Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,257,232 G→T 100% R34S (CGC→AGC)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,257,2320GT98.1% 853.4 / 4.8 269R34S (CGC→AGC) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (147/117);  total (148/121)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257085‑1257378
                                                                                                                                                   |                                                                                                                                                  
    ggAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGctacctac                                                                                                                                              <  1:4392973/150‑1 (MQ=255)
     gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                             <  1:4577474/150‑1 (MQ=255)
      aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                            <  1:3309956/150‑1 (MQ=255)
       aGCACGACGCAGGGCATCAACCATAACGCCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgc                                                                                                                                           <  2:409243/150‑1 (MQ=255)
       aGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgc                                                                                                                                           <  1:376397/150‑1 (MQ=255)
        gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcg                                                                                                                                          <  2:1526363/150‑1 (MQ=255)
         cACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                         <  2:2892687/150‑1 (MQ=255)
            gacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGt                                                                                                                                      >  2:2379939/1‑150 (MQ=255)
            gacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGt                                                                                                                                      >  2:3125010/1‑150 (MQ=255)
             acgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTc                                                                                                                                     <  1:92714/150‑1 (MQ=255)
              cgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                    >  2:1723408/1‑150 (MQ=255)
              cgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                    >  2:3328905/1‑150 (MQ=255)
                     cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTg                                                                                                                             >  1:3530561/1‑150 (MQ=255)
                       tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGaa                                                                                                                           >  2:5075545/1‑150 (MQ=255)
                          aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                        <  2:2081394/150‑1 (MQ=255)
                          aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                        >  2:2746673/1‑150 (MQ=255)
                           ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtgt                                                                                                                       <  2:5110285/150‑1 (MQ=255)
                               aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                   <  2:5033163/150‑1 (MQ=255)
                               aaCGACTAATTCCATCAGGTTGTCGGTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATATTCATTAATTTGTACGCTGACTAAGCCAACGCTAAAGATACATACACCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                   >  1:3652122/1‑150 (MQ=255)
                                  gACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                                >  2:4640176/1‑150 (MQ=255)
                                   aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                               >  2:3453619/1‑150 (MQ=255)
                                   aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTAAGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                               >  1:3573568/1‑150 (MQ=255)
                                     tAAGTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                             <  2:2963351/150‑1 (MQ=255)
                                       tttCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                           >  2:4527133/2‑150 (MQ=255)
                                       aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                           >  1:4823903/1‑150 (MQ=255)
                                         tCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                         <  1:4362972/150‑1 (MQ=255)
                                           cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                       >  1:2764546/1‑150 (MQ=255)
                                            aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATa                                                                                                      <  1:5148168/150‑1 (MQ=255)
                                                ggTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                                  <  1:1616315/150‑1 (MQ=255)
                                                  ttGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTtgt                                                                                                <  2:1783980/150‑1 (MQ=255)
                                                       gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                           >  2:1248485/1‑150 (MQ=255)
                                                           gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTcc                                                                                       <  1:1801684/150‑1 (MQ=255)
                                                            tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                                      >  1:572820/1‑150 (MQ=255)
                                                             agGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgg                                                                                     >  2:2338746/1‑150 (MQ=255)
                                                             agGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgg                                                                                     >  2:3723201/1‑150 (MQ=255)
                                                               gggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgggg                                                                                   <  2:2296551/150‑1 (MQ=255)
                                                               gggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgggg                                                                                   <  1:3098645/150‑1 (MQ=255)
                                                                ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGt                                                                                  <  1:2162342/150‑1 (MQ=255)
                                                                 gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                                 >  2:1993149/1‑150 (MQ=255)
                                                                 gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                                 >  2:6120/1‑150 (MQ=255)
                                                                   acaAGTGTACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGc                                                                               >  2:265329/1‑150 (MQ=255)
                                                                    caAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                              >  1:3175967/1‑150 (MQ=255)
                                                                     aaGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGt                                                                             >  2:956265/1‑150 (MQ=255)
                                                                      aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGtt                                                                            <  1:4021721/150‑1 (MQ=255)
                                                                       gTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTa                                                                           >  2:1494097/1‑150 (MQ=255)
                                                                         ggACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAcc                                                                         >  2:1519468/1‑150 (MQ=255)
                                                                          gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCa                                                                        <  2:4935399/150‑1 (MQ=255)
                                                                           aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                       <  1:808139/150‑1 (MQ=255)
                                                                           aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                       <  1:1929639/150‑1 (MQ=255)
                                                                            cTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGc                                                                      >  2:4020672/1‑150 (MQ=255)
                                                                            cTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGc                                                                      >  2:466459/1‑150 (MQ=255)
                                                                              ggATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaa                                                                    >  1:1078916/1‑150 (MQ=255)
                                                                               gatgatGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                                   >  2:3375894/1‑150 (MQ=255)
                                                                                  gatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                                >  2:3292764/1‑150 (MQ=255)
                                                                                  gatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                                >  2:710521/1‑150 (MQ=255)
                                                                                    tgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCt                                                              <  2:84133/150‑1 (MQ=255)
                                                                                         atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                         >  1:4162857/1‑150 (MQ=255)
                                                                                         atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                         <  2:1132729/150‑1 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  1:4917661/1‑150 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  1:1752242/1‑150 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTACAGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  2:4148487/1‑150 (MQ=255)
                                                                                             caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                     >  1:1868406/1‑150 (MQ=255)
                                                                                             caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                     >  1:3692005/1‑150 (MQ=255)
                                                                                              accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAgg                                                    <  2:1664000/150‑1 (MQ=255)
                                                                                                 accGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                                 >  1:5038469/1‑150 (MQ=255)
                                                                                                  ccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACg                                                <  1:541640/150‑1 (MQ=255)
                                                                                                    gcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgag                                              >  1:2375413/1‑150 (MQ=255)
                                                                                                     cgTACATTGTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                             <  1:2682589/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            >  2:3888239/1‑150 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  1:1187901/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  1:3590622/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  2:2163798/150‑1 (MQ=255)
                                                                                                       tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                           <  2:5116885/150‑1 (MQ=255)
                                                                                                        aCATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaaga                                          <  1:131763/150‑1 (MQ=255)
                                                                                                              tCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCa                                    <  1:1396872/150‑1 (MQ=255)
                                                                                                              tCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCa                                    <  1:2078024/150‑1 (MQ=255)
                                                                                                                aTTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAgg                                  <  1:1324463/150‑1 (MQ=255)
                                                                                                                    aTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAGGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                              >  2:757651/1‑150 (MQ=255)
                                                                                                                        gTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTc                          >  1:3610754/1‑150 (MQ=255)
                                                                                                                           cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt                       >  1:4106475/1‑150 (MQ=255)
                                                                                                                           cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGGTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt                       >  2:4618134/1‑150 (MQ=255)
                                                                                                                            gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAtt                      <  1:4280676/150‑1 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:1124441/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:1263873/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     <  2:2562983/150‑1 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:3538343/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCGACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     <  2:4824037/150‑1 (MQ=255)
                                                                                                                              tGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGATCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtgg                    >  1:4619872/1‑150 (MQ=255)
                                                                                                                               gACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                   <  1:2591265/150‑1 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 >  2:1824999/1‑150 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 >  1:2487325/1‑150 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 <  2:2366856/150‑1 (MQ=255)
                                                                                                                                       cATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa           >  1:471415/1‑150 (MQ=255)
                                                                                                                                       cATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa           >  2:4192532/1‑150 (MQ=255)
                                                                                                                                          cacTACAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTGACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCt        <  2:275822/148‑1 (MQ=255)
                                                                                                                                            cTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGc      <  2:1893619/150‑1 (MQ=255)
                                                                                                                                              aaaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCg    <  2:3651697/150‑1 (MQ=255)
                                                                                                                                               aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGa   >  2:4752730/1‑150 (MQ=255)
                                                                                                                                               aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGa   >  1:911988/1‑150 (MQ=255)
                                                                                                                                                aGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGaa  <  1:703620/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257085‑1257378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑