/150‑1 (MQ=255)
                      gAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATc                                                                                                                            <  1:2239829/150‑1 (MQ=255)
                      gAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATc                                                                                                                            <  1:685295/150‑1 (MQ=255)
                         ttCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCAct                                                                                                                         <  2:1073297/150‑1 (MQ=255)
                          tCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGACATCActc                                                                                                                        >  1:217769/1‑150 (MQ=255)
                          tCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCActc                                                                                                                        <  1:2736705/150‑1 (MQ=255)
                           cGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCActct                                                                                                                       <  2:3748052/150‑1 (MQ=255)
                           cGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACGGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCTCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCActct                                                                                                                       >  2:3108699/1‑150 (MQ=255)
                            gAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTg                                                                                                                      >  1:125578/1‑150 (MQ=255)
                            gAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTg                                                                                                                      <  2:4195227/150‑1 (MQ=255)
                             aaaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTgg                                                                                                                     >  2:4583010/1‑150 (MQ=255)
                                 ggtaggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                 >  2:2974192/1‑150 (MQ=255)
                                 ggtaggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                 <  2:3817212/150‑1 (MQ=255)
                                   taggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCa                                                                                                               >  2:1451867/1‑150 (MQ=255)
                                   taggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAAGGACGAGCTGCGCGTAACCGTTGTTGCGACAGTTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCa                                                                                                               <  1:1736490/150‑1 (MQ=255)
                                    aggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCaa                                                                                                              <  1:2067985/150‑1 (MQ=255)
                                     ggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAAt                                                                                                             <  1:957529/150‑1 (MQ=255)
                                           acCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAg                                                                                                       <  1:91590/150‑1 (MQ=255)
                                             cATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACGGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGt                                                                                                     >  1:823859/1‑150 (MQ=255)
                                              aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                    >  2:3645361/1‑150 (MQ=255)
                                               tCCGTGCATTTTCTTCCGACAACGCGACAGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGAGCGAAACCGTAGTTGACACAGGTATAGGCATGGACAAACGTCCTTAAATCACTCTGTTTACAAATAAGCAGttta                                                                                                   >  2:2964551/1‑149 (MQ=255)
                                                ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTca                                                                                                  >  2:116756/1‑150 (MQ=255)
                                                    gCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagcag                                                                                              <  2:57634/150‑1 (MQ=255)
                                                      aTTTGCGTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGcc                                                                                            <  2:4377128/150‑1 (MQ=255)
                                                       tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCa                                                                                           <  2:2008382/150‑1 (MQ=255)
                                                       tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCa                                                                                           >  1:3247137/1‑150 (MQ=255)
                                                         tGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGt                                                                                         >  1:4128662/1‑150 (MQ=255)
                                                         tGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGt                                                                                         >  2:279751/1‑150 (MQ=255)
                                                            ttCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                      >  2:668257/1‑150 (MQ=255)
                                                            ttCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGAt                                                                                      >  1:4432154/1‑150 (MQ=255)
                                                             tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                     >  2:2511184/1‑150 (MQ=255)
                                                              ccGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATgg                                                                                    <  2:1851644/150‑1 (MQ=255)
                                                               cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                                   >  1:694436/1‑150 (MQ=255)
                                                                 aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATc                                                                                 <  1:4669039/150‑1 (MQ=255)
                                                                   aaCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGc                                                                               >  2:4936442/1‑150 (MQ=255)
                                                                   aaCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGc                                                                               >  2:1200877/1‑150 (MQ=255)
                                                                    aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCt                                                                              <  1:597494/150‑1 (MQ=255)
                                                                    aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCt                                                                              <  1:4548875/150‑1 (MQ=255)
                                                                    aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCt                                                                              <  1:3744705/150‑1 (MQ=255)
                                                                     cgcgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTa                                                                             >  2:4757883/1‑150 (MQ=255)
                                                                     cgcgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTa                                                                             >  1:4296661/1‑150 (MQ=255)
                                                                       cgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGCCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                           <  1:1627409/150‑1 (MQ=255)
                                                                         aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcag                                                                         <  2:3898796/150‑1 (MQ=255)
                                                                          cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagc                                                                        >  2:2665009/1‑150 (MQ=255)
                                                                                ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATg                                                                  >  2:1886762/1‑150 (MQ=255)
                                                                                 tATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATgg                                                                 >  2:4130132/1‑150 (MQ=255)
                                                                                  aTCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                <  1:4637194/150‑1 (MQ=255)
                                                                                     ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATg                                                             <  2:4099541/150‑1 (MQ=255)
                                                                                      ttACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATgg                                                            >  2:2452549/2‑150 (MQ=255)
                                                                                        aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCt                                                          <  1:1904454/150‑1 (MQ=255)
                                                                                        aCTTCTCATGACACTGAAATGAAAGAAGAGATGAGAGTAACCGTAGTAGCGACCGGAATAGGCATGTACAAACGACCTGAAAAAACTCTGTATACAAATAAGCAGGTTCAGCAGCCAGAGATGGATCGATACCAGCATCATGGGAAggat                                                          >  2:221857/1‑148 (MQ=255)
                                                                                         cTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAGCAATAAGCAGGTTCAGCAGCCAGTGTTGGATCGCTACCAGCAGCATGGGATGGCTc                                                         <  1:4136725/150‑1 (MQ=255)
                                                                                         cTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTc                                                         >  2:2652016/1‑150 (MQ=255)
                                                                                           tctcTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCg                                                       >  1:3321026/1‑150 (MQ=255)
                                                                                             tctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCt                                                     <  2:949915/150‑1 (MQ=255)
                                                                                               ttGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGa                                                   <  1:2523041/150‑1 (MQ=255)
                                                                                               ttGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGa                                                   >  2:3833112/1‑150 (MQ=255)
                                                                                                tGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAc                                                  >  2:3408686/1‑150 (MQ=255)
                                                                                                 gACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGGGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAcc                                                 >  1:5020306/1‑150 (MQ=255)
                                                                                                 gACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAcc                                                 <  1:1023368/150‑1 (MQ=255)
                                                                                                  aCCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAccc                                                <  2:371598/150‑1 (MQ=255)
                                                                                                    ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAg                                              <  1:671570/150‑1 (MQ=255)
                                                                                                    ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAg                                              <  2:4036364/150‑1 (MQ=255)
                                                                                                     cGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAgg                                             >  1:983601/1‑150 (MQ=255)
                                                                                                      ggATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGa                                            <  2:511523/150‑1 (MQ=255)
                                                                                                         tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCa                                         <  1:1136415/150‑1 (MQ=255)
                                                                                                          atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAg                                        >  2:848644/1‑150 (MQ=255)
                                                                                                          atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAg                                        <  1:3100765/150‑1 (MQ=255)
                                                                                                               tgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGcc                                   >  1:542889/1‑150 (MQ=255)
                                                                                                               tgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGcc                                   <  1:2661438/150‑1 (MQ=255)
                                                                                                                gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCg                                  <  2:3312169/150‑1 (MQ=255)
                                                                                                                   gAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGtt                               <  2:1642708/150‑1 (MQ=255)
                                                                                                                   gAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGtt                               <  2:3791142/150‑1 (MQ=255)
                                                                                                                    aGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTg                              >  1:3663401/1‑150 (MQ=255)
                                                                                                                       tGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTa                           >  1:1963933/1‑150 (MQ=255)
                                                                                                                                  gttgttGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATACGCAGGTTCTGCAGCCAGGGATGGATCGCTCCCAGCAGCATGGGATGGCTCCGCTGCCCCAGGCGCGGCAGCCGGGTGCTCACGTCggggct                >  2:3136676/1‑145 (MQ=255)
                                                                                                                                      ttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACa            >  1:2997493/1‑150 (MQ=255)
                                                                                                                                         cGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg         <  1:3533547/150‑1 (MQ=255)
                                                                                                                                         cGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg         <  2:3247137/150‑1 (MQ=255)
                                                                                                                                         cGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg         >  1:4792108/1‑150 (MQ=255)
                                                                                                                                             aGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCt                            >  1:1858019/1‑127 (MQ=255)
                                                                                                                                                tataGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCa  >  2:1350469/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCA  >  CP009273/102589‑102882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑