Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,169,139 G→T 7.2% T94T (ACC→ACA)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,169,1390GT 7.2% 1125.9 / 36.7 319T94T (ACC→ACA) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (146/149);  new base T (9/14);  total (156/163)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 325 total aligned reads displayed.

TGGGTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAAT  >  CP009273/1168990‑1169288
                                                                                                                                                     |                                                                                                                                                     
  ggTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAgtgt                                                                                                                                                     >  1:3658318/1‑150 (MQ=255)
   gTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTg                                                                                                                                                    <  1:2519789/150‑1 (MQ=255)
     tCGTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGa                                                                                                                                                  <  2:2086188/150‑1 (MQ=255)
       gTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGaaa                                                                                                                                                <  2:4398370/150‑1 (MQ=255)
        tAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACTATCTGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAg                                                                                                                                               <  1:2017886/150‑1 (MQ=255)
        tAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAg                                                                                                                                               >  1:324234/1‑150 (MQ=255)
        tAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAg                                                                                                                                               <  2:571429/150‑1 (MQ=255)
        tAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAg                                                                                                                                               <  2:615834/150‑1 (MQ=255)
        tAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAg                                                                                                                                               >  1:4900441/1‑150 (MQ=255)
         aaTGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAgg                                                                                                                                              >  2:1217368/1‑150 (MQ=255)
         aaTGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAgg                                                                                                                                              >  1:986922/1‑150 (MQ=255)
           tGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGgcg                                                                                                                                            >  1:3968948/1‑150 (MQ=255)
            gCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGgcgc                                                                                                                                           >  1:2885456/1‑150 (MQ=255)
              aTCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCga                                                                                                                                         <  1:3566273/150‑1 (MQ=255)
              aTCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCga                                                                                                                                         >  2:3363978/1‑150 (MQ=255)
              aTCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTCGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGGAAAGGGTGGAAAGGCGCga                                                                                                                                         >  2:779758/1‑150 (MQ=255)
                ctcGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgagg                                                                                                                                       >  1:4503888/1‑150 (MQ=255)
                 tcGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgagga                                                                                                                                      <  1:4653770/150‑1 (MQ=255)
                  cGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgaggag                                                                                                                                     >  2:1059056/1‑150 (MQ=255)
                     gACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTCGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATa                                                                                                                                  >  2:2809700/1‑150 (MQ=255)
                      aCAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATaa                                                                                                                                 <  1:3389943/150‑1 (MQ=255)
                         ggCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGGATTAACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAATGGTGGAAAGGCGCGAGGAGATAATGt                                                                                                                              >  2:4969346/1‑150 (MQ=255)
                            gcTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCt                                                                                                                           >  2:2763365/1‑150 (MQ=255)
                            gcTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCAGAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCt                                                                                                                           <  2:3784304/150‑1 (MQ=255)
                             cTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtg                                                                                                                          >  2:4563182/1‑150 (MQ=255)
                              tGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtga                                                                                                                         >  1:2858335/1‑150 (MQ=255)
                                aCCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtgatg                                                                                                                       <  1:4994219/150‑1 (MQ=255)
                                aCCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtgatg                                                                                                                       <  1:4102075/150‑1 (MQ=255)
                                  cTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAg                                                                                                                     >  1:1137363/1‑150 (MQ=255)
                                  cTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAg                                                                                                                     >  1:4849681/1‑150 (MQ=255)
                                   ttttttCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAgg                                                                                                                    <  2:4167611/150‑1 (MQ=255)
                                        tcatcaCCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGaaa                                                                                                               >  2:143413/1‑150 (MQ=255)
                                          atcaCCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGGATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGaaaaa                                                                                                             <  2:3064901/150‑1 (MQ=255)
                                           tcaCCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAAc                                                                                                            <  1:188732/150‑1 (MQ=255)
                                            caCCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACt                                                                                                           >  1:4166283/1‑150 (MQ=255)
                                              ccAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGt                                                                                                         >  2:3113995/1‑150 (MQ=255)
                                                  cgcATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTa                                                                                                     >  1:2943171/1‑150 (MQ=255)
                                                  cgcATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTa                                                                                                     <  2:3485717/150‑1 (MQ=255)
                                                    cATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAgg                                                                                                   >  2:517897/1‑150 (MQ=255)
                                                     aTGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAggg                                                                                                  <  2:2477841/150‑1 (MQ=255)
                                                         ccGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGTGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAaga                                                                                              <  2:646631/150‑1 (MQ=255)
                                                         ccGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAaga                                                                                              <  1:1834745/150‑1 (MQ=255)
                                                           gTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGACGAGt                                                                                            >  2:4052790/1‑150 (MQ=255)
                                                                gaAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTccc                                                                                       >  2:3757718/1‑150 (MQ=255)
                                                                 aaaaCTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCa                                                                                      <  2:1691045/150‑1 (MQ=255)
                                                                  aaaCTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAg                                                                                     >  1:3928812/1‑150 (MQ=255)
                                                                    aCTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTc                                                                                   <  1:5130895/150‑1 (MQ=255)
                                                                      tgtgtgGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCTGTAGCTGGTAAAGGGAGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTcag                                                                                 >  2:3375921/1‑148 (MQ=255)
                                                                      tgtgtgGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCg                                                                                 <  1:810917/150‑1 (MQ=255)
                                                                          tgGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAg                                                                             >  2:260314/1‑150 (MQ=255)
                                                                          tgGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAg                                                                             <  2:2697201/150‑1 (MQ=255)
                                                                              cAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATtc                                                                         <  2:4283416/150‑1 (MQ=255)
                                                                              cAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATtc                                                                         <  1:3781410/150‑1 (MQ=255)
                                                                               aaaCGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGTGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATtca                                                                        >  1:37475/1‑150 (MQ=255)
                                                                               aaaCGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATtca                                                                        <  2:2176179/150‑1 (MQ=255)
                                                                               aaaCGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATtca                                                                        >  1:4562872/1‑150 (MQ=255)
                                                                                   gcgcTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCAc                                                                    <  1:4350331/150‑1 (MQ=255)
                                                                                   gcgcTGCATAAGCGTATTAACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGAGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTATGGAAGAGTTTCCCAGTCAGCCAGATTCATCAc                                                                    >  1:5091045/1‑150 (MQ=255)
                                                                                     gcTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCa                                                                  >  2:4320282/1‑150 (MQ=255)
                                                                                     gcTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCa                                                                  <  1:1626990/150‑1 (MQ=255)
                                                                                       tGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCAtt                                                                <  1:848714/150‑1 (MQ=255)
                                                                                        gCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCAttt                                                               <  2:4726991/150‑1 (MQ=255)
                                                                                        gCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCAttt                                                               <  2:3011666/150‑1 (MQ=255)
                                                                                        gCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCAttt                                                               <  2:2885456/150‑1 (MQ=255)
                                                                                         cATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                              <  2:4617296/150‑1 (MQ=255)
                                                                                         cATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                              >  2:1290225/1‑150 (MQ=255)
                                                                                         cATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                              >  1:751942/1‑150 (MQ=255)
                                                                                           tAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGTGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGAt                                                            <  2:190784/150‑1 (MQ=255)
                                                                                           tAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGAt                                                            >  2:1016570/1‑150 (MQ=255)
                                                                                             aGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCg                                                          >  1:2344727/1‑150 (MQ=255)
                                                                                                      aCCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTgg                                                 <  2:4263071/150‑1 (MQ=255)
                                                                                                        cGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCt                                               <  1:1099105/150‑1 (MQ=255)
                                                                                                        cGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCt                                               <  1:4108851/150‑1 (MQ=255)
                                                                                                          gAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGAGACATCACCATTTGATCGGTAAACTGGCTGa                                             >  1:4664049/1‑150 (MQ=255)
                                                                                                            aCAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGTGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGAtt                                           <  2:3658318/150‑1 (MQ=255)
                                                                                                            aCAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCTGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGAtt                                           <  1:1059056/150‑1 (MQ=255)
                                                                                                             cAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGAttt                                          >  1:1161969/1‑150 (MQ=255)
                                                                                                              aaTCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTtc                                         >  1:2843820/1‑150 (MQ=255)
                                                                                                                tCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTtcat                                       >  1:1001179/1‑150 (MQ=255)
                                                                                                                      cGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCa                                 >  2:3981571/1‑150 (MQ=255)
                                                                                                                       gCCACGCTGCCTCGTCGGTAGCTGGTCAAGGGTGGACAGGCGCGAGGAGCTCATGTCCTGAGGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGCGCGGTAAACTGGCTGAGGTCATCATGCac                                >  2:4824888/1‑149 (MQ=255)
                                                                                                                         cACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAAc                              >  2:165364/1‑150 (MQ=255)
                                                                                                                           cGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGc                            >  1:2577955/1‑150 (MQ=255)
                                                                                                                            gCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCa                           >  2:3805752/1‑150 (MQ=255)
                                                                                                                              tGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCaga                         <  2:4489146/150‑1 (MQ=255)
                                                                                                                                 aTCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCa                      <  1:3489780/150‑1 (MQ=255)
                                                                                                                                  tcgtcgGGAGCTGGTAACGGGTGGAAAGGCGCGAGGAGATAAGGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCAt                     <  2:3080978/150‑1 (MQ=255)
                                                                                                                                      cgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTg                 <  2:5108283/150‑1 (MQ=255)
                                                                                                                                      cgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTg                 >  2:83014/1‑150 (MQ=255)
                                                                                                                                       ggTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGc                >  2:356931/1‑150 (MQ=255)
                                                                                                                                         tAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCat              >  2:1965380/1‑150 (MQ=255)
                                                                                                                                         tAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCat              >  1:3041507/1‑150 (MQ=255)
                                                                                                                                         tAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCat              <  1:193575/150‑1 (MQ=255)
                                                                                                                                          aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCata             >  2:3236169/1‑150 (MQ=255)
                                                                                                                                          aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCata             >  2:230828/1‑150 (MQ=255)
                                                                                                                                           gCTGGTAAAGTGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCatat            <  1:578104/150‑1 (MQ=255)
                                                                                                                                             tGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCt          >  1:269756/1‑150 (MQ=255)
                                                                                                                                             tGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCt          >  1:2618650/1‑150 (MQ=255)
                                                                                                                                                tAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGt       <  1:1016613/150‑1 (MQ=255)
                                                                                                                                                 aaaGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTg      <  1:4368111/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGGTTCGTAATGCATCTCGTGACAGGCGCTGACCTTTTTTCATCACCAGCGCATGACCGTGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAAT  >  CP009273/1168990‑1169288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑