Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,570,428 G→T 100% I483I (ATC→ATA)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,570,4280GT99.6% 729.9 / ‑4.3 230I483I (ATC→ATA) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (123/106);  total (124/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

ATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGT  >  CP009273/1570281‑1570573
                                                                                                                                                   |                                                                                                                                                 
    ccTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtg                                                                                                                                             <  1:3607893/150‑1 (MQ=255)
       gATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTgg                                                                                                                                          <  1:4382576/150‑1 (MQ=255)
        aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTACCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATCTTGTGGt                                                                                                                                         >  2:4334552/1‑150 (MQ=255)
           ccGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTg                                                                                                                                      >  1:1077330/1‑150 (MQ=255)
             gATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGAc                                                                                                                                    <  2:1250420/150‑1 (MQ=255)
              aTTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACa                                                                                                                                   <  1:1485681/150‑1 (MQ=255)
              aTTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACa                                                                                                                                   >  2:3919298/1‑150 (MQ=255)
                   gTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAg                                                                                                                              <  2:3593400/150‑1 (MQ=255)
                       aGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCGGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgt                                                                                                                          <  2:617153/150‑1 (MQ=255)
                         cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtca                                                                                                                        <  1:1883739/150‑1 (MQ=255)
                         cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtca                                                                                                                        >  1:450038/1‑150 (MQ=255)
                          tATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcat                                                                                                                       >  1:3938996/1‑150 (MQ=255)
                          tATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcat                                                                                                                       <  1:1910539/150‑1 (MQ=255)
                            ttGCGTATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatag                                                                                                                     <  2:237046/150‑1 (MQ=255)
                            ttGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatag                                                                                                                     <  2:3557725/150‑1 (MQ=255)
                               cGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAACGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtca                                                                                                                  <  2:4294920/150‑1 (MQ=255)
                               cGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtca                                                                                                                  >  1:4198958/1‑150 (MQ=255)
                                ggATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtcat                                                                                                                 <  1:1398217/150‑1 (MQ=255)
                                 gATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtcata                                                                                                                <  2:3251827/150‑1 (MQ=255)
                                   tatCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATAcc                                                                                                              >  2:1407269/1‑150 (MQ=255)
                                      cACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCa                                                                                                           <  2:4993238/150‑1 (MQ=255)
                                        cGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                         <  2:2430083/150‑1 (MQ=255)
                                            aTCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCgg                                                                                                     >  1:3926389/1‑150 (MQ=255)
                                             tCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCggg                                                                                                    >  1:2938960/1‑150 (MQ=255)
                                                cATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAt                                                                                                 <  1:4660165/150‑1 (MQ=255)
                                                cATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAt                                                                                                 <  1:1892146/150‑1 (MQ=255)
                                                    tCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAAt                                                                                             <  1:284287/150‑1 (MQ=255)
                                                      aaGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGa                                                                                           <  2:3811300/150‑1 (MQ=255)
                                                       aGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                          >  1:2030960/1‑150 (MQ=255)
                                                         aaTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGacac                                                                                        >  2:373540/1‑150 (MQ=255)
                                                             ttCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTa                                                                                    <  2:38840/150‑1 (MQ=255)
                                                              tCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTAc                                                                                   <  2:4176114/150‑1 (MQ=255)
                                                                ccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGc                                                                                 >  2:1984904/1‑150 (MQ=255)
                                                                ccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGc                                                                                 >  2:1169868/1‑150 (MQ=255)
                                                                  gCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCa                                                                               <  2:3355849/150‑1 (MQ=255)
                                                                    cATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGc                                                                             <  2:1632738/150‑1 (MQ=255)
                                                                        gTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAAt                                                                         <  2:4552018/150‑1 (MQ=255)
                                                                        gTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAAt                                                                         >  2:3241882/1‑150 (MQ=255)
                                                                         tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGAGGGGATCTATTGTGGTTTGACCGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGAAACATTACGACAGCTAATg                                                                        >  2:3340257/1‑150 (MQ=255)
                                                                          aaCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGc                                                                       >  1:337547/1‑150 (MQ=255)
                                                                          aaCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGc                                                                       <  2:3037665/150‑1 (MQ=255)
                                                                            cGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGa                                                                     >  1:4165452/1‑150 (MQ=255)
                                                                             ggTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGAt                                                                    <  1:1588093/150‑1 (MQ=255)
                                                                              gTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATc                                                                   >  1:1708252/1‑150 (MQ=255)
                                                                              gTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATc                                                                   <  1:2776702/150‑1 (MQ=255)
                                                                                 ataACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                                >  2:1709041/1‑150 (MQ=255)
                                                                                    aCCTGCTGTAATCATTGAGGTTTGATTATCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGAAAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCat                                                             <  2:2946189/150‑1 (MQ=255)
                                                                                    aCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCat                                                             <  2:1261818/150‑1 (MQ=255)
                                                                                       tgctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCAtata                                                          <  1:2538464/150‑1 (MQ=255)
                                                                                         ctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACa                                                        >  1:3328511/1‑150 (MQ=255)
                                                                                          tgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAg                                                       >  2:1500440/1‑150 (MQ=255)
                                                                                             aaTCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgt                                                    >  1:4370696/1‑150 (MQ=255)
                                                                                             aaTCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgt                                                    <  2:2377846/150‑1 (MQ=255)
                                                                                               tCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtgt                                                  >  1:849889/1‑150 (MQ=255)
                                                                                               tCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtgt                                                  >  2:1676685/1‑150 (MQ=255)
                                                                                               tCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtgt                                                  >  2:3714767/1‑150 (MQ=255)
                                                                                                  ttGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGt                                               >  1:3840080/1‑150 (MQ=255)
                                                                                                   tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGtt                                              >  2:5124326/1‑150 (MQ=255)
                                                                                                   tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGtt                                              >  1:1733474/1‑150 (MQ=255)
                                                                                                        tttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCaa                                         <  2:1878247/150‑1 (MQ=255)
                                                                                                          tGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAtt                                       >  1:4045605/1‑150 (MQ=255)
                                                                                                            aTTAGCAAAAATATCCCATTCCGTCATACAGCGCGGGGATCTATTGTTGTGTGACAGATCGTTGTCCTTGTCATACAGCCCGCCGGGCCTTAATGACTCATTACGCCAGCTCGTGCGCTCCGCCCTCTCCGGCGGGTAGTTGTCAATTca                                     >  2:4657387/1‑149 (MQ=255)
                                                                                                            aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                     <  1:4739330/150‑1 (MQ=255)
                                                                                                             ttAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTccc                                    <  1:3114539/150‑1 (MQ=255)
                                                                                                              tAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCa                                   <  2:4790664/150‑1 (MQ=255)
                                                                                                                gCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                                 <  2:2002439/150‑1 (MQ=255)
                                                                                                                gCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                                 >  2:2787184/1‑150 (MQ=255)
                                                                                                                 cAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggc                                <  2:5058452/150‑1 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                           >  1:3500452/1‑150 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                           <  2:2179861/150‑1 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                           <  2:1051178/150‑1 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                           <  1:68896/150‑1 (MQ=255)
                                                                                                                       tatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                          >  1:57273/1‑150 (MQ=255)
                                                                                                                        atCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGccttt                         <  1:3471334/150‑1 (MQ=255)
                                                                                                                          cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttac                       >  1:496966/1‑150 (MQ=255)
                                                                                                                          cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttac                       >  2:3318258/1‑150 (MQ=255)
                                                                                                                            cATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                     <  1:3736243/150‑1 (MQ=255)
                                                                                                                             aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                    <  2:4223654/150‑1 (MQ=255)
                                                                                                                             aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                    <  2:2042651/150‑1 (MQ=255)
                                                                                                                             aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                    <  1:5081778/150‑1 (MQ=255)
                                                                                                                              ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttt                   >  1:1955367/1‑150 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                  <  2:2523409/150‑1 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                  >  1:1915935/1‑150 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                  >  2:4067023/1‑150 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                  >  1:1058709/1‑150 (MQ=255)
                                                                                                                                ccGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTAt                 <  2:723425/150‑1 (MQ=255)
                                                                                                                                ccGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTAt                 <  2:22615/150‑1 (MQ=255)
                                                                                                                                ccGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTAt                 >  2:1244488/1‑150 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATg                >  1:2242046/1‑150 (MQ=255)
                                                                                                                                  gTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg               <  2:4370015/150‑1 (MQ=255)
                                                                                                                                  gTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg               <  1:2396361/150‑1 (MQ=255)
                                                                                                                                  gTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg               >  1:3930389/1‑150 (MQ=255)
                                                                                                                                       aaaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat          >  1:2920108/1‑150 (MQ=255)
                                                                                                                                       aaaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat          <  2:4029517/150‑1 (MQ=255)
                                                                                                                                         aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg        >  2:2668214/1‑150 (MQ=255)
                                                                                                                                         aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg        <  1:3819111/150‑1 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg   >  1:4951792/1‑150 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg   >  1:4612455/1‑150 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg   >  1:1158996/1‑150 (MQ=255)
                                                                                                                                               gggATAGATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCAGATACAGCGTGTCGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtctggt  >  2:1464576/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGT  >  CP009273/1570281‑1570573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑