Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,318,228 C→A 92.4% S251* (TCA→TAA)  yciV → PHP domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,318,2280CA92.4% 785.5 / 45.5 281S251* (TCA→TAA) yciVPHP domain protein
Reads supporting (aligned to +/- strand):  ref base C (8/14);  new base A (119/140);  total (127/154)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

ACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATAT  >  CP009273/1318081‑1318374
                                                                                                                                                   |                                                                                                                                                  
aCAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATcac                                                                                                                                                  <  1:1784262/150‑1 (MQ=255)
     cTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGa                                                                                                                                             <  2:850512/150‑1 (MQ=255)
     cTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGa                                                                                                                                             <  2:4283908/150‑1 (MQ=255)
       tAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCACTCGCCCTATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCAGCAGGTCTGGGCAGAACAAGGATc                                                                                                                                           >  2:4400446/1‑150 (MQ=255)
        aGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCt                                                                                                                                          >  2:4069118/1‑150 (MQ=255)
        aGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCt                                                                                                                                          <  1:4720517/150‑1 (MQ=255)
             tAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGAttt                                                                                                                                     >  1:1887259/1‑150 (MQ=255)
                aTGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTtca                                                                                                                                  <  1:2247917/150‑1 (MQ=255)
                 tGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTtcat                                                                                                                                 >  2:3205783/1‑150 (MQ=255)
                  ggCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTtcatc                                                                                                                                >  2:1870849/1‑150 (MQ=255)
                   gCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTtcatca                                                                                                                               >  2:4586284/1‑150 (MQ=255)
                      gAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGcc                                                                                                                            <  2:900492/150‑1 (MQ=255)
                        aaaGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCAt                                                                                                                          <  2:4851075/150‑1 (MQ=255)
                          aGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGc                                                                                                                        >  1:2943792/1‑150 (MQ=255)
                           gATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGcc                                                                                                                       >  1:1573949/1‑150 (MQ=255)
                           gATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGcc                                                                                                                       <  1:1001307/150‑1 (MQ=255)
                            aTTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGccc                                                                                                                      <  1:4564948/150‑1 (MQ=255)
                            aTTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGccc                                                                                                                      <  1:3237306/150‑1 (MQ=255)
                             ttGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCg                                                                                                                     <  1:998924/150‑1 (MQ=255)
                                 ttGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGa                                                                                                                 <  2:3922706/150‑1 (MQ=255)
                                  tGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGAt                                                                                                                >  1:875993/1‑150 (MQ=255)
                                   gcgcATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATc                                                                                                               >  1:2632684/1‑150 (MQ=255)
                                   gcgcATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATc                                                                                                               >  1:3553108/1‑150 (MQ=255)
                                    cgcATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCg                                                                                                              >  2:2904221/1‑150 (MQ=255)
                                       aTTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTCTGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAAc                                                                                                           >  2:1017494/1‑150 (MQ=255)
                                       aTTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAAc                                                                                                           >  1:72800/1‑150 (MQ=255)
                                       aTTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAAc                                                                                                           >  1:5015251/1‑150 (MQ=255)
                                       aTTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAAc                                                                                                           >  2:4321496/1‑150 (MQ=255)
                                        ttttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACt                                                                                                          >  1:2311843/1‑150 (MQ=255)
                                          ttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTgg                                                                                                        >  1:3589715/1‑150 (MQ=255)
                                           tGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTggg                                                                                                       >  1:4516551/1‑150 (MQ=255)
                                               gAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGt                                                                                                   >  1:4059861/1‑150 (MQ=255)
                                               gAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGt                                                                                                   >  2:4781728/1‑150 (MQ=255)
                                               gAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCCAACTGGGTCGt                                                                                                   >  2:3468355/1‑150 (MQ=255)
                                                aaCACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTa                                                                                                  >  1:3826400/1‑150 (MQ=255)
                                                    ccacGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTACAct                                                                                              >  2:2025845/1‑150 (MQ=255)
                                                    ccacGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAAct                                                                                              >  2:3852867/1‑150 (MQ=255)
                                                     cacGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAActc                                                                                             <  2:2142851/150‑1 (MQ=255)
                                                        ggTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGTCATGCCCGTGGATCGAACTGGGTCGTAAACTCTgg                                                                                          >  1:94088/1‑150 (MQ=255)
                                                        ggTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTgg                                                                                          >  2:559225/1‑150 (MQ=255)
                                                        ggTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTgg                                                                                          >  2:2698392/1‑150 (MQ=255)
                                                        ggTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTgg                                                                                          >  2:4267065/1‑150 (MQ=255)
                                                          tGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCt                                                                                        <  2:3182199/150‑1 (MQ=255)
                                                               cgATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCg                                                                                   <  1:2066923/150‑1 (MQ=255)
                                                                  tGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAg                                                                                <  1:4417165/150‑1 (MQ=255)
                                                                   ggAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAgg                                                                               <  2:2445228/150‑1 (MQ=255)
                                                                   ggAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAgg                                                                               >  1:462782/1‑150 (MQ=255)
                                                                   ggAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAgg                                                                               >  1:4247089/1‑150 (MQ=255)
                                                                    gAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGc                                                                              <  2:1278286/150‑1 (MQ=255)
                                                                         cgcgCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGa                                                                         >  2:1744284/1‑150 (MQ=255)
                                                                         cgcgCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGa                                                                         <  2:5015251/150‑1 (MQ=255)
                                                                           cgcAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAg                                                                       <  2:3396311/150‑1 (MQ=255)
                                                                              aGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCg                                                                    <  2:252369/150‑1 (MQ=255)
                                                                              aGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCg                                                                    >  2:3069863/1‑150 (MQ=255)
                                                                              aGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCg                                                                    >  2:3876469/1‑150 (MQ=255)
                                                                                  ccAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTg                                                                <  1:2904082/150‑1 (MQ=255)
                                                                                   cAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTgg                                                               >  1:351152/1‑150 (MQ=255)
                                                                                    aGCAATCGCCCAATGACCGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCAGGCCCGTGGATCGAACTGGGTCGGAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGc                                                              <  2:1505937/150‑1 (MQ=255)
                                                                                      cAATCGCCCAATGAAAGTACCCAGCTGTCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACCAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAg                                                            <  1:3926148/150‑1 (MQ=255)
                                                                                       aaTCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGc                                                           >  1:3719249/1‑150 (MQ=255)
                                                                                        aTCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCt                                                          <  1:3535365/150‑1 (MQ=255)
                                                                                        aTCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCt                                                          <  1:3808773/150‑1 (MQ=255)
                                                                                            cccAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATgg                                                      <  1:1726251/150‑1 (MQ=255)
                                                                                             ccAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATggg                                                     <  1:622680/150‑1 (MQ=255)
                                                                                                 tGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACa                                                 >  2:1284086/1‑150 (MQ=255)
                                                                                                  gAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAg                                                <  2:1854738/150‑1 (MQ=255)
                                                                                                     cGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCg                                             <  2:2943792/150‑1 (MQ=255)
                                                                                                      gTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGc                                            <  1:1213584/150‑1 (MQ=255)
                                                                                                        aCCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAg                                          <  1:3036958/150‑1 (MQ=255)
                                                                                                            aGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAaca                                      >  1:2393788/1‑150 (MQ=255)
                                                                                                            aGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAaca                                      <  1:365514/150‑1 (MQ=255)
                                                                                                              cTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAAcacc                                    >  2:2870383/1‑150 (MQ=255)
                                                                                                              cTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAAcacc                                    <  1:2416473/150‑1 (MQ=255)
                                                                                                               tGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAAcacca                                   <  2:1690067/150‑1 (MQ=255)
                                                                                                                 gcggcCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACcaca                                 <  2:3754880/150‑1 (MQ=255)
                                                                                                                   ggcCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACaga                               >  2:2552271/1‑150 (MQ=255)
                                                                                                                   ggcCCTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACaga                               <  2:751815/150‑1 (MQ=255)
                                                                                                                     cccTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACagaga                             >  1:4984271/1‑150 (MQ=255)
                                                                                                                      ccTTGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACagagag                            >  1:1053482/1‑150 (MQ=255)
                                                                                                                        ttGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTTATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAggg                          <  2:1590080/150‑1 (MQ=255)
                                                                                                                        ttGCGCGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAggg                          >  1:1082624/1‑150 (MQ=255)
                                                                                                                          gcgcGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGaa                        <  2:565697/150‑1 (MQ=255)
                                                                                                                          gcgcGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGaa                        <  2:1469367/150‑1 (MQ=255)
                                                                                                                          gcgcGTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGaa                        <  1:485258/150‑1 (MQ=255)
                                                                                                                           cgcgTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAAt                       >  1:5090417/1‑150 (MQ=255)
                                                                                                                            gcgTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAAtt                      <  2:4615226/150‑1 (MQ=255)
                                                                                                                             cgTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTa                     <  1:3116035/150‑1 (MQ=255)
                                                                                                                              gTCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTAt                    >  2:4171847/1‑150 (MQ=255)
                                                                                                                               tCAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATg                   <  2:941982/150‑1 (MQ=255)
                                                                                                                                cAGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGa                  >  1:2508280/1‑150 (MQ=255)
                                                                                                                                 aGCATCATTTATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAg                 <  2:540035/150‑1 (MQ=255)
                                                                                                                                       atTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCACAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtt           >  2:1342595/1‑150 (MQ=255)
                                                                                                                                         ttATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtttt         <  2:665650/150‑1 (MQ=255)
                                                                                                                                         ttATGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtttt         <  1:1458085/150‑1 (MQ=255)
                                                                                                                                         tgaTGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtttt         <  2:1959897/148‑1 (MQ=255)
                                                                                                                                          tATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGttttt        <  2:609474/150‑1 (MQ=255)
                                                                                                                                           aTGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtttttt       >  1:2702328/1‑150 (MQ=255)
                                                                                                                                            tGGGCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGttttttt      <  2:3525758/150‑1 (MQ=255)
                                                                                                                                              ggCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTtat    <  1:2072262/150‑1 (MQ=255)
                                                                                                                                               gCATAACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTtata   <  2:4015231/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATAT  >  CP009273/1318081‑1318374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑