Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 103,467 G→T 100% D142Y (GAT→TAT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273103,4670GT99.6% 912.7 / ‑4.4 277D142Y (GAT→TAT) lpxCUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (139/136);  total (140/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

TTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTGCGC  >  CP009273/103321‑103611
                                                                                                                                                  |                                                                                                                                                
 tATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATaa                                                                                                                                              <  2:3925728/150‑1 (MQ=255)
 tATCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATaa                                                                                                                                              >  1:2649786/1‑150 (MQ=255)
   tCGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGt                                                                                                                                            >  2:1654774/1‑150 (MQ=255)
    cGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTCTAAGTg                                                                                                                                           >  2:2941843/1‑150 (MQ=255)
    cGAAGTTAACGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTg                                                                                                                                           <  1:4127182/150‑1 (MQ=255)
       aGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGc                                                                                                                                        >  1:3816303/1‑150 (MQ=255)
            aCGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAt                                                                                                                                   <  2:2721245/150‑1 (MQ=255)
            aCGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAt                                                                                                                                   <  2:3936137/150‑1 (MQ=255)
            aCGCGCCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAt                                                                                                                                   >  1:3277446/1‑150 (MQ=255)
             cgcgCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAtt                                                                                                                                  <  2:3171438/150‑1 (MQ=255)
               cgcCGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTa                                                                                                                                <  1:2945656/150‑1 (MQ=255)
                 ccGGAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAg                                                                                                                              <  2:1494546/150‑1 (MQ=255)
                   ggAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc                                                                                                                            <  2:4934614/150‑1 (MQ=255)
                   ggAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc                                                                                                                            <  2:2455768/150‑1 (MQ=255)
                    gAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg                                                                                                                           <  1:1044536/150‑1 (MQ=255)
                    gAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg                                                                                                                           <  2:3608600/150‑1 (MQ=255)
                    gAAATCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg                                                                                                                           <  1:2985640/150‑1 (MQ=255)
                       aTCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTAc                                                                                                                        >  1:688798/1‑150 (MQ=255)
                       aTCCCGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTAc                                                                                                                        <  2:1969784/150‑1 (MQ=255)
                          ccGATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAAt                                                                                                                     <  1:5083418/150‑1 (MQ=255)
                            gATAATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATgg                                                                                                                   >  2:2176761/1‑150 (MQ=255)
                             ataaTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGt                                                                                                                  <  2:2565270/147‑1 (MQ=255)
                               cATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGttt                                                                                                                <  1:2684030/150‑1 (MQ=255)
                               aaTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGttt                                                                                                                >  1:1399603/2‑150 (MQ=255)
                               aaTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAGTTAAGCCGTACAATGGttt                                                                                                                >  1:3977910/2‑150 (MQ=255)
                                aTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtttt                                                                                                               <  1:284691/150‑1 (MQ=255)
                                aTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtttt                                                                                                               <  1:201769/150‑1 (MQ=255)
                                     cGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCt                                                                                                          <  1:3520442/150‑1 (MQ=255)
                                         aGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAt                                                                                                      >  2:1231843/1‑150 (MQ=255)
                                          gcgcCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGTGGAGTTGAACTGCGCCAATAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGATGAATTTAAGCCGTACAATGGTTTTTCGCTGGAtt                                                                                                     <  1:808857/150‑1 (MQ=255)
                                          gcgcCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAtt                                                                                                     <  2:3293725/150‑1 (MQ=255)
                                           cgccgcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAttt                                                                                                    >  2:3394052/1‑150 (MQ=255)
                                           cgccgcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAttt                                                                                                    >  1:2607359/1‑150 (MQ=255)
                                             ccgcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCa                                                                                                  <  2:3893152/150‑1 (MQ=255)
                                               gcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCAcc                                                                                                >  1:1560040/1‑150 (MQ=255)
                                                cTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCa                                                                                               >  2:1915739/1‑150 (MQ=255)
                                                  ccGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATc                                                                                             <  1:4347856/150‑1 (MQ=255)
                                                  ccGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATc                                                                                             <  1:2755011/150‑1 (MQ=255)
                                                      ttGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGAtt                                                                                         <  2:431471/150‑1 (MQ=255)
                                                       tGTATACCTGCTTCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGAttt                                                                                        <  1:3345680/150‑1 (MQ=255)
                                                         tataCCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTa                                                                                      >  2:4163394/1‑150 (MQ=255)
                                                           taCCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAACATTTGTGCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTAGAAGTGGGCTGAATTTAAGCCGTACAATGGATTTTCGCTGGAGTTCACCATCGATTTTAAc                                                                                    <  2:2835458/150‑1 (MQ=255)
                                                             ccTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCa                                                                                  <  2:1718209/150‑1 (MQ=255)
                                                                  tgctTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCgg                                                                             >  2:1147974/1‑150 (MQ=255)
                                                                    ctTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGGCTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTGTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCt                                                                           >  2:4351141/1‑150 (MQ=255)
                                                                    ctTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCt                                                                           <  2:1602532/150‑1 (MQ=255)
                                                                      tGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTAt                                                                         <  2:4213865/150‑1 (MQ=255)
                                                                       gACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTAtt                                                                        >  2:5115499/1‑150 (MQ=255)
                                                                       gACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTAtt                                                                        <  2:605583/150‑1 (MQ=255)
                                                                       gACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTAtt                                                                        <  2:1877860/150‑1 (MQ=255)
                                                                          gCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGAt                                                                     <  2:4999692/150‑1 (MQ=255)
                                                                                aTCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGc                                                               >  1:1018433/1‑150 (MQ=255)
                                                                                 tCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCa                                                              <  1:1142629/150‑1 (MQ=255)
                                                                                  cgacgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCaa                                                             >  1:1275885/1‑150 (MQ=255)
                                                                                  cgacgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCaa                                                             >  1:1922414/1‑150 (MQ=255)
                                                                                   gacgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAAc                                                            >  1:379667/1‑150 (MQ=255)
                                                                                     cgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCa                                                          <  2:3049755/150‑1 (MQ=255)
                                                                                      gaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAg                                                         >  2:1554403/1‑150 (MQ=255)
                                                                                       aGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgc                                                        >  2:3721575/1‑150 (MQ=255)
                                                                                         ttGAACTGCGCCAACAAATTTGTTCGCTTCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgcgc                                                      <  2:4124937/150‑1 (MQ=255)
                                                                                         ttGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgcgc                                                      <  2:1003875/150‑1 (MQ=255)
                                                                                          tGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCt                                                     <  2:1068697/150‑1 (MQ=255)
                                                                                             aCTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATg                                                  <  1:2508040/150‑1 (MQ=255)
                                                                                               tGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCg                                                >  2:2328581/1‑150 (MQ=255)
                                                                                                gcgcCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACAATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGa                                               >  1:478634/1‑150 (MQ=255)
                                                                                                 cgcCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGAt                                              >  2:3371394/1‑150 (MQ=255)
                                                                                                  gcCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATg                                             >  1:389072/1‑150 (MQ=255)
                                                                                                    cAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGaa                                           >  1:2211244/1‑150 (MQ=255)
                                                                                                         aaTTTGTTCGCATCATAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTtct                                      <  2:751933/150‑1 (MQ=255)
                                                                                                            ttGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCg                                   <  2:2982819/150‑1 (MQ=255)
                                                                                                              gTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCt                                 <  2:1220319/150‑1 (MQ=255)
                                                                                                              gTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCt                                 >  1:2058176/1‑150 (MQ=255)
                                                                                                               ttCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTg                                <  1:4448548/150‑1 (MQ=255)
                                                                                                               ttCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTg                                <  2:1284215/150‑1 (MQ=255)
                                                                                                                 cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              <  1:72942/150‑1 (MQ=255)
                                                                                                                 cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              <  2:1090457/150‑1 (MQ=255)
                                                                                                                 cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              >  2:4232502/1‑150 (MQ=255)
                                                                                                                 cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              <  2:4065438/150‑1 (MQ=255)
                                                                                                                 cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCAATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              <  2:3019866/150‑1 (MQ=255)
                                                                                                                   cATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGc                            >  2:1874178/1‑150 (MQ=255)
                                                                                                                   cATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGc                            >  2:3999386/1‑150 (MQ=255)
                                                                                                                    aTCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCg                           >  2:4590651/1‑150 (MQ=255)
                                                                                                                    aTCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTATAACCATCCGGCTATTGATTACAGCAACAAGCGCTATGCGATGAACTTCTCCGCTGATGCg                           >  2:2677476/1‑150 (MQ=255)
                                                                                                                       aaaGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGttt                        >  1:3139195/1‑150 (MQ=255)
                                                                                                                        aaGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTa                       >  1:4213664/1‑150 (MQ=255)
                                                                                                                         agagACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTAt                      >  1:3635869/1‑150 (MQ=255)
                                                                                                                         agagACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTAt                      >  1:2742100/1‑150 (MQ=255)
                                                                                                                            gaCTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATgcg                   >  1:2455416/1‑150 (MQ=255)
                                                                                                                               tGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCa                >  2:4656721/1‑150 (MQ=255)
                                                                                                                               tGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATGTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCa                <  2:4811492/150‑1 (MQ=255)
                                                                                                                                   cGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATc            <  1:4723269/150‑1 (MQ=255)
                                                                                                                                    gtgtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCa           <  1:4862100/150‑1 (MQ=255)
                                                                                                                                     tgtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAg          >  2:501128/1‑150 (MQ=255)
                                                                                                                                      gtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGc         <  2:2649786/150‑1 (MQ=255)
                                                                                                                                          aaGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTg     >  2:3524158/1‑150 (MQ=255)
                                                                                                                                          aaGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTg     >  2:3702206/1‑150 (MQ=255)
                                                                                                                                          aaGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTg     >  1:3429212/1‑150 (MQ=255)
                                                                                                                                          aaGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTg     >  1:1810508/1‑150 (MQ=255)
                                                                                                                                           aGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTgc    <  2:4864369/150‑1 (MQ=255)
                                                                                                                                            gATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTgcg   >  1:1475290/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTGCGC  >  CP009273/103321‑103611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑