Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,531,029 G→T 100% R180S (CGC→AGC)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,531,0290GT97.1% 921.5 / 12.9 308R180S (CGC→AGC) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base T (160/139);  total (164/144)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 313 total aligned reads displayed.

CTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCG  >  CP009273/1530882‑1531175
                                                                                                                                                   |                                                                                                                                                  
  cccGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGgtg                                                                                                                                                <  2:268586/150‑1 (MQ=255)
   tcGCACTGATGTCGAAATAATGCTGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGCTGACGCGGCGAGCTGTCACACGCCGTGGCGTTAGAGGCAAGACACGGCCCCTGTTCCTCTGCGCACACTGCATCAAGCGCCGGGCTGgggt                                                                                                                                               >  2:5103845/2‑150 (MQ=255)
    cGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTc                                                                                                                                              >  1:4703141/1‑150 (MQ=255)
       aCTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATc                                                                                                                                           <  1:3430106/150‑1 (MQ=255)
        cTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcg                                                                                                                                          <  2:3151917/150‑1 (MQ=255)
        cTGATGTCGACATAGTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcg                                                                                                                                          <  2:3993912/150‑1 (MQ=255)
           aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCgcgc                                                                                                                                       >  2:3385909/1‑150 (MQ=255)
            tGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGct                                                                                                                                      >  2:4918698/1‑150 (MQ=255)
             gTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctc                                                                                                                                     >  1:3262765/1‑150 (MQ=255)
                 aCATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCg                                                                                                                                 >  1:4975002/1‑150 (MQ=255)
                  cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACGTCACCTGATCCTCTTCCCCCACTGCATCACGCGCCTGCCTCGTGTCATCGCGCTCTTCGc                                                                                                                                >  2:4406085/1‑150 (MQ=255)
                   atatTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCt                                                                                                                               <  1:1280762/150‑1 (MQ=255)
                   atatTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAAGAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCt                                                                                                                               <  2:2464082/150‑1 (MQ=255)
                     atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGt                                                                                                                             >  2:1474717/1‑150 (MQ=255)
                       tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGttt                                                                                                                           >  2:3565306/1‑150 (MQ=255)
                          ggcgCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCTCACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACt                                                                                                                        <  2:1497913/150‑1 (MQ=255)
                          ggcgCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACt                                                                                                                        <  2:3284828/150‑1 (MQ=255)
                              cgACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                    >  2:616131/1‑150 (MQ=255)
                               gACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGttt                                                                                                                   <  1:3689440/150‑1 (MQ=255)
                                aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtttt                                                                                                                  <  2:3247442/150‑1 (MQ=255)
                                aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtttt                                                                                                                  <  2:1862319/150‑1 (MQ=255)
                                aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtttt                                                                                                                  <  2:532369/150‑1 (MQ=255)
                                 cATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtg                                                                                                                 >  2:3670515/1‑150 (MQ=255)
                                   tCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgtg                                                                                                               >  1:546111/1‑150 (MQ=255)
                                     cTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGac                                                                                                             <  1:3224370/150‑1 (MQ=255)
                                     cTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGac                                                                                                             <  1:4736646/150‑1 (MQ=255)
                                      tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGaca                                                                                                            <  2:827298/150‑1 (MQ=255)
                                       ggCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                           >  2:3414983/1‑150 (MQ=255)
                                         cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                         >  2:1029421/1‑150 (MQ=255)
                                         cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                         >  2:4200639/1‑150 (MQ=255)
                                          tAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGt                                                                                                        >  2:4582991/1‑150 (MQ=255)
                                          tAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGt                                                                                                        <  1:4724230/150‑1 (MQ=255)
                                              gCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt                                                                                                    <  1:2277684/150‑1 (MQ=255)
                                                gTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTg                                                                                                  <  2:4624437/150‑1 (MQ=255)
                                                gTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTg                                                                                                  >  2:2997413/1‑150 (MQ=255)
                                                 tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGa                                                                                                 <  1:2361581/150‑1 (MQ=255)
                                                 tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGa                                                                                                 >  2:642412/1‑150 (MQ=255)
                                                  cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaa                                                                                                >  1:155829/1‑150 (MQ=255)
                                                     tGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAgg                                                                                             <  2:3319034/150‑1 (MQ=255)
                                                       atatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                           >  2:2199231/1‑150 (MQ=255)
                                                       atatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGGTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                           >  2:2315199/1‑150 (MQ=255)
                                                          ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCt                                                                                        <  2:410066/150‑1 (MQ=255)
                                                               tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCg                                                                                   <  2:4855812/150‑1 (MQ=255)
                                                               tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCg                                                                                   >  2:2729696/1‑150 (MQ=255)
                                                               tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCg                                                                                   >  2:4484214/1‑150 (MQ=255)
                                                                aaGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGc                                                                                  >  1:5075739/1‑150 (MQ=255)
                                                                   cggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAg                                                                               >  2:4713555/1‑150 (MQ=255)
                                                                    ggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTGTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGt                                                                              >  2:1906709/1‑150 (MQ=255)
                                                                           tGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                       >  2:2801160/1‑150 (MQ=255)
                                                                           tGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                       >  2:867822/1‑150 (MQ=255)
                                                                             tCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCaga                                                                     >  2:2756597/1‑150 (MQ=255)
                                                                             tCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCaga                                                                     >  1:3793194/1‑150 (MQ=255)
                                                                             tCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAGCGCGCGCTTCGCTGTTTACTGGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCaga                                                                     >  2:2130675/1‑150 (MQ=255)
                                                                              cacaCGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCagag                                                                    >  1:2068327/1‑150 (MQ=255)
                                                                                 acGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAt                                                                 <  2:4176144/150‑1 (MQ=255)
                                                                                   gCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCa                                                               <  2:4763867/150‑1 (MQ=255)
                                                                                    ccGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCaa                                                              >  1:2139884/1‑150 (MQ=255)
                                                                                     cGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCaaa                                                             >  1:29074/1‑150 (MQ=255)
                                                                                      gTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaac                                                            >  2:1291393/1‑150 (MQ=255)
                                                                                       tGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaaca                                                           <  1:251460/150‑1 (MQ=255)
                                                                                         gCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaacaac                                                         <  1:414365/150‑1 (MQ=255)
                                                                                          cGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcg                                                        >  1:1216928/1‑150 (MQ=255)
                                                                                          cGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcg                                                        >  1:71189/1‑150 (MQ=255)
                                                                                             tATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGt                                                     <  1:616131/150‑1 (MQ=255)
                                                                                              aTCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                    <  1:3128124/150‑1 (MQ=255)
                                                                                              aTCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                    <  1:1593384/150‑1 (MQ=255)
                                                                                                cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTAcc                                                  <  1:30280/150‑1 (MQ=255)
                                                                                                  tCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCAc                                                >  1:3001970/1‑150 (MQ=255)
                                                                                                   cAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACg                                               >  2:693213/1‑150 (MQ=255)
                                                                                                    aaTAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACgg                                              >  2:1606956/1‑150 (MQ=255)
                                                                                                        aaCTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGcc                                          >  1:5060896/1‑150 (MQ=255)
                                                                                                           ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                       <  1:1029421/150‑1 (MQ=255)
                                                                                                             cACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAACAAACAACGCGAACCACGGTGCCTcgcg                                     >  1:3204669/1‑150 (MQ=255)
                                                                                                               ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                   <  1:3069655/150‑1 (MQ=255)
                                                                                                               ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                   >  1:2844766/1‑150 (MQ=255)
                                                                                                                cTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttg                                  >  1:4386759/1‑150 (MQ=255)
                                                                                                                  ggtCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                                <  1:2796312/148‑1 (MQ=255)
                                                                                                                  gTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                                >  2:1314432/1‑150 (MQ=255)
                                                                                                                       tctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAg                           <  1:1942865/150‑1 (MQ=255)
                                                                                                                        ctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTACCTCGCGTTGTTTTAAGc                          >  1:3927375/1‑150 (MQ=255)
                                                                                                                          taccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGATTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGACAGTTGCAGCAGAGCATCAAACAACGAGAACCACGGTGCCTCGCGTTGTTTTAAGCGa                        >  1:2655851/3‑150 (MQ=255)
                                                                                                                          tCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                        >  1:2946882/1‑150 (MQ=255)
                                                                                                                          tCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                        <  1:842328/150‑1 (MQ=255)
                                                                                                                           cccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAc                       >  1:756804/1‑150 (MQ=255)
                                                                                                                            ccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                      <  1:1100583/150‑1 (MQ=255)
                                                                                                                                acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCa                  <  2:2872583/150‑1 (MQ=255)
                                                                                                                                acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCa                  >  1:4485091/1‑150 (MQ=255)
                                                                                                                                 cgtcATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTGACGTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCaa                 <  2:2029982/147‑1 (MQ=255)
                                                                                                                                  tGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg                >  2:4315176/1‑150 (MQ=255)
                                                                                                                                   gCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc               >  1:5139732/1‑150 (MQ=255)
                                                                                                                                   gCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc               >  1:107675/1‑150 (MQ=255)
                                                                                                                                    cATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCGGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCa              >  2:2076929/1‑150 (MQ=255)
                                                                                                                                     aTCACGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg             <  2:2487523/150‑1 (MQ=255)
                                                                                                                                       cAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGATTAATTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg           >  2:1779003/1‑150 (MQ=255)
                                                                                                                                        aaGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGc          >  2:345877/1‑150 (MQ=255)
                                                                                                                                         aGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg         <  1:1672437/150‑1 (MQ=255)
                                                                                                                                          gcgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGa        <  2:4996520/150‑1 (MQ=255)
                                                                                                                                          gcgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGa        >  1:1429123/1‑150 (MQ=255)
                                                                                                                                            gcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg      >  1:3565690/1‑150 (MQ=255)
                                                                                                                                               tGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATc   <  2:1151614/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCG  >  CP009273/1530882‑1531175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑