Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,716 G→T 80.2% R565S (CGC→AGC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,7160GT80.2% 416.0 / 124.1 217R565S (CGC→AGC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (23/20);  new base T (77/97);  total (100/117)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 217 total aligned reads displayed.

GCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAA  >  CP009273/1249569‑1249862
                                                                                                                                                   |                                                                                                                                                  
gCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGa                                                                                                                                                  <  2:4794148/150‑1 (MQ=255)
 cTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                                 >  1:1997014/1‑150 (MQ=255)
  tCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATa                                                                                                                                                >  2:1149145/1‑150 (MQ=255)
     cAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGcc                                                                                                                                             <  1:4152934/150‑1 (MQ=255)
      aGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCa                                                                                                                                            >  1:2696595/1‑150 (MQ=255)
      aGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCa                                                                                                                                            >  2:4640301/1‑150 (MQ=255)
         tAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATa                                                                                                                                         <  2:3120367/150‑1 (MQ=255)
          aGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATAc                                                                                                                                        <  1:4738559/150‑1 (MQ=255)
           gTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACg                                                                                                                                       >  1:1918195/1‑150 (MQ=255)
             gCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTAAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTg                                                                                                                                     <  2:1144017/150‑1 (MQ=255)
              ccATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                    >  2:2245474/1‑150 (MQ=255)
                  ttttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTAc                                                                                                                                >  1:889747/1‑150 (MQ=255)
                    ttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCa                                                                                                                              >  1:2974978/1‑150 (MQ=255)
                      aCGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGc                                                                                                                            <  1:1416591/150‑1 (MQ=255)
                       cGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCa                                                                                                                           >  2:1656744/1‑150 (MQ=255)
                        gCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCac                                                                                                                          <  2:3689362/150‑1 (MQ=255)
                         cacaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCact                                                                                                                         >  2:4157753/1‑150 (MQ=255)
                          acaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCacta                                                                                                                        <  2:3786142/150‑1 (MQ=255)
                               gTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGAGATTACCAGCACTACTccc                                                                                                                   >  1:4395862/1‑150 (MQ=255)
                                 aCAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGc                                                                                                                 >  2:2755293/1‑150 (MQ=255)
                                  cAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCt                                                                                                                <  2:3334193/150‑1 (MQ=255)
                                   aGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                               >  1:1913298/1‑150 (MQ=255)
                                     atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                             >  1:4221836/1‑150 (MQ=255)
                                       atCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGatat                                                                                                           >  2:72038/1‑150 (MQ=255)
                                         ccccccAACTCAACCTGAGAATACGCGCTGAACGTGGCAGCTCCGTCAGTGGTTTGTACCACTGTCAGAACTTCATTTCCCGTTTTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  1:4413568/150‑1 (MQ=255)
                                         ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  2:2892497/150‑1 (MQ=255)
                                         ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  2:2024504/150‑1 (MQ=255)
                                           ccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCaa                                                                                                       >  2:3934470/1‑150 (MQ=255)
                                             ccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATa                                                                                                     >  1:4673763/1‑150 (MQ=255)
                                               aaCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaaa                                                                                                   <  2:316553/150‑1 (MQ=255)
                                                 cTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATc                                                                                                 <  2:2433129/150‑1 (MQ=255)
                                                    aaCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATGTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcccc                                                                                              <  1:3309620/150‑1 (MQ=255)
                                                      ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCtt                                                                                            <  2:4867782/150‑1 (MQ=255)
                                                            aagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattat                                                                                      <  2:3076792/150‑1 (MQ=255)
                                                              gaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattatta                                                                                    <  2:1107248/150‑1 (MQ=255)
                                                                cgcgCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAAc                                                                                  >  2:3821816/1‑150 (MQ=255)
                                                                  cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                >  2:1906376/1‑150 (MQ=255)
                                                                    cTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                              <  1:2440117/150‑1 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             >  1:3862360/1‑150 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             >  2:4484460/1‑150 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             <  1:3991679/150‑1 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            >  1:214554/1‑150 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            <  1:1223669/150‑1 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            <  2:1754872/150‑1 (MQ=255)
                                                                         cGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAcc                                                                         <  1:1719616/150‑1 (MQ=255)
                                                                          gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                        >  1:3106286/1‑150 (MQ=255)
                                                                           aGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCt                                                                       <  2:3257041/150‑1 (MQ=255)
                                                                            ggCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTc                                                                      <  2:5136921/150‑1 (MQ=255)
                                                                               cgcgCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                   >  1:1057835/1‑150 (MQ=255)
                                                                                gcgcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCca                                                                  <  2:2069093/150‑1 (MQ=255)
                                                                                  gcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                <  2:2402380/150‑1 (MQ=255)
                                                                                    cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                              >  1:506045/1‑150 (MQ=255)
                                                                                    cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                              >  1:3559443/1‑150 (MQ=255)
                                                                                     gTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                             >  1:3922712/1‑150 (MQ=255)
                                                                                      tCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAacaaca                                                            >  1:3604307/1‑150 (MQ=255)
                                                                                             tttatACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAc                                                     <  1:4820479/150‑1 (MQ=255)
                                                                                                ttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCa                                                  >  1:3779631/1‑150 (MQ=255)
                                                                                                 tACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat                                                 <  1:106399/150‑1 (MQ=255)
                                                                                                       tGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                           <  2:1456758/150‑1 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  1:876240/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATCATAAAgg                                         >  2:2406400/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  2:1395835/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  2:4970479/1‑150 (MQ=255)
                                                                                                          cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt                                        >  1:1485806/1‑150 (MQ=255)
                                                                                                           aGAACTTCAGTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                       <  2:1550250/150‑1 (MQ=255)
                                                                                                                ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                                  >  1:687651/1‑150 (MQ=255)
                                                                                                                ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                                  <  1:4640301/150‑1 (MQ=255)
                                                                                                                 tCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa                                 >  2:4032732/1‑150 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               <  1:2245474/150‑1 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               <  2:1492119/150‑1 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               >  2:4122494/1‑150 (MQ=255)
                                                                                                                    tttCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCg                              >  2:1289149/1‑150 (MQ=255)
                                                                                                                     ttCCCGTTGTGGCCTCGCTGCCCTGGTTGCTTATAGCCATTACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCATCGCCAACAACATCAGCAAGCATAATAAAGGTACTGTTACCGc                             <  2:1239749/150‑1 (MQ=255)
                                                                                                                       cccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc                           >  1:4051677/1‑150 (MQ=255)
                                                                                                                        ccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa                          <  2:3272231/150‑1 (MQ=255)
                                                                                                                         cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                         <  1:881782/150‑1 (MQ=255)
                                                                                                                         cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAATGTTACCGCTCAg                         >  1:723471/1‑150 (MQ=255)
                                                                                                                          gTTGTGGCCTCGTTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg                        <  1:866969/150‑1 (MQ=255)
                                                                                                                            tgtgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt                      <  2:889060/150‑1 (MQ=255)
                                                                                                                            tgtgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt                      <  2:1172824/150‑1 (MQ=255)
                                                                                                                             gtgGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtc                     <  1:3436468/150‑1 (MQ=255)
                                                                                                                              tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                    <  2:585438/150‑1 (MQ=255)
                                                                                                                              tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                    <  2:2696957/150‑1 (MQ=255)
                                                                                                                               ggCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                   <  1:259852/150‑1 (MQ=255)
                                                                                                                               ggCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                   >  2:1109677/1‑150 (MQ=255)
                                                                                                                                gCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTctct                  <  1:4856756/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  1:4587105/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  2:4674043/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  2:2970586/150‑1 (MQ=255)
                                                                                                                                  cTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc                >  2:2571585/1‑150 (MQ=255)
                                                                                                                                   tCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg               >  1:643856/1‑150 (MQ=255)
                                                                                                                                   tCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg               >  1:4334516/1‑150 (MQ=255)
                                                                                                                                     gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt             >  1:1085703/1‑150 (MQ=255)
                                                                                                                                     gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt             >  1:4369609/1‑150 (MQ=255)
                                                                                                                                         cccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt         <  1:4688580/150‑1 (MQ=255)
                                                                                                                                          ccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg        <  2:5064714/150‑1 (MQ=255)
                                                                                                                                            tGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg      >  2:4318065/1‑150 (MQ=255)
                                                                                                                                             ggTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg     <  2:3648060/150‑1 (MQ=255)
                                                                                                                                               ttGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCa   >  1:3170277/1‑150 (MQ=255)
                                                                                                                                                tGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaa  <  2:3559443/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAA  >  CP009273/1249569‑1249862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑