Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 103,352 C→A 84.6% I103I (ATC→ATA)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273103,3520CA84.6% 552.1 / 112.1 260I103I (ATC→ATA) lpxCUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base C (18/22);  new base A (97/123);  total (115/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 264 total aligned reads displayed.

CAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTT  >  CP009273/103205‑103501
                                                                                                                                                   |                                                                                                                                                     
cAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCAt                                                                                                                                                     >  2:1373559/1‑150 (MQ=255)
cAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAAt                                                                                                                                                     >  1:2697555/1‑150 (MQ=255)
  aaTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATgg                                                                                                                                                   >  1:2927238/1‑150 (MQ=255)
   aTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGa                                                                                                                                                  <  1:2981739/150‑1 (MQ=255)
     cTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACg                                                                                                                                                >  2:1142629/1‑150 (MQ=255)
       gtgCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGc                                                                                                                                              <  2:820447/150‑1 (MQ=255)
        tgCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCa                                                                                                                                             >  1:1969713/1‑150 (MQ=255)
         gCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAg                                                                                                                                            <  2:2968359/150‑1 (MQ=255)
           gTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAgcg                                                                                                                                          >  1:4644859/1‑150 (MQ=255)
            tGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAgcgc                                                                                                                                         >  1:1284215/1‑150 (MQ=255)
               tACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGcgccgc                                                                                                                                      >  2:897227/1‑150 (MQ=255)
                  cATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTcc                                                                                                                                   >  1:4442525/1‑150 (MQ=255)
                  cATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTcc                                                                                                                                   >  1:1762123/1‑150 (MQ=255)
                   aTGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCg                                                                                                                                  >  1:415520/1‑150 (MQ=255)
                   aTGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCg                                                                                                                                  <  1:723790/150‑1 (MQ=255)
                    tGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGt                                                                                                                                 >  1:431471/1‑150 (MQ=255)
                      ctctGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGttt                                                                                                                               <  2:1661985/150‑1 (MQ=255)
                       tctGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTg                                                                                                                              >  2:2615456/1‑150 (MQ=255)
                         tGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGta                                                                                                                            <  1:4580056/150‑1 (MQ=255)
                           tACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGtata                                                                                                                          <  1:1634814/150‑1 (MQ=255)
                              gtgtCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACct                                                                                                                       <  2:757799/150‑1 (MQ=255)
                                 tCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGANAATGGACGGCAGCGCCGCTCCGTTTGTATACctgct                                                                                                                    <  1:2680908/150‑1 (MQ=255)
                                 tCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTGATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACctgct                                                                                                                    <  2:2237321/150‑1 (MQ=255)
                                  cTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGAAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACctgctg                                                                                                                   <  1:833790/150‑1 (MQ=255)
                                    ggTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTgctgct                                                                                                                 >  1:3049755/1‑150 (MQ=255)
                                    ggTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTgctgct                                                                                                                 >  2:4347856/1‑150 (MQ=255)
                                     gTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCtt                                                                                                                <  1:1684428/150‑1 (MQ=255)
                                       cAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGa                                                                                                              >  2:1814989/1‑150 (MQ=255)
                                         aCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACg                                                                                                            <  2:1225311/150‑1 (MQ=255)
                                         aCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACg                                                                                                            <  1:4946189/150‑1 (MQ=255)
                                          cGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGc                                                                                                           >  2:34202/1‑150 (MQ=255)
                                          cGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGc                                                                                                           <  2:779439/150‑1 (MQ=255)
                                           gAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGcc                                                                                                          >  1:1021491/1‑150 (MQ=255)
                                            aGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCg                                                                                                         <  1:607912/150‑1 (MQ=255)
                                             gCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCgg                                                                                                        <  2:3368687/150‑1 (MQ=255)
                                               atgatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTa                                                                                                      <  2:621131/150‑1 (MQ=255)
                                               atgatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTa                                                                                                      <  2:3981007/150‑1 (MQ=255)
                                                tgatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTAt                                                                                                     <  2:2707457/150‑1 (MQ=255)
                                                tgatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTAt                                                                                                     <  2:4207731/150‑1 (MQ=255)
                                                 gatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATc                                                                                                    <  2:3556414/150‑1 (MQ=255)
                                                 gatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATc                                                                                                    <  2:4316156/150‑1 (MQ=255)
                                                 gatgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAGTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATc                                                                                                    <  2:4118272/150‑1 (MQ=255)
                                                  atgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcg                                                                                                   >  1:32409/1‑150 (MQ=255)
                                                   tgTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcga                                                                                                  <  1:519086/150‑1 (MQ=255)
                                                    gTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgac                                                                                                 <  2:286588/150‑1 (MQ=255)
                                                    gTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgac                                                                                                 <  2:1798468/150‑1 (MQ=255)
                                                    gTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAAAGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgac                                                                                                 >  1:317804/1‑150 (MQ=255)
                                                     tACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgacg                                                                                                <  1:934731/150‑1 (MQ=255)
                                                       cGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAg                                                                                              >  1:2966579/1‑150 (MQ=255)
                                                           tttCAACCGTAGAGCACCTTAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGa                                                                                          <  2:3841489/150‑1 (MQ=255)
                                                                aCCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTgc                                                                                     >  1:3389870/1‑150 (MQ=255)
                                                                    tAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCa                                                                                 <  1:2810422/150‑1 (MQ=255)
                                                                    tAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCa                                                                                 >  1:3602402/1‑150 (MQ=255)
                                                                            cTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAAttt                                                                         <  1:209814/150‑1 (MQ=255)
                                                                               aaTGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGtt                                                                      <  1:2596926/150‑1 (MQ=255)
                                                                                aTGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTc                                                                     <  2:3770954/150‑1 (MQ=255)
                                                                                  gctgctCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGc                                                                   <  1:4210920/150‑1 (MQ=255)
                                                                                    tgctCTCGCGGGCTTGGGCATCGATCACAGTGTTAGCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCAt                                                                 <  2:3088203/150‑1 (MQ=255)
                                                                                    tgctCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCAt                                                                 <  1:4071797/150‑1 (MQ=255)
                                                                                     gctCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATc                                                                >  1:399156/1‑150 (MQ=255)
                                                                                      ctctCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCa                                                               <  2:1996782/150‑1 (MQ=255)
                                                                                      ctctCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCa                                                               <  1:3036463/150‑1 (MQ=255)
                                                                                           gcgGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAAGTTGTTCGCATCAAagag                                                          >  2:4448548/1‑150 (MQ=255)
                                                                                            cgGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAgaga                                                         >  1:2274586/1‑150 (MQ=255)
                                                                                              ggCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACt                                                       <  1:3789251/150‑1 (MQ=255)
                                                                                              ggCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGACTGTATCGACGAGTTGAACTGCGCCAAAAAATTTTTTCTCATCAAAGAGACt                                                       >  2:4829902/1‑150 (MQ=255)
                                                                                                 ttGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGtt                                                    <  1:2451667/150‑1 (MQ=255)
                                                                                                  tGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTc                                                   <  2:4276691/150‑1 (MQ=255)
                                                                                                   gggCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCg                                                  >  1:5032651/1‑150 (MQ=255)
                                                                                                      cATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCgtgt                                               <  1:1855525/150‑1 (MQ=255)
                                                                                                      cATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCgtgt                                               <  1:1628667/150‑1 (MQ=255)
                                                                                                        tCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCg                                             <  1:2633289/150‑1 (MQ=255)
                                                                                                        tCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCg                                             >  1:1068697/1‑150 (MQ=255)
                                                                                                          gATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGaa                                           >  1:3564794/1‑150 (MQ=255)
                                                                                                             aaCATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGAt                                        <  1:4321256/150‑1 (MQ=255)
                                                                                                             aaCATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGAt                                        >  1:3280569/1‑150 (MQ=255)
                                                                                                                aTTGTTATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGc                                     >  2:3484343/1‑150 (MQ=255)
                                                                                                                     tATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATaa                                <  2:3925731/150‑1 (MQ=255)
                                                                                                                      aTCGAAGTTCACGCGCCGTAAAGCACGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATaat                               <  2:4681014/150‑2 (MQ=255)
                                                                                                                      aTCGAAGTTAACGCGCCGGAAATCCCGATACTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAg                               <  1:1444844/150‑1 (MQ=255)
                                                                                                                      aTCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAg                               <  1:3942105/150‑1 (MQ=255)
                                                                                                                       tCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGt                              <  1:1979452/150‑1 (MQ=255)
                                                                                                                          aaGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTggg                           >  1:3439644/1‑150 (MQ=255)
                                                                                                                           aGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGc                          >  1:3816303/1‑150 (MQ=255)
                                                                                                                            gTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCt                         >  2:5086178/1‑150 (MQ=255)
                                                                                                                               aaCGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGaa                      >  1:4864369/1‑150 (MQ=255)
                                                                                                                                 cgcgCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAtt                    <  2:3171438/150‑1 (MQ=255)
                                                                                                                                 cgcgCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAtt                    >  1:4736333/1‑150 (MQ=255)
                                                                                                                                 cgcgCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAtt                    <  2:2471751/150‑1 (MQ=255)
                                                                                                                                  gcgcCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAttt                   >  2:4063879/1‑150 (MQ=255)
                                                                                                                                       ggAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc              <  2:4934614/150‑1 (MQ=255)
                                                                                                                                        gAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg             <  1:1044536/150‑1 (MQ=255)
                                                                                                                                        gAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg             <  1:2985640/150‑1 (MQ=255)
                                                                                                                                          aaTCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGAGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTa           <  1:2462035/150‑1 (MQ=255)
                                                                                                                                           aTCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTAc          >  1:688798/1‑150 (MQ=255)
                                                                                                                                           aTCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTAc          <  2:1969713/150‑1 (MQ=255)
                                                                                                                                             cccGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACaa        >  2:2511677/1‑150 (MQ=255)
                                                                                                                                               cGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATg      >  2:2771656/1‑150 (MQ=255)
                                                                                                                                                gATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATgg     >  2:159866/1‑150 (MQ=255)
                                                                                                                                                   cATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGttt  <  2:4918036/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTT  >  CP009273/103205‑103501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑