Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,454,724 G→T 100% G472C (GGT→TGT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,454,7240GT100.0% 1118.7 / NA 336G472C (GGT→TGT) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (168/168);  total (168/168)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 336 total aligned reads displayed.

GTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCA  >  CP009273/1454577‑1454870
                                                                                                                                                   |                                                                                                                                                  
    aTTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATg                                                                                                                                              <  2:2159162/150‑1 (MQ=255)
      tATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAg                                                                                                                                            <  1:551411/150‑1 (MQ=255)
          cATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCAt                                                                                                                                        <  1:5114999/150‑1 (MQ=255)
           atatGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATa                                                                                                                                       <  2:2644437/150‑1 (MQ=255)
             atGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAg                                                                                                                                     >  1:3730742/1‑150 (MQ=255)
              tGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAgg                                                                                                                                    >  1:4082152/1‑150 (MQ=255)
               ggCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGc                                                                                                                                   >  2:2324473/1‑150 (MQ=255)
                  ccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTg                                                                                                                                <  1:111309/150‑1 (MQ=255)
                   cACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTgg                                                                                                                               >  1:2877178/1‑150 (MQ=255)
                    aCTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGc                                                                                                                              <  1:4849233/150‑1 (MQ=255)
                    aCTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTCCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAATGCCTGGc                                                                                                                              >  1:2665769/1‑150 (MQ=255)
                        gCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaaa                                                                                                                          <  1:3775343/150‑1 (MQ=255)
                          cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATg                                                                                                                        <  1:1434241/150‑1 (MQ=255)
                          cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATg                                                                                                                        >  2:498226/1‑150 (MQ=255)
                           tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGc                                                                                                                       >  2:4248115/1‑150 (MQ=255)
                           tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGc                                                                                                                       >  2:3477420/1‑150 (MQ=255)
                             gggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                                                                     >  1:4789516/1‑150 (MQ=255)
                             gggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                                                                     <  2:552325/150‑1 (MQ=255)
                                aGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCTACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                                                                                                  <  1:3510896/150‑1 (MQ=255)
                                aGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                                                                                                  >  2:5037806/1‑150 (MQ=255)
                                aGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                                                                                                  >  2:269352/1‑150 (MQ=255)
                                  cgcAACTTGGCTGGCAGCGAATATTAGGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                                >  2:4833753/1‑150 (MQ=255)
                                  cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                                <  2:246366/150‑1 (MQ=255)
                                     aaCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATg                                                                                                             >  1:2559308/1‑150 (MQ=255)
                                      aCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                                                                                                            <  2:4027509/150‑1 (MQ=255)
                                       cTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                                                                                                           <  1:1002228/150‑1 (MQ=255)
                                        ttGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAt                                                                                                          <  1:1352052/150‑1 (MQ=255)
                                           gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                                                                                                       <  1:4175286/150‑1 (MQ=255)
                                             tggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGaa                                                                                                     >  1:3317543/1‑150 (MQ=255)
                                               gcAGCGTATATTATTGATACTTGATAATCTACAGCATCACTATGGCGAATAACGCTATCTCACATGTTCATTGCTGCGCGTACGGGAGCTTCTGGAGAGCTTTTATGAGTCATAAGTCCTGGCAAAATGCCCATGAAATTTATGAGAAct                                                                                                   <  2:3898048/150‑2 (MQ=255)
                                                cAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGa                                                                                                  <  2:1905013/150‑1 (MQ=255)
                                                    gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc                                                                                              <  1:934307/150‑1 (MQ=255)
                                                     aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCt                                                                                             <  1:4459946/150‑1 (MQ=255)
                                                       tatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc                                                                                           >  2:1301400/1‑150 (MQ=255)
                                                        atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg                                                                                          <  1:4965189/150‑1 (MQ=255)
                                                        atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg                                                                                          >  2:846084/1‑150 (MQ=255)
                                                        atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg                                                                                          >  1:4105406/1‑150 (MQ=255)
                                                           aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                       >  2:445464/1‑150 (MQ=255)
                                                            aGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaa                                                                                      <  2:4634805/150‑1 (MQ=255)
                                                             ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTTCGCCAAAGGTCGCTTATGGAGAGCTGTTATGAGTCATAAGGCCAGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTTCGCCaaa                                                                                     >  1:569316/1‑150 (MQ=255)
                                                             ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaaa                                                                                     <  1:1669732/150‑1 (MQ=255)
                                                              gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGATATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg                                                                                    >  1:60669/1‑150 (MQ=255)
                                                               cTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgc                                                                                   >  2:1705424/1‑150 (MQ=255)
                                                               cTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgc                                                                                   >  2:3338379/1‑150 (MQ=255)
                                                               cTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgc                                                                                   <  1:2420504/150‑1 (MQ=255)
                                                                tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcg                                                                                  <  2:3373669/150‑1 (MQ=255)
                                                                  cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCt                                                                                <  2:2932948/150‑1 (MQ=255)
                                                                    tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTg                                                                              <  2:4089361/150‑1 (MQ=255)
                                                                    tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTg                                                                              <  2:4813425/150‑1 (MQ=255)
                                                                      aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGc                                                                            <  1:85025/150‑1 (MQ=255)
                                                                      aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCAGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGc                                                                            >  2:3049576/1‑150 (MQ=255)
                                                                        aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCAt                                                                          >  2:3158173/1‑150 (MQ=255)
                                                                           cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGa                                                                       <  2:755702/150‑1 (MQ=255)
                                                                           cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGa                                                                       >  2:3998702/1‑150 (MQ=255)
                                                                             aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACa                                                                     <  2:5024036/150‑1 (MQ=255)
                                                                                    cACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAtctc                                                              >  1:5092377/1‑150 (MQ=255)
                                                                                    cACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAtctc                                                              >  1:980610/1‑150 (MQ=255)
                                                                                     aCTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                                             >  2:1147190/1‑150 (MQ=255)
                                                                                      cTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCaa                                                            >  1:4367788/1‑150 (MQ=255)
                                                                                         tGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATgg                                                         >  1:1927140/1‑150 (MQ=255)
                                                                                            cGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATg                                                      >  2:570169/1‑150 (MQ=255)
                                                                                             gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGa                                                     >  1:3662412/1‑150 (MQ=255)
                                                                                             gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGa                                                     >  1:1932502/1‑150 (MQ=255)
                                                                                              aagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGaa                                                    >  1:1198617/1‑150 (MQ=255)
                                                                                              aagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGaa                                                    >  1:3124274/1‑150 (MQ=255)
                                                                                                taaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAgg                                                  >  2:193098/2‑150 (MQ=255)
                                                                                                 aaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                                 >  2:1417143/1‑150 (MQ=255)
                                                                                                 aaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                                 <  2:2071516/150‑1 (MQ=255)
                                                                                                    gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTTGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCttt                                              >  1:2980162/1‑150 (MQ=255)
                                                                                                     cTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTg                                             <  2:1077708/150‑1 (MQ=255)
                                                                                                      tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                                            <  2:2910728/150‑1 (MQ=255)
                                                                                                      tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                                            <  1:122920/150‑1 (MQ=255)
                                                                                                       aTCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCt                                           >  1:2017452/1‑150 (MQ=255)
                                                                                                         cGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt                                         <  1:3926643/150‑1 (MQ=255)
                                                                                                            ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                      <  1:5037805/150‑1 (MQ=255)
                                                                                                             cNTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCGTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTg                                     >  1:441003/1‑150 (MQ=255)
                                                                                                             cATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTg                                     <  1:2315046/150‑1 (MQ=255)
                                                                                                              aTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGa                                    <  2:1620954/150‑1 (MQ=255)
                                                                                                                gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                  >  2:435024/1‑150 (MQ=255)
                                                                                                                gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                  >  1:4621253/1‑150 (MQ=255)
                                                                                                                  tCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCAt                                <  1:3377704/150‑1 (MQ=255)
                                                                                                                      tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCACAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc                            <  2:4750105/150‑1 (MQ=255)
                                                                                                                      tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc                            <  1:2770073/150‑1 (MQ=255)
                                                                                                                       gctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCg                           <  1:1045499/150‑1 (MQ=255)
                                                                                                                       gctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCg                           >  1:1310711/1‑150 (MQ=255)
                                                                                                                       gctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCg                           >  2:1162925/1‑150 (MQ=255)
                                                                                                                         tgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTc                         <  2:2877178/150‑1 (MQ=255)
                                                                                                                          gcgcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCa                        >  2:3559728/1‑150 (MQ=255)
                                                                                                                            gcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt                      >  2:3874190/1‑150 (MQ=255)
                                                                                                                            gcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt                      >  2:25729/1‑150 (MQ=255)
                                                                                                                             ccAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTg                     >  1:3837277/1‑150 (MQ=255)
                                                                                                                                       cTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt           >  2:3761180/1‑150 (MQ=255)
                                                                                                                                         tCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTa         >  1:754796/1‑150 (MQ=255)
                                                                                                                                           tGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAAc       <  1:4680504/150‑1 (MQ=255)
                                                                                                                                             gagaGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACgg     >  2:3956138/1‑150 (MQ=255)
                                                                                                                                             gagaGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACgg     >  2:1323355/1‑150 (MQ=255)
                                                                                                                                              agagCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGt    <  2:1336034/150‑1 (MQ=255)
                                                                                                                                                agCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtca  <  1:2094623/150‑1 (MQ=255)
                                                                                                                                                agCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtca  <  2:2219058/150‑1 (MQ=255)
                                                                                                                                                agCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtca  >  2:665038/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCA  >  CP009273/1454577‑1454870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑