Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,512,106 G→T 79.6% E68* (GAA→TAA)  ydcY → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,512,1060GT79.6% 538.0 / 175.2 295E68* (GAA→TAA) ydcYuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base T (134/101);  total (164/131)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 295 total aligned reads displayed.

CGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAT  >  CP009273/1511958‑1512252
                                                                                                                                                    |                                                                                                                                                  
cGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGa                                                                                                                                                   >  2:4877507/1‑150 (MQ=255)
 gTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTaa                                                                                                                                                  <  2:3871585/150‑1 (MQ=255)
  tCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAAc                                                                                                                                                 >  2:3778752/1‑150 (MQ=255)
    aTTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAg                                                                                                                                               >  1:3697118/1‑150 (MQ=255)
        cccATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTaa                                                                                                                                           >  1:715026/1‑150 (MQ=255)
         ccATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAAc                                                                                                                                          <  1:1070582/150‑1 (MQ=255)
          cATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAAcc                                                                                                                                         <  2:686623/150‑1 (MQ=255)
           atatGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCa                                                                                                                                        <  1:3180722/150‑1 (MQ=255)
                aaCGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAggc                                                                                                                                   >  2:2727657/1‑150 (MQ=255)
                 aCGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAggcg                                                                                                                                  <  1:1542959/150‑1 (MQ=255)
                   gAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTTCACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAggcggc                                                                                                                                <  1:4880759/150‑1 (MQ=255)
                       tattaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCa                                                                                                                            <  1:3999329/150‑1 (MQ=255)
                       tattaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCa                                                                                                                            >  1:1750617/1‑150 (MQ=255)
                          taATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATcc                                                                                                                         >  1:430796/1‑150 (MQ=255)
                           aaTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCCAAGGCGGCACCATCct                                                                                                                        >  2:132478/1‑150 (MQ=255)
                            aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                                       >  2:1377438/1‑150 (MQ=255)
                             tCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTCACAGTTAACCAAAGGCGGCACCATCctct                                                                                                                      >  2:1787727/1‑150 (MQ=255)
                             tCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctct                                                                                                                      >  1:3644468/1‑150 (MQ=255)
                              cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                                     >  2:4956920/1‑150 (MQ=255)
                              cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                                     <  1:838804/150‑1 (MQ=255)
                                   tGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaa                                                                                                                >  2:3000175/1‑150 (MQ=255)
                                   tGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGAGATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaa                                                                                                                >  2:3449891/1‑150 (MQ=255)
                                        gATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGa                                                                                                           >  2:2767164/1‑150 (MQ=255)
                                         aTGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAg                                                                                                          >  2:2450996/1‑150 (MQ=255)
                                          tGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGc                                                                                                         <  2:3144941/150‑1 (MQ=255)
                                          tGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGc                                                                                                         >  2:3366337/1‑150 (MQ=255)
                                          tGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGc                                                                                                         <  1:4959564/150‑1 (MQ=255)
                                             cgcgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTACCCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTc                                                                                                      <  1:4383428/150‑1 (MQ=255)
                                              gcgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCt                                                                                                     <  2:1873388/150‑1 (MQ=255)
                                               cgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTg                                                                                                    >  1:1312967/1‑150 (MQ=255)
                                                ggAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTgg                                                                                                   >  1:202217/1‑150 (MQ=255)
                                                ggAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTgg                                                                                                   >  1:2213985/1‑150 (MQ=255)
                                                     tAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTacc                                                                                              <  1:1455932/150‑1 (MQ=255)
                                                      aaGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTacca                                                                                             >  1:468125/1‑150 (MQ=255)
                                                          cGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacca                                                                                         <  1:1648052/150‑1 (MQ=255)
                                                          cGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacca                                                                                         <  2:4751750/150‑1 (MQ=255)
                                                           gggAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAg                                                                                        <  1:3789200/150‑1 (MQ=255)
                                                             gAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgc                                                                                      <  2:548251/150‑1 (MQ=255)
                                                              aaGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCCCCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcg                                                                                     >  2:846174/1‑150 (MQ=255)
                                                               aGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGgcgc                                                                                    >  1:1715061/1‑150 (MQ=255)
                                                                 aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                                  >  2:3816588/1‑150 (MQ=255)
                                                                 aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                                  >  1:5159123/1‑150 (MQ=255)
                                                                 aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                                  <  1:3415153/150‑1 (MQ=255)
                                                                 aTCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCa                                                                                  <  2:437296/150‑1 (MQ=255)
                                                                  tCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCaa                                                                                 <  1:762826/150‑1 (MQ=255)
                                                                   cGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCTAc                                                                                <  1:4160329/150‑1 (MQ=255)
                                                                     cTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACca                                                                              <  1:3899174/150‑1 (MQ=255)
                                                                       acacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACcaca                                                                            >  1:3126335/1‑150 (MQ=255)
                                                                         acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                                          <  1:939168/150‑1 (MQ=255)
                                                                         acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                                          <  2:2886076/150‑1 (MQ=255)
                                                                         acCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAg                                                                          >  2:3174393/1‑150 (MQ=255)
                                                                          cccAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGt                                                                         >  2:363014/1‑150 (MQ=255)
                                                                            cagcagCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCAACCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTaa                                                                       >  2:727143/1‑150 (MQ=255)
                                                                             agcagcAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTaaa                                                                      <  2:3309972/150‑1 (MQ=255)
                                                                              gcagcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGACGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTaaaa                                                                     >  1:1183267/1‑150 (MQ=255)
                                                                               cagcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTACAAt                                                                    >  1:2064108/1‑150 (MQ=255)
                                                                                agcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAAtt                                                                   >  2:4277927/1‑150 (MQ=255)
                                                                                   aaCGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCGTCGGATTAACATGTGCGTCTGGCTAGCAACCAGGCGCCAACCTCAAGTAAATGTgcg                                                                >  2:4821118/1‑150 (MQ=255)
                                                                                   aaCGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTgcg                                                                >  2:1713180/1‑150 (MQ=255)
                                                                                      gTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGAAAAATTGCGCca                                                             >  1:3042670/1‑150 (MQ=255)
                                                                                       tNTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCcac                                                            >  1:96597/1‑150 (MQ=255)
                                                                                         tGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaa                                                          >  2:2284998/1‑150 (MQ=255)
                                                                                          gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                                         <  1:4471385/150‑1 (MQ=255)
                                                                                          gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                                         >  2:224326/1‑150 (MQ=255)
                                                                                            gcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAAtt                                                       <  2:1992337/150‑1 (MQ=255)
                                                                                             cacaGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTg                                                      >  2:2351213/1‑150 (MQ=255)
                                                                                              acaGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTgg                                                     <  1:3384726/150‑1 (MQ=255)
                                                                                                 gCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGcc                                                  >  1:3273434/1‑150 (MQ=255)
                                                                                                  cGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCg                                                 >  1:187114/1‑150 (MQ=255)
                                                                                                   gATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGa                                                >  1:2929444/1‑150 (MQ=255)
                                                                                                     tCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCAGAAGTAAAATTGCGCCACAAATTGGGCCGaac                                              <  2:3710128/150‑1 (MQ=255)
                                                                                                       gCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTATCCATAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaacaa                                            <  1:48110/150‑1 (MQ=255)
                                                                                                           aTCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGcc                                        <  1:4048961/150‑1 (MQ=255)
                                                                                                           aTCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGcc                                        >  2:3491561/1‑150 (MQ=255)
                                                                                                             cACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCg                                      <  1:893019/150‑1 (MQ=255)
                                                                                                                ggTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAg                                   >  2:3195317/1‑150 (MQ=255)
                                                                                                                 gTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGt                                  >  1:249892/1‑150 (MQ=255)
                                                                                                                  tCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTg                                 >  2:1564604/1‑150 (MQ=255)
                                                                                                                   cGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTgg                                >  2:2013760/1‑150 (MQ=255)
                                                                                                                       aGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACg                            >  1:4781435/1‑150 (MQ=255)
                                                                                                                        gCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGc                           >  1:142500/1‑150 (MQ=255)
                                                                                                                           tAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCAt                        >  1:5021625/1‑150 (MQ=255)
                                                                                                                            aaaGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATg                       <  2:753425/150‑1 (MQ=255)
                                                                                                                             aagaagATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGa                      <  2:4479207/150‑1 (MQ=255)
                                                                                                                                aagaTATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTg                   <  2:4905216/150‑1 (MQ=255)
                                                                                                                                  gaTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCg                 <  2:2213985/150‑1 (MQ=255)
                                                                                                                                    tatGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGa               <  1:2754709/150‑1 (MQ=255)
                                                                                                                                    tatGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGa               <  2:25172/150‑1 (MQ=255)
                                                                                                                                    tatGGAAGCGCGCCACTAACAGTTAACCAAAGGCCGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGa               >  1:2608609/1‑150 (MQ=255)
                                                                                                                                      tGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAg             <  2:1178488/150‑1 (MQ=255)
                                                                                                                                        gAAGCGCGCCACTAACAGTTAACCACAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGat           <  2:4848607/150‑1 (MQ=255)
                                                                                                                                         aaGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGata          >  2:4156865/1‑150 (MQ=255)
                                                                                                                                            cgcgcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACACGTAACATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATcc       <  1:5136087/150‑1 (MQ=255)
                                                                                                                                            cgcgcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATcc       >  2:1039537/1‑150 (MQ=255)
                                                                                                                                             gcgcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCa      <  2:3096358/150‑1 (MQ=255)
                                                                                                                                             gcgcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCa      >  2:5054720/1‑150 (MQ=255)
                                                                                                                                               gcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGc    >  2:2141114/1‑150 (MQ=255)
                                                                                                                                                ccACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCa   <  1:1000312/150‑1 (MQ=255)
                                                                                                                                                 cACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAt  >  2:4182773/1‑150 (MQ=255)
                                                                                                                                                 cACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAt  >  1:2833837/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAT  >  CP009273/1511958‑1512252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑