Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,296,729 C→A 100% H431N (CAC→AAC)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,296,7290CA99.3% 918.1 / ‑3.0 278H431N (CAC→AAC) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (130/146);  total (131/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

ACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG  >  CP009273/1296583‑1296875
                                                                                                                                                  |                                                                                                                                                  
aCAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAGACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAcc                                                                                                                                                 <  1:3924577/150‑1 (MQ=255)
aCAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAcc                                                                                                                                                 <  2:4594729/150‑1 (MQ=255)
  aaaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAg                                                                                                                                               <  2:2416252/150‑1 (MQ=255)
  aaaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTACATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAg                                                                                                                                               <  2:1919834/150‑1 (MQ=255)
    aCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAggg                                                                                                                                             >  2:3628501/1‑150 (MQ=255)
     ccGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGt                                                                                                                                            >  1:3336631/1‑150 (MQ=255)
     ccGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGt                                                                                                                                            >  2:2538204/1‑150 (MQ=255)
       gTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAc                                                                                                                                          <  1:3527128/150‑1 (MQ=255)
       gTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAc                                                                                                                                          <  1:3557689/150‑1 (MQ=255)
         tGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtt                                                                                                                                        <  2:4931279/150‑1 (MQ=255)
           aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtttt                                                                                                                                      <  2:3148366/150‑1 (MQ=255)
           aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtttt                                                                                                                                      >  2:2733917/1‑150 (MQ=255)
              aTCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                   >  2:289087/1‑150 (MQ=255)
              aTCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                   <  1:2636473/150‑1 (MQ=255)
               tCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtg                                                                                                                                  <  2:356076/150‑1 (MQ=255)
                 aaCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                                <  2:3559748/150‑1 (MQ=255)
                  aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTgg                                                                                                                               <  2:84117/150‑1 (MQ=255)
                  aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTgg                                                                                                                               >  1:1214900/1‑150 (MQ=255)
                   ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGc                                                                                                                              <  2:1707507/150‑1 (MQ=255)
                     tgttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGccc                                                                                                                            <  1:3611564/150‑1 (MQ=255)
                        tgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTg                                                                                                                         <  1:749051/150‑1 (MQ=255)
                           ataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                      <  1:2619818/150‑1 (MQ=255)
                            taACACCTCCGATCTGCATAAACAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                     <  2:3267240/150‑1 (MQ=255)
                            taACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                     <  1:2069618/150‑1 (MQ=255)
                             aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                    >  1:2289660/1‑150 (MQ=255)
                                  cTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtg                                                                                                               >  2:1167013/1‑150 (MQ=255)
                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              <  1:4886276/150‑1 (MQ=255)
                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              >  1:1382097/1‑150 (MQ=255)
                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              >  2:3354822/1‑150 (MQ=255)
                                    ccGATCTGGTTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGtgtg                                                                                                             <  1:4496189/150‑1 (MQ=255)
                                      gATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCTTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCt                                                                                                           >  2:806929/1‑150 (MQ=255)
                                        tCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGa                                                                                                         >  1:2207672/1‑150 (MQ=255)
                                          tGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACt                                                                                                       >  1:3132427/1‑150 (MQ=255)
                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACttt                                                                                                                                       <  2:3775341/117‑1 (MQ=255)
                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACttt                                                                                                                                       >  1:3775341/1‑117 (MQ=255)
                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      >  1:547285/1‑150 (MQ=255)
                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      >  1:730516/1‑150 (MQ=255)
                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      <  2:730516/150‑1 (MQ=255)
                                            cATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAc                                                                                                     <  1:4005069/150‑1 (MQ=255)
                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  2:3730104/150‑1 (MQ=255)
                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  1:2695651/150‑1 (MQ=255)
                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  2:4487976/150‑1 (MQ=255)
                                               aaaaaGCTTGCTATTGCTTCCTCTTCATTGTTTAATAAAAACATTGGTGTATACGTCATTCTGGTTAACCTGGAGTTGATAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGTCTGGTGTGCTGACTACAAc                                                                                                  <  2:1132460/150‑1 (MQ=255)
                                                  aaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGaa                                                                                               <  2:2205664/150‑1 (MQ=255)
                                                     cTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCa                                                                                            <  2:4092557/150‑1 (MQ=255)
                                                     cTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCa                                                                                            >  1:1169067/1‑150 (MQ=255)
                                                      tGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCaa                                                                                           >  1:155204/1‑150 (MQ=255)
                                                        gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAAct                                                                                         <  2:1483953/150‑1 (MQ=255)
                                                        gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAAct                                                                                         >  1:2758473/1‑150 (MQ=255)
                                                         cGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                        >  2:1217049/1‑150 (MQ=255)
                                                         cGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                        <  2:4974390/150‑1 (MQ=255)
                                                           aTTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActaac                                                                                      >  1:4516728/1‑147 (MQ=255)
                                                                tgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTtccttcct                                                                                 >  1:2882511/1‑150 (MQ=255)
                                                                 gcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTg                                                                                <  2:1719679/150‑1 (MQ=255)
                                                                  ccTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGa                                                                               <  1:126121/150‑1 (MQ=255)
                                                                    tctTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAac                                                                             >  1:3910279/1‑150 (MQ=255)
                                                                        cATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                                         >  2:922435/1‑150 (MQ=255)
                                                                         aTTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAt                                                                        <  2:308755/150‑1 (MQ=255)
                                                                            gtgGTAGTAAAACATTGGTGTTAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCt                                                                     <  2:2206621/150‑1 (MQ=255)
                                                                              tgaagaaAAACATTGGTGTAAACGACAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCttt                                                                   >  2:1863585/2‑150 (MQ=255)
                                                                               gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                                                  <  2:2120651/150‑1 (MQ=255)
                                                                               gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                                                  <  1:1883820/150‑1 (MQ=255)
                                                                                   aaaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAc                                                              <  1:3027817/150‑1 (MQ=255)
                                                                                    aaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACa                                                             <  2:2302288/150‑1 (MQ=255)
                                                                                      aaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                           >  2:4748903/1‑150 (MQ=255)
                                                                                      aaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                           <  1:4045853/150‑1 (MQ=255)
                                                                                       aCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                          >  2:4345968/1‑150 (MQ=255)
                                                                                        ccttGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTc                                                         <  2:5004998/148‑1 (MQ=255)
                                                                                           tGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGTTGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCATCGTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAt                                                      <  2:4652814/150‑1 (MQ=255)
                                                                                               gtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAAt                                                  >  1:2505578/1‑150 (MQ=255)
                                                                                                tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                                 <  1:3662498/150‑1 (MQ=255)
                                                                                                tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                                 <  2:2034405/150‑1 (MQ=255)
                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               >  1:2182763/1‑150 (MQ=255)
                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               <  2:423288/150‑1 (MQ=255)
                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               <  2:155204/150‑1 (MQ=255)
                                                                                                    tgTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgc                                             >  1:4760196/2‑150 (MQ=255)
                                                                                                    cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgc                                             >  1:3870084/1‑150 (MQ=255)
                                                                                                     gTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgcg                                            >  1:1950676/1‑150 (MQ=255)
                                                                                                       cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCa                                          <  2:1294207/150‑1 (MQ=255)
                                                                                                        aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAt                                         >  2:1744599/1‑150 (MQ=255)
                                                                                                        aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAt                                         <  1:1775199/150‑1 (MQ=255)
                                                                                                           cTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                                      <  1:4170444/150‑1 (MQ=255)
                                                                                                           cTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                                      <  2:4714524/150‑1 (MQ=255)
                                                                                                             ggTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                                    >  2:480810/1‑150 (MQ=255)
                                                                                                                     aGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCgg                            >  2:3435710/1‑150 (MQ=255)
                                                                                                                      ggAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAAAGCTCGATGAATACCGCGCATTATAAGAGCCCGGc                           >  2:1750542/1‑150 (MQ=255)
                                                                                                                          tGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCATGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCttt                       <  1:2950415/150‑1 (MQ=255)
                                                                                                                             aaaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAc                    >  2:756792/1‑150 (MQ=255)
                                                                                                                              aaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   <  1:476864/150‑1 (MQ=255)
                                                                                                                              aaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   >  2:669840/1‑150 (MQ=255)
                                                                                                                              aaaCGGTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCACCTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   <  2:4279300/150‑1 (MQ=255)
                                                                                                                               aaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAg                  <  2:4036435/150‑1 (MQ=255)
                                                                                                                                   ttCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCAtt              <  2:2932263/150‑1 (MQ=255)
                                                                                                                                    tCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTa             <  1:3951661/150‑1 (MQ=255)
                                                                                                                                     ccTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAt            >  2:1951087/1‑150 (MQ=255)
                                                                                                                                        cGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGc         >  2:555206/1‑150 (MQ=255)
                                                                                                                                          acacCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCgg       <  1:936715/150‑1 (MQ=255)
                                                                                                                                           cacCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTGCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGa      <  2:3944976/150‑1 (MQ=255)
                                                                                                                                             cccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaaa    >  1:5040491/1‑150 (MQ=255)
                                                                                                                                              ccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAAc   <  2:5015601/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG  >  CP009273/1296583‑1296875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑