Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,284,857 G→T 100% D53Y (GAT→TAT)  rssA → putative patatin‑like family phospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,284,8570GT95.7% 650.1 / 17.3 223D53Y (GAT→TAT) rssAputative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base T (107/106);  total (114/109)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 233 total aligned reads displayed.

ATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGA  >  CP009273/1284710‑1285002
                                                                                                                                                   |                                                                                                                                                 
aTAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctat                                                                                                                                                 <  2:4871668/150‑1 (MQ=255)
 tAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatc                                                                                                                                                <  2:1873379/150‑1 (MQ=255)
   aaaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcga                                                                                                                                              >  1:4561606/1‑150 (MQ=255)
    aaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                             >  1:5024528/1‑150 (MQ=255)
      aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTa                                                                                                                                           <  2:5062268/150‑1 (MQ=255)
      aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTa                                                                                                                                           <  2:4208719/150‑1 (MQ=255)
      aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTa                                                                                                                                           <  1:2937482/150‑1 (MQ=255)
       tAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAt                                                                                                                                          >  1:2122151/1‑150 (MQ=255)
        aGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATc                                                                                                                                         >  1:789319/1‑150 (MQ=255)
         gggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCt                                                                                                                                        <  2:3445289/150‑1 (MQ=255)
          ggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTg                                                                                                                                       <  2:2917346/150‑1 (MQ=255)
           gCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTgc                                                                                                                                      >  1:4030468/1‑150 (MQ=255)
            cTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGAGATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTgcg                                                                                                                                     <  1:2243083/150‑1 (MQ=255)
               gcgcTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTg                                                                                                                                  <  1:350615/150‑1 (MQ=255)
                cgcTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTgg                                                                                                                                 >  1:1846493/1‑150 (MQ=255)
                 gcTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGa                                                                                                                                >  2:4733704/1‑150 (MQ=255)
                  cTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGaa                                                                                                                               <  2:2905250/150‑1 (MQ=255)
                  cTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGAGATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGaa                                                                                                                               <  2:3945135/150‑1 (MQ=255)
                   tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                              >  2:3176854/1‑150 (MQ=255)
                   tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                              >  2:1937539/1‑150 (MQ=255)
                   tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                              <  2:2885787/150‑1 (MQ=255)
                   tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAAAGCGCTAAAAAAAGTGGGTATAGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTCGCGATTATCTGCGCTGGAAg                                                                                                                              >  1:336170/1‑150 (MQ=255)
                       aTCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTTAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTg                                                                                                                          >  2:1556397/1‑150 (MQ=255)
                          tGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGt                                                                                                                       >  2:3550310/1‑150 (MQ=255)
                            gcgcGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGa                                                                                                                     >  1:1059089/1‑150 (MQ=255)
                               cggcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                  <  2:1504885/150‑1 (MQ=255)
                               cggcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                  <  2:47774/150‑1 (MQ=255)
                               cggcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                  <  1:4284134/150‑1 (MQ=255)
                               cggcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                  >  2:4289534/1‑150 (MQ=255)
                                  cgAGAGGTTGGTCGCATATTTGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCtt                                                                                                               <  1:2708618/150‑1 (MQ=255)
                                  cgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCtt                                                                                                               <  2:3559198/150‑1 (MQ=255)
                                  cgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCtt                                                                                                               >  1:2604567/1‑150 (MQ=255)
                                    agagGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATGATCTGCGCTGGAAGATTGGGTGACCTCTTTc                                                                                                             <  2:3704944/150‑1 (MQ=255)
                                     gagGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCa                                                                                                            <  2:4358897/150‑1 (MQ=255)
                                     gagGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCa                                                                                                            <  2:5043055/150‑1 (MQ=255)
                                      agGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAg                                                                                                           <  1:2547605/150‑1 (MQ=255)
                                      agGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAg                                                                                                           <  1:5149660/150‑1 (MQ=255)
                                         ttGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTa                                                                                                        >  2:779262/1‑150 (MQ=255)
                                          tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTAt                                                                                                       >  2:4463046/1‑150 (MQ=255)
                                              cGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg                                                                                                   >  1:4757152/1‑150 (MQ=255)
                                               gCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGa                                                                                                  <  2:3877202/150‑1 (MQ=255)
                                                 atatTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATg                                                                                                >  1:597877/1‑150 (MQ=255)
                                                  tatTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGt                                                                                               <  2:260126/150‑1 (MQ=255)
                                                      ggCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTa                                                                                           >  1:2455597/1‑150 (MQ=255)
                                                      ggCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTa                                                                                           <  2:1223317/150‑1 (MQ=255)
                                                       gCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTAc                                                                                          <  2:3097437/150‑1 (MQ=255)
                                                        cGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACg                                                                                         >  1:5095034/1‑150 (MQ=255)
                                                        cGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACg                                                                                         >  1:2538778/1‑150 (MQ=255)
                                                              taATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGAt                                                                                   <  1:3977635/150‑1 (MQ=255)
                                                              taATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGAt                                                                                   >  1:1271332/1‑150 (MQ=255)
                                                                   cgcTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtc                                                                              >  1:1779249/1‑150 (MQ=255)
                                                                       aaaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctctc                                                                          >  2:1702374/1‑150 (MQ=255)
                                                                         aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCt                                                                        <  1:4958867/150‑1 (MQ=255)
                                                                         aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCt                                                                        <  1:1943613/150‑1 (MQ=255)
                                                                            aaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGc                                                                     <  1:1552664/150‑1 (MQ=255)
                                                                             aGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCa                                                                    >  1:2402653/1‑150 (MQ=255)
                                                                              gTGGGTATTGAAATTGATATCGTTTCATGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGATGGAAGATTGTGTGACCTCTTTCAGTTATTTGGATGTTTTACGCCTTATGGATCTCTCCTGGCAg                                                                   <  1:5108851/150‑1 (MQ=255)
                                                                               tGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTCTTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAgc                                                                  >  1:4403719/1‑150 (MQ=255)
                                                                                 ggTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAgcgc                                                                >  1:2648137/1‑150 (MQ=255)
                                                                                   tATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCgg                                                              >  1:84013/1‑150 (MQ=255)
                                                                                       gAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTggg                                                          >  2:1062663/1‑150 (MQ=255)
                                                                                        aaaTTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGt                                                         >  2:2154890/1‑150 (MQ=255)
                                                                                              atatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgc                                                   >  1:4694727/1‑150 (MQ=255)
                                                                                              atatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGAGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgc                                                   >  2:1455841/1‑150 (MQ=255)
                                                                                               tatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgcg                                                  <  1:3176854/150‑1 (MQ=255)
                                                                                               tatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgcg                                                  >  2:576423/1‑150 (MQ=255)
                                                                                                atCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgcgc                                                 >  1:3891909/1‑150 (MQ=255)
                                                                                                  cGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCgcgg                                               >  2:2028013/1‑150 (MQ=255)
                                                                                                   gtggCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCgcggc                                              <  1:99366/147‑1 (MQ=255)
                                                                                                     tGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTGTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGa                                            <  1:4423479/150‑1 (MQ=255)
                                                                                                       cAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                          >  2:2193967/1‑150 (MQ=255)
                                                                                                       cAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                          >  1:1501460/1‑150 (MQ=255)
                                                                                                       cAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                          <  1:2101030/150‑1 (MQ=255)
                                                                                                       cAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                          <  2:3773550/150‑1 (MQ=255)
                                                                                                        aGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCg                                         >  2:1626191/1‑150 (MQ=255)
                                                                                                        aGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCg                                         <  2:3474/150‑1 (MQ=255)
                                                                                                         ggATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgt                                        >  2:4066011/1‑150 (MQ=255)
                                                                                                             gTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCt                                    <  2:4561606/150‑1 (MQ=255)
                                                                                                              ttCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCtt                                   <  2:3413264/150‑1 (MQ=255)
                                                                                                                cAATTGGTTCGCTGGTGGGCGCTGCCTAGGCATGCTATCGAGTATCTGCGCTGGCAGAGTGGGTGAGCTCTTTCAGTTATTGGGCTGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtca                                 <  2:4011649/150‑1 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatc                              >  1:1794915/1‑150 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatc                              >  2:3320620/1‑150 (MQ=255)
                                                                                                                   gtGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGTAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatc                              <  2:1462316/149‑1 (MQ=255)
                                                                                                                     ggTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatcaa                            <  1:726267/150‑1 (MQ=255)
                                                                                                                     ggTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatcaa                            >  1:2124520/1‑150 (MQ=255)
                                                                                                                      gTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTAGTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTcaatcaat                           >  1:1804295/1‑150 (MQ=255)
                                                                                                                       ttCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATa                          <  1:110195/150‑1 (MQ=255)
                                                                                                                        tCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATAc                         >  2:2644572/1‑150 (MQ=255)
                                                                                                                          gCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACcg                       <  1:3943888/150‑1 (MQ=255)
                                                                                                                            tggtggGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATGGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACcgcg                     >  2:134943/1‑150 (MQ=255)
                                                                                                                                 ggCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaata                <  1:3616066/150‑1 (MQ=255)
                                                                                                                                 ggCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaata                <  1:2177200/150‑1 (MQ=255)
                                                                                                                                   cgcTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaataat              >  1:3648036/1‑150 (MQ=255)
                                                                                                                                      tGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGcc           <  2:1199867/150‑1 (MQ=255)
                                                                                                                                        ccTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCgg         >  1:3738383/1‑150 (MQ=255)
                                                                                                                                           atgcatgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGaaa      <  1:3072494/150‑1 (MQ=255)
                                                                                                                                           atgcatgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGaaa      <  2:2122151/150‑1 (MQ=255)
                                                                                                                                           atgcatgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGaaa      <  2:2382088/150‑1 (MQ=255)
                                                                                                                                               atgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACaga  <  1:4117377/150‑1 (MQ=255)
                                                                                                                                               atgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACaga  <  2:630412/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGA  >  CP009273/1284710‑1285002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑