Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,469,880 A→T 100% I160I (ATA→ATT)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,469,8800AT77.3% 555.7 / 173.7 269I160I (ATA→ATT) ydbDPF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base T (114/94);  total (145/124)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 272 total aligned reads displayed.

TATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTA  >  CP009273/1469733‑1470025
                                                                                                                                                   |                                                                                                                                                 
tATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGAtttt                                                                                                                                                 >  2:1686746/1‑150 (MQ=255)
 aTTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATAttt                                                                                                                                                <  2:3649490/150‑1 (MQ=255)
  ttCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTa                                                                                                                                               <  2:1462684/150‑1 (MQ=255)
   tctcGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTAt                                                                                                                                              <  1:818029/150‑1 (MQ=255)
        gTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGAAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACTATTAAGGGGAGTATTAATGAGCGTGGGATTTTTATAAATg                                                                                                                                         >  1:4168869/1‑150 (MQ=255)
         tAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGa                                                                                                                                        >  1:2060933/1‑150 (MQ=255)
          aaCGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGAc                                                                                                                                       >  1:4318637/1‑150 (MQ=255)
            cGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACAt                                                                                                                                     <  2:1959042/150‑1 (MQ=255)
                  ggCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGc                                                                                                                               >  2:4498595/1‑150 (MQ=255)
                   gCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCa                                                                                                                              <  2:347350/150‑1 (MQ=255)
                   gCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCa                                                                                                                              >  2:309737/1‑150 (MQ=255)
                    cAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCAc                                                                                                                             <  2:2616708/150‑1 (MQ=255)
                     aaCGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCAcc                                                                                                                            >  1:1602691/1‑150 (MQ=255)
                     aaCGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCAcc                                                                                                                            <  1:2401338/150‑1 (MQ=255)
                     aaCGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCAcc                                                                                                                            <  1:1426082/150‑1 (MQ=255)
                      aCGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCt                                                                                                                           >  1:4036156/1‑150 (MQ=255)
                       cGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCtg                                                                                                                          <  2:3301689/150‑1 (MQ=255)
                        gAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCtgt                                                                                                                         >  2:3907425/1‑150 (MQ=255)
                          atatAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCtgttg                                                                                                                       <  2:2378618/150‑1 (MQ=255)
                           tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCtgttgt                                                                                                                      >  1:347345/1‑150 (MQ=255)
                           tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCtgttgt                                                                                                                      >  1:2314348/1‑150 (MQ=255)
                            ataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTgttgtt                                                                                                                     <  2:96143/150‑1 (MQ=255)
                             taGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTATGGGGAGTATTAATGCGCGTGGGATTTTTATCAAGGACATAATTGCACGGGttgttg                                                                                                                    >  2:5133171/1‑150 (MQ=255)
                             taGTTGCGGATTCACCACTGTTAATGACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGttgttg                                                                                                                    <  1:3187505/150‑1 (MQ=255)
                               ggtGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCg                                                                                                                  <  1:4338922/148‑1 (MQ=255)
                                   cGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCtt                                                                                                              >  1:4770863/1‑150 (MQ=255)
                                    ggATTCACCACTGGTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTc                                                                                                             <  2:2634785/150‑1 (MQ=255)
                                      aTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGa                                                                                                           <  1:4460776/150‑1 (MQ=255)
                                              cTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGt                                                                                                   <  2:224571/150‑1 (MQ=255)
                                                  tAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAAt                                                                                               <  2:3351608/150‑1 (MQ=255)
                                                    aTTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTc                                                                                             >  2:488905/1‑150 (MQ=255)
                                                     ttACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCa                                                                                            >  2:3740812/1‑150 (MQ=255)
                                                      tACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCAt                                                                                           >  1:4354745/1‑150 (MQ=255)
                                                       aCCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATg                                                                                          >  1:3441473/1‑150 (MQ=255)
                                                       aCCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATg                                                                                          >  1:2027755/1‑150 (MQ=255)
                                                         cGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGt                                                                                        >  2:3552533/1‑150 (MQ=255)
                                                         cGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGt                                                                                        <  2:1602550/150‑1 (MQ=255)
                                                         cGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGt                                                                                        >  2:4288969/1‑150 (MQ=255)
                                                          ggTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTc                                                                                       <  2:4065906/150‑1 (MQ=255)
                                                          ggTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTc                                                                                       >  2:2880084/1‑150 (MQ=255)
                                                            ttCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg                                                                                     <  1:2606604/150‑1 (MQ=255)
                                                              cGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTg                                                                                   >  2:1394831/1‑150 (MQ=255)
                                                                aTAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCg                                                                                 >  1:4724277/1‑150 (MQ=255)
                                                                 tAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGa                                                                                >  2:3070972/1‑150 (MQ=255)
                                                                   aaTGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGt                                                                              <  2:1102892/150‑1 (MQ=255)
                                                                     tGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGa                                                                            >  1:3564271/1‑150 (MQ=255)
                                                                     tGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGa                                                                            <  1:270688/150‑1 (MQ=255)
                                                                     tGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGa                                                                            <  1:1304264/150‑1 (MQ=255)
                                                                      gCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGAc                                                                           >  1:1373814/1‑150 (MQ=255)
                                                                       cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACa                                                                          >  2:2648633/1‑150 (MQ=255)
                                                                       cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACa                                                                          <  2:739355/150‑1 (MQ=255)
                                                                        aaGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACaa                                                                         >  2:4005150/1‑150 (MQ=255)
                                                                        aaGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACaa                                                                         >  2:1426811/1‑150 (MQ=255)
                                                                         aGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACaaa                                                                        >  1:3036693/1‑150 (MQ=255)
                                                                          gTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAAc                                                                       <  2:2096337/150‑1 (MQ=255)
                                                                          gTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAAc                                                                       >  1:3777054/1‑150 (MQ=255)
                                                                          gTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAAc                                                                       >  2:3376235/1‑150 (MQ=255)
                                                                            ggACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACAt                                                                     <  1:1675382/150‑1 (MQ=255)
                                                                             gACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATg                                                                    <  1:4422675/150‑1 (MQ=255)
                                                                             gACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATg                                                                    <  2:2022960/150‑1 (MQ=255)
                                                                               cTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGac                                                                  >  1:2500046/1‑150 (MQ=255)
                                                                                  gTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACt                                                               <  2:4502478/150‑1 (MQ=255)
                                                                                        aTCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAAt                                                         >  1:40290/1‑150 (MQ=255)
                                                                                          cGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGt                                                       <  2:1645828/150‑1 (MQ=255)
                                                                                           gACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTc                                                      >  2:889160/1‑150 (MQ=255)
                                                                                            aCTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCa                                                     <  1:1222594/150‑1 (MQ=255)
                                                                                            aCTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCa                                                     >  2:31376/1‑150 (MQ=255)
                                                                                             cTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCaa                                                    >  1:4477715/1‑150 (MQ=255)
                                                                                             cTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCaa                                                    >  2:4739308/1‑150 (MQ=255)
                                                                                               cATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCaaaa                                                  >  1:1794070/1‑150 (MQ=255)
                                                                                                  atTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAg                                               >  2:4208626/1‑150 (MQ=255)
                                                                                                    tACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAgcg                                             <  1:1569015/150‑1 (MQ=255)
                                                                                                    tACGATAATCCGTATACGATTAAGGGGATTATTAATGCTCGTGGGATTTTTATCAATTACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAtcg                                             >  2:1292085/1‑150 (MQ=255)
                                                                                                     aCGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAgcgc                                            >  2:3203870/1‑150 (MQ=255)
                                                                                                         tAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCtgat                                        <  2:2692017/150‑1 (MQ=255)
                                                                                                         tAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCtgat                                        >  1:1769238/1‑150 (MQ=255)
                                                                                                          aaTCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCtgatg                                       <  2:376835/150‑1 (MQ=255)
                                                                                                           aTCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCtgatga                                      <  2:2346693/150‑1 (MQ=255)
                                                                                                            tCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATATATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGAGACAAACATGACACTGAAAATGTCAAAAAAGCGCTgatgat                                     >  1:1156887/1‑150 (MQ=255)
                                                                                                              cGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGataa                                   <  2:1970944/150‑1 (MQ=255)
                                                                                                               gTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGataat                                  >  2:1600556/1‑150 (MQ=255)
                                                                                                                 cTACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGAtaataa                                >  1:2182692/1‑150 (MQ=255)
                                                                                                                  tACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATaaa                               >  1:4916028/1‑150 (MQ=255)
                                                                                                                  tACGATTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATaaa                               >  1:2998701/1‑150 (MQ=255)
                                                                                                                      aTTAAGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCt                           >  2:1865537/1‑150 (MQ=255)
                                                                                                                       ttAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTg                          <  2:815521/150‑1 (MQ=255)
                                                                                                                        tAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGa                         >  1:1537601/1‑150 (MQ=255)
                                                                                                                         aaGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGAt                        >  2:1774758/1‑150 (MQ=255)
                                                                                                                          aGGGGAGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATg                       <  1:10720/150‑1 (MQ=255)
                                                                                                                             ggAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCat                    >  2:933185/1‑150 (MQ=255)
                                                                                                                               aGTATTAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCatat                  >  2:5047877/1‑150 (MQ=255)
                                                                                                                                   ttAATGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATattatt              >  1:4936435/1‑150 (MQ=255)
                                                                                                                                     aaTGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTgt            >  2:2944884/1‑149 (MQ=255)
                                                                                                                                      aTGCGCGTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGAGCTGATGATAATAAATCATGATGCATATTATTggg           >  1:3130689/1‑150 (MQ=255)
                                                                                                                                         cgcgTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGc        <  1:1259031/150‑1 (MQ=255)
                                                                                                                                          gcgTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACACATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCt       <  1:2701801/150‑1 (MQ=255)
                                                                                                                                           cgTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCta      <  1:1999145/150‑1 (MQ=255)
                                                                                                                                            gTGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaa     >  1:3095091/1‑150 (MQ=255)
                                                                                                                                             tGGGATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaat    >  1:4183547/1‑150 (MQ=255)
                                                                                                                                              gggATTTTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCtaatt   <  1:701408/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATGACACTGAAAATGTCAAAAAAGCGCTGATGATAATAAATCCTGATGCATATTATTGGGGGCTAATTA  >  CP009273/1469733‑1470025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑