Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,328,313 C→A 57.8% R68S (CGT→AGT)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,328,3130CA57.8% 38.6 / inf 223R68S (CGT→AGT) cysBN‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (46/48);  new base A (70/59);  total (116/107)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 224 total aligned reads displayed.

TCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAG  >  CP009273/1328167‑1328462
                                                                                                                                                  |                                                                                                                                                     
tctcATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTa                                                                                                                                                    >  1:2751759/1‑150 (MQ=255)
      caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgctc                                                                                                                                              <  1:3973466/150‑1 (MQ=255)
      caACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcgctc                                                                                                                                              >  2:5122465/1‑150 (MQ=255)
       aaCAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcgctcg                                                                                                                                             <  1:1294187/150‑1 (MQ=255)
       aaCAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTAtcgctcg                                                                                                                                             >  1:2735682/1‑150 (MQ=255)
         cAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTcgcg                                                                                                                                           >  2:4646194/1‑150 (MQ=255)
             ggAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGt                                                                                                                                       >  2:2154843/1‑150 (MQ=255)
             ggAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGt                                                                                                                                       <  2:1324902/150‑1 (MQ=255)
             ggAAGGACTTTACACATCACAACCCGGGAACAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACTCCAGCAGGGCACGAAATAATTATTATCGCTCGCGaatt                                                                                                                                       >  1:2906276/1‑148 (MQ=255)
              gAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTc                                                                                                                                      >  1:1496190/1‑150 (MQ=255)
                aGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCt                                                                                                                                    <  1:4505547/150‑1 (MQ=255)
                  gACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGt                                                                                                                                  <  2:1112899/150‑1 (MQ=255)
                  gACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGt                                                                                                                                  <  2:1987561/150‑1 (MQ=255)
                  gACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGt                                                                                                                                  >  2:185739/1‑150 (MQ=255)
                     tttACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGa                                                                                                                               >  1:2205024/1‑150 (MQ=255)
                      ttACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGaa                                                                                                                              >  2:288821/1‑150 (MQ=255)
                       tACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGaaa                                                                                                                             <  2:4636432/150‑1 (MQ=255)
                        acacATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAg                                                                                                                            >  2:2475814/1‑150 (MQ=255)
                        acacATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCAGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAg                                                                                                                            >  2:5015071/1‑150 (MQ=255)
                            aTCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGa                                                                                                                        >  2:3355709/1‑150 (MQ=255)
                             tCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGAt                                                                                                                       >  1:882439/1‑150 (MQ=255)
                                      gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGNTAGGCATTCAAATTTATTCCAGAAGCGTCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTATTATCGCTCGCGAAGTCCAGTCGAAAGTCGATGCCATaaaa                                                                                                              >  1:2680778/1‑150 (MQ=255)
                                      gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATaaaa                                                                                                              >  2:4159617/1‑150 (MQ=255)
                                      gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATaaaa                                                                                                              >  1:4116723/1‑150 (MQ=255)
                                       ggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAAt                                                                                                             >  1:2545719/1‑150 (MQ=255)
                                       ggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAAt                                                                                                             <  2:3830129/150‑1 (MQ=255)
                                        gATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc                                                                                                            >  1:3741457/1‑150 (MQ=255)
                                        gATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc                                                                                                            <  2:2072907/150‑1 (MQ=255)
                                            aGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGtt                                                                                                        <  2:109126/150‑1 (MQ=255)
                                              tAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGAAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGACATAAAATCGGTTGc                                                                                                      >  2:1374490/1‑150 (MQ=255)
                                               aaaCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGcc                                                                                                     >  1:2117314/1‑150 (MQ=255)
                                               aaaCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGATGcc                                                                                                     >  1:1232353/1‑150 (MQ=255)
                                                 aCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCgg                                                                                                   <  1:4684364/150‑1 (MQ=255)
                                                   aaGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGgag                                                                                                 >  1:5059969/1‑150 (MQ=255)
                                                   aaGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGgag                                                                                                 >  2:4328504/1‑150 (MQ=255)
                                                    aGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGgaga                                                                                                >  1:831213/1‑150 (MQ=255)
                                                     gTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTGTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGagag                                                                                               <  2:2209595/150‑1 (MQ=255)
                                                       cAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca                                                                                             >  2:756669/1‑150 (MQ=255)
                                                        aGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGcac                                                                                            <  2:4962762/150‑1 (MQ=255)
                                                          aaTGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCacac                                                                                          <  2:901249/150‑1 (MQ=255)
                                                            tGCTGGAAGCCGAGCTAGGCATTCAAATTTTGTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCt                                                                                        <  2:1750982/150‑1 (MQ=255)
                                                             gCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTg                                                                                       <  1:78712/150‑1 (MQ=255)
                                                              cTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTgt                                                                                      <  1:1068975/150‑2 (MQ=255)
                                                               tGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGc                                                                                     >  2:1074138/1‑150 (MQ=255)
                                                               tGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGc                                                                                     >  1:3312231/1‑150 (MQ=255)
                                                                ggAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGcc                                                                                    >  1:459731/1‑150 (MQ=255)
                                                                ggAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGcc                                                                                    >  1:617806/1‑150 (MQ=255)
                                                                 gAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCg                                                                                   <  1:1806007/150‑1 (MQ=255)
                                                                  aaGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCgg                                                                                  <  1:524862/150‑1 (MQ=255)
                                                                   aGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGa                                                                                 >  2:4476911/1‑150 (MQ=255)
                                                                   aGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGa                                                                                 >  2:2991588/1‑150 (MQ=255)
                                                                       gAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATaaa                                                                             >  1:2430291/1‑150 (MQ=255)
                                                                        aGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAg                                                                            >  2:4899700/1‑150 (MQ=255)
                                                                         gCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACCCCTGGCCGGATACAgg                                                                           >  2:153022/1‑150 (MQ=255)
                                                                          cTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCCCACCTGGCCGGagcagggt                                                                          >  2:3024118/1‑143 (MQ=255)
                                                                          cTAGGCATTAAAAATTTTTACCGAAGCGGCAAGCACCAGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATAGCTCGCGAAGTCCTGTCGAAAGTCGATGCCCTAAAATCGGTTGCCGGAGAGCCCACCTGGCCGGATAAAGGt                                                                          >  2:1457353/1‑150 (MQ=255)
                                                                           tAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGtt                                                                         >  1:3908232/1‑150 (MQ=255)
                                                                            aGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTc                                                                        <  2:3384529/150‑1 (MQ=255)
                                                                             ggCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCa                                                                       >  2:206947/1‑150 (MQ=255)
                                                                             ggCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCa                                                                       <  2:3207448/150‑1 (MQ=255)
                                                                              gCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCAc                                                                      >  1:3005552/1‑150 (MQ=255)
                                                                               cATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACt                                                                     >  2:4434682/1‑150 (MQ=255)
                                                                                    aaaTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGtata                                                                >  1:4293105/1‑150 (MQ=255)
                                                                                    aaaTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGtata                                                                <  1:1108590/150‑1 (MQ=255)
                                                                                      aTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATc                                                              >  2:4540499/1‑150 (MQ=255)
                                                                                      aTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATc                                                              <  1:731841/150‑1 (MQ=255)
                                                                                             cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGccacca                                                       >  2:2756463/1‑150 (MQ=255)
                                                                                              ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCcaccac                                                      <  1:3505394/150‑1 (MQ=255)
                                                                                              ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCcaccac                                                      >  1:2488428/1‑150 (MQ=255)
                                                                                              ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCcaccac                                                      <  1:3407134/150‑1 (MQ=255)
                                                                                                 aaGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCa                                                   >  1:3115016/1‑150 (MQ=255)
                                                                                                  aGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                  >  2:862457/1‑150 (MQ=255)
                                                                                                  aGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                  <  2:3311546/150‑1 (MQ=255)
                                                                                                   gCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATa                                                 <  1:3235946/150‑1 (MQ=255)
                                                                                                   gCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATa                                                 <  1:3411689/150‑1 (MQ=255)
                                                                                                        aaGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAg                                            >  1:4975162/1‑150 (MQ=255)
                                                                                                        aaGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAg                                            >  2:1963456/1‑150 (MQ=255)
                                                                                                        aaGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAg                                            >  2:4099120/1‑150 (MQ=255)
                                                                                                            aCCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCAc                                        <  1:1353120/150‑1 (MQ=255)
                                                                                                               tGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCt                                     <  2:3492264/150‑1 (MQ=255)
                                                                                                               tGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTACACTGTATCTCGCCACCACGCATACCCAGGCACGCt                                     >  1:281638/1‑150 (MQ=255)
                                                                                                                gACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTa                                    <  1:1155277/150‑1 (MQ=255)
                                                                                                                 aCGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTAc                                   >  1:1206722/1‑150 (MQ=255)
                                                                                                                  cGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACg                                  >  2:4246450/1‑150 (MQ=255)
                                                                                                                  cGCAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACg                                  >  2:2560762/1‑150 (MQ=255)
                                                                                                                    cAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                                <  2:2735682/150‑1 (MQ=255)
                                                                                                                    cAGGTAACGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                                <  2:1678290/150‑1 (MQ=255)
                                                                                                                        tAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTAc                            >  2:3044987/1‑150 (MQ=255)
                                                                                                                         aaCGCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTAcc                           >  1:1741930/1‑150 (MQ=255)
                                                                                                                            gCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCaaa                        >  2:4210272/1‑150 (MQ=255)
                                                                                                                            gCCAGCAGGGCAAGAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCaaa                        >  2:720686/1‑150 (MQ=255)
                                                                                                                              cagcagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACg                      <  1:1008448/150‑1 (MQ=255)
                                                                                                                              cagcagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACg                      <  1:1008394/150‑1 (MQ=255)
                                                                                                                                gcagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGtc                    <  1:4712805/150‑1 (MQ=255)
                                                                                                                                   gggCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGtcatc                 <  2:2205024/150‑1 (MQ=255)
                                                                                                                                         gAAATAATTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGc           <  2:3188537/150‑1 (MQ=255)
                                                                                                                                           aataatTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCtt         <  1:1995131/150‑1 (MQ=255)
                                                                                                                                            ataatTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCttt        <  2:1862100/150‑1 (MQ=255)
                                                                                                                                               atTAGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACTTCATCAAAGGCTTTAtt     >  2:3721314/1‑150 (MQ=255)
                                                                                                                                                  cGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAg  >  1:960307/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAG  >  CP009273/1328167‑1328462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑