BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,512,088	G→T	6.2%	D62Y (GAT→TAT)	ydcY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,512,088	0	G	T	6.2%	1040.3 / 27.1	290	D62Y (GAT→TAT)	ydcY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (164/108); new base T (8/10); total (172/118)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 303 total aligned reads displayed.

TGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCG  >  CP009273/1511940‑1512237

tGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAagaaga                                                                                                                                                      >  2:3203873/1‑150 (MQ=255)

   cgcgATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGatat                                                                                                                                                   >  1:4848607/1‑150 (MQ=255)

   cgcgATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGatat                                                                                                                                                   >  2:485291/1‑150 (MQ=255)

         tGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgc                                                                                                                                             >  1:739513/1‑150 (MQ=255)

         tGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgc                                                                                                                                             >  2:559894/1‑150 (MQ=255)

         tGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgc                                                                                                                                             <  2:3912333/150‑1 (MQ=255)

          gAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcg                                                                                                                                            <  1:617440/150‑1 (MQ=255)

          gAAGAGAGCGTCATTGCCCATATGAACGAATAATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcg                                                                                                                                            <  2:1048655/150‑1 (MQ=255)

           tagagagCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcgc                                                                                                                                           <  2:4772831/149‑1 (MQ=255)

           aaGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcgc                                                                                                                                           >  2:1623633/1‑150 (MQ=255)

            agagagCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcgcg                                                                                                                                          >  2:2018346/1‑150 (MQ=255)

            agagagCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAgcgcg                                                                                                                                          <  2:4057911/150‑1 (MQ=255)

                agCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCAc                                                                                                                                      >  2:1527989/1‑150 (MQ=255)

                  cGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGa                                                                                                                                    >  2:4877507/1‑150 (MQ=255)

                   gTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGaa                                                                                                                                   >  1:22493/1‑150 (MQ=255)

                    tCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAAc                                                                                                                                  >  2:3778759/1‑150 (MQ=255)

                      aTTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAg                                                                                                                                >  1:3697118/1‑150 (MQ=255)

                       ttGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGt                                                                                                                               <  2:2403577/150‑1 (MQ=255)

                         gCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTa                                                                                                                             >  1:1178488/1‑150 (MQ=255)

                         gCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTa                                                                                                                             >  2:2754709/1‑150 (MQ=255)

                            cATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAAcc                                                                                                                          >  1:4899407/1‑150 (MQ=255)

                               atGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCaaa                                                                                                                       >  1:4281226/1‑150 (MQ=255)

                                 gAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAgg                                                                                                                     <  2:29642/150‑1 (MQ=255)

                                  aaCGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAggc                                                                                                                    <  2:2141377/150‑1 (MQ=255)

                                         tattaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCa                                                                                                             <  1:1035399/150‑1 (MQ=255)

                                         tattaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCa                                                                                                             <  1:3999329/150‑1 (MQ=255)

                                             aaTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCct                                                                                                         <  2:735843/150‑1 (MQ=255)

                                              aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCCCTAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                        >  2:2777712/1‑150 (MQ=255)

                                              aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                        >  2:182536/1‑150 (MQ=255)

                                              aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                        >  2:1377449/1‑150 (MQ=255)

                                               tCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctct                                                                                                       >  1:3644468/1‑150 (MQ=255)

                                                cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                      <  1:838804/150‑1 (MQ=255)

                                                cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                      >  2:4956920/1‑150 (MQ=255)

                                                cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                      >  2:1737166/1‑150 (MQ=255)

                                                   ccTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCACGCATAAAGAAGATATGGACGCGCGCCACGAACAGTTCACCAAAGGCGGCACCATCCTCTGAtt                                                                                                   >  2:794208/1‑150 (MQ=255)

                                                     tGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaa                                                                                                 >  2:2507523/1‑150 (MQ=255)

                                                     tGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaa                                                                                                 >  1:3281067/1‑150 (MQ=255)

                                                            tGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGc                                                                                          <  2:3275180/150‑1 (MQ=255)

                                                            tGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGc                                                                                          <  1:4959585/150‑1 (MQ=255)

                                                                      ttAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTac                                                                                >  2:385514/1‑150 (MQ=255)

                                                                       tAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTacc                                                                               <  1:1455932/150‑1 (MQ=255)

                                                                        aaGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTacca                                                                              >  1:468125/1‑150 (MQ=255)

                                                                         aGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaa                                                                             >  1:2885214/1‑150 (MQ=255)

                                                                            cGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacca                                                                          <  2:4751750/150‑1 (MQ=255)

                                                                             gggAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAg                                                                         <  1:3789200/150‑1 (MQ=255)

                                                                              ggAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAgg                                                                        <  1:265583/150‑1 (MQ=255)

                                                                                aaGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcg                                                                      >  1:5030649/1‑150 (MQ=255)

                                                                                 aGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcgc                                                                     >  1:1715061/1‑150 (MQ=255)

                                                                                 aGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcgc                                                                     <  2:3098171/150‑1 (MQ=255)

                                                                                 aGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcgc                                                                     <  2:3349198/150‑1 (MQ=255)

                                                                                   aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                   <  1:3415153/150‑1 (MQ=255)

                                                                                   aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                   >  2:3419795/1‑150 (MQ=255)

                                                                                   aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                   >  1:5159123/1‑150 (MQ=255)

                                                                                   aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                   <  2:437252/150‑1 (MQ=255)

                                                                                    tCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCaa                                                                  <  1:762826/150‑1 (MQ=255)

                                                                                       cTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACca                                                               <  1:3899174/150‑1 (MQ=255)

                                                                                       cTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACca                                                               >  1:2185003/1‑150 (MQ=255)

                                                                                        tACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACcac                                                              >  1:1614481/1‑150 (MQ=255)

                                                                                         acacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACcaca                                                             >  1:3126335/1‑150 (MQ=255)

                                                                                          cacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACaa                                                            >  2:3765654/1‑150 (MQ=255)

                                                                                          cacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACaa                                                            >  1:1664705/1‑150 (MQ=255)

                                                                                           acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                           <  1:939168/150‑1 (MQ=255)

                                                                                           acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                           >  2:3174415/1‑150 (MQ=255)

                                                                                           acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                           <  1:4145587/150‑1 (MQ=255)

                                                                                            cccAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGt                                                          >  2:363014/1‑150 (MQ=255)

                                                                                            cccAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGt                                                          >  2:3015968/1‑150 (MQ=255)

                                                                                                     aaCGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTgcg                                                 <  1:4416036/150‑1 (MQ=255)

                                                                                                       cGTCTGCGCACAGCGATCGCCCATCACGGTAGCAAGCATAAAGAATATATGGAAGCGATCCACTTACTGTTATCCAAAGGCGGCACCATCCTATGATTAAAATGAGCGTCTTGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGcc                                               <  2:2259880/150‑1 (MQ=255)

                                                                                                         tNTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCcac                                             >  1:96597/1‑150 (MQ=255)

                                                                                                         tCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCcac                                             >  2:51750/1‑150 (MQ=255)

                                                                                                           tGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaa                                           <  1:2961098/150‑1 (MQ=255)

                                                                                                            gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                          <  1:4471385/150‑1 (MQ=255)

                                                                                                            gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                          >  2:224284/1‑150 (MQ=255)

                                                                                                            gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                          <  1:5149180/150‑1 (MQ=255)

                                                                                                              gcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGTCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAAtt                                        <  2:562157/150‑1 (MQ=255)

                                                                                                                acaGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTgg                                      <  1:3384726/150‑1 (MQ=255)

                                                                                                                  aGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGc                                    >  2:133201/1‑150 (MQ=255)

                                                                                                                     gATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAAGTGGGCCGa                                 >  1:3089856/1‑150 (MQ=255)

                                                                                                                      aTCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaa                                <  2:3466115/150‑1 (MQ=255)

                                                                                                                      aTCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaa                                >  2:4462626/1‑150 (MQ=255)

                                                                                                                         gCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTATCCATAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaacaa                             <  1:48110/150‑1 (MQ=255)

                                                                                                                           ccATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACg                           >  2:706750/1‑150 (MQ=255)

                                                                                                                           ccATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACg                           <  2:3159254/150‑1 (MQ=255)

                                                                                                                            cATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGc                          >  1:4377617/1‑150 (MQ=255)

                                                                                                                             aTCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGcc                         <  1:4048961/150‑1 (MQ=255)

                                                                                                                               cACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCg                       <  1:2039590/150‑1 (MQ=255)

                                                                                                                               cACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCg                       <  1:893019/150‑1 (MQ=255)

                                                                                                                                  ggTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAg                    >  2:1202493/1‑150 (MQ=255)

                                                                                                                                  ggTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAg                    <  1:1794528/150‑1 (MQ=255)

                                                                                                                                   gTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGt                   <  1:3238458/150‑1 (MQ=255)

                                                                                                                                    tCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTg                  >  1:1939543/1‑150 (MQ=255)

                                                                                                                                     cGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTgg                 >  2:2013760/1‑150 (MQ=255)

                                                                                                                                         aGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACg             >  1:4781435/1‑150 (MQ=255)

                                                                                                                                          gCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGc            >  1:142500/1‑150 (MQ=255)

                                                                                                                                           cATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGcc           <  1:1524438/150‑1 (MQ=255)

                                                                                                                                            aTAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATGGGGCCGAACAACGCCCGTAGTGGTACGCCa          <  2:4194952/150‑1 (MQ=255)

                                                                                                                                                 gtagaTATGGAAGCGCGACACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACt     <  1:1241048/148‑1 (MQ=255)

                                                                                                                                                  aagaTATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTg    <  2:4905216/150‑1 (MQ=255)

                                                                                                                                                   agaTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGc   >  2:191275/1‑150 (MQ=255)

                                                                                                                                                    gaTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCg  >  1:2505327/1‑150 (MQ=255)

TGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCG  >  CP009273/1511940‑1512237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`