Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,574,523 T→A 100% L175F (TTA→TTT)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,574,5230TA99.5% 633.6 / ‑4.5 187L175F (TTA→TTT) ydeMputative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (92/94);  total (93/94)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTA  >  CP009273/1574376‑1574669
                                                                                                                                                   |                                                                                                                                                  
ggTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTaaa                                                                                                                                                  <  1:4485747/150‑1 (MQ=255)
      cATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATg                                                                                                                                            >  1:955700/1‑150 (MQ=255)
       aTAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGa                                                                                                                                           >  2:227382/1‑150 (MQ=255)
       aTAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGa                                                                                                                                           >  2:1259910/1‑150 (MQ=255)
        tAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGAt                                                                                                                                          >  1:2776110/1‑150 (MQ=255)
          aaCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATaa                                                                                                                                        >  2:4885479/1‑150 (MQ=255)
             ttGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATCAAcc                                                                                                                                     >  2:1378635/1‑150 (MQ=255)
                ccATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCa                                                                                                                                  <  1:4635134/150‑1 (MQ=255)
                  aTAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAag                                                                                                                                >  1:2134720/1‑150 (MQ=255)
                  aTAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTAAag                                                                                                                                >  2:4397007/1‑150 (MQ=255)
                    aaGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAagag                                                                                                                              <  2:4770811/150‑1 (MQ=255)
                     aGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                             <  1:4206840/150‑1 (MQ=255)
                        cGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTa                                                                                                                          <  2:2307303/150‑1 (MQ=255)
                         gTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTaa                                                                                                                         <  1:5028427/150‑1 (MQ=255)
                         gTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTaa                                                                                                                         <  2:3386023/150‑1 (MQ=255)
                         gTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATAAGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTaa                                                                                                                         >  1:2478567/1‑150 (MQ=255)
                           gtgAGGCACAGATAAATCAATGATACTGAATGTTTTCTCACTATGACCACTGAAATCAATATTCGTCTTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAATAGGGTAATg                                                                                                                       <  2:3402549/148‑1 (MQ=255)
                           gggAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATg                                                                                                                       >  1:3480593/1‑150 (MQ=255)
                           gggAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATg                                                                                                                       <  2:2456960/150‑1 (MQ=255)
                           gggAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATg                                                                                                                       >  1:3780095/1‑150 (MQ=255)
                            ggAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATgg                                                                                                                      >  1:4694017/1‑150 (MQ=255)
                               ggCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACa                                                                                                                   <  2:597390/150‑1 (MQ=255)
                               ggCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACa                                                                                                                   >  1:4933045/1‑150 (MQ=255)
                                gCACAGAAAAATCAATGATCCGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACAt                                                                                                                  <  1:3344178/150‑1 (MQ=255)
                                 cacaGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACAtt                                                                                                                 >  1:3278335/1‑150 (MQ=255)
                                    aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAAc                                                                                                              >  1:1602330/1‑150 (MQ=255)
                                    aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAAc                                                                                                              <  2:4730966/150‑1 (MQ=255)
                                      aaaaaTCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGt                                                                                                            >  1:3291034/1‑150 (MQ=255)
                                          aTCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatt                                                                                                        <  2:4724717/150‑1 (MQ=255)
                                           tCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAAGTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatta                                                                                                       <  2:4557487/150‑1 (MQ=255)
                                             aaTGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAAt                                                                                                     >  1:4741526/1‑150 (MQ=255)
                                               tGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGa                                                                                                   <  2:2824826/150‑1 (MQ=255)
                                                 aTACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACg                                                                                                 >  1:263951/1‑150 (MQ=255)
                                                    cGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGtt                                                                                              <  2:827981/150‑1 (MQ=255)
                                                     ggAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTa                                                                                             <  2:1638845/150‑1 (MQ=255)
                                                           tgtTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTAt                                                                                       <  1:1991382/150‑1 (MQ=255)
                                                               ctcACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTaaa                                                                                   <  2:2927138/150‑1 (MQ=255)
                                                                tcACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAAc                                                                                  <  1:2083406/150‑1 (MQ=255)
                                                                  aCTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAActc                                                                                >  1:3870622/1‑150 (MQ=255)
                                                                    tATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTa                                                                              <  1:1161822/150‑1 (MQ=255)
                                                                    tATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTa                                                                              <  1:2364167/150‑1 (MQ=255)
                                                                     aTGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTAc                                                                             >  2:888410/1‑150 (MQ=255)
                                                                     aTGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTAc                                                                             >  2:5034030/1‑150 (MQ=255)
                                                                     aTGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTAc                                                                             >  2:2161059/1‑150 (MQ=255)
                                                                     aTGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAAAGATAAACCTCAAGAGGGTAATGGACATTAACTTTATTAATGACGGTTAACGTATTAAACTCTAc                                                                             >  1:1760655/1‑150 (MQ=255)
                                                                       gACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTANCGTTATTAATGACGGTTAACGTATTAAACTCTACtt                                                                           <  1:2678310/150‑1 (MQ=255)
                                                                         ccACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGa                                                                         >  1:3954983/1‑150 (MQ=255)
                                                                          cACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGat                                                                        >  1:4569652/1‑150 (MQ=255)
                                                                           aCTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATTTTTAATGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGata                                                                       <  2:900598/150‑1 (MQ=255)
                                                                               aaaTCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATAAGAt                                                                   >  1:442123/1‑150 (MQ=255)
                                                                                  tCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAGCGTATTAAACTCTACTTGATATGATTTc                                                                <  2:4892450/150‑1 (MQ=255)
                                                                                  tCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTc                                                                <  2:3056044/150‑1 (MQ=255)
                                                                                     atatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGa                                                             >  1:707700/1‑150 (MQ=255)
                                                                                     atatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGa                                                             >  2:2953907/1‑150 (MQ=255)
                                                                                       atTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACg                                                           >  2:4275415/1‑150 (MQ=255)
                                                                                           ggCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCg                                                       >  1:4885954/1‑150 (MQ=255)
                                                                                           ggCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATAAAACTCTACTTGATATGATTTCAGACGCTCg                                                       <  2:4599050/150‑1 (MQ=255)
                                                                                             cGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGAt                                                     <  2:3291034/150‑1 (MQ=255)
                                                                                              gTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATg                                                    <  2:1336720/150‑1 (MQ=255)
                                                                                              gTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATg                                                    >  1:2771157/1‑150 (MQ=255)
                                                                                               tCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATgg                                                   >  2:1765051/1‑150 (MQ=255)
                                                                                               tCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATgg                                                   <  1:1798401/150‑1 (MQ=255)
                                                                                                cccGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGgc                                                  >  2:2087206/1‑150 (MQ=255)
                                                                                                    gTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGgctgct                                              <  1:2602126/150‑1 (MQ=255)
                                                                                                        cTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATACACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCa                                          <  2:930059/150‑1 (MQ=255)
                                                                                                        cTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCa                                          >  2:2437616/1‑150 (MQ=255)
                                                                                                        cTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCa                                          >  2:1895778/1‑150 (MQ=255)
                                                                                                           gCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACtt                                       <  1:4490022/150‑1 (MQ=255)
                                                                                                             aaTTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACtttt                                     >  1:4042874/1‑150 (MQ=255)
                                                                                                             aaTTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACtttt                                     <  2:1119336/150‑1 (MQ=255)
                                                                                                              aTTCGATTAATTGCATATGTTTTCTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTg                                    <  1:1094829/150‑1 (MQ=255)
                                                                                                              aTTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTg                                    <  1:1662779/150‑1 (MQ=255)
                                                                                                              aTTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTg                                    <  1:3679508/150‑1 (MQ=255)
                                                                                                                   aTAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCaaaa                               <  1:3218510/150‑1 (MQ=255)
                                                                                                                       aTTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTAc                           <  1:3127360/150‑1 (MQ=255)
                                                                                                                         tGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCg                         <  1:1391534/150‑1 (MQ=255)
                                                                                                                          gCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGt                        <  2:4131044/150‑1 (MQ=255)
                                                                                                                           cATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCCAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGtt                       <  2:1529631/150‑1 (MQ=255)
                                                                                                                           cATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGtt                       >  1:1365798/1‑150 (MQ=255)
                                                                                                                           cATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGtt                       >  2:4790651/1‑150 (MQ=255)
                                                                                                                           cATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGtt                       >  2:3437074/1‑150 (MQ=255)
                                                                                                                            atatGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTa                      <  2:2583859/150‑1 (MQ=255)
                                                                                                                            atatGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTa                      <  2:3776883/150‑1 (MQ=255)
                                                                                                                             tatGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAc                     <  2:1452066/150‑1 (MQ=255)
                                                                                                                              atGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAcc                    <  1:4310267/150‑1 (MQ=255)
                                                                                                                              atGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAcc                    <  1:2928771/150‑1 (MQ=255)
                                                                                                                                gTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTg                  >  1:818601/1‑150 (MQ=255)
                                                                                                                                  ttACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGaa                >  1:2066880/1‑150 (MQ=255)
                                                                                                                                     cTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTa             <  2:263951/150‑1 (MQ=255)
                                                                                                                                      tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc            >  2:3916593/1‑150 (MQ=255)
                                                                                                                                      tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc            >  2:2311819/1‑150 (MQ=255)
                                                                                                                                      tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc            <  2:3251663/150‑1 (MQ=255)
                                                                                                                                      tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc            <  2:2043808/150‑1 (MQ=255)
                                                                                                                                      tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc            >  2:3916606/1‑150 (MQ=255)
                                                                                                                                         cGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGc         >  1:395558/1‑150 (MQ=255)
                                                                                                                                            tAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTc      >  1:2174179/1‑150 (MQ=255)
                                                                                                                                            tAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTc      >  1:5141833/1‑150 (MQ=255)
                                                                                                                                             aGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCt     >  1:541644/1‑150 (MQ=255)
                                                                                                                                             aGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCt     <  2:3483823/150‑1 (MQ=255)
                                                                                                                                             aGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCt     >  2:1629470/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTA  >  CP009273/1574376‑1574669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑